SSR markers for marker assisted selection of root-knot nematode (<Emphasis Type="Italic">Meloidogyne incognita</Emphasis>) resistant plants in cotton (<Emphasis Type="Italic">Gossypium hirsutum</Emphasis> L)     

Johnie N. Jenkins  Jack C. McCarty  Martin J. Wubben  Russell Hayes  Osman A. Gutierrez  Franklin Callahan  Dewayne Deng 《Euphytica》2012,183(1):49-54

Cotton (Gossypium hirsutum L) cultivars highly resistant to the southern root-knot nematode (RKN) [Meloidogyne incognita (Kofoid and White) Chitwood] are not available. Resistant germplasm lines are available; however, the difficulty of selecting true breeding lines has hindered applied breeding and no highly resistant cultivars are available to growers. Recently, molecular markers on chromosomes 11 and 14 have been associated with RKN resistance, thus opening the way for marker assisted selection (MAS) in applied breeding. Our study aimed to determine the utility of these markers for MAS. Cross one was RKN resistant germplasm M240 RNR × the susceptible cultivar, FM966 and is representative of the initial cross a breeder would make to develop a RKN resistant cultivar. Cross two consists of Clevewilt 6 × Mexico Wild (PI563649), which are the two lines originally used to develop the first highly RKN resistant germplasm. Mexico Wild is photoperiodic. We phenotyped the F₂ of cross one for gall index and number of RKN eggs per plant and genotyped each plant for CIR 316 (chromosome 11) and BNL 3661 (chromosome 14). From this, we verified that MAS was effective, and the QTL on chromosome 14 was primarily associated with a dominant RKN resistance gene affecting reproduction. In the first F₂ population of cross two, we used MAS to identify 11 plants homozygous for the markers on chromosomes 11 and 14, and which also flowered in long days. Progeny of these 11 plants were phenotyped for RKN gall index and egg number and confirmed as RKN highly resistant plants. Generally about 7–10 generations of RKN phenotyping and progeny testing were required to develop the original RKN highly resistant germplasms. Our results show that commercial breeders should be able to use the markers in MAS to rapidly develop RKN resistant cultivars.  相似文献   

Validation and application of a high fidelity mRNA linear amplification procedure for profiling gene expression   总被引：1，自引：0，他引：1  

Patel OV  Suchyta SP  Sipkovsky SS  Yao J  Ireland JJ  Coussens PM  Smith GW 《Veterinary immunology and immunopathology》2005,105(3-4):331-342

The need for microgram quantities of RNA for microarray experiments has hindered application of this novel technology in cell types/tissue samples with limited abundance of RNA. In this study, potential application of T7-based linear RNA amplification was investigated for use in gene expression profiling experiments where starting material is limited. Yield and integrity of amplified antisense RNA (aaRNA), microarray hybridization intensities, and fidelity of differential gene expression detected were determined for arrays generated for unamplified versus amplified RNA from the same homogenous starting pools. Total RNA was extracted from bovine spleen and fetal ovary, serially diluted to concentrations ranging from 2 microg to 500 pg and amplified. Quality and quantity of total input RNA and aaRNA were assessed by spectrophotometry, gel electrophoresis and bioanalyzer. In experiment 1, we determined the optimal amounts of aaRNA generated from 20, 40, 200 ng and 2 microg input total RNA for use in cDNA synthesis, labeling and array hybridization that would yield robust and consistent hybridization signals on a bovine oocyte cDNA microarray. In experiment 2, comparison of microarray hybridization intensities and fidelity of differential gene expression between aaRNA generated from 2, 20 and 40 ng input total RNA versus unamplified RNA (uRNA) were conducted. The hybridization intensities for each of the 7000 spots per slide for microarrays conducted using aaRNA versus uRNA were highly correlated (2 ng = 0.84, 20 ng = 0.88, 40 ng = 0.90; P < 0.01). The false positive rate was low and similar (4.0% versus 4.4%) for arrays done with uRNA and aaRNA. Ninety-seven ESTs were detected as differentially expressed in the fetal ovary versus spleen at > 1.5- or < 0.5-fold using uRNA (P < 0.05). However, the number of genes detected in arrays using aaRNA was approximately 1.5-2.5 times greater than with uRNA. Approximately, 65-70% of differentially expressed genes were common between uRNA and aaRNA arrays. Relative fold-expression (Cy3/Cy5 ratios) for 25 overlapping abundant genes was comparable for uRNA versus aaRNA arrays with 2 and 20 ng total RNA as input. Results demonstrate that T7-based linear amplification of small amounts of input RNA and use of aaRNA in microarray experiments retains fidelity of detection of differential gene expression that is relatively comparable to experiments done with uRNA and provides a potentially viable approach to facilitate gene expression profiling using limited amounts of starting material.  相似文献   

Evaluation of attract-and-kill strategy for management of cocoa pod borer,Conopomorpha cramerella,in Malaysia cocoa plantation     

