Establishment and characterization of four canine melanoma cell lines     

Inoue K  Ohashi E  Kadosawa T  Hong SH  Matsunaga S  Mochizuki M  Nishimura R  Sasaki N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(11):1437-1440

Four canine melanoma cell lines were established from the subcutaneous, oral gingival and mucosal melanoma tissues at the primary and metastatic sites. These cell lines were designated as CMeC-1, CMeC-2, KMeC and LMeC. The cells were spindles in shape, similar to that of primary tumor cells. The doubling times of these cells ranged from 34.1 +/- 5.61 to 57.9 +/- 3.28 hr and their chromosome number ranged from 46 to 80. When transplanted into nude mice, CMeC-1 and LMeC produced tumors, whereas CMeC-2 and KMeC did not. The morphology of the tissue formed by xenotransplantation of these cells was similar to their primary tumors.  相似文献   

X-ray fluorescence spectrometry of asteroid Itokawa by Hayabusa     

Okada T  Shirai K  Yamamoto Y  Arai T  Ogawa K  Hosono K  Kato M 《Science (New York, N.Y.)》2006,312(5778):1338-1341

X-ray fluorescence spectrometry of asteroid 25143 Itokawa was performed by the x-ray spectrometer onboard Hayabusa during the first touchdown on 19 November 2005. We selected those data observed during relatively enhanced solar activity and determined average elemental mass ratios of Mg/Si = 0.78 +/- 0.09 and Al/Si = 0.07 +/- 0.03. Our preliminary results suggest that Itokawa has a composition consistent with that of ordinary chondrites, but primitive achondrites cannot be ruled out. Among ordinary chondrites, LL- or L-chondrites appear to be more likely than H-chondrites. No substantial regional difference was found on the asteroid surface, indicating its homogeneity in composition.  相似文献   

A case of feline pancreatitis     

Sakai M  Harada K  Matsumura H  Takahashi T  Koie H  Yamaya Y  Watari T  Shibuya H  Sato T  Tokuriki M  Hasegawa A 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(12):1331-1333

A 14-year-old, spayed female, domestic shorthair cat was referred to us with anorexia, pyrexia, and jaundice. Total bilirubin (TBIL) and feline trypsin-like immunoreactivity (fTLI) levels were remarkably high. Based on laparoscopic biopsy of the pancreas, the cat was diagnosed as having pancreatitis. As a result of treatment with a synthetic protease inhibitor and corticosteroid, the TBIL and fTLI values returned to normal and the clinical course was good.  相似文献   

In vitro and in vivo developmental ability of oocytes derived from porcine primordial follicles xenografted into nude mice     

Kikuchi K  Kaneko H  Nakai M  Noguchi J  Ozawa M  Ohnuma K  Kashiwazaki N 《The Journal of reproduction and development》2006,52(1):51-57

We evaluated the developmental ability of oocytes in porcine primordial follicles xenografted into nude mice. Ovarian tissues from 20-day-old piglets, in which most of the follicles were primordial, were transplanted under the kidney capsules of ovariectomized nude mice. Forty-nine to 89 days after grafting (mean +/- SEM, 66.9 +/- 1.9 days; n = 64), the host mice showed the presence of cornified epithelial cells in their vaginal smears for the first time. The mice were then treated with 4 IU of equine chorionic gonadotropin (eCG) 60 days after first detection of vaginal cornification. Oocytes were collected from the host mice 48 h after treatment with eCG, and then matured. The maturation rates, based on the incidence of first polar body, ranged from 25.1% to 42.5%. They were then fertilized in vitro and cultured in vitro for 6 days, or transferred into estrous-synchronized recipients and recovered after 6 days. On Day 6 of culture, 15.4% of the matured oocytes had cleaved to the 2- to 8-cell stage. However, neither the embryos cultured in vitro nor those transferred and recovered developed to advanced embryonic stages, such as morulae or blastocysts. This result suggests that the developmental ability of xenografted oocytes is insufficient, even after in vitro maturation. Further strategies, such as improvement of hormonal treatment for host mice, are required to enable oocytes in xenografted ovarian tissues to acquire the cytoplasmic maturation necessary for embryonic development.  相似文献   

Hemophilia B in a crossbred Maltese dog     

Nakata M  Sakai M  Sakai T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(11):1223-1224

A crossbred Maltese dog, 6-year-old, male, was presented to us for examination due to coagulopathy. On examination of blood coagulation screening tests, activated partial thromboplastin time (APTT) was markedly prolonged (63.6 sec). Therefore, a defect in the intrinsic pathway of coagulation was suspected. An additional serum test was also examined and APTT was returned to within the normal range. Furthermore, factor IX coagulation activity was markedly low (2.3%). On the basis of these results, the dog was diagnosed with hemophilia B. The dog has since been presented to us because of hemorrhage problems again after 5, 10, and 16 months, but blood transfusions have maintained good control of its coagulopathy for more than two years.  相似文献   

Phenotypic analysis of hepatic T lymphocytes in a dog with chronic hepatitis     

Sakai M  Otani I  Ishigaki K  Edamura K  Takahashi T  Koie H  Yamaya Y  Watari T  Shibuya H  Sato T  Kanayama K  Tokuriki M  Hasegawa A 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(11):1219-1221

