Immunogenicity in dogs of three recombinant antigens (TSA, LeIF and LmSTI1) potential vaccine candidates for canine visceral leishmaniasis     

Fujiwara RT  Vale AM  França da Silva JC  da Costa RT  Quetz Jda S  Martins Filho OA  Reis AB  Corrêa Oliveira R  Machado-Coelho GL  Bueno LL  Bethony JM  Frank G  Nascimento E  Genaro O  Mayrink W  Reed S  Campos-Neto A 《Veterinary research》2005,36(5-6):827-838

Control of canine visceral leishmaniasis (VL) remains a difficult and serious problem mostly because there is no reliable and effective vaccine available to prevent this disease. A mixture of three recombinant leishmanial antigens (TSA, LeIF and LmSTI1) encoded by three genes highly conserved in the Leishmania genus have been shown to induce excellent protection against infection in both murine and simian models of cutaneous leishmaniasis. A human clinical trial with these antigens is currently underway. Because of the high degree of conservation, these antigens might be useful vaccine candidates for VL as well. In the present study, using the dog model of the visceral disease, we evaluated the immunogenicity of these three antigens formulated with two different adjuvants, MPL-SE and AdjuPrime. The results were compared with a whole parasite vaccine formulated with BCG as the adjuvant. In order to investigate if sensitization with the recombinant antigens would result in recognition of the corresponding native parasite antigens upon infection, the animals were exposed for four weeks after the termination of the immunization protocol with the recombinant antigens to a low number of L. chagasi promastigotes, an etiological agent of VL. Immune response was evaluated by quantitative ELISA in the animal sera before and after exposure to the viable parasites. Both antigen specific IgG1 and IgG2 antibody levels were measured. Immunization of dogs with the recombinant antigens formulated in either MPL-SE or AdjuPrime resulted in high antibody levels particularly to LmSTI1. In addition, this immunization although to low levels, resulted in the development of antibody response to the whole parasite lysate. Importantly, experimental exposure with low numbers of culture forms of L. chagasi promastigotes caused a clear boost in the immune response to both the recombinant antigens and the corresponding native molecules. The boost response was predominantly of the IgG2 isotype in animals primed with the recombinant antigens plus MPL-SE. In contrast, animals primed with the recombinant antigens formulated in AdjuPrime as well as animals vaccinated with crude antigen preparation responded with mixed IgG1/IgG2 isotypes. These results point to the possible use of this antigen cocktail formulated with the adjuvant MPL-SE in efficacy field trials against canine VL.  相似文献   

Pulsatile Secretory Pattern of Gonadotrophins and Ovarian Steroids during the Periovulatory Phase of the Oestrous Cycle in the Buffalo     

Baljit Singh  VD Dixit  VP Dixit  IS Lohan  GC Georgie 《Reproduction in domestic animals》1998,33(5):363-366

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Effects of Sperm Concentration and Straw Volume on Motion Characteristics and Plasma, Acrosomal, and Mitochondrial Membranes of Equine Cryopreserved Spermatozoa     

J. Nascimento DVM  MS  C.F. Raphael DVM  MS  A.F.C. Andrade DVM  M.A. Alonso DVM  MS  E.C.C. Celeghini DVM  MS  PhD  R.P. Arruda DVM  MS  PhD   《Journal of Equine Veterinary Science》2008,28(6):351-358

The advantages of using cryopreserved semen in equine reproduction are well known. During cryopreservation, spermatozoa undergo many changes that lead to a decrease in fertility. There is no agreement on the ideal sperm dose and concentration to maximize fertility rates. Thus, the objectives of this experiment were to evaluate sperm motion by computer-assisted analysis (CASA), sperm membrane integrity and function with fluorescence probes of cryopreserved sperm at three concentrations: 100 (C100), 200 (C200) and 400 × 10⁶ sperm/mL (C400), and two straw volumes (0.50 and 0.25 mL). There was no interaction between sperm concentration and storage volume (P > .05). Sperm motion characteristics were influenced by concentration (C100 > C200 > C400; P < .05). Curvilinear velocity (VCL) in 0.25-mL straws had higher average values (P < .05). Membrane integrity and function were not changed by straw volume (P > .05). However, sperm concentration changed the percentage of cells with intact plasma membrane (C100 > C200 > C400; P < .05) and the percentage of cells with high mitochondrial membrane potential (C100 = C200; P > .05 and C400 < C100 and C200; P < .05). According to this experiment, the best freezing method was that involving 100 × 10⁶ sperm/mL, regardless of straw volume.  相似文献   

Differentiation between canine cutaneous and visceral leishmaniasis by the detection of immunoglobulin G specific for Leishmania (Viannia) braziliensis and Leishmania (Leishmania) chagasi antigens using flow cytometry     