Wouter Vanhove  Yan Feng  Mengmeng Yu  Ikhram Osman Hafiz  Niels Vanhoudt  Patrick L. J. Van Damme 《国际虫害防治杂志》2020,66(2):155-162

Abstract

In South-East Asia, cocoa production is dramatically affected by cocoa pod borer (CPB) infestations. As an alternative tool to chemical control, the efficacy of attract-and-kill strategy (CPB sex-pheromone as attractant and Delta trap without sticky liner sprayed with cypermethrin solution as killing station) was evaluated and compared with current standard CPB management approach as control treatment during two main cocoa harvest seasons in Malaysia (with 100 µg and 33.3 µg CPB-pheromone loading per station, respectively). In both seasons, attract-and-kill strategy was highly effective at reducing male flight activity (p?<?0.05) in attract-and-kill plots comparing with standard CPB management plots. For the percentage of CPB-infested pods, the attract-and-kill strategy (100 µg) was as good as the conventional pesticide spray applications of cypermethrin (p?=?0.083) in first season. However, it was significantly (p?=?0.021) reduced in the second season with lower pheromone loading (33.3 µg), indicating that this semiochemical based strategy is far superior to and more feasible than the currently applied conventional synthetic pesticide treatment and is therefore a good alternative in CPB integrated pest management.  相似文献   

Determining the Energy Usage Efficiency of Walnut (<Emphasis Type="Italic">Juglans Regia</Emphasis> L.) Cultivation in Turkey     

Mehmet Fırat Baran  Osman Gökdoğan  Halil İbrahim Oğuz 《Erwerbs-Obstbau》2017,59(1):77-82

The aim of this study is to reveal the energy balance of walnut in Central Anatolian Region in Turkey. This study has been conducted at the walnut cultivating facilities during the 2014–2015 production season in K?r?ehir, Konya, Nev?ehir and Ni?de provinces of Central Anatolian Region in Turkey, where walnut cultivation is intense. In the study, a total of 28 walnut cultivation facilities, yielding walnut, have been selected through Neyman method and surveys and observations have been performed in these facilities. The agricultural input energies and output energies used in walnut cultivation have been calculated to define the energy use efficiency. According to the study findings, the energy inputs in walnut cultivation are calculated respectively 17,851.33?MJ ha^?1 (74.40%) chemical fertilizer energy, 2229.87?MJ ha^?1 (9.29%) fuel energy, 1640.64?MJ ha^?1 (6.83%) irrigation water energy, 1539?MJ ha^?1 (6.41%) machine energy, 508.02?MJ ha^?1 (%2.11) chemical energy, 180.35?MJ ha^?1 (0.75%) human labour energy and 43.33?MJ ha^?1 (0.18%) farm manure energy. Production outputs have been calculated as 14,679.52?MJ ha^?1. Following the energy calculations, the output/input ratio, specific energy, energy efficiency and net energy calculations have been calculated respectively as 0.61, 30.20?MJ kg^?1, 0.03?kg MJ^?1 and ?9313.02?MJ ha^?1. Benefit-cost ratio was calculated as 1.88, by dividing the gross value of production by the total cost of production per hectare in walnut production.  相似文献   

Effects of ultraviolet A on the activity of two metabolic enzymes,DNA damage and lipid peroxidation during early developmental stages of the African catfish, <Emphasis Type="Italic">Clarias gariepinus</Emphasis> (Burchell, 1822)     

Imam A. A. Mekkawy  Usama M. Mahmoud  Alaa G. Osman  Alaa El-Din H. Sayed 《Fish physiology and biochemistry》2010,36(3):605-626

  相似文献   

Adaptation of endemic mediterranean Sternbergia candida Mathew Et T. Baytop in the continental climate of central anatolia     

Bilal Gurbuz  Neset Arslan  Khalid Mahmood Khawar  Arif Ipek  Ercument Osman Sarihan  Sebahattin Ozcan  Iskender Parmaksiz  Semra Mirici 《Scientia Horticulturae》2009,123(1):3536-103

Sternbergia candida is an important endemic bulbous plant with beautiful white flowers. It grows on the rock slopes of Baba mountains under Lebanon Cedars in the Mugla province of Turkey with Mediterranean climate. The plant is vulnerable and endangered due to illegal collection for local and foreign flower industry. Fast developing tourism industry and rapid urbanization has also shown negative implications on its population. The study reports three years successful adaptation results of S. candida in the continental climate of Ankara in the Central Anatolia. Four groups of bulbs based on diameter (>2.45, 2.15–2.44, 1.85–2.14 and <1.84 cm) were collected from its natural habitat during March, April and May 1998 and were evaluated for frequency (%) of emergence, mean number of leaves per plant, mean plant height, frequency (%) of flowering, frequency (%) of fruit setting. mean number of bulbs per plant, and mean diameter (cm) of regenerated bulbs. All categories of bulbs could be multiplied and adapted successfully with variable frequency of fruit setting. However, the highest fruit set was recorded from 2.15–2.44 cm April collected bulbs. This indicated that the bulb diameter and collection time had clear implications on multiplication, propagation and adaptation of the plant.  相似文献   

