Gross composition,fatty acid profile and sensory characteristics of Saanen goat milk fed with Cacti varieties     

Karen Luanna Marinho Catunda  Emerson Moreira de Aguiar  Pedro Etelvino de Góes Neto  José Geraldo Medeiros da Silva  José Aparecido Moreira  Adriano Henrique do Nascimento Rangel  Dorgival Morais de Lima Júnior 《Tropical animal health and production》2016,48(6):1253-1259

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Transcriptomic investigation of the molecular mechanisms underlying resistance to the neonicotinoid thiamethoxam and the pyrethroid lambda-cyhalothrin in Euschistus heros (Hemiptera: Pentatomidae)     

Ewerton C Lira  Antonio RB Nascimento  Chris Bass  Celso Omoto  Fernando L Cônsoli 《Pest management science》2023,79(12):5349-5361

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Diagnosis of canine brucellosis by ELISA using an antigen obtained from wild Brucella canis     

Barrouin-Melo SM  Poester FP  Ribeiro MB  de Alcântara AC  Aguiar PH  Nascimento IL  Schaer RE  Nascimento RM  Freire SM 《Research in veterinary science》2007,83(3):340-346

An indirect ELISA test was developed for the diagnosis of Brucella canis infection in dogs. A bacterial whole cell extract was used as a solid phase antigen, using B. canis isolated from an infected animal. Sera from culture-positive and healthy negative animals were used as internal reference controls. The cut-off point was determined by a mathematical formula for a statistically valid value, which defined the upper prediction limit, based on the upper tail of the t-distribution of 21 negative control sera readings, for the confidence level of 99.5%. The sensitivity and specificity of the ELISA test were 95% and 91%, respectively. The ELISA test showed a significant concordance index (K=0.84) with the agar gel immunodiffusion test. The reliability of the ELISA for the detection of infected animals was established by a double blind study testing 280 sera provided by serum banks from different diagnostic and research institutions and analyzed by ROC Curve.  相似文献   

Coastal taipan (Oxyuranus scutellatus) envenomation of a dog          下载免费PDF全文

PR Judge 《Australian veterinary journal》2015,93(11):412-416

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Characterization of Isoforms of the Lectin Isolated from the Red Algae Bryothamnion seaforthii and Its Pro-Healing Effect     

Luiz Gonzaga do Nascimento-Neto  Romulo Farias Carneiro  Suzete Roberta da Silva  Bruno Rocha da Silva  Francisco Vassiliepe Sousa Arruda  Victor Alves Carneiro  Kyria Santiago do Nascimento  Silvana Saker-Sampaio  Valdemiro Amaro da Silva Jr.  Ana L��cia Figueiredo Porto  Benildo Sousa Cavada  Alexandre Holanda Sampaio  Edson Holanda Teixeira  Celso Shiniti Nagano 《Marine drugs》2012,10(9):1936-1954

Lectins are a structurally heterogeneous group of proteins that have specific binding sites for carbohydrates and glycoconjugates. Because of their biotechnological potential, lectins are widely used in biomedical research. The present study aimed to evaluate the healing potential of the lectin isolated from the marine red alga Bryothamnion seaforthii (BSL). The lectin was purified using ion exchange chromatography with DEAE cellulose and characterized using tandem mass spectrometry. For healing tests, skin wounds were induced in the dorsal thoracic region of mice. These animals were randomly divided into three groups and subjected to topical treatment for 12 days with BSL, bovine serum albumin and 150 mM NaCl. To evaluate the potential of each treatment, the animals were anesthetized and sacrificed on days 2, 7 and 12, respectively. The parameters evaluated included the wound area, the proportion of wound closure and the histological diagnosis. The wound closure was more effective with BSL (Postoperative Day 7 and 12) than controls. The luminal epithelium was completely restructured; the presence of collagen in the dermis and the strongly active presence of young skin annexes demonstrate the potential of treatment with BSL compared with controls. Our findings suggest that BSL has pro-healing properties and can be a potential medical process in the treatment of acute wounds.  相似文献   

The effect of silicon on antioxidant metabolism of wheat leaves infected by Pyricularia oryzae     

D. Debona  F. A. Rodrigues  J. A. Rios  K. J. T. Nascimento  L. C. Silva 《Plant pathology》2014,63(3):581-589

This study investigated whether the increase in wheat resistance to blast, caused by Pyricularia oryzae, potentiated by silicon (Si) is linked to changes in the activity of antioxidative enzymes. Wheat plants (cv. BR 18) were grown in hydroponic culture with either 0 (–Si) or 2 mm (+Si) Si and half of the plants in each group were inoculated with P. oryzae. Blast severity in the +Si plants was 70% lower compared to the ?Si plants at 96 h after inoculation (hai). Superoxide dismutase (SOD), catalase (CAT), peroxidase (POX), ascorbate peroxidase (APX) and glutathione‐S‐transferase (GST) activities were higher in the leaves of the ?Si plants compared with the +Si plants at 96 hai. This indicates that other mechanisms may have limited P. oryzae infection in the +Si plants restricting the generation of reactive oxygen species, obviating the need for increased antioxidative enzyme activity. In contrast, glutathione reductase (GR) activity at 96 hai was higher in the +Si plants than in the ?Si plants. Although the inoculated plants showed significantly higher concentration of malondialdehyde (MDA) than the non‐inoculated plants, lower MDA concentrations were observed in the +Si plants compared with the ?Si plants. The lower MDA concentration associated with decreased activities of SOD, CAT, POX, APX and GST, suggest that the amount of reactive oxygen species was lower in the +Si plants. However, GR appears to play a pivotal role in limiting oxidative stress caused by P. oryzae infection in +Si plants.  相似文献   

