Natural infection of gastrointestinal nematodes in long-nosed armadillos Dasypus novemcinctus Linnaeus, 1758 from Pantanal wetlands, Aquidauana sub-region, Mato Grosso do Sul State, with the description of Hadrostrongylus speciosum n. gen. et n. sp. (Molineidae: Anoplostrongylinae)     

Lux Hoppe EG  do Nascimento AA 《Veterinary parasitology》2007,144(1-2):87-92

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Evidence of an attractant from virgin females of Bephratelloides pomorum (Hymenoptera: Eurytomidae): possible role of cuticular compounds     

Ruth R. do Nascimento  Ivanildo S. de Lima  Cludia M. L. de Mendona  Luciano C. N. da Silva  Adriana G. Duarte  Antnio E. G. Sant'Ana 《Pest management science》1998,54(3):311-313

The Annona seed borer, Bephratelloides pomorum (Hymenoptera: Eurytomidae), is the most important insect pest of soursop, Annona muricata L. The female lays eggs directly into the most recently formed seeds of developing fruit where the larvae remain feeding until pupation. When fully developed, the young wasps make a channel to emerge from the fruit, ready to copulate. Males are attracted to females and display a peculiar behaviour which consists of three steps: antennation, lateral bouncing and wing vibrations. Experiments carried out in semi-field and laboratory conditions showed that males also behave similarly in the presence of filter paper impregnated with a hexane extract of the female's body, suggesting that female B. pomorum produce an attractant which enables males to find them. The hexane extracts of females, analysed by GC-MS, were shown to consist mainly of a mixture of straight- and branched-chain hydrocarbons and esters. © 1998 Society of Chemical Industry  相似文献   

Chemical, Sensorial and Rheological Properties of a New Organic Rice Bran Beverage     

Letícia Adélia MIOTTo 《水稻科学》2009,16(3):226-234

Rice bran is a solid residue from rice polishing that is used in animal nutrition and rice oil production. Cultivation conditions with agro-toxics, lipids instability, and tendency for mycotoxin contamination restrict its application in human nutrition. Therefore, organic agriculture is an alternative to use the properties of rice bran. Rice bran beverage is a new cereal product from organic rice. This work presents the preliminary results of the chemical and rheological studies of a bath pasteurized rice bran beverage. Compared with integral defatted milk, soy extracts, and brown rice low-fat milk, the rice bran beverage studied in this work presents itself as an important source of minerals and unsaturated lipids. All essential amino acids were found in this product. Glutamic and aspartic acids were predominant. Bath pasteurization at boiling water temperature for 15 and 30 min was adequate for microbiological safety. Refrigeration storage for 20 days, evaluated by pH and acidity variations, was ideal for assessment of the beverage conservation time. The beverage viscosity was of the Newtonian standard behavior, and its viscosity during storage was not a good parameter to evaluate shelf life. Sensory preference tests showed positive perspectives for this new beverage.  相似文献   

Amino acid digestibility of animal protein ingredients for bullfrogs in different phases of post‐metamorphic development          下载免费PDF全文

Cleber Fernando Menegasso Mansano  Beatrice Ingrid Macente  Thiago Matias Torres Nascimento  Marcelo Maia Pereira  Leonardo Sussumu Takahashi  Marta Verardino De Stéfani 《Aquaculture Research》2017,48(9):4822-4835

The development of diets that economically and ecologically meet the requirements of bullfrogs (Lithobates catesbeianus) is important for frog farming. Therefore, the objective of this study was to determine the apparent digestibility coefficients for essential amino acids of eight animal feed ingredients (poultry byproduct meal, meat and bone meal, salmon byproduct meal, tilapia byproduct meal, spray‐dried blood meal, powered red blood cells, feather meal and sardine meal) used in the diets of bullfrogs. Additionally, the limiting digestible amino acids were determined for each ingredient. Bullfrogs in different phases of post‐metamorphic development were used: 810 animals in the early phase (mean weight: 40 ± 10 g), 405 animals in the growing phase (95 ± 15 g) and 243 animals in the finishing phase (175 ± 25 g). Poultry byproduct meal, salmon byproduct meal and blood meal exhibited the largest number of high apparent digestibility coefficients for essential amino acids (>70%) in the early, growing and finishing phases, respectively. The animal protein ingredients were well digested by the bullfrogs and could be used in practical diets for this species, respecting the limits of inclusion and digestibility values for each developmental phase.  相似文献   