Computer aided data acquisition tool for high-throughput phenotyping of plant populations     

Raju Naik Vankadavath  Appibhai Jakir Hussain  Reddaiah Bodanapu  Eros Kharshiing  Pinjari Osman Basha  Soni Gupta  Yellamaraju Sreelakshmi  Rameshwar Sharma 《Plant methods》2009,5(1):18-10

Background  

The data generated during a course of a biological experiment/study can be sometimes be massive and its management becomes quite critical for the success of the investigation undertaken. The accumulation and analysis of such large datasets often becomes tedious for biologists and lab technicians. Most of the current phenotype data acquisition management systems do not cater to the specialized needs of large-scale data analysis. The successful application of genomic tools/strategies to introduce desired traits in plants requires extensive and precise phenotyping of plant populations or gene bank material, thus necessitating an efficient data acquisition system.  相似文献   

Effects of Freezing and Frozen Storage on Protein Functionality and Texture of Some Cephalopod Muscles     

Nalan Gokoglu  Osman Kadir Topuz  Pinar Yerlikaya  Hanife Aydan Yatmaz  Ilknur Ucak 《Journal Of Aquatic Food Product Technology》2018,27(2):211-218

The aim of this study was to investigate the effects of freezing and frozen storage on protein functionality and texture of squid (Loligo vulgaris), octopus (Octopus vulgaris), and cuttlefish (Sepia officinalis) muscles. Squid, octopus, and cuttlefish samples were cut into pieces of 4 × 4 cm. These pieces were packed in polyethylene bags. The bags were frozen in a blast freezer at ?45°C until the thermal center reached ?18°C. Frozen samples were stored in a deep freezer at ?18°C for 30 days. After freezing and during frozen storage, total soluble protein and water holding capacity decreased and total free amino acid and cooking loss increased in all cephalopod muscles. According to instrumental texture analysis results, freezing and frozen storage affected textural characteristics of squid and cuttlefish but not of octopus. Sensory hardness and chewiness values of all cephalopods increased after freezing, but elasticity values did not change. There were no significant differences between storage days in hardness values of squid and octopus. However, significant differences in hardness values of cuttlefish were observed between the 1st day of storage and the last day.  相似文献   

Fatty Acid Profiles of Different Shrimp Species: Effects of Depth of Catching     

Pinar Yerlikaya  Osman Kadir Topuz  Hanife Aydan Buyukbenli  Nalan Gokoglu 《Journal Of Aquatic Food Product Technology》2013,22(3):290-297

The aim of this study was to investigate fatty acid profiles of different shrimp species caught from deep water and shallow water. The shrimp species investigated in the study were Aristeus antennatus, Aristeomorpha foliacea, Plesionica martia, Parapenaeus longirostris, and Plesionica edwardsi from deep water; and Metapenaeus monoceros, Penaeus semisulcatus, Penaeus kerathurus, and Penaeus japonicus from shallow water.

Fatty acid composition of shrimps varied. The main fatty acids were C18:1n9, C16:0, C25:6n3, C22:5n3, and C18:0. Saturated, monounsaturated, and polyunsaturated fatty acid (PUFA) contents of P. longirostris, P. edwardsi, and M. monoceros were markedly different, respectively. The ratio of n6/n3 of the edible tissue of M. monoceros was found to be 0.795, whereas this value was 0.152 in A. foliacea. Among the species studied, the highest docosahexaenoic acid + eicosapentaenoic acid value was found for P. kerathurus. The levels of PUFAs of shallow water shrimps (ranging from 33.44 to 42.77%) were found to be higher than those of deep water shrimps (ranging from 29.68 to 33.95%). Marine animals in the upper water layers gain nutrition through phytoplankton which provides n-3 PUFA depending on solar energy. Shallow water shrimp species provide a satisfying amount of PUFA.  相似文献   

Colostral Transfer of Rinderpest Neutralizing Antibody to Offspring of Våccinated Dams          下载免费PDF全文

K. V. Singh  Omar A. Osman  Ivon F. El Cicy    Thanaa I. Baz 《Canadian journal of veterinary research》1967,31(11):295-298

The transfer of maternal antibodies to Friesian and buffalo calves born of dams vaccinated against rinderpest was through colostrum only. Colostral antibody titers at the time of parturition were higher than the serum titer. Two hours after suckling, a high level of rinderpest neutralizing antibodies was detected in the sera of newborn animals. The half-life of maternal antibodies in buffalo and Friesian calves was found to be approximately 33 and 29 days respectively. By the age of 7-8 months, 60 per cent of buffalo calves and 80 per cent of Friesian calves had no detectable levels of rinderpest neutralizing antibody.  相似文献