In vitro fertilization of follicular oocytes from the swamp buffalo (Bubalus bubalis) and Kedah-Kelantan cattle (Bos indicus) in Malaysia.     

Y Takahashi  M Nihayah  M R Jainudeen  M Hishinuma  Y Kanai  Y Mori  H Kanagawa 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1992,54(4):799-801

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Testicular dynamics in Syrian hamsters exposed to both short photoperiod and low ambient temperature     

Sato T  Tachiwana T  Takata K  Tay TW  Ishii M  Nakamura R  Kimura S  Kanai Y  Kurohmaru M  Hayashi Y 《Anatomia, histologia, embryologia》2005,34(4):220-224

The object of this study was to determine the details of morphological dynamics of spermatogenesis in Syrian hamsters exposed to both short photoperiod and low ambient temperature. Eight-week-old male hamsters, kept in a long photoperiod (14 h L, 10 h D), were transferred to a short photoperiod (6 h L, 18 h D) and kept there for 13 weeks to induce testicular regression. Some hamsters were then transferred from the room at 23 degrees C to that at 5 degrees C (5 degrees C group). Remaining hamsters were continuously kept at 23 degrees C (23 degrees C group). Thereafter, the morphology was examined. As a result, it took only 8 weeks until spermatogenesis recovered in the 23 degrees C group. However, it was not until 20 weeks that spermatogenesis was recognized in the 5 degrees C group. As the regulation of seasonal testicular activity is characterized by coordinated shifts in the relationships among mitosis, meiosis, and apoptosis, the changes in the proliferative and apoptotic activities were examined. Although no significant difference in proliferative activity of spermatogonia between the 5 degrees C and the 23 degrees C groups was confirmed, a notable increase in the rate of apoptosis was observed in the 5 degrees C group. Furthermore, this increase was more salient during the hibernation period. These findings suggest that both cold ambient temperature and hibernation caused the delay of testicular recrudescence and this delay arose from the increase of apoptotic activity but not the change in proliferative activity in spermatogonia in the 5 degrees C group.  相似文献   

Prevalence of Giardia intestinalis and other zoonotic intestinal parasites in private household dogs of the Hachinohe area in Aomori prefecture, Japan in 1997, 2002 and 2007     

Naoyuki Itoh  Kazutaka Kanai  Yasutomo Hori  Fumio Hoshi  Seiichi Higuchi 《Journal of veterinary science (Suw?n-si, Korea)》2009,10(4):305-308

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Di(n‐butyl) Phthalate Induces Vimentin Filaments Disruption in Rat Sertoli Cells: A Possible Relation with Spermatogenic Cell Apoptosis     

M. S. Alam  S. Ohsako  T. W. Tay  N. Tsunekawa  Y. Kanai  M. Kurohmaru 《Anatomia, histologia, embryologia》2010,39(3):186-193

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Cycle of the seminiferous epithelium in the Java fruit bat (Pteropus vampyrus) and the Japanese lesser horseshoe bat (Rhinolophus cornutus)     

Morigaki T  Kurohmaru M  Kanai Y  Mukohyama M  Hondo E  Yamada J  Agungpriyono S  Hayashi Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(7):773-779

The cycle of the seminiferous epithelium in the Java fruit bat, Pteropus vampyrus, and the Japanese lesser horseshoe bat, Rhinolophus cornutus, was investigated by light microscopy and the characteristics of spermiogenesis were compared between these two species. In the Java fruit bat, the cycle of the seminiferous epithelium was divided into 11 stages and developing spermatids were subdivided into 13 steps. While in the Japanese lesser horseshoe bat, the cycle of the seminiferous epithelium was divided into 10 stages and developing spermatids were subdivided into 13 steps. Excepting slight morphological differences, the characteristics of acrosomal formation in both species were almost similar with each other. In the Java fruit bat after stage VII, the acrosome gradually elongated, flattened and finally became scoop-like in shape. In the Japanese lesser horseshoe bat after stage VIII, the acrosome elongated, flattened and then slightly shortened. Before spermiation, the acrosome became long spatula-like in shape. The elongation and flattening of spermatids in these two species were similar to those in insectivores. The finding may reflect the fact that the order Chiroptera is phylogenetically close to the order Insectivora.  相似文献   

Laparoscopic cholecystectomy using the subserosal layer dissection technique in dogs: 34 cases (2015-2021)     

M. Kondo  K. Hagiwara  A. Nukaya  T. Aso  H. Kanai 《The Journal of small animal practice》2023,64(4):288-295

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Diode laser coagulation for the treatment of epistaxis in a Scottish fold cat     

Takuma Aoki  Hiroo Madarame  Keisuke Sugimoto  Hiroshi Sunahara  Yoko Fujii  Eiichi Kanai  Tetsuro Ito 《The Canadian veterinary journal. La revue veterinaire canadienne》2015,56(7):745-748

We report the case of a 4-year-old, castrated 4.2-kg Scottish fold cat with recurrent epistaxis that was unresponsive to medical therapy. Diathermocoagulation of the nasal mucosa with a diode laser controlled the epistaxis and there was no significant recurrence of epistaxis during 1 year of follow-up.  相似文献   

Inhibitory effect of açaí (Euterpe oleracea Mart.) pulp on IgE-mediated mast cell activation     

Horiguchi T  Ishiguro N  Chihara K  Ogi K  Nakashima K  Sada K  Hori-Tamura N 《Journal of agricultural and food chemistry》2011,59(10):5595-5601

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Anti-inflammatory potency of oral disulfiram compared with dexamethasone on endotoxin-induced uveitis in rats     

Kanai K  Itoh N  Ito Y  Nagai N  Hori Y  Chikazawa S  Hoshi F  Higuchi S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(4):517-520

To investigate potency of oral disulfiram (DSF) compared with that of dexamethasone (Dexa), on endotoxin-induced uveitis (EIU) in rats. The oral administration with 750 mg/kg DSF suppressed the number of inflammatory cells, protein concentration, and levels of tumor necrosis factor (TNF)-α, Nitric oxide (NO) and prostaglandin (PG) E2 in the aqueous humor and improved the histiologic status of the ocular tissue at 24 hr after lipopolysaccharide (LPS) injection. The anti-inflammatory potency of DSF oral administration was as strong as that observed with 0.5 mg/kg Dexa in the present study. The results suggest that DSF might pave the way for a novel therapeutic agent for the management of uveitis.  相似文献   

Hyperbaric air therapy in dogs for clinical veterinary medicine: a basic study     

Yanagisawa H  Kanai E  Kayanuma H  Shida T  Suganuma T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(10):1351-1354

Hyperbaric air therapy (HBA) is a treatment in which an animal is exposed to air pressurized to about 1.3 atmosphere absolute (ATA). Although HBA has already been administered to humans in medical applications, it has not been reported in clinical veterinary medicine. Therefore, we aimed to determine a safe protocol for dogs. To elucidate oxygen dynamics during HBA, we measured partial pressure of arterial oxygen, oxygen saturation of tissue, and partial pressure of transcutaneous oxygen in dogs. HBA could be performed safely with a protocol of pressurizing speed up to 0.1 ATA/min, maximum chamber pressure of up to 1.3 ATA, and pressure duration of around 45 min per treatment. Under these conditions, tissue was adequately oxygenated during and after treatment.  相似文献