Profiling nutraceuticals in bael [Aegle marmelos (L.) Correa] at various stages of fruit development     

A. K. Bhattacherjee  A. Dikshit  P. Chethan Kumar  D. Pandey  D. K. Tandon 《The Journal of Horticultural Science and Biotechnology》2016,91(2):169-174

Bael [Aegle marmelos (L.) Correa], a medicinally important fruit tree grown on the Indian sub-continent, contains two bio-active furanocoumarins, marmelosin and psoralen, with pharmacological properties, plus two powerful groups of natural anti-oxidants, polyphenols and tannins. The present investigation aimed to measure the levels of these nutraceuticals at various stages of fruit development [150–345 days after fruit set (DAFS)] in two promising selections of bael from the Institute, CISH B-1 and CISH B-2, using a simple high performance liquid chromatography (HPLC) technique. Marmelosin and psoralen were extracted from 5 g of homogenised fruit mesocarp using benzene, while tannins were extracted as tannic acid with 80% (v/v) methanol in water. Polyphenol concentrations were measured spectrophotometrically at 760 nm using Folin-Ciocalteu reagent. The concentrations of marmelosin declined from 1,660 and 726 µg g^?1 at 150 DAFS, to 523 and 170 µg g^?1 at 345 DAFS in CISH B-1 and CISH B-2, respectively, whereas psoralen concentrations decreased from 216 and 41 µg g^?1 to 102 and 9 µg g^?1 during the same growth period in CISH B-1 and CISH B-2, respectively. Tannic acid concentrations decreased from 1.65 and 2.02 g 100 g^?1 at 150 DAFS, to 1.52 and 1.54 g 100 g^?1 at 345 DAFS in CISH B-1 and CISH B-2, respectively. The concentrations of total polyphenols did not show any significant changes in either bael selection over the same growth period. The concentrations of marmelosin and psoralen in CISH B-1 were highest in October (150 DAFS), while these values peaked in November (190 DAFS) in CISH B-2. The results suggest that fruit harvested at an early stage of growth (October–November) contained higher concentrations of nutraceuticals, increasing their suitability for fruit processing and use in the phyto-pharmaceutical industries.  相似文献   

Prevention and therapy of inflammation of the swimbladder in the carp (Cyprinus carpio L.)     

P K Pandey 《Archiv fuer experimentelle veterinaermedizin》1974,28(4):595-609

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The effect of a perennial cover crop on net soil N mineralization and microbial biomass carbon in coconut plantations in the humid tropics     

C. B. Pandey  M. Begum 《Soil Use and Management》2010,26(2):158-166

The decrease in soil fertility in agroecosystems due to continuous harvesting, loss of fine soil and oxidation of soil organic carbon (SOC) is well known. This study evaluates for a humid tropical climate in South Andaman Island, India, the impact of a 15-yr old Pueraria cover crop (CC) under a coconut plantation, with and without phosphorus (P) application, on the soil N mineralization rate (NMR), the mineral N pool (NH₄⁺-N and NO₃⁻-N), microbial biomass carbon (MB-C) and SM under four treatments, (1) no cover crop (NCC), (2) no cover crop + phosphorus (NCC+P), (3) CC, and (4) cover crop + phosphorus (CC+P) during three seasons, wet (May–October), post-wet (November–January) and dry (February–April). The NCC treatment served as a control. In addition, an ex-situ experiment was conducted to verify the effect of P application on NMR and MB-C under 100% field capacity (FC), 50 and 25% FC representing the different seasons. The NMR, mineral N pool and MB-C increased by 37, 46 and 41%, respectively under the CC compared to the control. SOC and fine soil particles were also greater under the CC by 41 and 461%, respectively, compared to the control. The application of P to the CC increased soil N mineralization, the mineral N pool and MB-C by 33, 16 and 14%, respectively. The amount of mineralized N was greater under the CC and CC+P treatments by 39 and 73%, respectively than the control. The ex-situ experiment showed that the P application increased NMR and MB-C, but the increases were highest in the 50% FC and lowest in 100% FC. It is proposed for the humid tropics that a CC could be used for enhancing SOC and increasing soil N mineralization under coconut plantations and other similar agroecosystems.  相似文献   