Digestibility of nutrients and energy in ingredients for bullfrogs during different phases of development          下载免费PDF全文

C.F.M. Mansano  B.I. Macente  T.M.T. Nascimento  D.F.H. Pinto  M.M. Pereira  M.V. De Stéfani 《Aquaculture Nutrition》2017,23(6):1368-1378

The ability of frogs to digest dietary nutrients changes with growth, with the animals becoming more or less demanding. The objective of this study was to determine the apparent digestibility coefficients of protein, energy and ether extract of 14 ingredients used in bullfrog diets (spray‐dried blood meal, hydrolysed feather meal, poultry by‐product meal, red blood cell concentrate, tilapia by‐product meal, sardine fishmeal, salmon fishmeal, meat and bone meal, corn gluten meal, soybean meal, corn, wheat bran, soy protein concentrate and soybean oil). A total of 2,325 bullfrogs (Lithobates catesbeianus) in different phases of development were used: early phase (mean weight of 30–50 g), growth phase (80–110 g) and finishing phase (150–200 g). Faeces were collected using the method of dissection. Marked differences in digestibility of the ingredients were observed between the different phases of frog development. Among the ingredients studied, salmon fishmeal and corn gluten meal showed good utilization of the protein (78.9% and 86.7%, respectively) and energy fraction (89.4% and 83.3%, respectively). The salmon fishmeal, poultry by‐product meal, sardine fishmeal, soy protein concentrate, wheat bran and soybean oil exhibited good ether extract utilization (81.2%–92.8%), recommending their use in bullfrog diets.  相似文献   

Effect of broodstock age on reproductive parameters in silver catfish Rhamdia quelen          下载免费PDF全文

Cleonice Cristina Hilbig  Nivaldo Ferreira do Nascimento  Marcos Granville  Cesar Rebechi de Toledo  Giovano Neumann  Robie Allan Bombardelli  Laura Satiko Okada Nakaghi 《Aquaculture Research》2017,48(9):5150-5154

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Influence of magnesium on physiological responses of wheat infected by Pyricularia oryzae          下载免费PDF全文

D. Debona  J. A. Rios  K. J. T. Nascimento  L. C. Silva  F. A. Rodrigues 《Plant pathology》2016,65(1):114-123

Although magnesium (Mg) is considered an essential element for wheat growth, its importance for disease control has often been overlooked, and the physiological features of diseased plants mediated by Mg remain elusive. In this study, the effect of three Mg concentrations (0·25, 2·5 and 4 mm ) on wheat resistance to leaf blast (Pyricularia oryzae), leaf gas exchange, invertase activity, cellular damage and foliar concentration of photosynthetic pigments and nutrients was investigated. Foliar Mg increased from 1·9 to 3·9 g kg^?1, whereas calcium (Ca) decreased from 7·8 to 4·9 g kg^?1 as the applied Mg increased from 0·25 to 4 mm . Blast severity increased from 11·3 to 39·6% as the applied Mg increased from 0·25 to 4 mm . Photosynthesis, stomatal conductance, transpiration and photosynthetic pigment concentrations decreased in inoculated plants compared to non‐inoculated plants regardless of the Mg concentration; however, the reductions were more pronounced for plants grown with 4 mm Mg than those grown with 0·25 mm Mg. On the other hand, a higher internal CO₂ concentration, invertase activity and malondialdehyde concentration was recorded in inoculated plants grown with 4 mm Mg compared to those grown with 0·25 mm Mg. In conclusion, reduced Ca uptake may partially explain the increased susceptibility of wheat to leaf blast with the highest Mg concentration. Mg‐induced susceptibility to leaf blast appeared responsible for the photosynthetic impairments. These were most probably due to biochemical constraints because plants grown with the highest Mg concentration suffered extensive cellular damage and degradation of photosynthetic pigments as a result of high disease severity.  相似文献   

Alternative control of Chalara paradoxa,causal agent of black rot of pineapple by plant extract of Mormodica charantia     

de Souza  Wilza Carla Oliveira  do Nascimento  Luciana Cordeiro  Vieira  Daniele Lavra  dos Santos  Taciana Silva  de Assis Filho  Francisco Miguel 《European journal of plant pathology / European Foundation for Plant Pathology》2015,142(3):481-488

European Journal of Plant Pathology - Black rot of pineapple caused by the fungus Chalara paradoxa is considered an aggressive and difficult disease to control. The use of natural products has been...  相似文献