We evaluated hepatic T lymphocyte phenotypes in a dog with chronic hepatitis. Before treatment, numerous CD3+ lymphocytes were demonstrated in the liver, and the ratio of CD4+/CD8+ was remarkably high (2.96; reference range, 0.33+/-0.12). After treatment, CD3+ lymphocyte infiltration in the liver was reduced, and the ratio of CD4+/CD8+ decreased to 0.31. Therefore, hepatic T lymphocytes, especially CD4+ lymphocytes, might play a central role in the pathogenesis of this dog with chronic hepatitis.  相似文献   

Biogenic potassium salt particles as seeds for secondary organic aerosol in the Amazon     

C Pöhlker  KT Wiedemann  B Sinha  M Shiraiwa  SS Gunthe  M Smith  H Su  P Artaxo  Q Chen  Y Cheng  W Elbert  MK Gilles  AL Kilcoyne  RC Moffet  M Weigand  ST Martin  U Pöschl  MO Andreae 《Science (New York, N.Y.)》2012,337(6098):1075-1078

The fine particles serving as cloud condensation nuclei in pristine Amazonian rainforest air consist mostly of secondary organic aerosol. Their origin is enigmatic, however, because new particle formation in the atmosphere is not observed. Here, we show that the growth of organic aerosol particles can be initiated by potassium-salt-rich particles emitted by biota in the rainforest. These particles act as seeds for the condensation of low- or semi-volatile organic compounds from the atmospheric gas phase or multiphase oxidation of isoprene and terpenes. Our findings suggest that the primary emission of biogenic salt particles directly influences the number concentration of cloud condensation nuclei and affects the microphysics of cloud formation and precipitation over the rainforest.  相似文献   

Antibody response in vaccinated pregnant mares to recent G3BP[12] and G14P[12] equine rotaviruses     

Manabu Nemoto  Hiroshi Tsunemitsu  Harutaka Murase  Yasuo Nambo  Shinsuke Sato  Yasuhiro Orita  Hiroshi Imagawa  Hiroshi Bannai  Koji Tsujimura  Takashi Yamanaka  Tomio Matsumura  Takashi Kondo 《Acta veterinaria Scandinavica》2012,54(1):63

Background

Both the G3P[12] and the G14P[12] type of equine group A rotavirus (RVA) have recently become predominant in many countries, including Japan. G3 types are classified further into G3A and G3B. The G3A viruses have been circulating in Europe, Australia, and Argentina, and the G3B viruses have been circulating in Japan. However, only an inactivated vaccine containing a single G3BP[12] strain is commercially available in Japan. To assess the efficacy of the current vaccine against recently circulating equine RVA strains, we examined antibody responses in pregnant mares to recent G3BP[12] and G14P[12] strains by virus neutralization test.

Findings

After vaccination in five pregnant mares, the geometric mean serum titers of virus-neutralizing antibody to recent G3BP[12] strains increased 5.3- to 7.0-fold and were similar to that against homologous vaccine strain. Moreover, antibody titers to recent G14P[12] strains were also increased 3.0- to 3.5-fold.

Conclusions

These results suggest that inoculation of mares with the current vaccine should provide foals with virus-neutralizing antibodies against not only the G3BP[12] but also the G14P[12] RVA strain via the colostrum.  相似文献   

Roles of genes regulated by two paternally methylated imprinted regions on chromosomes 7 and 12 in mouse ontogeny     

Kawahara M  Kono T 《The Journal of reproduction and development》2012,58(2):175-179

We studied the longevity of mice produced without sperm using the genomes of oocytes that are already committed to a germline cell lineage. The first sperm-free mouse "KAGUYA", which we term 'bi-maternal mouse', was born on 3 February, 2003. Bi-maternal embryos were generated using 2 sets of female genomes--one derived from fully grown oocytes from normal adults and the other from non-growing oocytes from newborn pups. These genomes were combined by nuclear transfer. We refined the technique for generating bi-maternal mice and found that genetic manipulations in only 2 regions--the imprinting centres of Igf2-H19 and Dlk1-Gtl2--on chromosomes 7 and 12 of the newborn pups allowed us to generate bi-maternal mice at a high rate. Studying bi-maternal conceptuses and mice provides further insight into the mechanisms by which paternally methylated imprinted genes regulate mammalian ontogenesis.  相似文献   

Mitigation of pyrexia by a Th-1-biased IgG subclass response after infection with equine herpesvirus type 1 in horses pre-immunized with inactivated vaccine     

Bannai H  Tsujimura K  Kondo T  Nemoto M  Yamanaka T  Sugiura T  Kato T  Maeda K  Matsumura T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(6):791-795

The immunoglobulin G (IgG) subclass response was investigated in horses with or without pyrexia after natural infection with equine herpesvirus type 1 (EHV-1) in the field. All horses were kept at the training centers of the Japan Racing Association and were immunized with an inactivated EHV-1 vaccine before EHV-1 infection. An IgG subclass response dominated by IgGa and IgGb was induced in horses without pyrexia after EHV-1 infection. In contrast, horses that developed pyrexia showed increased IgGc and IgG (T) subclass production in addition to IgGa and IgGb. Although inactivated EHV-1 vaccines are considered to induce a mainly Th-2-biased response, these results indicated that the responses in horses inoculated with inactivated EHV-1 vaccine were not uniform, and that horses with a Th-1-biased response were likely to be protected from pyrexia.  相似文献