Antifungal activity of the stem bark of Ailanthus excelsa     

Joshi BC  Pandey A  Chaurasia L  Pal M  Sharma RP  Khare A 《Fitoterapia》2003,74(7-8):689-691

The methanol extract of stem barks of Alianthus excelsa was partitioned with chloroform. The chloroform extract showed fungistatic and fungicidal activity against Aspergillus niger, A. fumigatus, Penicillium frequentence, P. notatum and Botrytis cinerea.  相似文献   

Highly informative genic and genomic SSR markers to facilitate molecular breeding in cultivated groundnut (Arachis hypogaea)     

Manish K. Pandey  Bhimana Gautami  Thandoniappan Jayakumar  Manda Sriswathi  Hari D. Upadhyaya  Makanahally V. Chennabyre Gowda  Thankappan Radhakrishnan  David J. Bertioli  Steven J. Knapp  Duglas R. Cook  Rajeev K. Varshney 《Plant Breeding》2012,131(1):139-147

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Molecular characterization of Blastocystis isolates from children and rhesus monkeys in Kathmandu, Nepal   总被引：1，自引：0，他引：1  

Hisao Yoshikawa  Zhiliang Wu  Kishor Pandey  Basu Dev Pandey  Jeevan Bahadur Sherchand  Tetsuo Yanagi  Hiroji Kanbara 《Veterinary parasitology》2009,160(3-4):295-300

To investigate the possible transmission of Blastocystis organisms between local rhesus monkeys and children in Kathmandu, Nepal, we compared the subtype (ST) and sequence of Blastocystis isolates from children with gastrointestinal symptoms and local rhesus monkeys. Twenty and 10 Blastocystis isolates were established from 82 and 10 fecal samples obtained from children and monkeys, respectively. Subtype analysis with seven sequence-tagged site (STS) primers indicated that the prevalence of Blastocystis sp. ST1, ST2 and ST3 was 20%, 20% and 60% in the child isolates, respectively. In contrast to human isolates, ST3 was not found in monkey isolates and the prevalence of ST1 and ST2 was 50% and 70%, respectively, including three mixed STs1 and 2 and one isolate not amplified by any STS primers, respectively. Since Blastocystis sp. ST2 has been reported as the most dominant genotype in the survey of Blastocystis infection among the various monkey species, sequence comparison of the 150 bp variable region of the small subunit rRNA (SSU rRNA) gene was conducted among ST2 isolates of humans and monkeys. Sequence alignment of 24 clones developed from ST2 isolates of 4 humans and 4 monkeys showed three distinct subgroups, defined as ST2A, ST2B and ST2C. These three subgroups were shared between the child and monkey isolates. These results suggest that the local rhesus monkeys are a possible source of Blastocystis sp. ST2 infection of humans in Kathmandu.  相似文献   

Immunogenic and antigenic properties of recombinant soluble glycoprotein of rabies virus   总被引：2，自引：0，他引：2  

Gupta PK  Sharma S  Walunj SS  Chaturvedi VK  Raut AA  Patial S  Rai A  Pandey KD  Saini M 《Veterinary microbiology》2005,108(3-4):207-214

Rabies virus glycoprotein is a type I transmembrane protein exposed on the surface on the mature virus particle that induces virus neutralizing antibodies. In the present study, 60 amino acid C-terminal hydrophobic anchor (transmembrane) and cytoplasmic domains of glycoprotein were deleted from full-length glycoprotein and fused with polyhistidine tag. The N-terminal viral signal peptide was also replaced with CD33 signal peptide for efficient secretion in mammalian cells. Following transfection of Madin Darby bovine kidney (MDBK) cells with plasmid encoding this soluble form of glycoprotein, polyclonal populations of stably transfected resistant cells were obtained after G418 selection. The protein was expressed as a glycosylated protein and secreted outside the cells utilizing N-terminal CD33 signal peptide. The secreted soluble glycoprotein was purified from cell culture supernatant by Ni--agarose affinity chromatography utilizing C-terminal polyhistidine tag. Like full-length glycoprotein, the expressed recombinant soluble glycoprotein was found to be immunogenic when injected in rabbits. In this study, we have assessed the potential of recombinant soluble glycoprotein as diagnostic antigen in ELISA and found that this recombinant protein can be used as diagnostic antigen in ELISA for detecting anti-glycoprotein antibodies in immunized host.  相似文献   

