Photodynamic hyperthermal therapy with indocyanine green (ICG) induces apoptosis and cell cycle arrest in B16F10 murine melanoma cells     

Radzi R  Osaki T  Tsuka T  Imagawa T  Minami S  Nakayama Y  Okamoto Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(5):545-551

We examined the effects of photodynamic hyperthemal therapy (PHT), which is a combination of photodynamic therapy (PDT) and hyperthermia (HT), on the apoptosis and cell cycle progression of murine melanoma B16F10 cells. The percentage of apoptotic cell was determined by flow cytometry using fluorescein isothiocyanate (FITC)-conjugated Annexin V and propidium iodide (PI) double staining. The cell cycle analysis was performed by PI staining with flow cytometry. The expression of cyclins and heat shock protein 70 (Hsp70) were examined by a Western blotting analysis. PHT induces death in B16F10 cells, and PHT-mediated apoptosis occurred acutely and persistently in vitro. Our study demonstrated that PHT using indocyanine green (ICG) and near infrared (NIR) light source induces apoptosis and G0/G1 cell cycle arrest in the B16F10 cells.  相似文献   

Heterotrophic respiration causes seasonal hysteresis in soil respiration in a warm-temperate forest     

Yuji Kominami  Mayuko Jomura  Mioko Ataka  Koji Tamai  Takafumi Miyama  Masako Dannoura  Naoki Makita  Kenichi Yoshimura 《Journal of Forest Research》2012,17(3):296-304

To assess the effect of changes in organic litter stock on seasonal changes in heterotrophic respiration (R _H), soil respiration (R _S), and total ecosystem respiration (R _E), we measured seasonal changes in leaf litter respiration (R _LL) by the chamber method and estimated the seasonal change in total R _H using the RothC model in a warm-temperate mixed deciduous?Cevergreen forest in Japan. Both R _E and R _S had seasonal hysteresis and were higher in spring than at the same temperature during autumn. Under warm and humid conditions, the rate of decomposition of newly supplied leaf litter in one?year was high (60% loss). Consequently, R _LL and R _H were higher in spring after leaf drop, when more fresh material was available, than in autumn. In this study, 42 and 88% of the difference in R _E and R _S between spring and autumn (soil temperature 16?C18°C) could be accounted for by the difference in R _H, respectively, and 71% of the difference in R _H could be accounted for by the difference in R _LL. This study showed that seasonal changes in heterotrophic respiration (R _LL and R _H) could be a major factor in the seasonal hysteresis of R _E and R _S.  相似文献   

A simple and efficient transient transformation for hybrid aspen (Populus tremula × P. tremuloides)     

N Takata  ME Eriksson 《Plant methods》2012,8(1):30

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Use of image analysis to estimate anthocyanin and UV-excited fluorescent phenolic compound levels in strawberry fruit     

Yosuke Yoshioka  Masayoshi Nakayama  Yuji Noguchi  Hideki Horie 《Breeding Science》2013,63(2):211-217

Strawberry is rich in anthocyanins, which are responsible for the red color, and contains several colorless phenolic compounds. Among the colorless phenolic compounds, some, such as hydroxycinammic acid derivatives, emit blue-green fluorescence when excited with ultraviolet (UV) light. Here, we investigated the effectiveness of image analyses for estimating the levels of anthocyanins and UV-excited fluorescent phenolic compounds in fruit. The fruit skin and cut surface of 12 cultivars were photographed under visible and UV light conditions; colors were evaluated based on the color components of images. The levels of anthocyanins and UV-excited fluorescent compounds in each fruit were also evaluated by spectrophotometric and high performance liquid chromatography (HPLC) analyses, respectively and relationships between these levels and the image data were investigated. Red depth of the fruits differed greatly among the cultivars and anthocyanin content was well estimated based on the color values of the cut surface images. Strong UV-excited fluorescence was observed on the cut surfaces of several cultivars, and the grayscale values of the UV-excited fluorescence images were markedly correlated with the levels of those fluorescent compounds as evaluated by HPLC analysis. These results indicate that image analyses can select promising genotypes rich in anthocyanins and fluorescent phenolic compounds.  相似文献   

Changes in the bacterial community and composition of fermentation products during ensiling of wilted Italian ryegrass and wilted guinea grass silages     

Yanbing Li  Naoki Nishino 《Animal Science Journal》2013,84(8):607-612

To gain further insights into temperate and tropical grass ensiling, fermentation products and bacterial communities were examined at both the initial and late stages of ensiling of wilted Italian ryegrass and wilted guinea grass silages. 2,3‐Butanediol and ethanol fermentation were observed in wilted Italian ryegrass silage. Enterobacteria such as Rahnella sp. and Enterobacter sp. may have been involved in fermentation; however, alcohol production was intensified after the silage enterobacterial community overwhelmed the pre‐ensiled enterobacterial community. Pediococcus spp. appeared in silage stored for 4 months, when a significant increase in lactic acid content was seen compared with that at 2 months. Prolonged storage enhanced acetic acid fermentation in wilted guinea grass silage. The disappearance of Enterococcus sulfureus and appearance of Lactobacillus plantarum may have been associated with the increased acetic acid content. Although many species of enterobacteria were found in common between the pre‐ensiled crop and silages of Italian ryegrass and guinea grass, marked differences were seen in the type of fermentation from the initial stages. These results indicate that the bacterial community of pre‐ensiled crops may be immediately replaced by one that is adapted to ensiling environments, although metabolic changes may continue over the course of ensiling.  相似文献   

