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Valentina Matiller Gustavo J. Hein Antonela F. Stassi Emmanuel Angeli Eduardo M. Belotti Hugo H. Ortega Florencia Rey Natalia R. Salvetti 《Reproduction in domestic animals》2019,54(1):46-54
The objective of this study was to examine the expression of transforming growth factor beta receptor (TGFBR)1, TGFBR2, TGFBR3, activin receptor (ACVR)1B and ACVR2B in ovaries of cows with cystic ovarian disease (COD). The expression of the selected receptors was determined by immunohistochemistry in sections of ovaries from cows with ACTH‐induced and spontaneous COD. Expression of TGFBR1 and TGFBR3 was higher in granulosa cells of cysts from cows with spontaneous COD than in tertiary follicles from the control group. Additionally, TGFBR3 expression was higher in granulosa cells of cysts from cows with ACTH‐induced COD than in those from the control group and lower in theca cells of spontaneous and ACTH‐induced cysts than in tertiary control follicles. There were no changes in the expression of TGFBR2. ACVR1B expression was higher in granulosa cells of tertiary follicles of cows with spontaneous COD than in the control group, whereas ACVR2B expression was higher in cysts of the spontaneous COD group than in tertiary follicles from the control group. The alterations here detected, together with the altered expression of the ligands previously reported, indicate alterations in the response of the ligands in the target cells, modifying their actions at cellular level. 相似文献
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Effect of Manuka honey gel on the transforming growth factor β1 and β3 concentrations,bacterial counts and histomorphology of contaminated full‐thickness skin wounds in equine distal limbs 
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JA Ruben Dal Sasso C NR Geist WJ Hillenius TD Jones M Signore 《Science (New York, N.Y.)》1999,283(5401):514-516
Ultraviolet light analysis of a fossil of the theropod dinosaur Scipionyx samniticus revealed that the liver subdivided the visceral cavity into distinct anterior pleuropericardial and posterior abdominal regions. In addition, Scipionyx apparently had diaphragmatic musculature and a dorsally attached posterior colon. These features provide evidence that diaphragm-assisted lung ventilation was present in theropods and that these dinosaurs may have used a pattern of exercise physiology unlike that in any group of living tetrapods. 相似文献
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Salvetti NR Ricci N Dallard BE Lorente JA Iguzquiza I Ortega HH 《Anatomia, histologia, embryologia》2000,29(3):129-134
Most studies on the biochemistry and structure of the corpus luteum have focused on elucidating the processes of progesterone synthesis and release. In the present work, the histochemical composition of the corpus luteum of the rat was evaluated using lectinhistochemistry on rats at the end of pregnancy (days 18-23). We also analysed the morphology of the luteal cells, to characterize the changes attributable to regression in this organ. Seven biotinylated lectins were used (CON-A, WGA, DBA, SBA, PNA, RCA and UEA-I) following pre-set protocols (ABC method). The average diameter and area of the cells and their nuclei were measured. High reactivity of the luteal cells was observed with CON-A and a lower reactivity with WGA. The capillary endothelium gave positive reactivity with WGA and to a certain extent with SBA, PNA and RCA. Vesicular structures were intensely stained with DBA, and were more abundant in sections from animals with more advanced pregnancy, which could be attributable to cellular debris, on the basis of their morphologic characteristics. There were no significant differences among the cytometric variables analysed in comparisons of the values corresponding to the different days of gestation. These observations, together with previous research, suggest that, on the day of delivery, the corpus luteum of the rat is in the very early stages of structural regression, with no changes at the morphological level, but with changes at the molecular level. 相似文献
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Ortega HH Palomar MM Acosta JC Salvetti NR Dallard BE Lorente JA Barbeito CG Gimeno EJ 《Research in veterinary science》2008,84(3):419-427
