Furazolidone toxicity in dairy calves     

JD TAYLOR  JA GIBSON  CEF YEATES 《Australian veterinary journal》1991,68(5):182-183

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Cardiomyopathy and woolly haircoat syndrome of Hereford cattle     

GJ STORIE  JA GIBSON  JD TAYLOR 《Australian veterinary journal》1991,68(3):119-119

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Papillomatous digital dermatitis spirochetes suppress the bovine macrophage innate immune response   总被引：1，自引：0，他引：1  

Zuerner RL  Heidari M  Elliott MK  Alt DP  Neill JD 《Veterinary microbiology》2007,125(3-4):256-264

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Genetic diversity of ruminant <Emphasis Type="Italic">Pestivirus</Emphasis> strains collected in Northern Ireland between 1999 and 2011 and the role of live ruminant imports     

Maria P. Guelbenzu-Gonzalo  Lynsey Cooper  Craig Brown  Sam Leinster  Ronan O’Neill  Liam Doyle  David A. Graham 《Irish veterinary journal》2015,69(1):7

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Estimating the mass of asteroid 253 mathilde from tracking data during the NEAR flyby   总被引：1，自引：0，他引：1  

DK Yeomans  J Barriot  DW Dunham  RW Farquhar  JD Giorgini  CE Helfrich  AS Konopliv  JV McAdams  JK Miller  WM Owen  DJ Scheeres  SP Synnott  BG Williams 《Science (New York, N.Y.)》1997,278(5346):2106-2109

The terminal navigation of the Near Earth Asteroid Rendezvous (NEAR) spacecraft during its close flyby of asteroid 253 Mathilde involved coordinated efforts to determine the heliocentric orbits of the spacecraft and Mathilde and then to determine the relative trajectory of the spacecraft with respect to Mathilde. The gravitational perturbation of Mathilde on the passing spacecraft was apparent in the spacecraft tracking data. As a result of the accurate targeting achieved, these data could be used to determine Mathilde's mass as 1.033 (+/- 0.044) x 10(20) grams. Coupled with a volume estimate provided by the NEAR imaging team, this mass suggests a low bulk density for Mathilde of 1.3 grams per cubic centimeter.  相似文献   

Identification of the major proteins of an immune modulating fraction from adult Fasciola hepatica released by Nonidet P40     

Russell M. Morphew  Clare M. Hamilton  Hazel A. Wright  David J. Dowling  Sandra M. O’Neill  Peter M. Brophy 《Veterinary parasitology》2013,191(3-4):379-385

Fasciola hepatica NP-40 released protein extract (FhNPE) exhibits potent Th1 immunosuppressive properties in vitro and in vivo. However, the protein composition of this active fraction, responsible for Th1 immune modulatory activity, has yet to be resolved. Therefore, FhNPE, a Nonidet P-40 extract, was subjected to a proteomic analysis in order to identify individual protein components. This was performed using an in house F. hepatica EST database following 2D electrophoresis combined with de novo sequencing based mass spectrometry. The identified proteins, a mixture of excretory/secretory and membrane-associated proteins, are associated with stress response and chaperoning, energy metabolism and cytoskeletal components. The immune modulatory properties of these identified protein(s) are discussed and HSP70 from F. hepatica is highlighted as a potential host immune modulator for future study.  相似文献   

Rapid,effective and user-friendly immunophenotyping of canine lymphoma using a personal flow cytometer     

Stratos Papakonstantinou  Inese Berzina  Amanda Lawlor  Emma J O’Neill  Peter J O’Brien 《Irish veterinary journal》2013,66(1):6

Background

Widespread use of flow cytometry for immunophenotyping in clinical veterinary medicine is limited by cost and requirement for considerable laboratory space, staff time, and expertise. The Guava EasyCyte Plus (Guava Technologies, Hayward, CA, US) is the first, personal, bench-top flow cytometer designed to address these limitations.

Objective

The aim of this study was to adapt the immunohistochemical protocol used for immunophenotyping of canine lymphoma to the personal flow cytometer for rapid, effective and user-friendly application to the diagnosis and prognosis of canine lymphoma and to demonstrate its practicality for widespread veterinary application. Performance of the personal flow cytometer for immunophenotyping T and B lymphocytes in blood and lymph nodes from normal dogs and dogs with lymphoproliferative disease, was assessed using only two monoclonal antibodies (against CD3 and CD21), and by comparison with analysis using two conventional flow cytometers.

Methods

26 dogs with lymphoproliferative disease (23 with lymphoma, 3 with lymphocytic leukaemia) were studied along with 15 controls (2 non-lymphoma lymph nodes and 13 non-leukemic bloods. Lymphocytes were immunostained with fluorescent-labeled, monoclonal antibodies against CD3 and CD21. To assess the effectiveness of the personal flow cytometer in discrimination between T and B cell immunophenotypes, T and B cell counts for half the samples (14 blood and 11 lymph node) were also determined using the same method and conventional flow cytometers (FACSCalibur, Cyan Dako). To assess the effectiveness of the personal flow cytometer in discriminating between leukocyte types, lymphocyte differential counts were determined for 21 blood samples and compared with those from automated hematology analyzers (CELL-DYN 3500, n=11 and ADVIA 2120, n=10). Quality and sub-cellular distribution of immunostaining was assessed using fluorescence microscopy.

