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排序方式: 共有174条查询结果,搜索用时 31 毫秒
51.
Yuna Zayasu Takeshi Takeuchi Tomofumi Nagata Megumi Kanai Manabu Fujie Mayumi Kawamitsu Wakana Chinen Chuya Shinzato Noriyuki Satoh 《水产资源保护:海洋与淡水生态系统》2021,31(9):2429-2439
- It is essential to consider genetic composition for both conventional coral restoration management and for initiating new interventions to counter the significant global decline in living corals. Population genetic structure at a fine spatial scale should be carefully evaluated before implementing strategies to achieve self-sustaining ecosystems via coral restoration.
- This study investigated the population genetic structure of two acroporid species at Kume Island, Okinawa, Japan. There were 140 colonies of Acropora digitifera collected from seven study sites, and 81 colonies of Acropora tenuis from six sites. In total, 384 single nucleotide polymorphism (SNP) loci for A. digitifera and 470 SNPs for A. tenuis were obtained using a comparatively economical technique, Multiplexed ISSR Genotyping by sequencing.
- Observed heterozygosity was significantly lower than expected heterozygosity at all SNP sites in both acroporid species, suggesting deficient genetic diversity possibly caused by past massive coral bleaching. Even though both species are broadcast spawners, the population structure was different in the two species. No detectable structure was evident in A. digitifera, but two distinct clades were found in A. tenuis. The genetic homogeneity of A. digitifera at Kume Island suggests that this species could be used as a focal species for active restoration in terms of genetic differentiation at this island. By contrast, A. tenuis unexpectedly included two distinct clades with little or no admixture within a small study area, possibly representing two reproductively isolated cryptic species. Thus, when using A. tenuis, it would be prudent to avoid disturbing the genetic composition of wild populations until this question is answered.
52.
Karine P. Gendron Elizabeth W. Howerth Koichi Nagata Michael Perlini Nicole Northrup 《The Canadian veterinary journal. La revue veterinaire canadienne》2021,62(8):857
Two adult neutered male dogs were presented for evaluation of firm, painless masses arising within muscle: an 8-year-old German wirehaired pointer dog with an accessory tricipital growth, and a 3-year-old German shepherd dog with a gracilis muscle growth. Magnetic resonance imaging (MRI) characteristics suggested malignant behavior, with a central fluid-like portion with a hyperenhancing lining, a nidus of disorganized tissue, and an extensive reactive zone, whereas histopathology was consistent with low-grade fibrosarcoma. This report describes histologically low-grade, yet biologically high-grade intramuscular fibrosarcoma, in which MRI provided detailed information on tumor behavior and assisted with biopsy and surgical planning. 相似文献
53.
G Trakoontivakorn K Nakahara H Shinmoto M Takenaka M Onishi-Kameyama H Ono M Yoshida T Nagata T Tsushida 《Journal of agricultural and food chemistry》2001,49(6):3046-3050
Six compounds were isolated from fresh rhizomes of fingerroot (Boesenbergia pandurata Schult.) as strong antimutagens toward 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) in Salmonella typhimurium TA98. These compounds were 2',4',6'-trihydroxychalcone (pinocembrin chalcone; 1), 2',4'-dihydroxy-6'-methoxychalcone (cardamonin; 2), 5,7-dihydroxyflavanone (pinocembrin; 3), 5-hydroxy-7-methoxyflavanone (pinostrobin; 4), (2,4,6-trihydroxyphenyl)-[3'-methyl-2'-(3' '-methylbut-2' '-enyl)-6'-phenylcyclohex-3'-enyl]methanone (5), and (2,6-dihydroxy-4-methoxyphenyl)-[3'-methyl-2'-(3' '-methylbut-2' '-enyl)-6'-phenylcyclohex-3'-enyl]methanone (panduratin A; 6). Compound 5 was a novel compound (tentatively termed 4-hydroxypanduratin A), and 1 was not previously reported in this plant, whereas 2-4 and 6 were known compounds. The antimutagenic IC(50) values of compounds 1-6 were 5.2 +/- 0.4, 5.9 +/- 0.7, 6.9 +/- 0.8, 5.3 +/- 1.0, 12.7 +/- 0.7, and 12.1 +/- 0.8 microM in the preincubation mixture, respectively. They also similarly inhibited the mutagenicity of 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2) and 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP). All of them strongly inhibited the N-hydroxylation of Trp-P-2. Thus, the antimutagenic effect of compounds 1-6 was mainly due to the inhibition of the first step of enzymatic activation of heterocyclic amines. 相似文献
54.
55.
Ando M Kado S Suzuki T Yamamoto R Nagata Y Hata C Uchida K Kaneko K 《Journal of toxicologic pathology》2011,24(4):251-255
A subcutaneous tumor was found in the right abdomen of a 16-week-old male TCRβ and p53 double-knockout mouse. The tumor had indistinct borders with the surrounding tissue. The cut surface after formalin fixation was pale yellowish white, partially dark red and partly white. Histologically, the tumor was composed of three distinct regions. The first region showed pleomorphic cells arranged in sheets. The second region showed spindle cells arranged in interlacing fascicles. The final region contained a mixture of the above mentioned two types of cells. Furthermore, a small amount of collagen fibers, round cells, multinucleated giant cells, and cells with eosinophilic granules were observed between these tumor cells. Immunohistochemical examination and electron microscopy identified that the pleomorphic cells and spindle cells were histiocytes and fibroblasts, respectively, and that the round cells were undifferentiated mesenchymal cells. Based on these findings, the tumor was diagnosed as a malignant fibrous histiocytoma. 相似文献
56.