The objective of this research was to determine changes in IGF-I levels in serum and follicular fluid, and immunoreactivity of the follicle wall of cows with spontaneous (slaughter specimens) or ACTH-induced follicular cysts, and to compare results to normal cycling (control) cows after selection of the ovulatory follicle. Concentrations of IGF-I in serum did not differ between control and cystic animals (p=0.76). Fluid from the ovulatory follicle in control cows had 41% higher concentrations of IGF-I than that from cystic follicles collected at slaughter (spontaneous cysts; p<0.05) and 70% higher than that in induced follicular cysts (p<0.05). An intense positive immunostaining with anti-IGF-I was observed in granulosa cells (p<0.05) and in the theca interna (p<0.05) of secondary and tertiary follicles in all three groups of animals, but staining was less intense in cystic (p<0.05) and atretic follicles (p<0.05). This study provides evidence to suggest that cystic ovarian disease in cattle is associated with decreased concentrations of IGF-I in follicular fluid, but not in serum, and decreased production of IGF-I in the follicular wall. These data support the notion that IGF-I plays a role in the regulation of folliculogenesis, and may participate in the pathogenesis of cystic ovarian disease in cattle. 相似文献
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Generation of embryonic stem‐like cells from in vivo‐derived porcine blastocysts at a low concentration of basic fibroblast growth factor 下载免费PDF全文
下载免费PDF全文 NR Han H‐Y Kim S Baek S‐H Lee J Lee E Lee C‐K Park ST Lee 《Reproduction in domestic animals》2018,53(1):176-185
Although basic fibroblast growth factor (bFGF) is an essential factor supporting the maintenance of porcine embryonic stem (ES) cell self‐renewal and pluripotency, its high cost has limited previous studies, and the development of a low‐cost culture system is required. For these systems, in vivo blastocysts were progressively cultured under various conditions consisting of different culture mediums and/or different feeder cell numbers at a low concentration of bFGF. As the results, the sequential culture of in vivo‐derived porcine blastocysts on 5.0 × 105 mouse embryonic fibroblast (MEF) feeder cells in alpha minimum essential medium‐based medium for primary culture, on 2.5 × 105 MEF feeder cells in Mixture medium for the 1st subpassage, and on 2.5 × 105 MEF feeder cells in DMEM/Ham's F10‐based medium for the post‐2nd subpassage could support the establishment and maintenance of porcine ES‐like cells at the low concentration of bFGF. The established porcine ES‐like cells showed ES cell‐specific characteristics such as self‐renewal and pluripotency. We confirmed that porcine ES‐like cells could be generated from in vivo‐derived porcine blastocysts at a low concentration of bFGF. 相似文献
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The concentrations of cadmium (Cd), lead (Pb), chromium (Cr), copper (Cu), zinc (Zn), and iron (Fe) in water and selected fish species (Oreochromis nilzinc (oticus and Clarias gariepinus) from Benue-Niger River Confluence, Nigeria were investigated. The water and fish sample digests were quantified using an atomic absorption spectrophotometer. The study aimed to assess any possible human health risk associated with water and fish consumption from the selected geographical area. The concentration of the metals in the surface water ranged from 0.007 to 0.039, 0.106 to 0.240, 0.092 to 0.0164, 0.682 to 0.878, 0.058 to 0.309, and 0.136 to 1.645 mg/L for Cd, Cr, Cu, Fe, Pb, and Zn, respectively, while the concentrations of the metals, Cd, Pb, Cr, Cu, Zn, and Fe in these fish species ranged from 0.034 to 0.679, 0.500 to 1.75, 0.152 to 0.351, 0.36 to 1.01, 5.32 to 7.41, and 4.25 to 9.17 mg/kg, respectively. Cd, Cr, and Pb levels in fish were higher than the provisional tolerable weekly intake of 7, 1.5, and 25 μg/kg bw, respectively, according to the World Health Organization. Dry season values of the metals in water samples were higher, and sharptooth catfish had higher amounts of the metals. The hazard quotient (HQ) for Cd indicated a possible potential health concern with fish consumption from this area. 相似文献