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71.
We investigated morphological changes in wood tissues of sugi (Cryptomeria japonica) resulting from treatment with the ionic liquid 1-ethyl-3-methylimidazolium chloride ([C2mim][Cl]), which dissolves cellulose. Treatment with [C2mim][Cl] caused dissociation and distortion of tracheids in latewood, but not in earlywood. This difference was due to the difference in swelling behavior of the cell wall between earlywood and latewood. Many pit membranes in bordered pits were broken by treatment with [C2mim][Cl]. In addition, some chemical changes in wood components, such as cellulose and lignin, occurred before significant disruption or destruction of the cell wall. Our results show that the reaction of wood liquefaction by [C2mim][Cl] treatment is not homogeneous, both from chemical and morphological viewpoints. 相似文献
72.
Nobutaka SOMEYA Masami NAKAJIMA Kazuyuki HIRAYAE Tadaaki HIBI Katsumi AKUTSU 《Journal of General Plant Pathology》2001,67(4):312-317
Serratia marcescens strain B2 is an effective biocontrol agent against gray mold of cyclamen, caused by Botrytis cinerea Persoon. Strain B2 has strong antifungal activity against B. cinerea in vitro. The culture filtrate was found to contain different types of chitinolytic enzyme activity, including endochitinase and chitobiase
activity. Four chitinolytic enzymes were detected among the extracellular proteins of strain B2. Two major enzymes, a 58-kDa
endochitinase and a 98-kDa chitobiase, were purified by chromatography and electrofocusing, respectively. Both enzymes inhibited
spore germination of B. cinerea. And a red pigment, prodigiosin, extracted and purified from the bacterial cells, also inhibited spore germination. When prodigiosin
and the chitinolytic enzymes were applied in concert, a synergistic inhibitory effect was observed. S. marcescens strain B2 has multiple modes of action against the pathogen.
Received 14 February 2001/ Accepted in revised form 31 May 2001 相似文献
73.
Masami NAKAJIMA Junko SUZUKI Takehiko HOSAKA Tadaaki HIBI Katsumi AKUTSU 《Journal of General Plant Pathology》2001,67(3):212-214
The BMR1 gene encoding an ABC transporter was cloned from Botrytis cinerea. To examine the function of BMR1 in B. cinerea, we isolated BMR1-deficient mutants after gene disruption. Disruption vector pBcDF4 was constructed by replacing the BMR1-coding region with a hygromycin B phosphotransferase gene (hph) cassette. The BMR1 disruptants had an increased sensitivity to polyoxin and iprobenfos. Polyoxin and iprobenfos, structurally unrelated compounds,
may therefore be substrates of BMR1.
Received 18 December 2000/ Accepted in revised form 18 April 2001 相似文献
74.
Kawashima C Kida K Matsuhashi M Matsui M Shimizu T Matsunaga N Ishii M Miyake Y Miyamoto A 《The Journal of reproduction and development》2008,54(1):46-51
The aim of our study was to investigate the effects of suckling on reproductive performance and metabolic status of obese (mean body condition score of more than 4.0 on a scale of 1-5) maternal Japanese Black cows during early postpartum period. We used 7 postpartum Japanese Black cattle. Four cows were suckled ad libitum (suckled) until completion of their first artificial insemination (AI), while 3 cows were not suckled at all because they were separated from their calves immediately after parturition (non-suckled). Body weight and plasma concentrations of metabolites and hormones were measured from wk 1 to 9 postpartum. Ovarian activity was detected using plasma progesterone concentration, and all cows received their first AI after application of the Ovsynch protocol at approximately 4 months postpartum. Although body weights of non-suckled cows increased during experimental period (P<0.05), those of suckled cows remained unchanged. Plasma concentrations of glucose of non-suckled cows were higher at wk 2 postpartum (P<0.05) and their levels of non-esterified fatty acid tended to be lower at wk 1 and 2 postpartum compared with suckled cows (P<0.1); however, these differences between groups were not observed with progression of postpartum period. In addition, plasma insulin concentrations of non-suckled cows were higher than those of suckled cows during experimental period (P<0.05). During sampling period (wk 0 to 9 postpartum), onset of normal ovarian cycle was observed in all non-suckled and 2 of 4 suckled cows, and it was delayed in other 2 suckled cows compared with non-suckled cows; however, 3 suckled cows conceived at the first AI after application of the Ovsynch protocol; none of non-suckled cows conceived at this time. Overall, we suggest that suckling seems to reduce increase of body weight after parturition, although it does not improve obesity, and influences conception despite delay in resumption of normal ovarian cyclicity in obese Japanese Black cows. 相似文献
75.
Masaki KATO Daiki KAWASHIMA Yui ITO Osamu YAMAMOTO Hiromi NAKAJIMA Takuya OIZUMI Kaori SHIMIZU Yasuo INOSHIMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(2):304
Nasal papules and oral ulcers were observed in calves that were group-housed at a dairy farm. The calves were diagnosed with bovine papular stomatitis (BPS) due to parapoxvirus (PPV) infection based on virologic examinations using polymerase chain reaction to detect PPV. To prevent the spread of BPS, we isolated the affected calves, made procedural changes so that the affected herd was managed after the healthy herd, disinfected the bedding with slaked lime, disinfected the stalls and fences with invert soap, and changed the animals’ feed to soft grass which does not damage the oral cavity. As a result, we succeeded in control the infection quickly. 相似文献
76.
Sybuia Priscila Ayumi de Castro-Prado Gabriela de Carvalho Nunes William Mário Zanutto Carlos Alexandre Kaneshima Edilson Nobuyoshi Soares Dartanhã José da Silva Franco Claudinéia Conationi de Freitas Mathias Paulo Cezar de Castro-Prado Marialba Avezum Alves 《European journal of plant pathology / European Foundation for Plant Pathology》2022,163(4):937-950
European Journal of Plant Pathology - Charcoal rot is an economically important fungal disease of economically important crops. Several species of the Macrophomina genus, such as M.... 相似文献