Isolation of ureaplasmas from poultry and experimental infection in chickens     

K Koshimizu  H Kotani  T Magaribuchi  T Yagihashi  K Shibata  M Ogata 《The Veterinary record》1982,110(18):426-429

Ureaplasmas were isolated from the oropharynxes of 47 of 247 (19 per cent) Leghorn chickens (Gallus gallus domesticus) and from five Japanese bantams but none was isolated from the oropharynx or cloaca of other poultry comprising 10 Japanese game, 75 common quails (Coturnix coturnix japonica), 17 turkeys (Meleagris gallopavo) and 10 guinea fowls (Numida galeata). In apparently healthy chickens, ureaplasmas were found at various sites, including the conjunctiva, nasal cavity, oropharynx, upper and lower tracheas, but not from the air sac, lungs, yolk, oviduct, urine or cloaca. All the isolates were antigenically similar but had no serological relation to those isolated from man, monkey, cattle, goat, sheep, dog and cat. In chickens experimentally infected with an avian ureaplasma, the organisms infected the oropharynx and nasal cavity but none of the birds inoculated demonstrated any clinical signs or macroscopic lesions.  相似文献   

Notoedric mange in free-ranging masked palm civets (Paguma larvata) in Japan     

Ninomiya H  Ogata M  Makino T 《Veterinary dermatology》2003,14(6):339-344

Fifty-one masked palm civets (Paguma larvata) were trapped as part of a nuisance wildlife control programme between July 2001 and August 2002 in Fujisawa City, Kanagawa Prefecture, Japan. Eleven civets had characteristic mange lesions with marked alopecia and crusts, caused by the burrowing epidermal mite Notoedres cati. The diagnosis was confirmed by histology and examination of mites obtained from skin scrapings of affected civets. Histopathology of lesions demonstrated moderate hyperplastic epidermis, parakeratosis and acanthosis. Occasionally microvascular angiogenesis was observed in the epidermis. Tunnels were excavated in the hair follicles, reaching the hair roots. The tunnels were located between the hair shaft and the inner root sheath. Female mites, eggs and nymphs were demonstrated in their tunnels using scanning electron microscopy.  相似文献   

Studies on cytopathogenic viruses isolated from cats with respiratory infections. I   总被引：1，自引：0，他引：1  

M Kamizono  S Konishi  M Ogata  S Kobori 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1968,30(4):197-206

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Thermophilic campylobacters in seagulls and DNA-DNA hybridization test of isolates   总被引：1，自引：0，他引：1  

C Kaneuchi  T Imaizumi  Y Sugiyama  Y Kosako  M Seki  T Itoh  M Ogata 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1987,49(5):787-794

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Characterization and genetic analysis of laboratory mutants of <Emphasis Type="Italic">Cochliobolus heterostrophus</Emphasis> resistant to dicarboximide and phenylpyrrole fungicides     

Akira Yoshimi  Junko Imanishi  Abdul Gafur  Chihiro Tanaka  Mitsuya Tsuda 《Journal of General Plant Pathology》2003,69(2):101-108

 Laboratory mutants of Cochliobolus heterostrophus resistant to iprodione were obtained after chemical mutageneses. All the mutants were able to grow on the medium amended with iprodione 100 μg/ml. They showed positive cross-resistance to procymidone and fludioxonil and were sensitive to high osmolarity. Crosses between the mutant and a wild-type strain revealed that the fungicide resistance and osmotic sensitivity traits were inherited by their offspring in a 1 : 1 mutant/wild type ratio, indicating that the mutant phenotypes in these strains were due to alteration at a single gene locus. Results from allelism tests indicated that three genes (Dic1, Dic2, Dic3) conferred the mutant phenotypes. Among them, Dic1 mutant strains were classified into three types on the basis of their phenotypes. The first type was moderately resistant to the fungicides and less sensitive to osmotic stress than the other Dic1 mutant strains. The second type showed moderate fungicide resistance, but growth was inhibited under lower osmotic stress (50 mM KCl). The other Dic1 mutant strains grew well on medium containing iprodione and fludioxonil even at a concentration of 100 μg/ml and were highly sensitive to osmotic stress. The Dic2 and Dic3 mutant strains had moderate resistance to the fungicides with low-level osmotic sensitivity. The Dic1 gene was epistatic to Dic2 and Dic3 for fungicide resistance and hypostatic to them for osmotic sensitivity. These results suggest that the osmoregulatory system is involved in fungicide resistance in laboratory mutants of C. heterostrophus. Received: March 14, 2002 / Accepted: August 13, 2002  相似文献   

