Identification of stable and oestrus cycle-independent housekeeping genes in the rat mammary gland and other tissues     

Hvid H  Ekstrøm CT  Vienberg S  Oleksiewicz MB  Klopfleisch R 《Veterinary journal (London, England : 1997)》2011,190(1):103-108

The function and development of the rat mammary gland is dependent on the oestrus cycle. Normalization of gene expression in mammary gland samples assessed by quantitative RT-PCR therefore requires housekeeping genes (HKGs) which are stably expressed during the oestrus cycle. mRNA expression of 10 HKGs was measured in the rat mammary gland at different phases of the oestrus cycle. In addition, mRNA expression of the HKGs was measured in a panel of other rat tissues comprising laser microdissected mammary gland alveolar lobules and interlobular connective tissue and macrodissected mammary gland, liver, skeletal muscle, colon and ovary samples. Expression and ranking of HKGs varied between tissues and oestrus cycle phases and several HKGs were necessary for normalization between samples. In the mammary gland samples, three HKGs (Sdha, Tbp, and Atp5b) were identified as the optimal combination of stably expressed genes across oestrus cycle phases. For normalization between samples from the entire panel of rat tissues, eight HKGs (Rps18, Eef1a1, B2m, Actb, Tbp, Hprt, Pgk1, and Sdha) were identified as the optimal combination. These HKGs are of general relevance for studies comparing gene expression between different rat tissues.  相似文献   

IDENTIFICATION OF ARACHNOID CYSTS IN THE QUADRIGEMINAL CISTERN USING ULTRASONOGRAPHY     

Miyoko  Saito  DVM  Natasha J.  Olby  VET MB  PhD  Kathy  Spaulding  DVM 《Veterinary radiology & ultrasound》2001,42(5):435-439

The ultrasonographic findings in three small breed dogs with intracranial arachnoid cysts are described. Ultrasound images were obtained via the foramen magnum, temporal window and persistent bregmatic fontanelle (when possible). In transverse, dorsal and sagittal transcranial ultrasound images there was marked dilation of the lateral ventricles and a well-defined, oval to triangular-shaped anechoic area between the caudal aspect of the occipital lobes, dorsal to the midbrain, and rostral to the cerebellum. These findings were consistent with a diagnosis of concurrent hydrocephalus and an arachnoid cyst within the quadrigeminal cistern.  相似文献   

Endogenous Benzodiazepine Activity in the Peripheral and Portal Blood of Dogs With Congenital Portosystemic Shunts     

LILLIAN R. ARONSON VMD    REYNALDO C. GACAD MD    KRISTINE KAMINSKY-RUSS MS    CLARE R. GREGORY DVM  Dipiomate ACVS  KEVIN D. MULLEN MB  FRCPI 《Veterinary surgery : VS》1997,26(3):189-194

Objective- The purpose of this study was to determine whether an endogenous benzodiazepine receptor ligand (EBZ) was present in the arterial and portal blood of dogs with congenital portosystemic shunts (CPSS).
Study Design- The presence or absence of an EBZ was determined by the collection of systemic and portal blood from dogs with CPSS.
Animals- Fifteen client-owned dogs with a confirmed CPSS. All dogs had historical signs compatible with hepatic encephalopathy. Eight healthy dogs were used as controls.
Methods- In all dogs, systemic blood samples were collected after they were anesthetized. Portal blood samples were collected intraoperatively. EBZ was measured by radioreceptor assay.
Results- In 10 of 15 dogs, the portal blood concentration of EBZ was significantly elevated compared with normal dogs (mean, 13.2 ±18.55 ng/mL). Five dogs had elevated systemic blood EBZ levels (mean, 8.2 ±16.08 ng/mL). Eleven of 15 dogs had a higher portal than systemic blood concentration of EBZ. In contrast, control dogs had extremely low EBZ concentrations detected in their portal blood (mean, 0.16 ±0.3 ng/mL) and systemic blood (0 ng/mL). The mean portal and systemic blood concentrations in dogs with CPSS were significantly greater than in control dogs ( P <.05).
Conclusions- Elevated blood levels of EBZ were found in dogs with CPSS. The portosystemic gradient noted in 11 dogs suggests the gastrointestinal tract as a possible source for the endogenous ligand.
Clinical Relevance- Generalized motor seizures have been reported in dogs after surgical correction of CPSS. If the presence of a CPSS results in stimulation of brain receptors for benzodiazepines, post-CPSS ligation seizures may result from a withdrawal of EBZ after ligation of the portosystemic shunt.  相似文献   

