Evaluation of a 3-dimensional ultrasound device for noninvasive measurement of urinary bladder volume in dogs     

Matthew R. DiFazio  Justin D. Thomason  Natalia Cernicchiaro  David Biller  Sasha Thomason  Paxton Harness 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2020,34(4):1488-1495

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Long-term postoperative pain evaluation in dogs with thoracolumbar intervertebral disk herniation after hemilaminectomy     

Natalia Zidan  Julia Medland  Natasha Olby 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2020,34(4):1547-1555

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Argentinian potato leafroll virus P0 protein: Novel activities for a previously known suppressor     

María P. Barrios Barón  Verónica C. Delfosse  Yamila C. Agrofoglio  Vanesa Nahirñak  Natalia I. Almasia  Cecilia Vazquez Rovere  Ana J. Distéfano 《Plant pathology》2021,70(2):259-274

The potato leafroll virus (PLRV) P0 protein (P0^PL) is a suppressor of RNA silencing. In this study, we showed that P0 protein from an Argentinian isolate of PLRV (P0^PL-Ar) has an additional activity not described for other PLRV or P0 proteins from poleroviruses. Besides reporting that P0^PL-Ar displays both local and systemic silencing suppressor activity, we demonstrated, for the first time, that P0^PL-Ar impedes accumulation of dsRNA-derived siRNAs. We also showed that P0^PL-Ar interacts with Solanum tuberosum SKP1 orthologue (StSKP1) and triggers destabilization of ARGONAUTE 1 (AGO1) and that these actions are mediated by the F-box-like domain. A mutant in the GW/WG motif within the P0^PL-Ar F-box-like motif lost the suppression activity, the interaction with StSKP1 and abolished AGO1 decay. Interestingly, a mutant in the L76/P77 residues within the P0^PL-Ar F-box-like motif, which lost the suppression activity and the interaction with StSKP1, retained the capacity to enable AGO1 decay. Thus, unlike other P0 proteins of previously characterized poleroviruses, P0^PL-Ar seems to have a dual activity, according to the findings of this study. This protein would act at both an upstream and a downstream step of the RNA silencing pathway: upstream of Dicer-like enzyme (DCL)-mediated primary siRNA production and downstream at the RNA-induced silencing complex (RISC) complex level. Our results contribute to the understanding of the different ways PLRV P0 proteins function as silencing suppressors.  相似文献   

Effect of seed bacterization on peroxidase activity in wheat plants when infected with Bipolaris sorokiniana under high temperature and low moisture     

Minaeva  Oksana M.  Zyubanova  Tatyana I.  Akimova  Elena E.  Tereshchenko  Natalia N. 《European journal of plant pathology / European Foundation for Plant Pathology》2022,162(1):79-91

European Journal of Plant Pathology - Sorghum anthracnose caused by the fungal pathogen Colletotrichum sublineolum, is one of the most economically important diseases of sorghum globally and in...  相似文献   

Response of Quercus robur L. seedlings to north-south asymmetry of light within gaps in floodplain forests of Slovenia     

Jurij Diaci  Natalia Gyoerek  Janez Gliha  Tom A. Nagel 《Annals of Forest Science》2008,65(1):105-105

To examine Quercus robur establishment and growth in low to intermediate light levels, we analysed regeneration in different microsites created by light asymmetry within gaps in two main floodplain forest regions of Slovenia: Dolinsko and Krakovo. Four years after a mast year in 1995, we installed systematic grids of 1×1 m plots in 11 gaps (0.03–0.40 ha) on wet and dry site variants. In 256 plots, seedling species, cover, density, height, height increment, browsing damage, tree architecture, understory vegetation species and cover, and direct and diffuse light were measured. The average seedling density in all gaps was 15/m², but the highest densities were found in gap positions with low diffuse light levels (10–20%). Competing understory vegetation was more abundant in positions with high diffuse light. In gaps on dry site variants a combination of low diffuse and high direct light was favourable for regeneration. The results indicated that Q. robur can successfully establish in gaps. Here, both light components were sources of within gap resource heterogeneity, therefore knowledge of light asymmetry can improve regeneration success.  相似文献   

Detection and characterization of Shiga toxin-producing Escherichia coli in captive non-domestic mammals     

Leotta GA  Deza N  Origlia J  Toma C  Chinen I  Miliwebsky E  Iyoda S  Sosa-Estani S  Rivas M 《Veterinary microbiology》2006,118(1-2):151-157

