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11.
Effect of interleukin‐1β on occludin mRNA expression in the duodenal and colonic mucosa of dogs with inflammatory bowel disease 
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Dung Thi LE Son Vu NGUYEN Mari OKAMOTO Nanako YAMASHITA-KAWANISHI Tung Duy DAO Vuong Nghia BUI Haruko OGAWA Kunitoshi IMAI Takeshi HAGA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(8):1273
The detection of bovine foamy virus (BFV) in Vietnamese cattle was performed using conventional PCR targeting pol and gag genes. Out of 243 tested samples, ten (4.1%) and eight (3.3%) samples were positive for BFV gag and pol DNA, respectively. The prevalence of bovine leukemia virus (BLV) estimated by detection of proviral DNA using nested PCR targeting env gene was 26.7% (65/243). The results of nucleotide sequence alignment and the phylogenetic analysis suggested that Vietnamese BFV strains showed high homology to isolates belonging to either European or non-European clades. There was no significant correlation between BLV and BFV. This study provides information regarding BFV infection and confirms the existence of two BFV clades among Vietnamese cattle for the first time. 相似文献
13.
In order to clarify the growth and reproductive success of seed‐derived Sagittaria trifolia individuals emerging at different times within a season, seeds were sown on May 16, June 16 and July 22 2010 and allowed to grow in pots under shaded and unshaded conditions. The timing of when the first inflorescence of each seedling appeared was recorded, as well as the number of seeds and tubers produced and the dry weight of each organ. In total, 220 seedlings were used, 23–59 in each treatment. As the sowing time was delayed, so too was the appearance of the first inflorescence. Additionally, the number of inflorescences, fruits and seeds produced by the unshaded plants significantly decreased with the sowing time. The total dry weight was significantly greater in the unshaded than in the shaded plants: the July‐sown, shaded plants grew very poorly. The relative resource allocation to the tubers was greater in the late‐emerging, shaded seedlings. Even in the shaded groups, except for the July‐sown plants, most of the plants set seed. Therefore, when addressing weed control in paddy fields, one should keep seedlings in mind, in addition to plants that have grown from tubers. 相似文献
14.
Waleed SENOSY Alaa Eldin ZAIN Abdel‐Razek Khalifa ABDEL‐RAZEK Michiko UCHIZA Nanae TAMEOKA Yoshiaki IZAIKE Takeshi OSAWA 《Animal Science Journal》2012,83(4):284-290
High‐yielding Holstein‐Friesian recipients (n = 43) were used in order to investigate the relation between energy balance status during the early postpartum period and subsequent embryonic mortality after transferring good‐quality frozen embryos. Blood samples were collected during the second, third, fourth, fifth, sixth and seventh weeks postpartum in order to measure energy status indicators. These indicators include β hydroxyl butyric acid (BHBA), non‐esterified fatty acid (NEFA), total cholesterol (T‐chol), glucose and blood urea nitrogen (BUN). Moreover, body condition scores (BCS) were assessed during the same period. Pregnancy diagnosis by ultrasonography at the 28th day postestrus and embryo viability was ascertained until 45 days postestrus in order to detect late embryonic mortality (LEM). The pregnancy rate on day 28 was 44.2% (19/43); however, five cows (11.6%) experienced LEM by day 45. Based on the non‐return rate at day 24, non‐pregnant animals, as diagnosed by ultrasonography, were allocated into animals with longer estrus intervals than 24 days (32.5%; mid‐embryonic mortality (MEM) group) and animals returning to estrus by day 24 postestrus (23.5%; early embryonic mortality (EEM) group). At week 5 postpartum, BCS was significantly (P < 0.05) lower in the LEM group than that of pregnant (PREG), EEM and MEM groups. NEFA was significantly higher in animals that experienced LEM (LEM group) at week 7 postpartum (289.6 ± 47.0 µEq/L; P < 0.01) than that of PREG (196.8 ± 16.0 µEq/L), EEM (157.2 ± 18.6 µEq/L) and MEM groups (191.5 ± 14.4 µEq/L). In conclusion, lower BCS at week 5 postpartum and higher NEFAs at week 7 postpartum may be associated with subsequent LEM in high‐yielding recipient cows. 相似文献
15.
Masahiro Shishido Nanako Yoshida Toshiyuki Usami Tetsuo Shinozaki Masanobu Kobayashi Taeko Takeuchi 《Journal of General Plant Pathology》2006,72(4):220-227
Although the causal agent of black root rot of Cucurbitaceae in Japan has been proposed as Phomopsis sclerotioides, the species identification of the pathogen has remained inconclusive because of a lack of spore formation. We confirmed
that a Japanese isolate of Phomopsis sp. obtained from a diseased pumpkin root produced pycnidia containing α spores in sterilized bean pods. In phylogenetic
analyses of rDNA-ITS regions, nine Japanese Phomopsis sp. isolates from melon, watermelon grafted onto bottle gourd, and pumpkin diagnosed with black root rot, formed a single
clade with P. sclerotioides standard isolates. We identified the causal agent of the black root rot of melon, pumpkin, bottle gourd, and watermelon in
Japan as P. sclerotioides and propose the Japanese name “Phomopsis-negusare-byo” for the disease. Patterns of random amplified polymorphic DNA (RAPD) of these Japanese isolates were also similar
to those of P. sclerotioides, thus supporting the species identification. However, mycelial incompatibilities were found for many combinations among these
P. sclerotioides isolates, suggesting some genotypic variations of this fungus in Japan at a level that the RAPD analyses cannot discriminate.
The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under the accession numbers AB201430
to AB201444 相似文献
16.