Santiago Mde A  Ribeiro FC  Mouta-Confort E  Nascimento LD  Schubach Ade O  Madeira Mde F  Bertho AL 《Veterinary parasitology》2008,154(3-4):341-346

Flow cytometry employing Leishmania (L.) chagasi (Lc) and L. (Viannia) braziliensis (Lb) antigen was used to establish the differential diagnosis between visceral (VL) and cutaneous leishmaniasis (CL) in dogs. Flow cytometry permitted the detection of Leishmania-specific immunoglobulin G in sera from 19 dogs: nine with CL and 10 with VL. A significant difference in the percentage of positive staining was observed in sera from dogs with CL between the homologous antigen (69% for Lb) and the heterologous antigen (42% for Lc). However, this difference was not significant in sera from dogs with VL (61% for Lb and 73% for Lc). No significant staining was observed in control sera (0.6% for Lb and 0.4% for Lc) consisting of samples from healthy dogs, or in the group with sporotrichosis (1.8% for Lb and 1.5% for Lc), a differential diagnosis of CL. The results suggest that flow cytometry might be useful for the differentiation between CL and VL in dogs, with practical applications in areas where the two infections overlap.  相似文献   

A clinical trial of a topical preparation of miconazole, polymyxin and prednisolone in the treatment of otitis externa in dogs   总被引：3，自引：0，他引：3  

VP STUDDERT  KL HUGHES 《Australian veterinary journal》1991,68(6):193-195

A topical preparation containing miconazole, polymyxin and prednisolone was shown to be more effective in the treatment of otitis externa in 167 dogs than 2 other ear preparations containing antibiotics, an antimycotic and a corticosteroid. With miconazole, polymyxin and prednisolone, the recurrence rate was 26.7% compared with 72.6% and 54.3% when the other products were used. The mean duration of treatment required to achieve resolution of clinical signs was 9.6 days, compared with 12.2 days and 13.0 days and no cases failed to respond to treatment, compared with 17.7% and 14.3%. Malassezia canis alone (71%) or in association with bacteria (18%) was recovered from 44 of 49 ears cultured.  相似文献   

Secondary succession in a fragmented Atlantic Forest landscape: evidence of structural and diversity convergence along a chronosequence     

Ladivania Medeiros do Nascimento  Everardo Valadares de Sá Barretto Sampaio  Maria Jesus Nogueira Rodal  Ana Carolina Borges Lins-e-Silva 《Journal of Forest Research》2014,19(6):501-513

Changes in physiognomy, species composition and structure, and dispersal mechanisms of canopy and subcanopy plant assemblages were investigated along a chronosequence of three ages: 12, 20, and 50+ years old (=old-growth), three replications in each, in an Atlantic Forest landscape in Northeastern Brazil. Our objective was to investigate whether there is floristic and structural convergence along secondary succession. There were significant differences between secondary and old-growth forests in density and basal area only for the subcanopy. Differences in density between forest ages were noted when the assemblage was analyzed per diameter and height classes. Richness of canopy species of both secondary ages differed from those of old-growth forests. Some dominant species in the canopy of secondary forests showed a significant decrease in density with increasing age, which indicates an ongoing process of floristic changes. The low level of shared species between secondary and old-growth forests supports the idea that species composition is one of the last components to recover during successional process. Zoochory was the most important dispersal guild in species percentage and number, irrespective of stand age. Although regenerating areas can take alternative pathways, our results indicate that secondary Atlantic Forest sites have a high potential for natural regeneration. This recovery is recorded as a physiognomic convergence of the canopy layer in as little as 12 years, and progressive introductions of later successional species into the plant assemblage that lead to convergence in terms of the diversity and richness of the subcanopy and of dispersal guilds just 20 years after abandonment.  相似文献   

The effect of heat stress on GHR,IGF-I,ANT, UCP and COXIII mRNA expression in the liver and muscle of high and low feed efficiency female quail     

E. Gasparino  A. P. Del Vesco  D. M. Voltolini  C. S. Do Nascimento  E. Batista  A. S. Khatlab 《British poultry science》2014,55(4):466-473

1. A study was conducted to test the hypothesis that feed efficiency (FE) correlated with the expression of genes from the somatotropic axis and mitochondrial genes involved in energy production, and that the environment to which the birds are exposed influenced the expression of such genes.

2. Quails were divided into High-FE and low-FE groups and maintained in a comfortable or heat stress (HS) (38°C for 24 h) environment to evaluate changes in insulin-like growth factor I (IGF-I), growth hormone receptor (GHR), adenine nucleotide translocator (ANT), uncoupling protein (UCP) and cytochrome oxidase subunit III (COX III) messenger ribonucleic acid (mRNA) expression in liver and muscle tissues.

3. High-FE quails (0.28 g/g) presented a higher final body weight, greater weight gain and a better feed conversion ratio than low-FE birds (0.18 g/g). High-FE birds showed greater IGF-I mRNA expression in the liver and muscle and greater GHR mRNA expression in the muscle.

