Flow cytometric patterns in blood from dogs with non-neoplastic and neoplastic hematologic diseases using double labeling for CD18 and CD45     

Comazzi S  Gelain ME  Spagnolo V  Riondato F  Guglielmino R  Sartorelli P 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2006,35(1):47-54

BACKGROUND: In dogs, flow cytometry is used in the phenotyping of immunologic cells and in the diagnosis of hemic neoplasia. However, the paucity of specific antibodies for myeloid cells and B lymphocytes and of labeled antibodies for multicolor techniques limits the ability to detect all leukocyte subpopulations. This is especially true for neoplastic and precursor cells. CD18 and CD45 are expressed on all leukocytes and are involved in cell activation, and together could be useful in helping determine cell lineage. OBJECTIVES: The purpose of this study was to double label canine blood for CD18 and CD45 and to use the differential expression of antigens to identify leukocyte populations in dogs with non-neoplastic and neoplastic hematologic diseases. METHODS: A template was developed using blood samples from 10 clinically healthy dogs and a back-gating technique. Differential leukocyte counts obtained with the template were compared with those obtained by manual and automated methods on blood samples from 17 additional healthy dogs. Blood samples obtained from 9 dogs with non-neoplastic (reactive) hematologic diseases and 27 dogs with hemic neoplasia were double stained for CD18 and CD45 using mouse anticanine CD18 monoclonal antibody (mAb) plus phycoerythrin-conjugated rat anticanine CD45 mAb and fluorescein isothiocyanate-conjugated rabbit antimouse IgG. Hemic neoplasms were diagnosed by cell morphology, and immunophenotypic and cytochemical markers. RESULTS: With the double label, neutrophils, eosinophils, monocytes, and T- and B-lymphocytes were identified. In reactive disorders, a population of activated neutrophils with high CD45 and CD18 expression was detected. In hemic neoplasia, cell lineage was easily determined, even in acute leukemia. CONCLUSIONS: Double labeling for CD18/CD45 may be useful as a screening method to evaluate hematologic diseases and help determine cell lineage, and to aid in the selection of a panel of antibodies that would be useful for further analysis.  相似文献   

Localisation of iron in wheat grain using high resolution secondary ion mass spectrometry     

Katie L. Moore  Fang-Jie Zhao  Cristina S. Gritsch  Paola Tosi  Malcolm J. Hawkesford  Steve P. McGrath  Peter R. Shewry  Chris R.M. Grovenor 《Journal of Cereal Science》2012

Insufficient iron (Fe) is one of the most prevalent micronutrient deficiencies in humans, with billions of people affected. Cereal grains are an important source of Fe for humans but the bioavailability of Fe in cereals is generally low. Information regarding the cellular and sub-cellular localisation of Fe in wheat grain will aid optimising nutrient delivery for human health. In this study high resolution secondary ion mass spectrometry (NanoSIMS) was used to map the distribution of Fe in the aleurone layer and in the endosperm of immature wheat grain. Iron was shown to be localised strongly in the phytin globoids in the aleurone cells and to a lesser extent in the cytoplasm around the starch granules in the endosperm.  相似文献   

Trafficking and deposition of prolamins in wheat     

Paola Tosi 《Journal of Cereal Science》2012

The processing properties of wheat flour are largely determined by the structures and interactions of the grain storage protein, the so called “gluten proteins”. The synthesis, folding and deposition of the gluten proteins take place within the endomembrane system of the plant cell. Several studies have suggested that two trafficking pathways occur in wheat, with the proteins either being transported via the Golgi apparatus into the vacuole or assembling within the ER to form protein bodies that are subsequently internalized into vacuoles by a process analogous to autophagy. The precise routes of trafficking and deposition of individual gluten proteins remain unclear and so are the molecular and cellular mechanisms regulating their sorting in the two pathways.  相似文献   

Seasonal stability in parasite assemblages of the Brazilian flathead, Percophis brasiliensis (Perciformes: Percophidae): predictable tools for stock identification     

Braicovich PE  Timi JT 《Folia parasitologica》2010,57(3):206-212

A comparison of the composition and structure of parasite communities of the Brazilian flathead, Percophis brasiliensis Quoy et Gaimard (Perciformes: Percophidae) among seasons during one year was carried out in the Argentine Sea. A total of 195 fish specimens were examined and 25 parasite species were found. Parasite communities in seasonal samples showed a high degree of homogeneity in taxonomic composition and infection levels. Similarity analysis showed that the seasonal stability within and between samples was constant in both the composition and community structure throughout the year. Parasites can, therefore, be considered predictable markers for fish stock identification, independently of the season of capture, at least on an annual scale.  相似文献   