Nagata N Yuki M Hasegawa T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(12):1585-1589
Intraocular pressure (IOP) evaluated by applanation tonometry via TONO-PEN XL (TP), and rebound tonometry via TonoVet (TV) were compared in enucleated canine eyes with varied pressure of the anterior chamber (AC) and in clinical cases. TV measured IOP values were lower than IOP measurements of TP in the enucleated eyes with 5-10 mmHg of AC (P<0.0001), though there was no significant difference in IOP values obtained with TP and TV on the pressure ranges of 15-20 mmHg. However, TP detected IOP values were lower than IOP measurements of TV in the eyes with over 25 mmHg of AC (P<0.0001). The results of clinical cases were similar to the enucleated eye model. There was no significant difference in IOP values obtained from TP and TV in dogs with normotensive eyes. IOP measurements of TP were lower than those of TV in glaucomatous eyes (P<0.0001). TV was a reliable tonometer for measurement of IOP in hypertensive eyes, whereas it was less accurate than TP in hypotensive eyes. The characteristics of TP and TV should be considered in the evaluation of IOP in practice. 相似文献
57.
Kashiyama Y Miyashita H Ohkubo S Ogawa NO Chikaraishi Y Takano Y Suga H Toyofuku T Nomaki H Kitazato H Nagata T Ohkouchi N 《Science (New York, N.Y.)》2008,321(5889):658
Although analyses of chlorophyll d (Chl d)-dominated oxygenic photosystems have been conducted since their discovery 12 years ago, Chl d distribution in the environment and quantitative importance for aquatic photosynthesis remain to be investigated. We analyzed the pigment compositions of surface sediments and detected Chl d and its derivatives from diverse aquatic environments. Our data show that the viable habitat for Chl d-producing phototrophs extends across salinities of 0 to 50 practical salinity units and temperatures of 1 degrees to 40 degrees C, suggesting that Chl d production can be ubiquitously observed in aquatic environments that receive near-infrared light. The relative abundances of Chl d derivatives over that of Chl a derivatives in the studied samples are up to 4%, further suggesting that Chl d-based photosynthesis plays a quantitatively important role in the aquatic photosynthesis. 相似文献
58.
Terminally misfolded proteins in the endoplasmic reticulum (ER) are retrotranslocated to the cytoplasm and degraded by proteasomes through a mechanism known as ER-associated degradation (ERAD). EDEM, a postulated Man8B-binding protein, accelerates the degradation of misfolded proteins in the ER. Here, EDEM was shown to interact with calnexin, but not with calreticulin, through its transmembrane region. Both binding of substrates to calnexin and their release from calnexin were required for ERAD to occur. Overexpression of EDEM accelerated ERAD by promoting the release of terminally misfolded proteins from calnexin. Thus, EDEM appeared to function in the ERAD pathway by accepting substrates from calnexin. 相似文献
59.
A liquid chromatographic (LC) method is described for determination of spiramycin residues in chicken muscles. The drug is extracted from muscles with acetonitrile, the extract is concentrated to 3-4 mL and rinsed with n-hexane followed by ethyl ether, and the drug is extracted with chloroform. LC analysis is carried out on a Zorbax BP-C8 column, and spiramycin is detected spectrophotometrically at 231 nm. Recoveries of spiramycin added to chicken muscles at 0.2 and 0.1 ppm were 93.9 and 89.0%, respectively. The detection limit was 5 ng for spiramycin standard, and 0.05 ppm in chicken muscles. 相似文献
60.
Tomoaki Morishita Ryusuke Hatano Osamu Nagata Kohei Sakai Takahiro Koide Osamu Nakahara 《Soil Science and Plant Nutrition》2013,59(8):1187-1194
Abstract It has been well documented by short-term artificial experiments that the CH4 uptake is inhibited by N input, especially NH4 p+-N input. To investigate the effect of the natural N input by throughfall and other factors on the CH4 uptake in forest soils, we measured the CH4 uptake rates for 6 months during the snow-free period of the year and N input by throughfall throughout the year at 10 sites in Hokkaido, Japan, from 1997 to 2002. Water filled pore space (WFPS) and pH values in the soils varied widely among the sites (38-93% and 3.9-6.2, respectively). The rates of NH4 p+-N and NH3 p--N inputs ranged from 1.3 to 6.9 kg N hap-1 yearp-1 and from 0.8 to 2.9 kg N hap-1 yearp-1, respectively. The NH4 p+-N input was generally higher than the NH3 p--N input. Total N input by throughfall amounted to 2.3-9.4 kg N hap-1 yearp-1. The highest CH4 uptake rate occurred within the period from July to September (41-215 μg CH4 mp-2 hp-1) each year at most sites. CH4 uptake rate was relatively low (~50 μg CH4 M-2 hp-1) at northern sites, while a high CH4 uptake rate was observed throughout the year 100 (? CH4 mp-2 hp-1) at southern sites. The mean CH4 uptake rates were significantly different among the sites. Cumulative CH4 uptake ranged from 1.4 to 6.6 kg CH4 hap-1 [184 d]p-1 with a mean values of 3.22 ± 1.36 kg CH4 hap-1 [184 d]p-1. Cumulative CH4 uptake increased with increasing temperature and decreased with an increase in precipitation (Rain), NH4 p+-N input (TFNH4) WFPS, soil total C (TC), and total N (TN). There was a quadratic relationship between the CH4 uptake and NH3 p--N input (TFNO3), soil pH, and C / N ratio in soil. A regression equation was obtained as follows to predict the CH4 uptake in forest soils: Cumulative CH4 uptake = 0.47 / Rain + 0.38 / TFNH4 + 0.34 / TC - 0.30 / TFN03 (R p2 = 0.74, p = 0.0001). This equation indicates that atmospheric N input into forest soils is one of the main factors that control cumulative CH4 uptake with precipitation, total carbon content in soil in Hokkaido, Japan. 相似文献