Shiga toxin producing-Escherichia coli (STEC) is an important emerging pathogen, and ruminants are recognized as their main natural reservoir. The aim of this work was to establish the frequency of STEC in non-domestic mammals of the Zoo and Botanical Garden of La Plata City, Argentina, and to pheno-genotypically characterize STEC isolates. By polymerase chain reaction (PCR), Shiga toxin (stx) gene sequences were detected in 50.8% of 65 fecal samples. Twenty-five STEC strains were isolated from 38.5% of the Zoo's animals. Ten species of order Cetartiodactyla and one species of order Rodentia were recognized as new STEC carriers. STEC strains belonged to 7 different serotypes including new serotypes O12:H25 and O13:H6. Serotype O146:H28, previously associated with human infections, represented 24% of STEC isolates. The most frequent Shiga toxin identified were type 1c and type 2c. Nineteen strains were positive for iha gene, 8 strains were positive for ehxA gene. Moreover, all strains were positive for lpfAO113 and negative for rfbO157, eae, saa, lpfAO157/OI-141, lpfAO157/OI-154, efa1, and toxB genes. Results obtained by XbaI-pulsed-field gel electrophoresis (XbaI-PFGE) confirmed the transmission of STEC strains among different animal species and suborders. In addition, we observed a potential association between STEC-harboring animal and factors such as belonging to order Cetartiodactyla, living in a pit, and belonging to a non-autochthonous species. This is the first work developed with zoological mammals and STEC in Argentina.  相似文献   

Effects of age and breed on corneal thickness,density, and morphology of corneal endothelial cells in enucleated sheep eyes          下载免费PDF全文

Natalia Coyo  Maria Teresa Peña  Daniel Costa  José Ríos  Rodrigo Lacerda  Marta Leiva 《Veterinary ophthalmology》2016,19(5):367-372

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Radiographic assessment of cattle hoofs (ungulae) of the Nellore breed: Reference standards     

Maria Cristina Ferrarini Nunes Soares Hage  Rejane dos Santos Sousa  Davi Siqueira Chaves  Raduan Hage  Francisco Leonardo Costa de Oliveira  Mailson Renan Borges Dias  Natalia Sato Minami  Anna Carolina Mazeto Ercolin  Carolina Fortes Cirimbelli  Enrico Lippi Ortolani 《Anatomia, histologia, embryologia》2019,48(2):110-116

The purpose of this work was to determine a radiographic standard of normality for lateral and medial ungulae in 3‐year‐old bovine females of the Nellore breed. Twenty‐six cows underwent radiographic exposure of the medial and lateral ungulae of the hindlimbs and forelimbs; abaxioaxial projections were used. The images were analyzed; distances were measured between the dorsal surface of the distal phalanx and the surface of the hoof capsule. The two angles formed, i.e., an angle between the dorsal surface of the distal phalanx and a line parallel to the sole (inner), and an angle between the dorsal surface of the hoof and a line parallel to the sole (outer), were also recorded. The results suggested a standard of normality for the proximal, middle and distal distances of the ungulae of the forelimbs: 0.96 ± 0.12 cm. For the measurement of hindlimbs, except for the distal distances of the side ungulae, the suggested value was 1.02 ± 0.14 cm; for the distal measurement of the lateral ungulae of the hindlimbs, the value was 1.09 ± 0.12 cm. For inner and outer angles, the values were 36.45° ± 4.4° for the medial ungulae of the forelimbs and 42.50° ± 3.8° for other ungulae of the forelimbs and hindlimbs (standard deviations of the values suggested for the angles should be used as variations between different animals, limbs and ungulae; they should not be used between angles of the same ungula).  相似文献   

The effect of single pretreatment with salbutamol on recovery of bronchoalveolar lavage fluid in horses with suspected or confirmed severe equine asthma     

Mathilde S. Varegg  Kine M. Klverd  Malin K. Austnes  Natalia Siwinska  Malwina Slowikowska  Agnieszka Zak  Artur Niedzwiedz 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(2):976-980

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Predisposing factors for bacterial mastitis in ewes     

Natalia G. C. Vasileiou  Vassiliki S. Mavrogianni  Efi Petinaki  George C. Fthenakis 《Reproduction in domestic animals》2019,54(10):1424-1431

Objective of this review paper is the appraisal of predisposing factors for bacterial mastitis in ewes. Factors that predispose ewes to mastitis can be classified into non‐animal‐related factors (environmental and climatological factors, housing, nutrition, milking practices) and animal‐related factors (anatomic, genetic, litter size—ethological factors, number and stage of lactation period, health problems). There are clear management—environment—animal interactions in mastitis development in ewes, which underline its multifactorial nature. Research and studies regarding risk factors are important, in order to develop strategies for their elimination, control or correction. Control measures for bacterial mastitis, which attempt to eliminate predisposing factors for infection, can thus be developed and implemented.  相似文献