Molecular detection of Bartonella henselae and Bartonella clarridgeiae in clinical samples of pet cats from Southern Italy     

M.G. Pennisi  E. La Camera  L. Giacobbe  B.M. Orlandella  V. Lentini  S. Zummo  M.T. Fera 《Research in veterinary science》2010,88(3):379-384

Bartonella henselae is considered an emerging pathogen of veterinary and medical interest that can be occasionally transmitted to humans. Cats are considered to be the only reservoir host for B. henselae. In this study, we used a nested-PCR assay to investigate the prevalence of B. henselae and Bartonella clarridgeiae DNA in peripheral blood samples, fine needle lymph node aspirate specimens and oral swabs from 85 cats in order to develop an easy diagnostic strategy for the selection of infection-free cats that are being considered as pets, especially for immunocompromised patients. Overall, molecular analysis showed that 71 cats (83.5%) tested PCR positive for the presence of B. henselae DNA. PCR amplification of DNA B. henselae produced positive products from lymph node aspirate specimens (62/85; 72.9%) similar to those obtained from blood samples (60/85; 70.6%) and higher than those from oral swabs (51/85; 60%) of cats. No PCR product was obtained for B. clarridgeiae. The simultaneous analysis of three different clinical samples in our study increased the diagnostic possibilities for B. henselae infection in the examined cats from 60–72.9% to 83.5%. Lymph node aspirates were found to be the most effective clinical samples for the detection of B. henselae and blood samples were the next best. Oral swab samples were used in this study with good results when considered in combination with blood and/or lymph node aspiration. The use of nested-PCR assay on these three clinical samples may enhance the diagnostic sensitivity for bartonellosis in cats irrespective of the clinical status of animals.  相似文献   

Retraction     

Beloqui A  Guazzaroni ME  Pazos F  Vieites JM  Godoy M  Golyshina OV  Chernikova TN  Waliczek A  Silva-Rocha R  Al-Ramahi Y  La Cono V  Mendez C  Salas JA  Solano R  Yakimov MM  Timmis KN  Golyshin PN  Ferrer M 《Science (New York, N.Y.)》2010,330(6006):912

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利用GnRH+PGF2α进行绵羊同期发情的效果观察     

杨梅  汪立琴  陈童  郭志勤  郝根  莎拉  郭立 《黑龙江动物繁殖》2010,18(2):14-15

利用99只绵羊,在繁殖季节,采用促排卵3号+PG诱导同期发情的试验。结果表明,72h内的发情率达100%,排卵率在90%以上;对86只受体羊的黄体侧子宫角移植两枚经玻璃化冷冻-解冻后的胚胎,其总受胎率为38.37%。  相似文献   

青海高原日光温室秋冬茬西芹种植技术     

喇成寿 《农业科技通讯》2010,(8):194-195

西芹与我县传统种植的本地芹相比,具有药味淡、纤维含量低、脆嫩、耐寒、适应性强、产量高等特点,而且生长旺盛、叶柄变大、营养丰实,有降压、健脑、清肠、利便的作用。  相似文献   

Aporphine alkaloids,clerodane diterpenes,and other constituents from Tinospora cordifolia     

Phan Van Kiem  Chau Van Minh  Nguyen Tien Dat  La Van Kinh  Dan Thuy Hang  Nguyen Hoai Nam  Nguyen Xuan Cuong  Hoang Thanh Huong  Trinh Van Lau 《Fitoterapia》2010

Phytochemical investigation of the methanol extract of Tinospora cordifolia aerial parts led to the isolation of four new and seven known compounds. The structures of two new aporphine alkaloids, N-formylasimilobine 2-O-β-d-glucopyranosyl-(1 → 2)-β-d-glucopyranoside (tinoscorside A, 1) and N-acetylasimilobine 2-O-β-d-glucopyranosyl-(1 → 2)-β-d-glucopyranoside (tinoscorside B, 2), a new clerodane diterpene, tinoscorside C (3), and a new phenylpropanoid, sinapyl 4-O-β-d-apiofuranosyl-(1 → 6)-O-β-d-glucopyranoside (tinoscorside D, 6) were determined by extensive spectroscopic methods including FTICR-MS and 1D and 2D NMR.  相似文献   

Using plant essential oils to control Fusarium wilt in tomato plants     

A. La Torre  F. Caradonia  A. Matere  V. Battaglia 《European journal of plant pathology / European Foundation for Plant Pathology》2016,144(3):487-496

