Epidemiology of Oestrus ovis (Linneo, 1761) infestation in goats in Spain     

Alcaide M  Reina D  Frontera E  Navarrete I 《Veterinary parasitology》2005,130(3-4):277-284

This survey was conducted to determine the chronobiology and seroprevalence of nasal bot infestation (Oestrus ovis) in Spain and to identify the risk factors associated with this disease in caprine herds.

A total of 1590 sera from adult goats were collected at random on 175 farms in southwestern Spain. Sera were tested by ELISA, using crude protein from second stage larvae as antigen. The mean seroprevalence was 46.04% and mean percentage of optical densities was 41.83. These data indicate a high prevalence of this parasite in the investigated areas. The serological survey revealed that goats managed at higher altitudes, at meridians latitudes and on farms with small herds had a smaller probability of infestation. Eighty goat heads, obtained from abattoirs in the central region of Spain, were collected and examined for nasal botflies from February to October 2002. O. ovis larval stages were recovered from the nasal-sinus cavities of 23 goats, reaching a prevalence of 34.94%. The mean larval burden was 3.9 larvae per infested head. No first instars were found during February and March, when the second instar reached its larger count. The third instar was observed in very small number during the whole period of study, with one peak occurring in July–August. These data show the existence of a favourable period for the development of larval instars of O. ovis in goats that starts in February and finishes in September.  相似文献   

一例犬细小病毒病的诊断与治疗   总被引：3，自引：0，他引：3  

齐静  李爱军 《中国兽药杂志》2005,39(12):50-51

犬细小病毒病是由犬细小病毒引起的以出血性肠炎或非化脓性心肌炎为主要特征的一类疾病.根据发病情况、临床特征、剖检变化、实验室诊断等情况,对一例犬细小病毒病临床病例进行了诊断并采取了相应的治疗措施,取得了较好的效果.  相似文献   

Resistance to glyphosate in Lolium rigidum     

Debrah F Lorraine-Colwill  Tim R Hawkes  Patricia H Williams  Simon AJ Warner  Peter B Sutton  Stephen B Powles  Christopher Preston 《Pest management science》1999,55(4):489-491

Annual ryegrass (Lolium rigidum) is a widespread and important weed of Australia and populations of this weed have developed resistance to most major herbicides, including glyphosate. The possible mechanisms of resistance have been examined in one glyphosate-resistant Lolium population. No major differences were observed between resistant and susceptible biotypes in respect of (i) the target enzyme (EPSP synthase), (ii) DAHP synthase, the first enzyme of the target (shikimate) pathway, (iii) absorption of glyphosate, or (iv) translocation. Following treatment with glyphosate, there was greater accumulation of shikimate (derived from shikimate-3-Pi) in susceptible than in resistant plants. In addition, the resistant population exhibited cross-resistance to 2-hydroxy-3-(1,2,4-triazol-1-yl)propyl phosphonate, a herbicide which, although structurally similar to glyphosate, acts at an unrelated target site. On the basis of these observations we speculate that movement of glyphosate to its site of action in the plastid is involved in the resistance mechanism. © 1999 Society of Chemical Industry  相似文献   

Influence of Raw Materials and Processing Conditions on Spaghetti Hydration Kinetic During Cooking and Overcooking     

M. A. Del Nobile  C. Fares  A. Conte  N. Martinez‐Navarrete  G. Mocci  E. La Notte 《Cereal Chemistry》2003,80(5):601-607

A study of the influence of raw materials and processing conditions on the hydration kinetic during cooking and overcooking of spaghetti is presented. Water sorption tests on commercially available spaghetti and on three different types of homemade spaghetti were run at 100°C. In particular, the weight, diameter, and length of spaghetti strands were monitored over time for ≈200 min. The spaghetti hydration process was quantitatively resolved into the controlling phenomena (i.e., water diffusion, macromolecular matrix relaxation kinetic, and residual deformation release kinetic) by fitting a novel mathematical model to the experimental data. The different behavior of spaghetti was analyzed for each of the phenomena involved during hydration. Results suggest that processing conditions highly influence the quality of spaghetti with respect to raw materials.  相似文献   

An α‐amylase‐like protein interacts with PirB toxin from Vibrio parahaemolyticus in digestive tract tissue of white shrimp Litopenaeus vannamei     

Norineli Almanza‐Martínez  Sergio Francisco Martínez Díaz  Gabriela Flores‐Ramírez  Fernando Zuiga‐Navarrete  Isabel Gmez  Csar Salvador Cardona‐Flix 《Aquaculture Research》2020,51(9):3910-3914

