Genetic basis of Spodoptera frugiperda (Lepidoptera: Noctuidae) resistance to the chitin synthesis inhibitor lufenuron          下载免费PDF全文

Antonio Rogério Bezerra do Nascimento  Juliano Ricardo Farias  Daniel Bernardi  Renato Jun Horikoshi  Celso Omoto 《Pest management science》2016,72(4):810-815

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Filamentous Fungi Isolates of Contaminated Sediment in the Amazon Region with the Potential for Benzo(a)pyrene Degradation     

Hilton Marcelo de Lima Souza  Lara Durães Sette  Adolfo José da Mota  Joaquim Ferreira do Nascimento Neto  André Rodrigues  Tássio Brito de Oliveira  Fernando Mendes de Oliveira  Luiz Antônio de Oliveira  Hileia dos Santos Barroso  Sandra Patricia Zanotto 《Water, air, and soil pollution》2016,227(12):431

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Silicon Effects on Metal Tolerance and Structural Changes in Maize (Zea mays L.) Grown on a Cadmium and Zinc Enriched Soil     

da Cunha  Karina Patrícia Vieira  do Nascimento  Clístenes Williams Araújo 《Water, air, and soil pollution》2009,199(1-4):323-341

Water, Air, & Soil Pollution - The southern sector of the Guadiana River basin (GRB) drains the central-western part of the Iberian Pyrite Belt, an area with many polymetallic sulfide deposits...  相似文献   

Parasitological diagnosis of canine visceral leishmaniasis: Is intact skin a good target?     

M.F. Madeira  F.B. Figueiredo  A.G.S. Pinto  L.D. Nascimento  M. Furtado  E. Mouta-Confort  C.C. de Paula  A. Bogio  M.C.A. Gomes  A.M.S. Bessa  S.R.L. Passos 《Research in veterinary science》2009,87(2):260-262

The objective of this study was to evaluate intact skin of seroreactive dogs as a possible target for the parasitological confirmation of canine visceral leishmaniasis (CVL). For this purpose, 394 dogs identified in serological surveys carried out in the metropolitan region of Belo Horizonte were studied. Blood was collected from all animals for serology and a tissue sample was obtained from two sites for parasitological diagnosis. Skin obtained from the ear and scapular region was simultaneously analyzed in 247 animals and lesion samples and ear skin were analyzed in 147 dogs. Leishmania parasites were isolated from 310 (78.7%) animals, and all isolates were identified as Leishmania chagasi. Simultaneous isolation from two sites was possible in 240 of the 310 animals, including ear and scapular skin in 151/247 (61.1%) and ear skin and skin lesions in 89/147 (60.5%). Ours results suggest that intact skin is one of the main target sites for the parasitological confirmation of CVL in seroreactive dogs.  相似文献   

Montenegro's skin reactions and antibodies against different Leishmania species in dogs from a visceral leishmaniosis endemic area     

Baleeiro CO  Paranhos-Silva M  dos Santos JC  Oliveira GG  Nascimento EG  de Carvalho LP  dos-Santos WL 《Veterinary parasitology》2006,139(1-3):21-28

In this study, humoral (circulating anti-Leishmania antibodies) and cellular (Montenegro's skin test) immune responses of dogs from an endemic area of visceral leishmaniosis were tested using Leishmania chagasi, Leishmania amazonensis and Leishmania braziliensis antigens. The antibody response was tested in three animal groups, selected according to their anti-L. chagasi antibody activity, as measured by ELISA in the serum: 19 negative (O.D. below 0.30), seven with undefined (O.D. between 0.40 and 0.70) and 12 positive (O.D. above 1.0) ELISA result. In the group of animals with positive ELISA, the antibody activity against L. chagasi antigens (mean O.D.=1.31) was significantly higher (ANOVA, P<0.01) than against L. amazonensis (mean O.D.=0.88) or L. braziliensis (mean O.D.=0.87) antigens. The Montenegro's skin test results obtained with L. chagasi and L. braziliensis antigens showed a fair agreement (kappa=0.309). The same was observed when antigens from L. braziliensis and L. amazonensis were compared (kappa=0.374), whereas a moderate agreement between the results from tests performed with L. chagasi and L. amazonensis antigens was observed (kappa=0.530). The induration areas obtained with L. braziliensis antigen were smaller than those obtained with the other antigens. The data presented herein indicate that the use of antigens from different Leishmania species may interfere with the results of the immunological tests performed in dogs in an endemic area of visceral leishmaniosis.  相似文献   

Path analysis for selection of drought tolerant sugarcane genotypes through physiological components     

Paulo Pedro da Silva  Lailton SoaresJoão Gomes da Costa  Luciana da Silva VianaJúlio César Farias de Andrade  Eduardo Rebelo GonçalvesJoão Messias dos Santos  Geraldo Veríssimo de Souza BarbosaVelber Xavier Nascimento  Adriana Reis TodaroAlessandro Riffel  Maria Fatima Grossi-de-SaMárcio Henrique Pereira Barbosa  Antônio Euzébio Goulart Sant’AnaCícero Eduardo Ramalho Neto 《Industrial Crops and Products》2012,37(1):11-19

