The effect of a high dose bovine herpes virus 1 marker vaccine in pregnant heifers: virological, bacteriological, immunological, and pathological findings     

Bruschke CJ  Kamp EM  Boersma W  Stockhofe-Zurwieden N  Bouma A 《Tijdschrift voor diergeneeskunde》2001,126(6):211-217

To determine a possible relationship between the compulsory vaccination against bovine herpesvirus 1 (BHV1) and cattle wasting disease, the effects of BHV1 vaccination on heifers were investigated. Twenty heifers in the third trimester of pregnancy were randomly allotted to a vaccine and a control group. The vaccine group was vaccinated twice with a 50-fold dose of BHV1 vaccine and the control group was inoculated with the diluent. The experiment was performed double blind. After vaccination, the cows were examined daily and condition scores were determined weekly. Blood, milk, and faeces samples were collected weekly for virological, bacteriological, and immunological investigation. The heifers were euthanized either 9 or 13 weeks after the first inoculation and pathological, virological, and bacteriological examination was performed. No differences were detected between the vaccine group and the control group. No concurrent infections were detected and there were no indications of immunosuppression after vaccination. No relationship between the BHV1 vaccination and wasting disease in cattle was detected.  相似文献   

Efficacy of a combination of 10% imidacloprid/50% permethrin for the prevention of leishmaniasis in kennelled dogs in an endemic area     

Otranto D  Paradies P  Lia RP  Latrofa MS  Testini G  Cantacessi C  Mencke N  Galli G  Capelli G  Stanneck D 《Veterinary parasitology》2007,144(3-4):270-278

The efficacy of imidacloprid 10%and permethrin 50% (Advantix; Bayer AG, Germany) in a spot-on formulation was evaluated in the field as a control measure to prevent canine leishmaniasis (CanL) in dogs in an endemic area of southern Italy. In February 2005, out of 845 dogs initially tested for CanL, 631 dogs which tested negative (315 from a kennel in Bari (KB) and 316 from a kennel in Ginosa (KG)) in a serological and a parasitological examination were allocated to one of three groups: Group A-treated with imidacloprid 10% and permethrin 50% once a month; Group B-treated every 2 weeks; and Group C-untreated control animals. All the dogs were examined serologically and parasitologically for CanL prior to the start of the study, in November 2005 (end of the sandfly season) and in March 2006 (end of the study). An initial CanL seroprevalence of 24.7% (209 dogs) was detected in KB and KG. In KB Leishmania infection, inferred by positivity in at least one of the three tests performed at the interim or final follow-up, was found in one animal from Group A and in nine from Group C. No positive animals were detected in Group B, thus giving a final protection efficacy of 88.9% in Group A and 100% in Group B. In KG Leishmania infection was identified in one animal from Groups A and B, respectively, and 11 from Group C (protection efficacy of 90.36% in Group A and 90.73% in Group B). The incidence density rates (IDRs) of infection in both Groups A and B at each kennel were statistically significantly lower than that registered in Group C (KB p<0.05 and KG p<0.01). The results clearly show that a combination of imidacloprid 10% and permethrin 50%, by virtue of its repellent activity against sandflies, is effective under both application regimes in preventing CanL in the field in endemic areas.  相似文献   

Granulomatous lesions in lymph nodes of slaughter pigs bacteriologically negative for Mycobacterium avium subsp. avium and positive for Rhodococcus equi     

Komijn RE  Wisselink HJ  Rijsman VM  Stockhofe-Zurwieden N  Bakker D  van Zijderveld FG  Eger T  Wagenaar JA  Putirulan FF  Urlings BA 《Veterinary microbiology》2007,120(3-4):352-357

