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11.
Severe respiratory disease, associated with seroconversion to bovine respiratory syncitial virus (BRSV), caused the death of two cattle and necessitated antibiotic treatment of 70 calves (rectal temperature of 39.6°C or greater) from a group of 96 (73%) during an 8-day period. Tilmicosin injection resulted in a reduction in median rectal temperature from 40.3°C to 39.2°C and 39.0°C for the first and second days after treatment. The rectal temperature was 39.5°C or lower in 72% (48 of 67) and 96% (64 of 67) of cattle 1 and 2 days after tilmicosin treatment, respectively. Ten cattle were re-treated with tilmicosin 6 to 16 days after the first treatment. Our study demonstrated that bovine respiratory syncytial virus infection could cause severe respiratory disease in a beef herd that had no previous history of BRSV-related disease. Secondary bacterial invasion after BRSV infection was controlled effectively by tilmicosin treatment but repeat antibiotic treatments were occasionally necessary due to bacterial re-infection of the respiratory tract.  相似文献   
12.
This review summarises current control measures for clinical paratuberculosis (Johne’s disease; JD) in New Zealand pastoral livestock. Most New Zealand sheep, deer, beef and dairy cattle herds and flocks are infected by Mycobacterium avium ssp. paratuberculosis (Map). Dairy cattle and deer are mostly infected with bovine (Type II), and sheep and beef cattle with ovine (Type I) strains. Control in all industries is voluntary. While control in sheep and beef cattle is ad hoc, the dairy and deer industries have developed resources to assist development of farm-specific programmes.

The primary target for all livestock is reduction of the incidence rate of clinical disease rather than bacterial eradication per se. For dairy farms, a nationally instituted JD-specific programme provides guidelines for risk management, monitoring and testing clinically suspect animals. While there is no formal programme for sheep farms, for those with annual prevalences of clinical disease >2%, especially fine wool breeds, vaccination may be a cost effective control option. The deer industry proactively monitors infection by a national abattoir surveillance programme and farmers with an apparent high disease incidence are encouraged to engage with a national network of trained consultants for management and control advice. Evaluation of the biological and economic effectiveness of control in all industries remains to be undertaken. Nevertheless, opportunities exist for farmers, who perceive significant JD problems in their herds/flocks, to participate in systematic best-practice activities that are likely to reduce the number of clinical infections with Map on their farms, and therefore the overall prevalence of JD in New Zealand’s farming industries.  相似文献   

13.
An extensive field comparison of the gamma interferon (IFN-gamma) assay and the single intradermal tuberculin test for the diagnosis of bovine tuberculosis was conducted in Australia. The specificity of the IFN-gamma assay was determined by testing more than 6000 cattle from tuberculosis-free herds and varied from 96.2% to 98.1%, depending on the cut-off point chosen to define a positive reactor. For the sensitivity trial, cattle from herds being de-populated because of bovine tuberculosis were examined with both assays. The sensitivity of the IFN-gamma assay was shown to be significantly higher than the single intradermal tuberculin test and varied from 76.8% to 93.6% depending on the method of interpretation. A maximum overall sensitivity of 95.2% was obtained by testing with the IFN-gamma and the tuberculin test in parallel. The superior sensitivity of the IFN-gamma assay and the ability to adjust the sensitivity of the system depending on the task involved, will provide the Australian Tuberculosis Eradication Campaign with a valuable additional test to enable it to accomplish its goals.  相似文献   
14.
SUMMARY A multivalent footrot vaccine has been developed, containing pilus antigens produced in recombinant Pseudomonas aeruginosa and representing all nine serogroups of Dichelobacter (Bacteroides) nodosus commonly recognised in the field. The responses of sheep to the multivalent vaccine have been compared with those to monovalent vaccines representing only a single serogroup. Antigenie competition between serogroups occurred in sheep immunised with the multivalent formulation, but high levels of protection were still achieved. The study showed that in multivalent footrot vaccines, antigenic competition is predominantly due to the presence of a family of immunologically-related pilus antigens rather than to interference by extraneous proteins.  相似文献   
15.
Global Oscillation Network Group data reveal that the internal structure of the sun can be well represented by a calibrated standard model. However, immediately beneath the convection zone and at the edge of the energy-generating core, the sound-speed variation is somewhat smoother in the sun than it is in the model. This could be a consequence of chemical inhomogeneity that is too severe in the model, perhaps owing to inaccurate modeling of gravitational settling or to neglected macroscopic motion that may be present in the sun. Accurate knowledge of the sun's structure enables inferences to be made about the physics that controls the sun; for example, through the opacity, the equation of state, or wave motion. Those inferences can then be used elsewhere in astrophysics.  相似文献   
16.
AIM: To obtain an estimate of the incidence of assistance at calving in primiparous (first-calving) beef heifers and the prevalence of breeding heifers at 15 months of age in New Zealand in 2006,and to identify factors contributing to farmers' decisions regarding breeding strategies for heifers, using a survey of beef cattle farmers.