New sero-types of Mycoplasma laidlawii isolated from mastitic milk and urogenital tracts of cattle. I. Isolation of M. laidlawii   总被引：2，自引：0，他引：2  

I J Pan  M Ogata 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1969,31(2):83-93

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Mycoplasmas isolated from dogs with malignant lymphoma     

K Koshimizu  K Yamamoto  M Ogata 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1973,35(2):123-132

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Root biomass and distribution of a <Emphasis Type="Italic">Picea–Abies</Emphasis> stand and a <Emphasis Type="Italic">Larix–Betula</Emphasis> stand in pumiceous Entisols in Japan     

Yoshimi Sakai  Masamichi Takahashi  Nagaharu Tanaka 《Journal of Forest Research》2007,12(2):120-125

Root biomass and root distribution were studied in Entisols derived from the thick deposition of volcanic pumice on Hokkaido Island, Japan, to examine the effect of soil conditions on tree root development. The soil had a thin (<10 cm) A horizon and thick coarse pumiceous gravel layers with low levels of available nutrients and water. Two stands were studied: a Picea glehnii–Abies sachalinensis stand (PA stand) and a Larix kaempferi–Betula platyphylla var. japonica stand (LB stand). The allometric relationships between diameter at breast height (DBH) and aboveground and belowground biomass of these species were obtained to estimate stand biomass. The belowground biomass was small: 30.6 Mg ha₋₁ for the PA stand and 24.3 Mg ha₋₁ for the LB stand. The trunk/root ratios of study stands were 4.8 for the PA stand and 4.3 for the LB stand, which were higher than those from previous studies in boreal and temperate forests. All species developed shallow root systems, and fine roots were spread densely in the shallow A horizon, suggesting that physical obstruction by the pumiceous layers and their low levels of available water and nutrients restricted downward root elongation. The high trunk/root ratios of the trees may also have resulted from the limited available rooting space in the study sites.  相似文献   

HPLC quantification of kaempferol-3-O-gentiobioside in Cassia alata     

Moriyama H  Iizuka T  Nagai M  Murata Y 《Fitoterapia》2003,74(5):425-430

Kaempferol-3-O-gentiobioside, the major flavonoid glycoside in Indonesian Cassia alata was quantified in various parts of the plant. The mature leaf was found to contain the highest content of this metabolite. A decrease of the flavonoid content in the juvenile leaf during the period of October through December was also observed. The contents ranged from 2.0 to 5.0% and 1.0 to 4.0% in mature and juvenile leaves, respectively. The other parts studied were flower (sepal and petal), rachis, stem and seed. Kaempferol-3-O-gentiobioside was not detected in the seed.  相似文献   

Identification of bacteria in total mixed ration silage produced with and without crop silage as an ingredient          下载免费PDF全文

Naoki Nishino  Yu Ogata  Hongyan Han  Yasunari Yamamoto 《Animal Science Journal》2015,86(1):45-50

As a forage source for total mixed ration (TMR) silage production, locally produced crop silage is now used in addition to imported hay. This type of TMR ensiling is regarded as a two‐step fermentation process; hence, a survey was carried out to determine whether the bacteria in crop silage affect the subsequent TMR ensiling. Fermentation product contents and bacterial community were determined for TMR silage and its ingredient silages collected in August, October and November. August product contained corn, sorghum and Italian ryegrass silages, October product had wheat silage exclusively and November product did not include any crop silages. Acetic acid, lactic acid, 2,3‐butanediol and ethanol were predominant fermentation products in corn, sorghum, Italian ryegrass and wheat silages, respectively. Robust lactic acid fermentation was seen in TMR silage, even if acetate‐type and alcohol‐type silages were mixed as ingredients. The finding that bacterial community of the TMR silage appeared unrelated to those of ingredient silage supported this. Silages of various fermentation types can therefore be formulated without interfering with lactate‐type fermentation in TMR silage.  相似文献