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Activity,molecular mass and hydrolysis on baker's yeast protein of extracellular proteases from the putative probiotic bacteria Microbacterium sp. strain 8L and Exiguobacterium mexicanum strain 8N     

César Orozco‐Medina  Alejandro M Maeda‐Martínez  Julio Humberto Córdova‐Murueta  Alejandro López‐Cortés  María de los Ángeles Navarrete‐del‐Toro 《Aquaculture Research》2009,41(1):45-52

The bacteria Microbacterium sp. 8L and Exiguobacterium mexicanum 8N are known to improve the culture of Artemia franciscana using baker's yeast as food. Using spectrophotometry, sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS‐PAGE), substrate‐SDS‐PAGE and pH‐stat in vitro‐digestibility assays, the activity, molecular mass and hydrolysis on baker's yeast protein of proteases from extracellular polymeric substances (EPS) of the strains 8L and 8N along with the pathogenic strains Microbacterium sp. 8R and Vibrio parahaemolyticus 588 CECT (Vp) were studied. The EPSs of 8L and 8R showed one activity band, on which the serine inhibitor phenylmethylsulphonyl fluoride (PMSF) had no effect. The EPSs of 8N showed four bands; two were unaffected by PMSF, whereas one was affected, and the other was partially affected. The EPSs of Vp showed two bands, one partially inhibited by PMSF. No inhibitory effects from 1‐chloro‐3‐tosylamido‐7‐amino‐2‐heptanone (trypsin inhibitor) were observed in the protease bands of the studied bacteria. The EPSs of 8L and 8N showed a similar degree of hydrolysis (pH‐stat). The EPSs of 8L had the lowest Dice index of similarity of yeast protein profiles at 1 h of reaction. We conclude that the strain 8L could benefit A. franciscana by providing bacterial proteases for digestion of baker's yeast.  相似文献   

Importance of Endometrial Luteinizing Hormone Receptors in Induction of Luteolysis and Maternal Recognition of Pregnancy in the Pig   总被引：5，自引：0，他引：5  

AJ Ziecik  A Stepien  B Gawronska 《Reproduction in domestic animals》2000,35(3-4):190-192

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The geographic distribution of hydatid infection in cattle in Western Australia Western Australia     

AJ LYMBERY  RCA THOMPSON  CC CONSTANTINE  JG KRUGER 《Australian veterinary journal》1995,72(11):430-432

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Expression of Cyclooxygenase-1 and -2 in the Porcine Endometrium during the Oestrous Cycle and Early Pregnancy     

A Blitek  A Waclawik  MM Kaczmarek  T Stadejek  Z Pejsak  AJ Ziecik 《Reproduction in domestic animals》2006,41(3):251-257

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Role of Tumour Necrosis Factor α in Stimulation of Prostaglandins F_2α and E₂ Release by Cultured Porcine Endometrial Cells     

A Blitek  AJ Ziecik 《Reproduction in domestic animals》2006,41(6):562-567

The present studies were undertaken to examine the effect of tumour necrosis factor (TNF) alpha on prostaglandins (PGs) F(2alpha) and E(2) release by cultured porcine endometrial cells harvested on days 13-16 after oestrus in comparison to stimulation with oxytocin (OT) and luteinizing hormone (LH). A time-dependent effect of TNFalpha (10 ng/ml) on PGF(2alpha) release was observed in stromal and luminal epithelial cells. Moreover, TNFalpha increased PGF(2alpha) secretion from both endometrial cell types with effective concentrations of 1 (p < 0.05), 10 and 50 ng/ml (p < 0.01). The effect of TNFalpha (10 ng/ml) on endometrial PGF(2alpha) and PGE(2) release was compared with OT (100 nmol/l) and LH (100 ng/ml). All factors affected PGF(2alpha) secretion from stromal cells, however, the stimulation tended to be more potent after OT and LH (p < 0.01) than after TNFalpha (p < 0.05) treatment. In epithelial cells, only TNFalpha was able to stimulate PGF(2alpha) release (p < 0.001). PGE(2) secretion from stromal cells increased after incubation with TNFalpha and OT (p < 0.05). Only LH stimulated PGE(2) release from epithelium (p < 0.001), and its action was very effective when compared with TNFalpha or OT (p < 0.01). Summarizing, TNFalpha induces both PGs secretion from cultured porcine endometrium, but preferentially stimulates PGF(2alpha) release from luminal epithelial cells. Therefore, similarly to OT and LH, TNFalpha may be considered as a potential modulator of endometrial PGF(2alpha) production during luteolysis in the pig.  相似文献