Yasuyuki KANEKO Shidow TORISU Go KITAHARA Yuichi HIDAKA Hiroyuki SATOH Taketoshi ASANUMA Shinya MIZUTANI Takeshi OSAWA Kiyokazu NAGANOBU 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(5):631-635
Laparoscopic cryptorchidectomy without insufflation was applied in 10 standing bulls aged
3 to 15 months. Nine bulls were preoperatively pointed out intra-abdominal testes by
computed tomography. Preoperative fasting for a minimum of 24 hr provided laparoscopic
visualization of intra-abdominal area from the kidney to the inguinal region. Surgical
procedure was interrupted by intra-abdominal fat and testis size. It took 0.6 to 1.5 hr in
4 animals weighing 98 to 139 kg, 0.8 to 2.8 hr in 4 animals weighing 170 to 187 kg, and 3
and 4 hr in 2 animals weighing 244 and 300 kg to complete the cryptorchidectomy. In
conclusion, standing gasless laparoscopic cryptorchidectomy seems to be most suitable for
bulls weighing from 100 to 180 kg. 相似文献
17.
Nanako USHIO Ken-ichi WATANABE James K. CHAMBERS Tokuhiro SHIBATO Hiroyuki NAKAYAMA Kazuyuki UCHIDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1523-1526
A rock pigeon (Columba livia) caught in Akihabara, Tokyo, showed
neurological symptoms, such as head tilt and circling. Pathological examinations revealed
abundant Sarcocystic cysts in the skeletal muscle and myocardium with mild myositis, and
numerous schizonts and sarcocysts with severe multifocal granulomatous T-lymphocytic
infiltration in the central nervous system. A Sarcocystis
calchasi-specific gene was detected in the muscle and brain. This case indicates
S. calchasi was distributed in Japan and caused severe encephalitis to
rock pigeons. 相似文献
18.
Takeshi OSAWA 《The Journal of reproduction and development》2021,67(5):291
A certain level of endometrial bacterial infection and inflammation is involved in bovine uterine involution during the puerperal period. Factors that hamper normal uterine involution expose the uterine environment to pathological conditions, causing different endometritis levels. The lack of proper diagnostic tools extends the time to conception. Efforts have been made to elucidate the postpartum uterine environment, including bacterial flora, changes in transient endometrial inflammation, and the pathophysiology of endometritis, to improve bovine reproductive performance. E. coli and Trueperella pyogenes in the uterus are likely to cause persistent infection, and Mycoplasma bovigenitalium infection is associated with dystocia and cytological endometritis in postpartum dairy cows. Due to the widespread use of cytobrush as a diagnostic tool for bovine subclinical endometritis (SE) that enables quantification of the degree of inflammation, we found that endometritis at week 5 postpartum was associated with delayed first ovulation. Approximately 30% of open cows have SE during the postpartum period, and cows with low blood glucose during prepartum have a high risk of developing SE. Additionally, cows with purulent vaginal discharge do not always have endometritis but only vaginitis and/or cervicitis. Intrauterine infusion of polyvinylpyrrolidone-iodine (PVP-I) improves fertility and promotes endometrial epithelial cell regeneration after inducing transient uterine inflammation, suggesting that PVP-I could be a good alternative to antibiotics. In conclusion, prepartum management to prevent glucose deficiency, prompt diagnosis to identify causative agents and intrauterine inflammation levels, and appropriate treatment to minimize antimicrobial resistance is beneficial for tackling endometritis and improving reproductive performance in bovine herds. 相似文献
19.
Mari OKAMOTO Keisuke OGUMA Nanako YAMASHITA-KAWANISHI Toshihiro ICHIJO Shinichi HATAMA Maiko ENDO Maya ISHIKAWA Takeshi HAGA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2020,82(11):1607
Bovine foamy virus (BFV) is distributed through worldwide cattle herds. Although the biological features of BFV are not well understood, appearance of clinical manifestation by superinfection with other microorganisms is inferred. In Japan, reports of genomic characterizations and epidemiology of this virus are limited. In this study, we performed whole genomic sequencing of BFV strains Ibaraki and No.43, which were isolated in this country. Additionally, we investigated BFV in geographically distant four daily farms in Japan, to estimate the distribution of BFV and its correlation to bovine leukemia virus (BLV). BFV was distributed throughout Japan; the average positive rate was 12.7%. The nucleotide sequence identities of the isolates were 99.6% when compared with BFV strain isolated in the USA. The phylogenetic tree using env gene sequence showed strains Ibaraki, No.43 and Kagoshima were sorted in the same cluster including the USA and Chinese strains, while Hokkaido strain was in the other cluster including European strains. Although no clear correlation between BFV and BLV could be found, BFV and BLV infections were likely to increase with ages. Our data on epidemiology and characteristics of BFV will provide important information to reveal biological features of BFV. 相似文献
20.
Abdul Razaq IRSHAD Taihei SASAKI Tomoaki KUBO Naoyuki ODASHIMA Keiji KATANO Takeshi OSAWA Toru TAKAHASHI Yoshiaki IZAIKE 《The Journal of reproduction and development》2015,61(5):485-488
The objectives of the present study were to develop a programmable piggyback syringe pump for bovine superovulation and to evaluate the effects of a four-times-a-day injection regimen using the pump. Non-lactating Holstein cows were treated with a total of 30 armour units of porcine FSH by injection four times a day with the pump (study, n = 9) or injection twice a day manually (control, n = 9) for four consecutive days from D10 of the estrous cycle. The pump-driven program successfully induced superovulation in all cows tested. The numbers of small (3– < 5 mm in diameter) and large (≥ 10 mm in diameter) follicles were greater in the study group on D11-13 and D14, respectively. There were fewer unovulated follicles detected on D21 (7 days after estrus) in the study group than in the control group (1.2 ± 0.4 and 3.2 ± 0.6, respectively). 相似文献