Results

The protocol for immunophenotyping took 2 to 3 hours to complete from the point of receipt of sample to reporting of immunophenotype. The personal flow cytometer differential lymphocyte counts correlated highly (n=20; r=0.97, p<0.0001) with those of automated haematology analyzers. The personal flow cytometer counts consistently, but mildly, underestimated the percentages of lymphocytes in the samples (mean bias of -5.3%.). The personal flow cytometer immunophenotype counts were indistinguishable from those of conventional flow cytometers for both peripheral blood samples (n=13; r=0.95; p<0.0001; bias of -1.1%) and lymph node aspirates (n=11,r=0.98; p<0.001; bias of 1%). All but one leukemic and one lymphomatous lymph node sample, out of 26 samples of dogs with lymphoproliferative disease analyzed, could be immunophenotyped as either B or T cells.

Conclusions

We conclude that use of only 2 monoclonal antibodies is sufficient for immunophenotyping most cases of canine lymphoma by flow cytometry and enables rapid immunophenotyping. The personal flow cytometer may be as effectively used for immunophenotyping canine lymphoma as conventional flow cytometers. However, the personal flow cytometer is more accessible and user-friendly, and requires lower sample volumes.  相似文献   

Testosterone serum profile,semen characteristics and testicular biometry of Mangalarga Marchador stallions in a tropical environment          下载免费PDF全文

B Waddington  JM Penitente‐Filho  JGS Neves  RO Pinho  AY Chaya  PP Maitan  CO Silveira  MG Neves  SEF Guimarães  GR de Carvalho  JD Guimarães 《Reproduction in domestic animals》2017,52(2):335-343

This study was conducted to characterize the daily profile of testosterone secretion and its mean concentrations in the four seasons as well as to evaluate the semen characteristics and testicular biometry of Mangalarga Marchador stallions throughout the year in a tropical region. Three stallions were submitted to semen collections and evaluation of testicular biometry every 14 days along a year. Blood samples were collected once at the middle of each season, in a 20‐min interval during 24 hr in order to evaluate the testosterone secretion profiles among seasons. Testosterone concentrations along the day were higher at the beginning of the afternoon (from 12:00 to 15:00 hr), but a circadian secretion was not clearly observed. Mean testosterone concentrations did not differ among seasons (p > .05), but a pattern of secretion along the day showed variations with higher concentrations in the afternoon during the winter. Ejaculate volume was higher during summer; however, sperm motility decreased in summer and spring. Total sperm in ejaculate, sperm morphology and testicular biometry kept constant along the year showing no differences among the seasons. The results demonstrated that in a tropical region, reproductive aspects of stallions did not show a clearly defined seasonal variation, and months of autumn and winter were not unsuitable for reproduction of the males.  相似文献   

Response of sorghum stalk pathogens to <Emphasis Type="Italic">brown midrib</Emphasis> plants and soluble phenolic extracts from near isogenic lines     

Deanna L. Funnell-Harris  Patrick M. O’Neill  Scott E. Sattler  Tammy Gries  Mark A. Berhow  Jeffrey F. Pedersen 《European journal of plant pathology / European Foundation for Plant Pathology》2017,148(4):941-953

Sorghum [Sorghum bicolor (L.) Moench] has drawn attention as potential feedstock for lignocellulosic biofuels production, and reducing lignin is one way to increase conversion efficiency. Little research has been previously conducted to assess the response of reduced lignin sorghum lines to the Fusarium stalk rot pathogens Fusarium verticillioides and Fusarium proliferatum and the charcoal rot pathogen, Macrophomina phaseolina. Loss of function mutations in either the Brown midrib (Bmr) 6 or 12 gene that both encode a monolignol biosynthetic enzyme in the pathway that produces subunits of the lignin polymer, results in reduced lignin content. Near-isogenic bmr6, bmr12, and bmr6 bmr12 lines had previously been developed, which were shown to have significantly reduced lignin content and increased levels of soluble phenolics. In the current study, these lines in two backgrounds were shown to not be more susceptible to F. verticillioides, F. proliferatum and M. phaseolina inoculations, and some bmr lines exhibited increased resistance to F. proliferatum and M. phaseolina, compared to wild-type lines. When the Fusarium stalk rot pathogen, Fusarium thapsinum, was grown on methanol soluble stalk extracts from bmr6 and wild-type plants, it grew significantly faster on medium with bmr6 extract than on wild-type extract or controls. This result suggested that factors other than soluble phenolics from the extract, such as cell wall bound phenolics or inducible defense compounds, contributed to increased resistance observed in bmr6 plants.  相似文献   

Robert Biel: Sustainable food systems: the role of the city     

Kirstie O’Neill 《Agriculture and Human Values》2018,35(4):911-912

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