Oxygen limitation and tissue metabolic potential of the African fish Barbus neumayeri : roles of native habitat and acclimatization     

Mery L Martínez  Erin L Raynard  Bernard B Rees  Lauren J Chapman 《BMC ecology》2011,11(1):2

Background  

Oxygen availability in aquatic habitats is a major environmental factor influencing the ecology, behaviour, and physiology of fishes. This study evaluates the contribution of source population and hypoxic acclimatization of the African fish, Barbus neumayeri, in determining growth and tissue metabolic enzyme activities. Individuals were collected from two sites differing dramatically in concentration of dissolved oxygen (DO), Rwembaita Swamp (annual average DO 1.35 mgO₂ L^-1) and Inlet Stream West (annual average DO 5.58 mgO₂ L^-1) in Kibale National Park, Uganda, and reciprocally transplanted using a cage experiment in the field, allowing us to maintain individuals under natural conditions of oxygen, food availability, and flow. Fish were maintained under these conditions for four weeks and sampled for growth rate and the activities of phosphofructokinase (PFK), lactate dehydrogenase (LDH), citrate synthase (CS), and cytochrome c oxidase (CCO) in four tissues, liver, heart, brain, and skeletal muscle.  相似文献   

Low-Salinity Acclimation of Juvenile Marine Goliath Grouper Epinephelus itajara     

Frank A. Chapman  Lury N. García  Victor J. Atencio  Robin J. Muñoz  Alfonso Silva  Héctor Flores 《Journal Of Applied Aquaculture》2013,25(2):179-186

The ability of marine goliath grouper to survive in waters of low salinity was investigated by collecting juvenile individuals from the wild in full-strength seawater and either transferring them directly to fresh water or acclimating them over a 96-h period to fresh water. Juvenile goliath grouper (n = 49; mean 23.4 cm SL; SD 4.1; mean 339.1 g; SD 89.3) tolerated transfers from seawater (30 ppt) to freshwater (<1 ppt), and survived thereafter for a period of 28 d in strictly fresh water, with no observed adverse effects. However, the salinity tolerance was influenced by rate of exposure, as no deaths were observed when the fish were given a gradual decrease in salinity, but 60% of the individuals died when transferred abruptly from seawater into fresh water.  相似文献   

Glucocorticoid‐deficient hypoadrenocorticism secondary to intravascular lymphoma in the adrenal glands of a dog          下载免费PDF全文

ME Buckley  PS Chapman  A Walsh 《Australian veterinary journal》2017,95(3):64-67

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Impacts of resource extraction on forest structure and diversity in Bardia National Park,Nepal   总被引：1，自引：0，他引：1  

Shova Thapa  Daniel S. Chapman 《Forest Ecology and Management》2010

Repeated biomass harvesting for human livelihood needs can cause significant changes in forest structure, composition and diversity. Such impacts have often been associated with the distance to villages and their size, but the effects of individual villages in relation to their characteristics have been little studied. Focussing on the issues around communities’ impacts on the forest, this research aimed to understand how resource extraction by two Buffer Zone villages in Nepal's Bardia National Park affects forest structure and diversity. Tree density, basal area, diameter at breast height (DBH), species richness, two diversity indices and disturbance indices were recorded in 108 plots in 12 transects, from March till May 2005, within the park along the village boundaries, where resource extraction occurs, and in the core park area which is isolated from human disturbance. Forest subject to resource extraction had a lower density of trees, smaller DBH and lower species richness and diversity. Forest disturbance declined with distance from the villages but the two villages differed widely in their impact on the forest. Resource use was more intensive in Shivapur village and percentage of trees lopped, average lopping intensity and numbers of dung piles were statistically significant. This was mainly due to the lack of access to Buffer Zone community Forest for Shivapur households. Interviews with villagers suggested that there have been changes in species availability compared to 5 years ago. Households also underestimated the impact of their resource extraction, which may prevent them from changing their behaviour to benefit conservation. It is clear that understanding rural communities’ needs, their framing of resource extraction and utilisation, and dependence on forest resources will be very helpful for long term conservation measures.  相似文献   

Bioavailability as a Factor in Risk Assessment of Metal-Contaminated Soil   总被引：1，自引：0，他引：1  

E. Emily V. Chapman  G?ran Dave  John D. Murimboh 《Water, air, and soil pollution》2012,223(6):2907-2922

To accurately assess the risks of metal contaminants in soil, the bioavailability of the metals need to be considered. The bioavailable concentrations determined from homogenized, dried soils are not necessarily representative of in situ conditions of undisturbed field soils. In this study, we investigated the accumulation of metals in wheat (Triticum aestivum) grown in undisturbed contaminated (Pb, Zn, Cu, and Cd) soil cores, in relation to total soil, leachate, and diffusive gradients in thin films (DGT)-labile metal concentrations. Despite the fact that many of the samples contained metal concentrations above guideline values, no significant effects were observed on wheat growth. The bioavailability of metals in the most contaminated samples was estimated to be medium to low, possibly explaining why few effects were observed in the bioassay. For Cu, Zn, and especially for Pb, leachate and DGT-labile concentrations were better predictors of uptake by wheat than total concentrations based on correlations. It is suggested that DGT and leachate concentrations in combination with bioassays in undisturbed soil cores can be used to account for metal bioavailability in soil. These tests could be used during the ecological risk screening stage, in conjunction with total concentrations and guideline values to better estimate receptor exposure.  相似文献   

