Synovial Prostaglandin E2 Concentrations in Horses Fed Long Chain Polyunsaturated Fatty Acids: An Inquiry Study     

T. Ross  T. Hess  D. Hansen  T. Engle  N. Schauermann  J. Rexford 《Journal of Equine Veterinary Science》2011,31(5-6):270-271

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Evaluation of phagocytosis, bactericidal activity, and production of superoxide anion, nitric oxide, and tumor necrosis factor-alpha in Kupffer cells of neonatal pigs.     

J K Akunda  F A Ahrens  T T Kramer 《American journal of veterinary research》2001,62(7):1040-1045

OBJECTIVE: To evaluate the activity of Kupffer cells (KC) of control neonatal pigs and neonatal pigs treated with endotoxin and to compare activity of KC with that of pulmonary alveolar macrophages (PAM). SAMPLE POPULATION: Kupffer cells and PAM obtained from 24 neonatal pigs (7 to 10 days old). PROCEDURE: Pairs (n = 7) of littermates served as treated (lipopolysaccharide [LPS]) or untreated pigs. Pigs were euthanatized 24 hours after treatment, and cells were isolated. Cells were obtained from 10 other neonatal pigs for other assays. Functional activity of cells was evaluated by use of in vitro assays to evaluate bactericidal activity, phagocytosis, and production of superoxide anion (SOA), nitric oxide (NO), and tumor necrosis factor-alpha (TNF-alpha). Each assay was repeated on cells obtained from 4 to 6 pigs. RESULTS: Phagocytic activity was similar in KC and PAM, but bactericidal activity and production of SDA and TNF-alpha was lower in KC. Neither KC nor PAM produced NO in response to LPS stimulation. Phagocytosis, bactericidal activity, and production of SOA were enhanced for KC obtained from neonatal pigs treated with LPS. The PAM from LPS-treated neonatal pigs had similar bactericidal activity to PAM obtained from untreated pigs. CONCLUSIONS AND CLINICAL RELEVANCE: Functional capacity of KC is affected by endotoxin. This provides additional information of the role the liver plays in immune surveillance. In addition, the response of KC in neonatal pigs exposed to endotoxin is of value for understanding gram-negative bacterial sepsis, which is a major cause of mortality in neonatal pigs.  相似文献   

The fluorescent antibody test as a rapid method for the demonstration of Bacillus larvae     

W Ahrens 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1974,21(5):385-388

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Mycoplasma hyosynoviae isolation from the upper respiratory tract and tonsils of pigs.   总被引：2，自引：0，他引：2  

N F Friis  P Ahrens  H Larsen 《Acta veterinaria Scandinavica》1991,32(4):425-429

The occurrence of Mycoplasma hyosynoviae at different locations of the upper respiratory tract and tonsils of pigs was investigated in herds with problems of arthritis apparently caused by this microorganism. The isolation of M. hyosynoviae was facilitated by the use of a medium selectively suppressing the growth of Mycoplasma hyorhinis. M. hyosynoviae was cultured from 106 of 178 tonsils of slaughterhouse pigs from 8 herds but could not be isolated from the mucosa of the nasal cavity or the oral-pharyngeal area of 100 living, 10-20 weeks old pigs in 5 of the herds. The value of the selective principles in the medium appears from the circumstance that 86 of the 106 isolates were obtained despite the presence of M. hyorhinis. It is concluded that the tonsil is a reservoir for M. hyosynoviae and is probably the location of choice for an easy demonstration of the presence of this mycoplasma in a pig herd.  相似文献   

Long-term land-use change effects on phosphorus fractionation in Zrêbar Lake margin soils     

Mohsen Sheklabadi  Hamid Mahmoudzadeh  Ali Akbar Mahboubi  Bahram Gharabaghi  Beau Ahrens 《Archives of Agronomy and Soil Science》2013,59(6):737-749

The aim of this study was to survey long-term changes in phosphorus (P) fractions in soils in different land uses. Sequential extraction had been used to determine soil P fractionation on the basis of land-use change, i.e. native forest to vineyard and wetland to both alfalfa and wheat at the end of 30 and 20 years, respectively. The highest values of labile-P (L-P) fractions in surface soil layers were observed in the cultivated land. The calcium-bound P was the most affected fraction by land-use change with the lowest amount in the vineyard and the highest amount in the alfalfa land. Conversion of forest to vineyards causes P in the soils to be more vulnerable to transmission; thus, the least amount of total P (T-P) in vineyard may be attributed to the removal of sediment and sediment bond P from runoff in response to land-use changes. Average L-P in alfalfa land was two times more than that in vineyard. Results showed that 44.5% of T-P in native land was stored in surface layer; besides, the portion of the arable surface horizon in T-P sequestration was 33%.  相似文献   

Tracer studies of urea kinetics in growing pigs: II. The effect of starch infusion at the distal ileum on urea recycling and bacterial nitrogen excretion.     