The prevalence of granulomatous lesions in lymph nodes of pigs was studied. From January till August 2004 in two slaughterhouses in The Netherlands 2,116,536 pigs were examined for the presence of granulomatous lesions in the sub-maxillary lymph nodes. In 15,900 (0.75%) of these pigs, lesions could be detected. Nine farms with the highest incidence of lesions were selected for a more detailed pathological and bacteriological examination. On these farms, the prevalence of lesions in sub-maxillary lymph nodes ranged from 2.3 to 5.7% with a mean of 3.0%. From 1276 pigs that were sampled, 98 (7.7%) displayed granulomatous lesions in the sub-maxillary lymph nodes and one (0.1%) pig showed lesions in its mesenteric lymph node. Mycobacterium avium subsp. avium (MAA) could not be isolated from the lymph nodes of the 99 pigs with lesions and from a selection of lymph nodes (n = 61) of pigs without lesions. Rhodococcus equi was isolated from 44 out of 98 (44.9%) of the sub-maxillary lymph nodes with granulomatous lesions and from two mesenteric lymph nodes without lesions. A comparison of former studies and the current results indicate that the prevalence of MAA infections in slaughter pigs has strongly decreased over the last decade, whereas R. equi is highly prevalent. The high incidence of granulomatous lesions associated with the bacteriological presence of R. equi could be considered as a serious cause of misdiagnosis of MAA infections in cases where meat inspection is carried out by inspection for granulomatous changes of lymph nodes only.  相似文献   

Cadmium fractions in an acid sandy soil and Cd in soil solution as affected by plant growth          下载免费PDF全文

Christos Stritsis  Bernd Steingrobe  Norbert Claassen 《植物养料与土壤学杂志》2014,177(3):431-437

In a previous experiment, plants were able to immobilize or solubilize Cadmium (Cd) in a sandy acid soil enriched with 40 μmol Cd kg^–1, because Cd solution concentration was decreased by maize (Zea mays) and sunflower (Helianthus annuus), and increased by flax (Linum usitatissimum L. ssp. usitatissimum) and spinach (Spinacia oleracea). It is assumed that the equilibrium with Cd fractions in the soil solid phase and the chemical form of Cd in the soil solution were affected. In the present study, the effect of the four plant species mentioned above on Cd binding in soil was investigated by means of a fractionation of soil Cd with a sequential extraction of seven steps. The seven fractions of Cd are operationally defined by the extraction sequence that follows the order of increasing acidity with extractants of different complexing and redox properties. In the unplanted soil, Cd was predominantly present in the exchangeable Fraction I (F. I) and easily mobilizable Fraction II (F. II) (64%). Significant concentrations of Cd were found in F. III (occluded in Mn oxides; 22%) and F. IV (organically bound; 10%). Fractions V (occluded in poorly crystalline Fe oxides), F. VI (occluded in well crystallized Fe oxides), and F. VII (residual fraction) amounted to less than 5% of the total soil Cd concentration. The plants changed the binding of Cd in soil in a different manner. All plants decreased F. I, but F. II was increased by maize and spinach, decreased by flax or remained unaffected by sunflower. Fraction III was not affected by maize and flax, but decreased by sunflower and spinach, and F. IV was not affected by sunflower and spinach, but was increased by maize and flax. These changes of Cd fractions were not related to the changes the plants had caused in total Cd or Cd²⁺ concentration of the soil solution. These results show that plant species differ in how they affect Cd binding to the soil solid phase, but this effect is not related to how they affect Cd in soil solution. The mechanisms by which plants affect the relationship between the soil solid and liquid phase are still unclear.  相似文献   

The incorporation of extreme drought events improves models for beech persistence at its distribution limit     

Ervin Rasztovits  Imre Berki  Csaba Mátyás  Kornél Czimber  Elisabeth Pötzelsberger  Norbert Móricz 《Annals of Forest Science》2014,71(2):201-210

Context

Projections of species distribution models under future climate are usually based on long-term averages. However, singular extreme drought events presumably contribute to the shaping of distribution limits at the retreating low-elevation xeric limits.

Methods

The objectives of this study were to set up a distribution model based on extreme drought events (EDM), which uses sanitary logging information as a proxy of vitality response of beech, and compare it with the results of classical species distribution models (SDMs).

Results

Predictions of the EDM for 2025 were in agreement with those of the SDM, but EDM predicted a more serious decline in all regions of Hungary towards the end of the century.

