Purification and enzymatic properties of a neutral metalloprotease produced from the cold-adapted <Emphasis Type="Italic">Vibrio</Emphasis> species Pr21 isolated from deep seawater in Sagami Bay     

Masami Ishida  Chie Yoshida-Mishima  Yosuke Maeda  Masashi Yamamoto  Ryo Tsuda  Haruto Ishii  Naoto Urano  Hiroshi Kabasawa 《Fisheries Science》2016,82(4):675-683

  相似文献   

Genetic diversity of wild soybean (<Emphasis Type="Italic">Glycine soja</Emphasis> Sieb. et Zucc.) and Japanese cultivated soybeans [<Emphasis Type="Italic">G. max</Emphasis> (L.) Merr.] based on microsatellite (SSR) analysis and the selection of a core collection     

Yosuke Kuroda  Norihiko Tomooka  Akito Kaga  S. M. S. W. Wanigadeva  Duncan A. Vaughan 《Genetic Resources and Crop Evolution》2009,56(8):1045-1055

Wild soybeans, Glycine soja, are a source of genetic variation to improve soybeans. To improve the efficiency evaluation of conserved germplasm a core or mini-core collection approach that maximizes allelic diversity in a proportion of the whole collection has frequently been advocated. The genetic diversity of a wild soybean collection (1,305 accessions) plus Japanese cultivated soybeans (53 accessions) were analyzed at 20 SSR marker loci. Higher levels of allelic diversity were found in wild soybeans (28 alleles per locus) than Japanese cultivated soybean (five alleles per locus). The genetic distance between wild soybeans from different regions reflected their proximity. Accessions from Russia consisted of a diverse array of alleles resulting in accessions being spread further apart in a PCA plot than accessions from other regions. Accessions of wild soybean from Korea included many rare alleles and thus had a high representation in the core collection. The two core collections developed here, traditional and mini, consisted of 192 accessions with 97% of the allelic diversity (14% of the whole collection) and 53 accessions with 62.4% of the allelic diversity (5% of the whole collection), respectively.  相似文献   

Effects of Whole Grain Wheat Bread on Visceral Fat Obesity in Japanese Subjects: A Randomized Double-Blind Study     

Yosuke Kikuchi  Satomi Nozaki  Miki Makita  Shoji Yokozuka  Shin-ichi Fukudome  Takashi Yanagisawa  Seiichiro Aoe 《Plant foods for human nutrition (Dordrecht, Netherlands)》2018,73(3):161-165

Metabolic syndrome is a risk factor for cardiovascular diseases and has become increasingly common in Japan. Epidemiological studies show inverse associations between intake of whole wheat grains and metabolic syndrome, but few dietary intervention trials have investigated the effect of whole wheat grain consumption. It was investigated whether a diet in which refined wheat bread (RW diet) was substituted by whole grain wheat bread (WW diet) would reduce visceral fat obesity in Japanese subjects. A randomized double-blind placebo-controlled intervention study was conducted in 50 Japanese subjects with body mass index (BMI)?≥?23 kg/m². Subjects were randomly assigned WW (WW group) or RW diets (RW group) for 12 weeks. Blood samples and computed tomography scans were obtained every 6th week. The WW group showed decrease (?4 cm²) in visceral fat area (VFA) (p <?0.05), whereas the RW group showed no significant changes. These time-dependent changes were significantly different between the groups. WW diet led to significant and safe reductions in VFA in subjects with BMI?≥?23 kg/m². WW diet may contribute to preventing visceral fat obesity.  相似文献   

Production efficiency and effect of water management on rice yield in Japan: two-stage DEA model on 110 paddy fields of a large-scale farm     

Dongpo Li  Teruaki Nanseki  Yosuke Chomei  Shuichi Yokota 《Paddy and Water Environment》2018,16(4):643-654

  相似文献   

S-genotype identification based on allele-specific PCR in Japanese pear     

Kenji Nashima  Shingo Terakami  Sogo Nishio  Miyuki Kunihisa  Chikako Nishitani  Toshihiro Saito  Toshiya Yamamoto 《Breeding Science》2015,65(3):208-215

Gametophytic self-incompatibility in Japanese pear (Pyrus pyrifolia Nakai) is controlled by the single, multi-allelic S-locus. Information about the S-genotypes is important for breeding and the selection of pollen donors for fruit production. Rapid and reliable S-genotype identification system is necessary for efficient breeding of new cultivars in Japanese pear. We designed S allele-specific PCR primer pairs for ten previously reported S-RNase alleles (S¹–S⁹ and S^k) as simple and reliable method. Specific nucleotide sequences were chosen to design the primers to amplify fragments of only the corresponding S alleles. The developed primer pairs were evaluated by using homozygous S-genotypes (S¹/S¹–S⁹/S⁹ and S^4sm/S^4sm) and 14 major Japanese pear cultivars, and found that S allele-specific primer pairs can identify S-genotypes effectively. The S allele-specific primer pairs developed in this study will be useful for efficient S-genotyping and for marker-assisted selection in Japanese pear breeding programs.  相似文献   

Oat Retrotransposon OARE-1 Is Activated in Both Compatible and Incompatible Interactions with Pathogenic Fungi     

Yosuke KIMURA  Yukio TOSA  Shigeyuki BETSUYAKU  Yukio SASABE  Reiko TOMITA  Jiro MURAKAMI  Hitoshi NAKAYASHIKI  Shigeyuki MAYAMA 《Journal of General Plant Pathology》2002,68(1):8-14

  相似文献   

Development of a method for forest cafeteria tests for evaluating chemical deterrents against bark stripping by sika deer (Cervus nippon yesoensis)     