4. Environmental effects only affected GHR expression in the liver, with quails under comfortable conditions exhibiting greater GHR expression than quails subjected to HS.

5. There was a significant interaction between FE and environmental temperature on ANT mRNA expression in the liver. The greatest ANT mRNA expression was observed for high FE-birds that remained under comfortable conditions.

6. In the liver, UCP mRNA expression did not differ among the quails and was not affected by environment or efficiency. However, comparisons of the low- and high-FE birds revealed higher levels of UCP mRNA in the muscle of low-FE birds.

7. COX III mRNA expression in the liver was dependent on environmental temperature and FE. Higher COX III mRNA expression was observed in animals that remained under comfortable conditions, and high-FE birds exhibited higher expression levels compared to low-FE birds.

8. These results suggest a correlation between IGF-I, GHR, ANT, UCP and COX III gene expression and FE and that environmental temperature could affect the expression of some of these genes.  相似文献   

Antiproliferative effect of Canavalia brasiliensis lectin on B16F10 cells     

Flávio de Oliveira Silva  Priscila das Neves Santos  Evellyne de Oliveira Figueirôa  Cristiane Moutinho Lagos de Melo  Juliana Kelle de Andrade Lemoine Neves  Francisco Vassiliepe Sousa Arruda  João Batista Cajazeiras  Kyria Santiago do Nascimento  Edson Holanda Teixeira  Benildo Sousa Cavada  Ana Lúcia Figueiredo Porto  Valéria Rêgo Alves Pereira 《Research in veterinary science》2014

Lectins are proteins or glycoproteins with the ability to link to carbohydrates at the cell surface in a specific and reversible manner. Studies have showed that lectins are demonstrate immunomodulatory and antitumor agents. This study aims to evaluate the effect of lectin extracted from the seeds of Canavalia brasiliensis (ConBr) on murine melanoma B16F10 cells by analyzing cell viability, apoptosis index, cell migration, production of cytokines and nitric oxide (NO). Results showed that ConBr was able to reduce cell viability and thwart apoptosis, which could be observed by decrease in cell migration. ConBr also induced NO and IL-12 synthesis. Altogether, these data demonstrate the potential of ConBr as a therapeutic agent for melanoma.  相似文献   

Anti-Neospora caninum antibody detection and vertical transmission rate in pregnant zebu beef cows (Bos indicus): Neospora caninum in pregnant beef cows (Bos indicus)     

Eudes Esteves do Nascimento  Ana Sue SammiJoeleni Rosa Dos Santos  Beatriz de Souza Lima NinoAlexey Leon Gomel Bogado  Alessandra TarodaOdilon Vidotto  João Luis Garcia 《Comparative immunology, microbiology and infectious diseases》2014

The aim of the present study was to evaluate anti-Neospora caninum antibodies and the vertical transmission rate in naturally infected pregnant zebu beef cows (Bos indicus) reared on pasture. The present study began with 200 cows from four farms (50 cows from each farm), and these animals were submitted to timed artificial insemination (TAI). After ultrasonography, 76 pregnant cows were selected, 22, 15, 22, and 17, respectively, from farms 1, 2, 3, and 4. Blood samples were taken from cows thrice during the first, second, and third trimester of gestation, and a blood sample was collected from 31 calves before colostrum milking. From 76 cows 23 (30.3%) had anti-N. caninum antibodies detected by indirect ELISA (Idexx), and 53 (69.7%) did not. Sixty-four cows that initiated the experiment were negative to N. caninum and 11 became positive either during the second or third trimester of gestation, this mean an infection incidence of 17.2% (11/64). OD for ELISA was higher (OD = 2.08) during the second and third (OD = 2.10) trimesters of pregnancy when compared with the first (OD = 1.81), however, there were no statistical differences (P = 0.45). The vertical transmission was calculated to be 29.0% (9/31), and the risk of vertical transmission of N. caninum in seropositive dams was 26.25 times higher than seronegative animals (OR = 26.25, 2.38 < OR < 289, P = 0.007). In conclusion, the rate of vertical transmission of N. caninum in pregnant zebu beef cows was 29%, and the risk was 26.25 higher in seropositive dams relative to than seronegative animals.  相似文献   

Effects of dexmedetomidine on pulse pressure variation changes induced by hemorrhage followed by volume replacement in isoflurane‐anesthetized dogs     

Miriely S. Diniz Med Vet  Francisco J. Teixeira‐Neto Med Vet  MSc  PhD  Thaísa D. Cândido Med Vet  Felipe S. Zanuzzo Med Vet  Lívia R. Teixeira Med Vet  MSc  Adriana V. Klein Med Vet  Paulo do Nascimento Jr MSc  PhD 《Journal of Veterinary Emergency and Critical Care》2014,24(6):681-692

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