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Low salinity increases survival,body weight and development in tadpoles of the Chinese edible frog Hoplobatrachus rugulosus          下载免费PDF全文

La‐iad Nakkrasae  Sukpaporn Phummisutthigoon  Narattaphol Charoenphandhu 《Aquaculture Research》2016,47(10):3109-3118

Water salinity affects survival, growth and metamorphosis of anuran tadpoles. Hoplobatrachus rugulosus is considered not only as a freshwater amphibian but is also found in brackish wetlands. However, whether salinity change interferes with hatching, survival, body mass and development of H. rugulosus tadpoles is unknown. We found that salinity levels of <4‰ did not affect of survival or hatching of H. rugulosus eggs. At an early larval stage, tadpoles could tolerate up to 9‰ salinity for 96 h; however, body water content decreased when salinity was >5‰. After a 3‐week experiment, body weights of tadpoles exposed to 2‰ and 4‰ salinities were higher but that of the 6‰ group was lower compared with the 0‰ group. More than 90% of tadpoles exposed to 2‰ and 4‰ salinity showed complete metamorphosis. Salinity levels <4‰ promoted survival of tadpoles better than 0‰, whereas none of tadpoles in the 6‰ group became juvenile frogs in 50 days. Time taken to reach metamorphosis was shorter for 2‰ and 4‰ (47.22 ± 0.28 and 47.26 ± 0.33 days, respectively) than for 0‰ (49.31 ± 0.35 days). Juvenile frogs in the 2‰ group had greater body weight than the control. It could be concluded that salinity of <4‰ increased survival and body weight of H. rugulosus tadpoles, and shortened the time taken to reach metamorphosis.  相似文献   

Allium vegetables intake and endometrial cancer risk     

Galeone C  Pelucchi C  Dal Maso L  Negri E  Montella M  Zucchetto A  Talamini R  La Vecchia C 《Public health nutrition》2009,12(9):1576-1579

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杂交相思组织培养研究初报   总被引：1，自引：0，他引：1  

周传明  梁机  罗军明  吕曼芳  黄超群 《广西农业科学》2013,(7):1165-1168

【目的】筛选杂交相思组织培养不同培养阶段的培养基配方,为实现杂交相思工厂化育苗提供技术参考。【方法】用杂交相思嫩茎为外植体,探究生长调节剂6-BA、NAA、IBA对其诱导出芽、继代增殖和诱导生根的效果。【结果】在不添加6-BA的情况下,添加0.2mg/LNAA时芽诱导率为60.0%,当添加0.2mg/L的6-BA后,诱导率为42.0%。当NAA为0~0.5mg/L时,随着6-BA的增加,增殖系数随之增加,6-BA为0.8mg/L时,增殖系数为4.2;当6-BA为1.0mg/L以上时,继代苗的增殖系数为1.0。以改良MS为基本培养基,在NAA1.0mg/L的条件下添加0.5、1.0mg/L的IBA生根诱导率分别为17.5%、24.0%;以改良1/2MS为基本培养基,在NAA1.0mg/L的条件下,添加0.5mg/L的IBA生根率为91.7%,当添加IBA为1.0mg/L时,生根率降到50.0%;生根苗移栽基质以黄心土与河沙或蛭石混合较好,移栽成活率在85.0%以上。【结论】改良MS＋NAA0.2mg/L培养基是较适宜芽诱导的培养基;改良MS＋6-BA0.8mg/L＋NAA0.5mg/L培养基较利于继代苗的生长;改良1/2MS＋NAA1.0mg/L＋IBA0.5mg/L培养基较改良MS培养基有利于诱导生根。  相似文献   

The frost hardiness of some clones of olive cv. Leccino     

N. La Porta  M. Zacchini  S. Bartolini  R. Viti  G. Roselli 《The Journal of Horticultural Science and Biotechnology》2013,88(3):433-435

Summary

Frost hardiness of four olive clones (Olea europaea L.) belonging to cv. Leccino was studied and compared with that of a susceptible clone of cv. Moraiolo by ionic leakage. Variability of the trait was found within the clones of ‘Leccino’, some of which were statistically different from a the ‘Moraiolo’ clone at one or both temperatures tested. Only one clone showed a significant difference between ?15° and ?20°C. The ionic leakage technique is appropriate for studying changes in physical properties of membranes and its use in the screening of frost tolerant olive clones is discussed.  相似文献