Differences in nitrogen‐assimilating enzyme activity in halophyte species are habitat‐related          下载免费PDF全文

Grace M. Cott  Deborah V. Chapman  Marcel A. K. Jansen 《植物养料与土壤学杂志》2014,177(5):705-713

The chemical form and content of available nitrogen (N) in salt marsh substrates varies considerably. On the western coast of Ireland, habitats designated as Ombrogenic Atlantic salt marshes were formed on ombrogenic peat substrate. The peat substrate in these systems has three times more ammonium than substrate from adjacent salt marsh habitats on sand and mud substrate. This study examined the extent to which the high concentration of ammonium in peat salt marsh substrate influences the N‐ assimilating enzyme activity of halophytes and the extent to which N metabolism differs between species. Specifically, this work investigated whether plants from peat salt marshes are more likely to assimilate ammonium than plants from non‐peat substrates. Four halophyte plant species—Armeria maritima, Aster tripolium, Plantago maritime, and Triglochin maritime—were sampled from various saltmarsh habitats including three sites on peat substrate and three on non‐peat substrate, comprising sand, mud and sand/mud. The activities of N‐metabolising enzymes—glutamine synthetase (GS), glutamate synthase, glutamate dehydrogenase (GDH), and nitrate reductase (NR)—were quantified in shoot and root parts. Root GS activity in Armeria maritima and shoot GS activity in Triglochin maritima were positively correlated with increasing soil ammonium levels. Root NR activity in Aster tripolium and shoot NR activity in Plantago maritima were significantly higher in plants grown on non‐peat substrates than peat substrates. The shoot : root GS activity ratio in Triglochin maritima on peat substrate was more than double the ratio on non‐peat substrates. It is concluded that all species tested displayed differences in N‐metabolising activities depending on the chemical form and/or concentration of N in the substrate, while three out of the four species were capable of taking advantage of the high levels of ammonium in peat substrates.  相似文献   

Uptake of metronidazole in Artemia at different developmental life stages     

Rodriguez L  Livengood EJ  Miles RD  Chapman FA 《Journal of aquatic animal health》2011,23(2):100-102

The purpose of this study was to examine the capacity of live brine shrimp Artemia spp. to accumulate metronidazole at different developmental life stages. Metronidazole is used in fish as an antiparasitic medication. An effective drug delivery method is to enrich the Artemia with metronidazole and offer them as live feed to the infected fish, usually ornamental species and other small fishes. Artemia cysts were hatched and then soaked in a metronidazole solution (0.05%) at instars 1-3 of larval development. Our findings indicated that Artemia were able to accumulate metronidazole at levels considered therapeutic to other animals and humans (25-100 mg/kg). However, the levels varied depending on the stage of larval development. Artemia accumulated the highest levels of metronidazole (137-143 mg/kg) when they started filter feeding (instar 2), whereas newly hatched Artemia (instar 1) contained the lowest level (85 mg/kg). Based on this study and a review of the literature, a new protocol recommended for enriching Artemia with metronidazole consists of soaking the Artemia in a 0.05% metronidazole solution for 3 h at room temperature. Because metronidazole is relatively insoluble in water, it must first be dissolved in warm water with continuous stirring.  相似文献   

Early-successional vegetation changes after roadside prairie restoration modify processes related with soil functioning by changing microbial functional diversity     

Pablo García-Palacios  Matthew A. Bowker  Stephen J. Chapman  Santiago Soliveres  Antonio Gallardo  César Guerrero 《Soil biology & biochemistry》2011,43(6):1245-1253

Because of their rapidly changing vegetation dynamics and harsh environmental conditions, roadside prairies in semi-arid regions represent an exceptional study system in which to investigate the effects of plant-soil interactions on ecosystem functioning. We conducted a two-year field experiment on two roadside embankments in semi-arid central Spain differing in construction age to answer the following questions: (i) do commonly used restoration treatments (hydroseeding, fertilization and irrigation) affect soil microbial functional diversity and processes related to soil functioning (basal respiration, total N and P and in situ N availability rate)? (ii) what portion of plant effects on processes related to soil functioning is mediated indirectly by microbial functional diversity? Except for a small and negative irrigation effect on the microbial functional diversity in the three-year old site, the restoration treatments employed did not affect this variable. Fertilization increased plant diversity, an effect likely mediated by the enhanced soil nutrient availability with this treatment at early stages of secondary succession. In contrast, hydroseeding did not affect processes related to soil functioning. The total effect of the plant community on these processes was higher than that of the microbial functional diversity alone, suggesting that the studied slopes are to the greater extent regulated by plants. However, soil microbes are a key proximate influence in the system, as the indirect effects of plant community on soil functioning processes mediated by soil microbes represented 37-41% of the total plant effects observed. Our results indicate that the restoration of recently built slopes can potentially be improved with treatments that promote plant compositional shifts, such as fertilization, or alter soil function, such as the enhancement of soil microbial functional diversity. They also highlight that plant-soil interactions are an important process that can be manipulated for restoration purposes in early-successional stages, especially in nutrient-poor semi-arid ecosystems.  相似文献   

Parent's responses to nutrient claims and sports celebrity endorsements on energy-dense and nutrient-poor foods: an experimental study     

Dixon H  Scully M  Wakefield M  Kelly B  Chapman K  Donovan R 《Public health nutrition》2011,14(6):1071-1079

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Rooting of potato cuttings     

H. W. Chapman  M. D. Clegg 《American Journal of Potato Research》1961,38(5):158-162

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