Intraocular vaccination with an inactivated highly pathogenic avian influenza virus induces protective antibody responses in chickens     

Hirokazu Hikono  Masaji Mase  Aya Matsuu  Megumi Nakayama  Takehiko Saito 《Veterinary immunology and immunopathology》2013,151(1-2):83-89

Because it is expected to induce cross-reactive serum and mucosal antibody responses, mucosal vaccination against highly pathogenic avian influenza (HPAI) is potentially superior to conventional parenteral vaccination. Here, we tested whether intraocular vaccination with an inactivated AI virus induced protective antibody responses in chickens. Chickens were inoculated intraocularly twice with 10⁴ hemagglutination units of an inactivated H5N1 HPAI virus. Four weeks after the second vaccination, the chickens were challenged with a lethal dose of the homologous H5N1 HPAI virus. Results showed that most of the vaccinated chickens mounted positive antibody responses. The median serum hemagglutination inhibition titer was 1:80. Addition of CpG oligodeoxynucleotide 2006 or cholera toxin to the vaccine did not enhance serum antibody titers. Cross-reactive anti-hemagglutinin IgG, but not IgA, was detected in oropharyngeal secretions. In accordance with these antibody results, most vaccinated chickens survived a lethal challenge with the H5N1 HPAI virus and did not shed the challenge virus in respiratory or digestive tract secretions. Our results show that intraocular vaccination with an inactivated AI virus induces not only systemic but also mucosal antibody responses and confers protection against HPAI in chickens.  相似文献   

Glycosylated Porphyra-334 and Palythine-Threonine from the Terrestrial Cyanobacterium Nostoc commune     

Ehsan Nazifi  Naoki Wada  Minami Yamaba  Tomoya Asano  Takumi Nishiuchi  Seiichi Matsugo  Toshio Sakamoto 《Marine drugs》2013,11(9):3124-3154

Mycosporine-like amino acids (MAAs) are water-soluble UV-absorbing pigments, and structurally different MAAs have been identified in eukaryotic algae and cyanobacteria. In this study novel glycosylated MAAs were found in the terrestrial cyanobacterium Nostoc commune (N. commune). An MAA with an absorption maximum at 334 nm was identified as a hexose-bound porphyra-334 derivative with a molecular mass of 508 Da. Another MAA with an absorption maximum at 322 nm was identified as a two hexose-bound palythine-threonine derivative with a molecular mass of 612 Da. These purified MAAs have radical scavenging activities in vitro, which suggests multifunctional roles as sunscreens and antioxidants. The 612-Da MAA accounted for approximately 60% of the total MAAs and contributed approximately 20% of the total radical scavenging activities in a water extract, indicating that it is the major water-soluble UV-protectant and radical scavenger component. The hexose-bound porphyra-334 derivative and the glycosylated palythine-threonine derivatives were found in a specific genotype of N. commune, suggesting that glycosylated MAA patterns could be a chemotaxonomic marker for the characterization of the morphologically indistinguishable N. commune. The glycosylation of porphyra-334 and palythine-threonine in N. commune suggests a unique adaptation for terrestrial environments that are drastically fluctuating in comparison to stable aquatic environments.  相似文献   

Transcription profile of chemokine receptors,cytokines and cytotoxic markers in peripheral blood of dogs with epitheliotropic cutaneous lymphoma     

Naoki Chimura  Aki Iio  Eiji Ozaki  Takashi Mori  Yusuke Ito  Nobuo Murayama  Masahiko Nagata  Kaori Ide  Koji Nishifuji  Hiroaki Kamishina  Sadatoshi Maeda 《Veterinary dermatology》2013,24(6):628-e155

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Soil carbon budget in a single-cropping paddy field with rice straw application and water management based on soil redox potential   总被引：1，自引：0，他引：1  

Kazunori MINAMIKAWA  Naoki SAKAI 《Soil Science and Plant Nutrition》2007,53(5):657-667

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Retrospective diagnosis of feline GM2 gangliosidosis variant 0 (Sandhoff-like disease) in Japan: possible spread of the mutant allele in the Japanese domestic cat population     

Yamato O  Hayashi D  Satoh H  Shoda T  Uchida K  Nakayama H  Sakai H  Masegi T  Murai A  Iida T  Hisada H  Hisada A  Yamasaki M  Maede Y  Arai T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(8):813-818

GM2 gangliosidosis variant 0 (human Sandhoff disease) is a lysosomal storage disease caused by simultaneous deficiencies of acid beta-hexosaminidase (Hex) A and Hex B due to an abnormality of beta-subunit, a common component in these enzyme molecules, which is coded by the HEXB gene. In the present study, a retrospective diagnosis was performed in 2 previous suspected cases of feline Sandhoff-like disease using a DNA test to detect the causative mutation identified previously in 4 cats in 2 other families of Japanese domestic cats. Enzymic analysis was also performed using stored leukocytes and plasma collected from the subject families in order to investigate the usefulness of enzymic diagnosis and genotyping of carriers. The DNA test suggested that the 2 cases were homozygous recessive for the mutation. Consequently, 6 cats homozygous for the same mutation have been found in 4 separate locations of Japan, suggesting that this mutant allele may be spread widely in the Japanese domestic cat populations. In enzymic analysis, Hex A and Hex B activities in leukocytes and plasma measured using 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide as a substrate were negligible in affected cats, compared with those in normal and carrier cats. However, there was a wide overlap in enzyme activity between normal and carrier cats. Therefore, it was concluded that enzymic analysis is useful for diagnosis of affected cats, but is not acceptable for genotyping of carriers.  相似文献