R Mosenthin  W C Sauer  H Henkel  F Ahrens  C F de Lange 《Journal of animal science》1992,70(11):3467-3472

Six gilts, with an average BW of 70 kg, were fitted with a simple T-cannula at the distal ileum to study the effect of continuous starch infusion on urea kinetics by means of a radioisotope dilution technique. The pigs were fed twice daily 600 g of a cornstarch-based diet formulated to contain 16% CP by supplementation with isolated soy protein. Infusion of starch, compared with water, decreased (P < .05) plasma urea concentration, urea pool size, and entry, excretion, and degradation rates; urea turnover rate and urea space were not affected (P > .05). Expressed as a percentage of total entry rate, approximately 40% of urea was recycled into the digestive tract in both infusion treatments. The stimulation of microbial fermentation in the large intestine resulted in an increase (P < .05) in fecal N excretion, which was mainly due to an increased excretion of bacterial N. This increase could not be attributed to a greater secretion of urea into the large intestine and its subsequent utilization by the intestinal microflora. The increased bacterial N assimilation after starch infusion led to a reduction in ammonia absorption from the large intestine, which in turn was reflected by a reduced urinary N excretion. As a result, the overall N balance was not affected. In a second experiment, two barrows, with an average BW of 80 kg, were fed twice daily 1.4 kg of a cereal-based diet. The body urea pool of both pigs was labeled with a single injection of 1 g and 2 g of [15N]urea, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

Efficacy of amitraz (Taktic 12.5% EC) as a dip for the control of Boophilus microplus (Canestrini) (Acari: Ixodidae) on cattle     

J. E. George  R. B. Davey  E. H. Ahrens  J. M. Pound  R. O. Drummond 《Preventive veterinary medicine》1998,37(1-4):55-67

Four groups of cattle infested with Boophilus microplus (Canestrini) were each dipped in a different concentration of amitraz diluted from a 12.5% EC formulation to determine the efficacy and performance of the product in an 11 400 l dipping vat. Except for the period when heifers were dipped, animals were restrained in stanchions placed individually inside 3.3×3.3 m² stalls within an open-sided barn. The amitraz in the vat was stabilized with hydrated lime to maintain a pH of ca. 12. Analyses of vat samples showed that concentrations of amitraz in the vat were 7.6 to 13% lower than the targeted concentrations of 0.010, 0.015, 0.020, and 0.025% active ingredient (AI) for dilutions prepared according to instructions on the manufacturer's label. The large quantity of hydrated lime added to the vat (10 kg/1000 l) interfered with the HPLC analysis of vat samples. Therapeutic efficacy of each of the four observed concentrations (0.0088, 0.0131, 0.0174, and 0.0231% AI) of amitraz was excellent (>99% control). However, the rapid detachment of all ticks from an animal within a few hours after treatment with amitraz, that has been frequently observed, was not pronounced in the present study. Only 47% of the B. microplus detached in the first 4 h post-treatment, and 84% detached within the first 24 h. All of the treatments, except the lowest concentration, provided protection of cattle against re-infestation by B. microplus larvae for 14 days post-treatment. Possibly as a result of the formation of a compact layer of lime and amitraz on the bottom after the vat was undisturbed for six weeks, intense agitation was required to re-suspend the active ingredient.  相似文献   

Lunar surface rocks and fines: chemical composition     

Ahrens LH  Danchin RV 《Science (New York, N.Y.)》1970,167(3914):87-88

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Strains of avian paramyxovirus type 1 of low pathogenicity for chickens isolated from poultry and wild birds in Denmark     

Jørgensen PH  Handberg KJ  Ahrens P  Therkildsen OR  Manvell RJ  Alexander DJ 《The Veterinary record》2004,154(16):497-500

Twenty-one strains of avian paramyxovirus type 1 of low virulence for chickens were isolated in Denmark between 1996 and the beginning of 2003. The low virulence of the strains was demonstrated by sequencing the fusion (F) gene at the cleavage site motif and in some cases by determining the intracerebral pathogenicity index in day-old chicks. By using a panel of monoclonal antibodies it was shown that the isolates belonged to four different antigenic groups (five C2 isolates, six E isolates, six H isolates and four G/Q isolates). They were placed in three distinguishable genetic groups by phylogenetic analysis of a partial sequence of the F gene. The origin of the six E isolates was probably contaminated vaccines; the other viruses were isolated from wild birds and from poultry which probably came into contact with wild birds.  相似文献   

Investigation of the correlation of serum IL‐31 with severity of dermatitis in an experimental model of canine atopic dermatitis using beagle dogs          下载免费PDF全文

Rosanna Marsella  Kim Ahrens  Rachel Sanford 《Veterinary dermatology》2018,29(1):69-e28

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