Molecular cloning of canine mucosal addressin cell adhesion molecule-1 cDNA and its expression in normal tissues     

Miura T  Watari T  Maruyama H  Sakai M  Takahashi T  Kano R  Koie H  Yamaya Y  Tokuriki M  Hasegawa A 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(12):1257-1260

A cDNA encoding canine mucosal addressin cell adhesion molecule-1 (MAdCAM-1) was cloned. The entire open reading frame of canine MAdCAM-1 cDNA comprises 1137 bp, corresponding to 378 amino acid residues. The deduced amino acid sequence of canine MAdCAM-1 was 55.2%, 53.7%, and 52.4% identical to rat, mouse, and human MAdCAM-1, respectively. Canine MAdCAM-1 appeared to contain two immunoglobulin-like domains at the N-terminus, followed by a mucin-like domain and a third immunoglobulin-like domain. The structures of the dog, rat, and mouse proteins are likely similar because all of the cysteine residues in the immunoglobulin-like domains were conserved. Canine MAdCAM-1 mRNA was confirmed to express extremely in the mesenteric lymph node by RT-PCR.  相似文献   

Cloning of canine Toll-like receptor 7 gene and its expression in dog tissues   总被引：1，自引：0，他引：1  

Okui Y  Kano R  Maruyama H  Hasegawa A 《Veterinary immunology and immunopathology》2008,121(1-2):156-160

Toll-like receptor 7 (TLR7) is activated by single strand RNA and imidazoquinoline compounds, and induces interferon production. In this study, canine TLR7 cDNA was cloned and sequenced. The full-length cDNA of canine TLR7 gene was 3419bp, encoding 1032 amino acids. The similarities of canine TLR7 with human and mouse TLR7 were 84 and 80% at the nucleotide sequence level, and 86 and 79% at amino acid sequence level, respectively. Further, the expression of TLR7 mRNA was investigated in canine normal tissues by semiquantitative RT-PCR analysis. The common expression level of TLR7 mRNA in tissues from three dogs examined was in large intestine, lung, pancreas, small intestine and skin, though the expression level in each tissue was varied among these healthy dogs. In other tissues (kidney, liver, lymph node, spleen, adrenal gland, and PBMCs), the level of TLR7 mRNA expression was different in individuals.  相似文献   

Inosine‐5'‐monophosphate is a candidate agent to resolve rigor mortis of skeletal muscle          下载免费PDF全文

Masanori Matsuishi  Mariko Tsuji  Megumi Yamaguchi  Natsumi Kitamura  Sachi Tanaka  Yukinobu Nakamura  Akihiro Okitani 《Animal Science Journal》2016,87(11):1407-1412

The object of the present study was to reveal the action of inosine‐5'‐monophosphate (IMP) toward myofibrils in postmortem muscles. IMP solubilized isolated actomyosin within a narrow range of KCl concentration, 0.19‐0.20 mol/L, because of the dissociation of actomyosin into actin and myosin, but it did not solubilize the proteins in myofibrils with 0.2 mol/L KCl. However, IMP could solubilize both proteins in myofibrils with 0.2 mol/L KCl in the presence of 1 m mol/L pyrophosphate or 1.0–3.3 m mol/L adenosine‐5'‐diphosphate (ADP). Thus, we presumed that pyrophosphate and ADP released thin filaments composed of actin, and thick filaments composed of myosin from restraints of myofibrils, and then both filaments were solubilized through the IMP‐induced dissociation of actomyosin. Thus, we concluded that IMP is a candidate agent to resolve rigor mortis because of its ability to break the association between thick and thin filaments.  相似文献   

Effects of Increasing Temperatures on Spikelet Fertility in Different Rice Cultivars based on Temperature Gradient Chamber Experiments     

A. Maruyama  W. M. W. Weerakoon  Y. Wakiyama  K. Ohba 《Journal of Agronomy and Crop Science》2013,199(6):416-423

Spikelet sterility in rice (Oryza sativa L.) induced by high temperatures is a major concern given global warming predictions. We studied differences among eight rice cultivars in spikelet fertility at five different temperature levels in temperature gradient chamber (TGC) experiments. Six japonica and two indica cultivars were exposed to high‐temperature gradients in TGCs during the 2005 flowering season. Spikelet sterility increased with temperature in TGCs and differed among cultivars because of both variations in temperature tolerance and timing of heading. The correlation between spikelet fertility of individual panicles and both air temperature and panicle temperature during flowering was analyzed to compare tolerances among cultivars. The temperature (T₇₅) at which spikelet fertility was 75 % of maximum ranged from 34 to 39 °C air temperature and differed significantly among cultivars. Indica varieties had higher T₇₅ values than japonica varieties. The T₇₅ values based on panicle temperature also differed among cultivars, but the difference between indica and japonica varieties were less significant. We concluded that the higher temperature tolerances of indica cultivars in our experiments could be attributed to lower spikelet temperatures, and cultivars with similar spikelet temperatures still had different heat tolerances due to differences in pollination ability.  相似文献   

An economic evaluation of Kanazawa and Shichika irrigation water’s multi-functional roles using CVM     

Maruyama Toshisuke  Takimoto Hiroshi 《Paddy and Water Environment》2008,6(3):309-318

In order to confirm that the regional function of Kanazawa’s irrigation water is representative of the urban type, and that Shichika’s irrigation water is representative of the rural, the contingent valuation method (CVM) was applied to determine the economic value of the functions aside from agricultural production, that is, the multi-functional roles of the irrigation water. The economic value of the regional function in Kanazawa was significantly lower than in the Shichika area, reflecting a difference in charge for irrigation water and people’s preference or consciousness in urban and rural areas. In urban areas, people tend to place a higher value on ground view, peace of mind and environmental friendliness. In contrast, in rural areas, irrigation water is presently used for domestic purposes. In other words, in urban areas people see water as public goods, while in rural areas the people see water as private goods. In addition, differences in the economic value of each item that was statistically significant for CVM were estimated using the Turnbull model comparing the items approved or rejected by the respondent.  相似文献   