METHODS: A questionnaire was sent to farmers listed in a Massey University database and to members of selected breed societies, as well as published in an industry newspaper; 331 valid responses were received. Information was gathered on the age and number of primiparous heifers, number of heifers assisted, and the importance of various reasons for and against breeding heifers at 15 months of age. Respondents were also required to outline the criteria used for selecting bulls to join with heifers, and the strategies used to manage dystocia in primiparous heifers.

RESULTS: Sixty-five (95% CI=58–71)% of respondents had only 2-year-old primiparous heifers in 2006, whilst a further 11 (95% CI=8–16)% had both 2- and 3-year-old primiparous heifers. The mean reported incidence of assisted calving was 7.0 (95% CI=6.4–7.5)% for 2-year-old primiparous heifers and 1.7 (95% CI=1.2–2.2)% for 3-year-old primiparous heifers. The reported incidence of assistance at calving within individual herdsranged from 0 to 100% for 2-year-old heifers. Respondents with bull-breeding herds most commonly observed their primiparous 2-year-old heifers twice daily, whilst respondents with commercial herds most commonly observed them once daily during calving. The most important reason for breeding heifers at 15 months of age was “increased profit”, whereas the most important reason for not breeding them at that age was “concern about rebreeding performance of 2-year-old heifers”. Estimated breeding value (EBV) for birth weight was the factor considered most frequently when selecting bulls to join with maiden heifers; age of bull and body shape of bull were the next most frequently considered factors. Selection of an appropriate bull was the most common strategy used to manage dystocia in 2-year-old beef heifers.

CONCLUSIONS: The majority of respondents calved their heifers at 2 years of age, and “increased profit” was the primary motivator. Concern about the rebreeding performance of 2-year-old heifers was the most important reason among the remainder of respondents for not breeding heifers at 15 months of age. Dystocia in 2-year-old heifers was “not a problem” or “a minorproblem” in most herds, but there was much variation amongst herds.  相似文献   
17.
AIM: To investigate the role of free-living animals such as spar- rows, rodents and flies as potential reservoirs of Campylobacter spp on a dairy farm, and to assess the genetic diversity among Campylobacter isolates from the farm and an urban source.

METHODS: A total of 290 samples (bovine, passerine and ro- dent faeces, and whole flies) were collected from a large com- mercial dairy farm in the Manawatu district in New Zealand, and from faeces from urban sparrows in a nearby city. Other samples collected from the dairy farm included five from silage, two from aprons worn by workers during milking, two from workers' boots and two from water in troughs in a paddock. Isolates of thermophilic Campylobacter spp were identified mor- phologically and phenotypically and further characterised mo- lecularly using pulsed-field gel electrophoresis (PFGE) and the restriction enzyme SmaI.

RESULTS: Campylobacter jejuni was the only Campylobacter species isolated from all samples. The highest prevalence was found in faeces from dairy cows (54%), followed by faeces from sparrows from the urban area (40%) and the farm (38%), and from rodents (11%) and whole flies (9%). Other samples from the farm environment such as silage, trough water, and work- ers' aprons and boots were also positive for C. jejuni. Of the 22 restriction patterns obtained, seven were common to more than one source.

CONCLUSIONS: Cattle, sparrows, rodents and flies are po- tential reservoirs of C. jejuni on dairy farms. Identical clones of C. jejuni carried by cattle, sparrows, flies and rodents prob- ably indicate a common source of infection. The high level of asymptomatic carriage of C. jejuni by healthy dairy cows could be sufficient to maintain infections within the dairy farm sur- roundings via environmental contamination.  相似文献   
18.
AIM: To determine the effects of liveweight (LW) and use of teaser rams prior to mating on both the mating performance and pregnancy rate of ewe hoggets.