Long-term agroecosystem experiments: assessing agricultural sustainability and global change   总被引：3，自引：0，他引：3  

PE Rasmussen  KWT Goulding  JR Brown  PR Grace  HH Janzen  M Korschens 《Science (New York, N.Y.)》1998,282(5390):893-896

Long-term agroecosystem experiments can be defined as large-scale field experiments more than 20 years old that study crop production, nutrient cycling, and environmental impacts of agriculture. They provide a resource for evaluating biological, biogeochemical, and environmental dimensions of agricultural sustainability; for predicting future global changes; and for validating model competence and performance. A systematic assessment is needed to determine the merits of all known experiments and to identify any that may exist in tropical and subtropical environments. The establishment of an international network to coordinate data collection and link sites would facilitate more precise prediction of agroecosystem sustainability and future global change.  相似文献   

Magnetic microstructure of magnetotactic bacteria by electron holography     

RE Dunin-Borkowski  MR McCartney  RB Frankel  DA Bazylinski  M Posfai  PR Buseck 《Science (New York, N.Y.)》1998,282(5395):1868-1870

Off-axis electron holography in the transmission electron microscope was used to correlate the physical and magnetic microstructure of magnetite nanocrystals in magnetotactic bacteria. The magnetite crystals were all single magnetic domains, and the magnetization directions of small superparamagnetic crystals were constrained by magnetic interactions with larger crystals in the chains. Shape anisotropy was found to dominate magnetocrystalline anisotropy in elongated crystals. A coercive field between 300 and 450 oersted was determined for one chain.  相似文献   

Molecular cloning and characterization of an alpha-amylase occurring in the pulp of ripening bananas and its expression in Pichia pastoris     

Junior AV  do Nascimento JR  Lajolo FM 《Journal of agricultural and food chemistry》2006,54(21):8222-8228

Alpha-amylases (EC 3.2.1.1) are glycosyl hydrolases with endoglycolytic activity on the alpha-1,4-d-glucosidic linkages in starch. In bananas, the mobilization of starch accounts for sugar accumulation during ripening, and among several hydrolytic enzymes, alpha-amylase is the only enzyme argued to be able to attack the intact granules, indicating a pivotal role for this enzyme. A 1953 bp full-length banana alpha-amylase cDNA (MAmy), encoded for a sequence of 416 amino acids, was cloned and used for heterologous expression in Pichia pastoris. The cloned MAmy presented the highly conserved motifs common to alpha-amylases, and the amylolytic activity of the extracts from yeast transformed with MAmy demonstrated that it encodes for a functional alpha-amylase, suggesting a putative role for this gene in starch degradation during fruit ripening.  相似文献   

Testicular alterations in cryptorchid/orchiopexic rats chronically exposed to acrylamide or di-butyl-phthalate     

Thania R. R. Lima  Nathlia P. Souza  Ana P. Ferragut Cardoso  Lígia M. M. Gomide  Merielen G. Nascimento e Pontes  Hlio A. Miot  Lora L. Arnold  Samuel M. Cohen  Joo Lauro V. de Camargo 《Journal of toxicologic pathology》2022,35(2):159