Ken Orihashi  Yosuke Yasui  Yasuo Kojima  Minoru Terazawa  Shigehiro Kamoda  Hisatomi Kasahara  Yasuo Takahashi 《Journal of Forest Research》2002,7(1):35-40

Damage caused by sika deer (Cervus nippon yesoensis) is a serious problem in commercial and environmental (non-harvested) forests in Hokkaido, northern Japan. Cafeteria tests in forests may be useful for evaluating the efficacy of chemical deterrents against bark stripping by deer. To develop a method for forest cafeteria tests in the continuous snow cover period, two experiments were carried out. In the experiments, logs were produced from tree trunks, and used as carriers of chemical deterrents. Carriers were installed in forests and fed to deer. The first experiment was to find suitable sites and installation methods for carriers. Criteria for the local suitability and the installation methods were as follows: a) Sites where deer are active should be selected; b) Carriers should be installed along actively used deer trails; c) Installation sites of carriers should be changed in response to deer movement; d) Carriers should be produced from tree species that deer naturally prefer; and e) Each carrier should be partially buried in the snow. The second experiment evaluated the feasibility of a cafeteria test method based on the results of the first experiment. The method was used for 13 sets of the cafeteria test, in which the deterrent effectiveness of 5 chemicals (wood tar, rosin, wood vinegar, and 2 pyroligneous liquors) was examined. We obtained results from all the sets. The chemicals tested did not deter bark stripping by deer. Nevertheless, the method used in the present study was practical for the cafeteria tests.  相似文献   

Generation of transgenic hybrid aspen that express a bacterial gene for feruloyl-CoA hydratase/lyase (<Emphasis Type="Italic">FerB</Emphasis>), which is involved in lignin degradation in <Emphasis Type="Italic">Sphingomonas paucimobilis</Emphasis> SYK-6     

Shinya?KajitaEmail author  Fumiko?Honaga  Mikiko?Uesugi  Yosuke?Iimura  Eiji?Masai  Shinya?Kawai  Masao?Fukuda  Noriyuki?Morohoshi  Yoshihiro?Katayama 《Journal of Wood Science》2004,50(3):275-280

  相似文献   

Effects of feeding polyphenol‐rich winery wastes on digestibility,nitrogen utilization,ruminal fermentation,antioxidant status and oxidative stress in wethers          下载免费PDF全文

Kyohei Ishida  Yosuke Kishi  Kazato Oishi  Hiroyuki Hirooka  Hajime Kumagai 《Animal Science Journal》2015,86(3):260-269

Four wethers were used in a 4 × 4 Latin square design experiment to evaluate the availability of two types of winery wastes, winery sediment and grape pomace, as ruminant feeds possessing antioxidant activities. Each wether was assigned to one of the following four treatments: (i) 75 g/kg winery sediment (WS) on a dry matter (DM) basis; (ii) 166 g/kg DM winery grape pomace (WP); (iii) control diet (CD; 17 g/kg DM soybean meal);and (iv) only tall fescue hay (TFH; no additive). Winery sediment and grape pomace had high levels of polyphenols and of radical scavenging activities. Feeding with winery sediment and grape pomace did not negatively affect the intake, but it depressed crude protein (CP) digestibility compared with CD (P = 0.052 and P < 0.01 for WS and WP, respectively). Polyphenols in winery wastes decreased ruminal ammonia production (P = 0.089 and P < 0.05), likely due to their inhibitive effect on microbial activities in the rumen. The addition of winery sediment and grape pomace decreased urinary 8‐hydroxy‐2'‐deoxyguanosine (8‐OHdG; an index of oxidative damages) excretion per day (P < 0.05 and P = 0.059). The results indicated that winery sediment and grape pomace could alter nitrogen metabolism and/or act as new antioxidants for ruminants.  相似文献   

Effects of vertically transferred 3,3',4,4',5-pentachlorobiphenyl on gene expression in the ovaries of immature Sprague-Dawley rats     

Sakurada Y  Shirota M  Mukai M  Inoue K  Akahori F  Watanabe G  Taya K  Shirota K 《The Journal of reproduction and development》2007,53(4):937-943

We have previously shown that 3,3',4,4',5-pentachlorobiphenyl (PCB-126) vertically transferred from dams potentially exerts a direct effect on the ovaries of offspring and adversely affects female puberty. To investigate its toxicological targets in ovarian tissues, mRNAs encoding representative peptides that regulate follicular development in granulosa cells, theca cells, and oocytes were quantified using ovaries collected on postnatal days (PND) 5, 15, and 24 from the offspring of dams administered oral doses of 0, 1 or 3 microg/kg PCB-126 starting 2 weeks prior to mating and continuing until 20 days after delivery. Quantification using the real-time RT-PCR method revealed that PCB-126 lowered the amounts of mRNAs that encoded the inhibin alpha- and inhibin/activin beta A-subunits from PND 15 onwards; the amounts of mRNAs for inhibin/activin beta B-subunit, follicle-stimulating hormone (FSH) receptor, and aromatase on PND 15; and the amounts of luteinizing hormone receptor mRNA on PND 24 compared with those of the age-matched controls. In contrast, no differences were noted for mRNAs encoding c-kit, growth differentiation factor-9, bone morphogenetic protein-15, or kit ligand for any of the age groups examined. The serum FSH level on PND 24 was higher than that in the control. Since the earliest effects on the mRNAs in the rat ovaries were observed in those expressed in the granulosa cells of the growing follicles after the antral follicles had developed, molecules in granulosa cells but not in oocytes during the early stages of the antral follicles might be the primary targets of vertically transferred PCB-126.  相似文献