METHODS: Romney hoggets (n=841) were weighed and randomly assigned to one of two treatment groups; either exposed to seven harnessed, vasectomised (teaser) rams for 17 days prior to the breeding period (n=283); or not exposed to teaser rams (unteased, n=558). Harnessed Perendale rams were then introduced for two oestrous cycles. Using crayon marks, hoggets were identified as having been mated during the first 17 days only, during the second 17 days only, during both periods, or not mated. All mated hoggets were then scanned for pregnancy using ultrasound, 48 days after the end of the breeding period, and identified as being either non-pregnant, or as single-, twinor triplet-bearing.

RESULTS: Hoggets mated in either the first 17 days only or in both the first and second 17-day periods were heavier than those mated in the second period only (p<0.001). Those mated in the first or second 17-day period only, and those mated in both periods were all heavier prior to mating than those not mated at all (p<0.001). Twin-bearing hoggets were heavier than both their single-bearing and non-pregnant counterparts (p<0.001). A greater proportion of the teased hoggets were mated in the first 17 days only compared with unteased hoggets (62.6 vs 32.1%; p<0.001). In contrast, more of the unteased hoggets were marked in the second 17-day period only (p<0.001). A greater proportion of mated, unteased hoggets were found to be non-pregnant in comparison to their teased counterparts (17.8 vs 11.2%; p=0.02).

CONCLUSIONS: Heavier hoggets at breeding were more likely to be mated in the first 17 days of the breeding period and more likely to be twin-bearing. Use of teaser rams for 17 days prior to the breeding period increased the proportion of hoggets mated in the first 17 days, increased conception rates, and thus increased the proportion of pregnant hoggets. These results indicate that farmers should utilise teasers for 17 days prior to commencement of the breeding period and avoid mating hoggets that weigh below 36 kg.  相似文献   
19.
20.
Abstract

AIM: To determine the effect of age and prior use of mature rams at a given ram-to-ewe ratio, and the effect of body condition on breeding performance and pregnancy rate of ewe hoggets.

METHODS: Ewe hoggets (n=733) aged 7–8 months were weighed and their body condition scored, then randomly assigned to one of three treatment groups (Day 0) and joined with either four two-tooth rams (20 months of age) not used previously (n=244; Two-tooth), four mixed-aged mature rams that had not been used earlier in the season (n=244; Mature-fresh), or four mixed-aged mature rams that had been used with mature ewes immediately prior to joining with hoggets (n=245; Mature-used). The breeding period was 34 days. Ewe hoggets were identified as having been marked during the first 17 days only, during both 17-day periods, during the second 17 days only, or not marked. Hoggets were re-weighed on Day 34, and pregnancy status determined using ultrasound on Day 92. The breeding soundness of the rams was assessed on Days ?34 and ?1.

RESULTS: Semen samples obtained from the rams did not differ significantly in any of the parameters measured (p>0.05). Ewe hoggets joined with Mature-fresh rams were less likely (p<0.05) to be marked in the second 17 days of breeding only than those joined with either Two-tooth or Mature-used rams. No other breeding parameters were affected by breeding group (p>0.05). Hoggets marked in the first 17 days only were heavier (p<0.05) at Day 0 than those marked in the second 17 days only or not marked. Hoggets diagnosed as twin-bearing were heavier (p<0.05) than non-pregnant or single-bearing hoggets. Those hoggets marked in the first 17 days only had a greater (p<0.05) body condition score (BCS) than those marked in the second 17 days only or not marked at all. These differences were no longer apparent after correction for liveweight (LW).Correction for LW at Day 0 or change in LW during the breeding period did not affect the results for breeding performance.

CONCLUSIONS: Under the conditions of this study, two-tooth rams and mature rams that had been used previously were just as suitable as mature rams that had not been used previously for breeding with ewe hoggets. Further studies are warranted to verify this result. The re-use of rams without reducing breeding performance would reduce breeding costs and may make breeding hoggets a more viable option for farmers. The BCS of ewe hoggets affected breeding performance, and can thus be used to identify those animals most suitable for breeding.  相似文献   
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