Exposure of Sprague-Dawley (SD) rats to acrylamide (AA) or di-butyl-phthalate (DBP) from the 12th gestational day to the 16th postnatal week (PNW) has been shown to reduce the effectiveness of orchiopexy in recovering the testicular alterations associated with experimental cryptorchidism established at weaning. Herein, we provide information about the long-term effects of AA or DBP on the testes of cryptorchid/orchiopexic rats. Male offspring exposed in utero to 10 mg/kg/day AA or 500 mg/kg/day DBP underwent bilateral surgical cryptorchidism at the 3rd PNW and orchiopexy at the 6th week, with continuous exposure to the chemicals through diet until the 58th week. Regardless of the test chemical, there were severe qualitative/quantitative alterations in the seminiferous tubules and increased numbers of Leydig cells. There was an increase and decrease in the number of tubules with c-Kit- and placental alkaline phosphatase-labeled germ cells, respectively, as compared to those in the control group, suggesting an imbalance between apoptosis and cell proliferation processes. The histological scores of the testicular lesions at the end of this one-year study were higher than those in the previous 16-week study, indicating that exposure of rats to the toxicants AA or DBP enhanced the testicular alterations induced by the chemicals beginning at the intra-uterine life, and impaired the effectiveness of orchiopexy in restoring the testes to normal morphology. Although the present experimental protocol does not completely replicate the natural human undescended testes, our findings may contribute to understanding the alterations occurring in cryptorchid/orchiopexic testes potentially exposed to exogenous chemicals for extended periods.  相似文献   

Copper-Catalyzed Oxidation of Alcohols to Aldehydes and Ketones: An Efficient, Aerobic Alternative   总被引：1，自引：0，他引：1  

IE Marko  PR Giles  M Tsukazaki  SM Brown  CJ Urch 《Science (New York, N.Y.)》1996,274(5295):2044-2046

An efficient, copper-based catalyst has been discovered that oxidizes a wide range of alcohols into aldehydes and ketones under mild conditions. This catalytic system utilizes oxygen or air as the ultimate, stoichiometric oxidant, producing water as the only by-product.  相似文献   

Survival of Curtobacterium flaccumfaciens pv. flaccumfaciens in weeds     

Daniele M. Nascimento  Letícia R. Oliveira  Luana L. Melo  João C. Silva  José M. Soman  Karine T. Girotto  Renan P. Eburneo  Marcos R. Ribeiro-Junior  Maria M. P. Sartori  Tadeu A. F. Silva Júnior  Antonio C. Maringoni 《Plant pathology》2020,69(7):1357-1367

Weeds are important alternative hosts of pathogens, responsible for the survival and spread of phytopathogenic bacteria. Our study evaluated the potential of weeds as hosts of Curtobacterium flaccumfaciens pv. flaccumfaciens (Cff), causal agent of bacterial wilt, one of the main diseases of common beans. Cff survival was evaluated in the phyllosphere and in the rhizosphere of 21 weeds, in four experiments under field conditions, during the years 2018 and 2019. The aerial part of the plant was inoculated by spraying bacterial suspension (10⁷ cfu/ml) of Cff, while the soil of the growing pots was infested with the same suspension. Cff survival was evaluated every 7 days, for 70 days. The identity of the bacterium was confirmed by PCR with the specific primers CffFOR2 and CffREV4, from strains recovered from all samples. Principal component analysis (PCA) showed that high temperatures and rainfall reduced Cff survival in the phyllosphere, while high temperatures reduced the survival of the bacterium in the rhizosphere. Our results demonstrated that Amaranthus viridis (family Amaranthaceae), Conyza bonariensis, Emilia fosbergii, Galinsoga parviflora, Gnaphalium purpureum (Asteraceae), Raphanus sativus, Lepidium virginicum (Brassicaceae), Commelina benghalensis (Commelinaceae), Ipomoea triloba (Convolvulaceae), Cyperus rotundus (Cyperaceae), Senna obtusifolia (Fabaceae), Digitaria insularis (Poaceae), Nicandra physalodes, and Solanum americanum (Solanaceae) are potential hosts for Cff. Their eradication in common bean fields is recommended, especially in fields with a history of bacterial wilt occurrence.  相似文献