Conclusion

These results suggest that the predicted increase in frequency and severity of drought events may further limit the distribution of beech in the future.  相似文献   

Control of MicroRNA Expression as a New Way for Resveratrol To Deliver Its Beneficial Effects     

Lançon A  Kaminski J  Tili E  Michaille JJ  Latruffe N 《Journal of agricultural and food chemistry》2012,60(36):8783-8789

Grapes produce large amounts of polyphenols. Many of them accumulate in the skin, pulp, and seeds and are consequently found in wine. The health benefits of a moderate consumption of wine have been attributed at least in part to grape's polyphenols. Among them, resveratrol (3,5,4'-trihydroxystilbene) is a phytoalexin that stimulates plant cell defenses against infections and also plays protective roles in humans, where it delays cardiovascular alterations and exerts anticancer and anti-inflammatory effects. Despite numerous studies, the molecular mechanisms of resveratrol action are only partially understood. Given its pleiotropic effects, it was previously suggested that resveratrol protective properties may arise from its modulation of the expression of microRNAs. Therefore, this review will focus on the effects of resveratrol on microRNA populations in humans and human cell lines, especially emphasizing the microRNAs that have been implicated in resveratrol effects on inflammation, cancer, metabolism, and muscle differentiation.  相似文献   

Science education. Educating future scientists     

Sung NS  Gordon JI  Rose GD  Getzoff ED  Kron SJ  Mumford D  Onuchic JN  Scherer NF  Sumners DL  Kopell NJ 《Science (New York, N.Y.)》2003,301(5639):1485

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Extrusion and death of DPP/BMP-compromised epithelial cells in the developing Drosophila wing     

Gibson MC  Perrimon N 《Science (New York, N.Y.)》2005,307(5716):1785-1789

During animal development, epithelial cell fates are specified according to spatial position by extracellular signaling pathways. Among these, the transforming growth factor beta/bone morphogenetic protein (TGF-beta/BMP) pathways are evolutionarily conserved and play crucial roles in the development and homeostasis of a wide range of multicellular tissues. Here we show that in the developing Drosophila wing imaginal epithelium, cell clones deprived of the BMP-like ligand Decapentaplegic (DPP) do not die as previously thought but rather extrude from the cell layer as viable cysts exhibiting marked abnormalities in cell shape and cytoskeletal organization. We propose that in addition to assigning cell fates, a crucial developmental function of DPP/BMP signaling is the position-specific control of epithelial architecture.  相似文献   

Genome-wide RNAi analysis of growth and viability in Drosophila cells     

Boutros M  Kiger AA  Armknecht S  Kerr K  Hild M  Koch B  Haas SA  Paro R  Perrimon N;Heidelberg Fly Array Consortium 《Science (New York, N.Y.)》2004,303(5659):832-835

A crucial aim upon completion of whole genome sequences is the functional analysis of all predicted genes. We have applied a high-throughput RNA-interference (RNAi) screen of 19,470 double-stranded (ds) RNAs in cultured cells to characterize the function of nearly all (91%) predicted Drosophila genes in cell growth and viability. We found 438 dsRNAs that identified essential genes, among which 80% lacked mutant alleles. A quantitative assay of cell number was applied to identify genes of known and uncharacterized functions. In particular, we demonstrate a role for the homolog of a mammalian acute myeloid leukemia gene (AML1) in cell survival. Such a systematic screen for cell phenotypes, such as cell viability, can thus be effective in characterizing functionally related genes on a genome-wide scale.  相似文献   

Endemic papillomavirus infection of roe deer (Capreolus capreolus)     

Kroly Erdlyi  Lszl Dencs&#x;  Rbert Lehoczki  Mikls Heltai  Krisztina Sonkoly  Sndor Csnyi  Norbert Solymosi 《Veterinary microbiology》2009,138(1-2):20-26

Roe deer papillomavirus (CcPV1) infection has been identified as an endemic disease in roe deer populations of the Carpathian basin in Central Europe (Hungary, Austria and Croatia). The disease is characterised by easily recognizable skin tumours similar to deer papillomavirus infection of North American deer species. In 2006, a questionnaire study was conducted among all Hungarian game management units (GMUs) in order to assess the distribution of the disease and its major epidemiological features. Categorical information was collected about disease occurrence, trend and frequency of detection, on primarily affected age classes in both sexes, and association of lesions with mortality. Replies were received from 539 GMUs representing 50.9% of total GMU territory and disease presence was reported by 295 (54.7%) GMUs. Older age classes of both sexes were found to be more affected. Association of various environmental factors with disease occurrence was evaluated and data were collected on the occurrence of similar skin lesions in other European countries. Pathological features of CcPV1 infection were described and the localisation of both CcPV1 antigen and DNA was characterised by immunohistochemistry and in situ DNA hybridisation in skin lesions. Virus presence was also demonstrated by PCR and PCR product sequencing.  相似文献