Complete replication of hepatitis C virus in cell culture     

Lindenbach BD  Evans MJ  Syder AJ  Wölk B  Tellinghuisen TL  Liu CC  Maruyama T  Hynes RO  Burton DR  McKeating JA  Rice CM 《Science (New York, N.Y.)》2005,309(5734):623-626

Many aspects of the hepatitis C virus (HCV) life cycle have not been reproduced in cell culture, which has slowed research progress on this important human pathogen. Here, we describe a full-length HCV genome that replicates and produces virus particles that are infectious in cell culture (HCVcc). Replication of HCVcc was robust, producing nearly 10(5) infectious units per milliliter within 48 hours. Virus particles were filterable and neutralized with a monoclonal antibody against the viral glycoprotein E2. Viral entry was dependent on cellular expression of a putative HCV receptor, CD81. HCVcc replication was inhibited by interferon-alpha and by several HCV-specific antiviral compounds, suggesting that this in vitro system will aid in the search for improved antivirals.  相似文献   

Chemical interesterification of blends of palm stearin, coconut oil, and canola oil: physicochemical properties     

Soares FA  da Silva RC  Hazzan M  Capacla IR  Viccola ER  Maruyama JM  Gioielli LA 《Journal of agricultural and food chemistry》2012,60(6):1461-1469

trans-Free interesterified fat was produced for possible usage as a margarine. Palm stearin, coconut oil, and canola oil were used as substrates for chemical interesterification. The main aim of the present study was to evaluate the physicochemical properties of blends of palm stearin, coconut oil, and canola oil submitted to chemical interesterification using sodium methoxide as the catalyst. The original and interesterified blends were examined for fatty acid composition, softening and melting points, solid fat content, and consistency. Chemical interesterification reduced softening and melting points, consistency, and solid fat content. The interesterified fats showed desirable physicochemical properties for possible use as a margarine. Therefore, our result suggested that the interesterified fat without trans-fatty acids could be used as an alternative to partially hydrogenated fat.  相似文献   

Analysis of water balance in the Tedori river alluvial fan areas of Japan: focused on quantitative analysis of groundwater recharge from river and ground surface, especially paddy fields     

Toshisuke Maruyama  Fumikazu Noto  Masashi Yoshida  Haryuhiko Horino  Kimihito Nakamura 《Paddy and Water Environment》2014,12(1):163-171

Water balance in the Tedori River alluvial fan areas was analyzed for all components of the hydrological cycle based on exchange of the channel/soil surface and aquifer horizon fractions with river water. The results were summarized on an annual basis, as well as for the irrigation and non-irrigation periods. The study area received 6.28 mm/day of precipitation and had an outflow of 2.32 mm/day as direct runoff, resulting in 3.96 mm/day of water being supplied to the soil surface. The channel/soil horizon fraction received this 3.96 mm/day, as well as 9.12 mm/day intake water from the head works. Conversely, 2.74 and 2.85 mm/day were lost by evapotranspiration and percolation, respectively. Thus, surface runoff of 7.49 mm/day flowed from the study area to the Sea of Japan or drainage canals near the river mouth. In the aquifer horizon fraction, 2.85 mm/day of water was supplied from the channel/soil horizon fraction and 2.15 mm/day was supplied from the Tedori River, while 1.73 mm/day was extracted by groundwater. Thus, 3.27 mm/day of groundwater flowed out to the Sea of Japan or into downstream drainage canals. An outline of the water balance of the irrigation and non-irrigation period is also shown. Because various hydrological components are closely related to each other, planning and management of water resources for individual goals are not adequate, but require the integrated aspect of water balance for sustainable water use.  相似文献   

Erratum to: Ureaplasma diversum as a cause of pustular vulvovaginitis in bovine females in Vale Guapore,Mato Grosso State,Brazil     

João Guilherme L. N. Gaeti  Marconni V. C. Lana  Gustavo S. Silva  Leticya L. Lopes  Camila G. de Campos  Fernanda H. Maruyama  Edson M. Colodel  Eduardo F. Costa  Luis G. Corbellini  Luciano Nakazato  Caroline A. Pescador 《Tropical animal health and production》2014,46(6):1065-1065

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Epidemiological Analysis of Methicillin-Resistant Staphylococcus aureus Carriage among Veterinary Staff of Companion Animals in Japan     

Kanako ISHIHARA  Mieko SAITO  Natsumi SHIMOKUBO  Yasukazu MURAMATSU  Shigeki MAETANI  Yutaka TAMURA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(12):1627-1629

Veterinary staff carrying methicillin-resistant Staphylococcus aureus(MRSA) can be a source of MRSA infection in animals. To identify risk factors of MRSA carriage among veterinary staff, MRSA carriage and epidemiological information (sex, career, contact with MRSA-identified animal patients and others) were analyzed from 96 veterinarians and 70 veterinary technicians working at 71 private veterinary clinics in Japan. Univariate analysis determined sex (percentage of MRSA carriage, male (29.2%) vs. female (10%); P=0.002) and career (veterinarians (22.9%) vs. veterinary technicians (10%); P=0.030) as risk factors. Multivariable analysis revealed that sex was independently associated with MRSA carriage (adjusted odds ratio, 3.717; 95% confidence interval, 1.555–8.889; P=0.003). Therefore, male veterinary staff had a higher risk of MRSA carriage than female staff.  相似文献