Water deficit is among the main environmental factors limiting agricultural productivity of sugarcane in Northeast Brazil by affecting virtually every aspect of plant growth, with consequent reduction of the agro-industrial productivity of this crop. This study aimed to use path analysis to evaluate the physiological components of sugarcane under two water conditions, with photosynthesis as the basic variable, in order to obtain subsidies that can help the genetic breeding of this crop by selection of superior clones with drought tolerance. The experiment was carried out in a greenhouse with a completely randomized experimental design, 4 × 2 factorial arrangements (four sugarcane genotypes × two soil moisture regimes: control, with 80-100% available water and stressed, with 0-20% water available) and with four replications. Each plot consisted of one pot with 12 kg of substrate containing one plant. Treatments were maintained at a humidity close to field capacity with daily replacement of the evapotranspired water with a water supply for 66 days of cultivation for treatments under stress. Evaluations were conducted on the fourth day after severe water stress at 70 days of cultivation. Data from nine physiological variables were considered, with six of these being used in the model after exclusion of those that were contributing to multicollinearity. Variables used in path analysis were enough to explain the variation found in photosynthesis under both water conditions. Stomatal conductance, transpiration and the SPAD index should be considered as a priority in breeding programs for sugarcane aiming to get more productive and tolerant genotypes to water stress, and selection indexes should be used with due consideration of these variables to promote gains in photosynthesis.  相似文献   

Release of doxycycline through cellulose acetate symmetric and asymmetric membranes produced from recycled agroindustrial residue: Sugarcane bagasse     

Guimes Rodrigues Filho  Sabrina Dias RibeiroCarla da Silva Meireles  Leandro Gustavo da SilvaReinaldo Ruggiero  Moacir Fernandes Ferreira JuniorDaniel Alves Cerqueira  Rosana Maria Nascimento de AssunçãoMara Zeni  Patricia Polleto 《Industrial Crops and Products》2011,33(3):566-571

Cellulose acetate is one of the components employed in drug controlled-release systems in the form of membranes. The aim of this study was to examine the controlled-release of doxycycline employing cellulose acetate symmetric and asymmetric membranes as matrices. The cellulose triacetate was produced from sugarcane bagasse through a homogeneous acetylation reaction, using acetic acid as the solvent, acetic anhydride as the acetylating agent and sulfuric acid as the catalyst. The viscosity average molecular weight of the cellulose acetate produced was 39,000 g mol⁻¹. The symmetric membranes were produced using a system solvent of dichloromethane/ethanol (9:1, v/v) and the asymmetric membranes were produced from the same solvent system and 10% of water. For the formulation of both, 5% of doxycycline was used. The membranes were characterized by thermal analysis (DSC and TGA) and scanning electron microscopy SEM. The release of doxycycline through cellulose triacetate matrices was examined using spectrophotometric analysis in the ultraviolet-visible region, at 275 nm. The results revealed that asymmetric membranes release 80% of the drug in 100 min, while symmetric membranes release 14% of the drug during the same time interval.  相似文献   

Sites of Persistence of Lumpy Skin Disease Virus in the Genital Tract of Experimentally Infected Bulls     

CH Annandale  PC Irons  VP Bagla  UI Osuagwuh  EH Venter 《Reproduction in domestic animals》2010,45(2):250-255

The objectives of this work were to determine the site of persistence of lumpy skin disease virus (LSDV) in bulls shedding the virus in semen for a period longer than 28 days, to determine if the virus is present in all fractions of semen and to study lesions that developed in the genital tract. Six serologically negative postpubertal bulls were experimentally infected with a virulent field isolate of LSDV. The polymerase chain reaction (PCR) was performed on sheath washes, vesicular fluid, supernatant and cell‐rich fractions of semen from day 10 to day 26 postinfection (p.i.). Bulls that were positive by PCR on the whole semen sample collected on day 28 p.i. were slaughtered and tissue samples from their genital tracts submitted for histopathological evaluation, immunoperoxidase staining, virus isolation and PCR. Two of the bulls developed severe lumpy skin disease (LSD) and were found to be shedding viral DNA in their semen on day 28 p.i. Viral DNA was identified in all semen fractions from all bulls, but mostly from the cell‐rich fraction and from the severely affected bulls. The PCR assay was positive on postmortem samples of testes and epididymides from the two severely affected bulls. Virus could be recovered from the testes of these two bulls and from the epididymis of one of them. Immunoperoxidase staining was positive for LSDV staining in sections of testes and epididymides exhibiting necrosis. This study suggests that the testis and epididymis are sites of persistence of LSDV in bulls shedding virus in semen for prolonged periods and revealed that viral DNA is present in all fractions of the ejaculate.  相似文献   

Differential display and suppression subtractive hybridization analysis of the pulp of ripening banana     

Adriana de Godoy  Beatriz Rosana CordenunsiFranco Maria Lajolo  João Roberto Oliveira do Nascimento 《Scientia Horticulturae》2010

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Effects of dredging operations on sediment quality: contaminant mobilization in dredged sediments from the Port of Santos, SP, Brazil     

Ronaldo J. Torres  Denis M. S. Abessa  Fernando C. Santos  Luciane A. Maranho  Marcela B. Davanso  Marcos R. L. do Nascimento  Antonio A. Mozeto 《Journal of Soils and Sediments》2009,9(5):420-432

Background, aim, and scope  

Contaminated sediments are a worldwide problem, and mobilization of contaminants is one of the most critical issues in environmental risk assessment insofar as dredging projects are concerned. The investigation of how toxic compounds are mobilized during dredging operations in the channel of the Port of Santos, Brazil, was conducted in an attempt to assess changes in the bioavailability and toxicity of these contaminants.  相似文献