A questionnaire survey of the management and use of anthelmintics in cattle and antelope in mixed farming systems in Zimbabwe     

Madzingira O  Mukaratirwa S  Pandey VS  Dorny P 《Journal of the South African Veterinary Association》2002,73(2):70-73

A survey of the management of mixed farming of cattle and antelope and use of anthelmintics was conducted on eleven farms between August and December 1999 by a self-administered questionnaire. Seventeen antelope species ranging from grey duikers (Sylvicapra grimmia) to eland (Taurotragus oryx) occurred on the farms. Impala (Aepyceros melampus) was the most abundant antelope on the farms. Seventy-five per cent of the antelope species on the farms were grazers and mixed feeders and shared grazing with cattle. Most farmers (n = 8) did not consider the stocking density for cattle and antelope as an important management factor. Fifty-four per cent of the farmers (n = 6) routinely dewormed both cattle and antelopes. Albendazole and fenbendazole were the most commonly used drugs for deworming cattle (72.7%) and antelope species (54.5%). The deworming of antelope was carried out during the dry season, using albendazole-, fenbendazole-and rafoxanide-medicated supplementary feed blocks. Doramectin injections were given to antelopes on two farms. Cattle were dewormed preventively and according to the general body condition of the animal. Few farmers (n = 4) followed the recommended deworming programme for cattle in Zimbabwe and only one farmer followed a specified dosing programme for game. However, results from the survey on the deworming of game indicate that farmers perceived helminth infections in antelope to be important.  相似文献   

Management ofMeloidogyne incognita inArtemisia pallens with bio-organics     

Rakesh Pandey 《Phytoparasitica》2005,33(3):304-308

The sedentary endoparasitic root-knot nematode [RKN],Meloidogyne incognita (Kofoid and White) Chitwood, is worldwide an economically important agri-pest, reducing the yield and quality of crops. The traditional method of RKN control is based mainly on chemical nematicides, which will not be frequently available from 2005 onwards; their use is highly objectionable due to their major contribution to ground water contamination, and dangers to the environment and to human and animal health. Consequently, new and environmentally safe tools for RKN management are urgently needed. The objective of the present investigation was to evaluate different bio-organic wastes on RKN infestation and the growth / oil yield ofArtemisia pallens Wall. The tested wastes were: 1. Distillation waste ofMentha arvensis (menthol mint); 2.Murraya koengii (meethi neem / curry leaf); 3.Cymbopogon flexuosus (lemongrass); 4.C. martinii (palmarosa); 5.C. winterianus (citronella); 6.Pelargonium graveolens (geranium); 7.Pogostemon patchouli (patchouli); 8.Tagetes minuta (marigold); 9. Vermicompost ofM. arvensis, Chrysanthemum cinerariaefolium (pyrethrum),Tagetes minuta andC. winterianus; 10.Trichoderma harzianum isolate U; 11. VA fungiGlomus aggregatum; G. fasciculatum. The results obtained with these different bio-organics were compared with three controls: untreated — uninoculated, untreated — inoculated and the chemical pesticide carbofuran. Results revealed a considerable enhancement of herbage biomass, flower bud and oil yields when plants were treated with the distillation waste ofC. martinii, C. winterianus, M. koengii, M. arvensis, vermicompost ofC. cinerariaefolium, T. minuta, M. arvensis and bio-agentT. harzianum. Least nematode infections were recorded with the distillation waste ofM. koengii, C. martinii, C. flexuosus, and vermicompost ofT. minuta, C. cinerariaefolium andM. arvensis. The results revealed that these environmentally sound bio-organics could be used for replacement of chemical nematicides. http://www.phytoparasitica.org posting May 20, 2005. The work was conducted at CIMAP-CSIR, Lucknow, India.  相似文献   

Localisation of parasites in the stomach of horses of the region of Settat (Morocco)]     

H Ouhelli  J Cabaret  V S Pandey  A Elkhalfane 《Revue d'élevage et de médecine vétérinaire des pays tropicaux》1979,32(4):347-352

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