TRUE DIAPHRAGMATIC HERNIA IN A CAT     

Andra K.  Voges  DVM  Scott  Bertrand  DVM  Richard C.  Hill  MA Vet MB  PhD  Lisa  Neuwirth  DVM  MS  Michael  Schaer  DVM 《Veterinary radiology & ultrasound》1997,38(2):116-119

A report of a cat with a true diaphragmatic hernia in which only falciform fat had herniated is pressented. The lession was misinterpreted as a pulmonary mass. Additional radiographic studies which may have been of diagnostic benefit are briefly discussed.  相似文献   

Normal and abnormal sex, chromosomes and H-Y antigen     

C.R.E. Hainan MA VET. MB  PhD  FRCVS 《Journal of Equine Veterinary Science》1985,5(1):38-39

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Modelling the potential impact of exotic diseases on regional Australia     

MG GARNER  MB LACK 《Australian veterinary journal》1995,72(3):81-87

SUMMARY: Recent international initiatives for disease control suggest that, in the future, the consequences for trade of an exotic disease outbreak may not be as severe as estimated in the past. If zoning were to be accepted by Australia's trading partners, then the major effects may be felt at the regional rather than the national level. A study, using an integrated epidemiological/economic model, was undertaken to compare the impacts of 3 important exotic diseases (foot-and-mouth disease, classical swine fever and sheep pox) in 3 different regions of Australia. The study demonstrated that there are significant differences between the size and effect of different disease outbreaks. Regional factors influence not only the way that the disease will spread and manifest itself, but also the effects on local communities. Foot-and-mouth disease caused more economic losses than sheep pox or classical swine fever. The major determinant of differences in the effects of the diseases between regions was the nature of the regional economies. The less diversified the economy, the greater the effect of an exotic disease outbreak in relation to the size of that economy.  相似文献   

Cryptococcal meningitis in an alpaca   总被引：1，自引：0，他引：1  

LM GOODCHILD  AJ DART  MB COLLINS  CM DART  JL HODGSON  DR HODGSON 《Australian veterinary journal》1996,74(6):428-430

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Biomechanical Comparison Between Bicortical Pin and Monocortical Screw/Polymethylmethacrylate Constructs in the Cadaveric Canine Cervical Vertebral Column     

Bianca F. Hettlich Dr. med vet  Diplomate ACVS  Matthew J. Allen Vet MB  PhD  Daniel Pascetta BS  Geoffrey T. Fosgate DVM  PhD  Diplomate ACVPM  Alan S. Litsky MD  Sc.D. 《Veterinary surgery : VS》2013,42(6):693-700

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Clinical management of Brucella suis infection in dogs and implications for public health          下载免费PDF全文

DR James  G Golovsky  JM Thornton  L Goodchild  M Havlicek  P Martin  MB Krockenberger  DJE Marriott  V Ahuja  R Malik  SM Mor 《Australian veterinary journal》2017,95(1-2):19-25

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Experimental infection with the Paderborn isolate of classical swine fever virus in 10-week-old pigs: determination of viral replication kinetics by quantitative RT-PCR,virus isolation and antigen ELISA   总被引：8，自引：0，他引：8  

Uttenthal A  Storgaard T  Oleksiewicz MB  de Stricker K 《Veterinary microbiology》2003,92(3):197-212

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