Antimycotic effectiveness against dermatophytes: comparison of two in vitro tests     

Galuppi  Roberta  Gambarara  Alessandra  Bonoli  Cristina  Ostanello  Fabio  Tampieri  Maria Paola 《Veterinary research communications》2010,34(1):57-61

Seven antimycotic drugs (econazole, enilconazole, fluconazole, griseofulvin, itraconazole, ketoconazole, and miconazole) were tested against 36 dermatophyte strains (19 M. canis, 7 T. mentagrophytes, 5 M. gypseum, 2 M. cookei, 1 T. rubrum, 1 T. ajelloi, and 1 T. terrestre) isolated from animals, humans, and the environment. Two in vitro methods were compared: a micro-dilution test based on the CLSI M38-A method, and a disk-diffusion test. Fluconazole was not effective in vitro against the dermatophytes. Econazole and enilconazole were the most effective. Thirteen strains were griseofulvin-resistant. The correlation between the two methods was statistically significant for enilconazole, griseofulvin, itraconazole, and miconazole.  相似文献   

Invasive mould infections of the naso-orbital region of cats: a case involving Aspergillus fumigatus and an aetiological review     

Cristina Giordano  Paola Gianella  Stefano Bo  Antonella Vercelli  Chiara Giudice  Daniele Della Santa  Anna Maria Tortorano  Claudio Peruccio  Andrea Peano 《Journal of Feline Medicine and Surgery》2010,12(9):714-723

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Translating land cover/land use classifications to habitat taxonomies for landscape monitoring: a Mediterranean assessment   总被引：1，自引：1，他引：0  

Valeria Tomaselli  Panayotis Dimopoulos  Carmela Marangi  Athanasios S. Kallimanis  Maria Adamo  Cristina Tarantino  Maria Panitsa  Massimo Terzi  Giuseppe Veronico  Francesco Lovergine  Harini Nagendra  Richard Lucas  Paola Mairota  Caspar A. Mücher  Palma Blonda 《Landscape Ecology》2013,28(5):905-930

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Predictors of long-term survival in dogs with high-grade multicentric lymphoma     

Marconato L  Stefanello D  Valenti P  Bonfanti U  Comazzi S  Roccabianca P  Caniatti M  Romanelli G  Massari F  Zini E 《Journal of the American Veterinary Medical Association》2011,239(4):480-485

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What is your diagnosis? Pale yellowish digestive gland and watery tissues in Mediterranean mussels     

Carella F  Aceto S  Maiolino P  De Vico G 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2011,40(2):273-274

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Immune-endocrine interactions in treated and untreated cats naturally infected with FIV     

Gómez NV  Castillo VA  Gisbert MA  Pisano P  Mira G  Fontanals A  Blatter MF 《Veterinary immunology and immunopathology》2011,143(3-4):332-337

Feline immunodeficiency virus (FIV) is a lentivirus that causes a progressive disruption of immune function in cats. The neuroendocrine and immune systems communicate bidirectionally, mediated by cytokines such as tumour necrosis factor-α (TNF), several interleukins (IL-1, IL-6, IL-10), and through signals induced by the ratio of IL-10 to IL-12. FIV can affect both pituitary adrenal and thyroid axis function. Twenty FIV-infected cats in similar stages of the disease were evaluated for six months. A cross-sectional study in which the twenty cats were divided into two groups was performed. Ten were treated with Zidovudine (ZDV: 5mg/kg/d, PO, q12h, for six months) and 10 were untreated. Plasma concentrations of adrenocorticotropic hormone (ACTH), cortisol, T4, FT4, T3, IL-10, IL-12 and viral load (VL) were evaluated after six months. ACTH was found in significantly lower concentrations (p<0.0001) in the treated group whereas cortisol did not show significant differences between the two groups. Both T4 and FT4 had high values in untreated individuals (p<0.001) compared with Zidovudine treated cats. T3 did not show significant differences between the two groups. Both IL-10 and IL-12 were found in significantly higher concentrations in ZDV treated cats (p<0.001). By contrast, the IL10/IL-12 ratio values were significantly lower in untreated cats. Viral load was significantly lower in the treated cats after six months of therapy, compared with values detected pre-treatment (p<0.002). Untreated cats showed a significant increase of VL (p<0.04) compared with the values at the beginning of the study. In treated cats, VL showed lower numbers of viral copies than in untreated cats (p<0.01). In summary, Zidovudine treatment appeared to contribute to the normalization of both the adrenal and thyroid axes. This effect could be attributed to the decrease observed in VL, resulting in a change in cytokine patterns.  相似文献