MicroRNA expression in the cerebrospinal fluid of dogs with and without cervical spondylomyelopathy     

Daniella P. Vansteenkiste  Joelle M. Fenger  Paolo Fadda  Paula Martin‐Vaquero  Ronaldo C. da Costa 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(6):2685-2692

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A sonographic investigation for the development of ultrasound-guided paravertebral brachial plexus block in dogs: cadaveric study     

Paolo Monticelli  Ella Fitzgerald  Jaime Viscasillas 《Veterinary anaesthesia and analgesia》2018,45(2):195-202

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The effects of starter microbiota and the early life feeding of medium chain triglycerides on the gastric transcriptome profile of 2-or 3-week-old cesarean delivered piglets     

Paolo Trevisi  Davide Priori  Vincenzo Motta  Diana Luise  Alfons J.M.Jansman  Sietse-Jan Koopmans  Paolo Bosi 《畜牧与生物技术杂志(英文版)》2018,(1)

Background: The stomach is an underestimated key interface between the ingesta and the digestive system, affecting the digestion and playing an important role in several endocrine functions. The quality of starter microbiota and the early life feeding of medium chain triglycerides may affect porcine gastric maturation. Two trials(T1, T2) were carried out on 12 and 24 cesarean-delivered piglets(birth, d0), divided over two microbiota treatments, but slaughtered and sampled at two or three weeks of age, respectively. All piglets were fed orally: sow serum(T1) or pasteurized sow colostrum(T2) on d0; simple starter microbiota(Lactobacillus amylovorus, Clostridium glycolicum and Parabacteroides spp.)(d1-d3); complex microbiota inoculum(sow diluted feces, CA) or a placebo(simple association, SA)(d3-d4) and milk replacer ad libitum(d0-d4). The The T1 piglets and half of the T2 piglets were then fed a moist diet(CTRL); the remaining half of the T2 piglets were fed the CTRL diet fortified with medium chain triglycerides and 7% coconut oil(MCT). Total m RNA from the oxyntic mucosa was analyzed using Affymetrix?Porcine Gene array strips. Exploratory functional analysis of the resulting values was carried out using Gene Set Enrichment Analysis.Results: Complex microbiota upregulated 11 gene sets in piglets of each age group vs. SA. Of these sets, 6 were upregulated at both ages, including the set of gene markers of oxyntic mucosa. In comparison with the piglets receiving SA, the CA enriched the genes in the sets related to interferon response when the CTRL diet was given while the same sets were impoverished by CA with the MCT diet.Conclusions: Early colonization with a complex starter microbiota promoted the functional maturation of the oxyntic mucosa in an age-dependent manner. The dietary fatty acid source may have affected the recruitment and the maturation of the immune cells, particularly when the piglets were early associated with a simplified starter microbiota.  相似文献   

The effect of urban refuse compost and different tractors tyres on soil physical properties, soil erosion and maize yield     

Paolo Bazzoffi  Sergio Pellegrini  Andrea Rocchini  Miranda Morandi  Olga Grasselli 《Soil & Tillage Research》1998,48(4):918-286

Application of urban refuse compost to agricultural soil could help to solve municipalities' problems related to the increasing production of waste only if soil property improvement and environmental conservation can be demonstrated. The use of low-pressure tractor tyres is another proposal in modern agriculture for reducing soil compaction. This study thus aimed to detect the effects of both compost and low-pressure tractor tyres on soil loss, runoff, aggregate stability, bulk density, penetrometer resistance and maize (Zea mays L.) yield. A 3-year field experiment was carried out on a hilly (15% slope) clay loam soil in central Italy. Twelve plots (200 m² each) were monitored with tipping-pot devices for runoff and soil erosion measurement. Treatments were: compost addition (64 Mg ha⁻¹), mineral fertilisation, use of low-pressure tyres, use of traditional tyres, with three replicates, in a fully randomised block design. Compost was applied once at the beginning of the experiment. Runoff reduction due to compost ranged between 7 and 399 m³ ha⁻¹ during seasons, while soil erosion was reduced between 0.2 and 2.4 Mg ha⁻¹. Mean weight diameter (MWD) of stable aggregates, measured on wheel tracks, increased by 2.19 mm, then progressively decreased. Compost significantly increased bulk density by 0.08 Mg m⁻³ due to its inert fraction content. This effect was less evident in the second and third year, probably due to harrowing. Maize yields were slightly, but significantly, reduced in composted plots by 1.72 Mg ha⁻¹ in the third year. Low-pressure tyres significantly reduced soil loss in the third year by 1 Mg ha⁻¹. Furthermore, they did not significantly influence runoff volumes and soil structural stability. Low-pressure tyres or compost addition were singly able to prevent an increase in penetrometer resistance due to agricultural machinery traffic. Low-pressure tyres increased the maize yield during the 3 years and the difference (0.4 Mg ha⁻¹) became significant in the third year. In conclusion, results show the positive lasting effect of compost in ameliorating soil physical properties and reducing runoff and soil erosion. Low-pressure tyres appear justifiable both for the observed increase of grain production and reduction of soil compaction. This latter effect is, nevertheless, masked by compost addition which is also able to reduce penetrometer resistance. Further research is required to explain the causes of the slight inhibition of grain yield observed when compost was compared with mineral fertilisation.  相似文献   

Intracellular and extracellular enzyme activity in soil with reference to elemental cycling     

Oliver Duly  Paolo Nannipieri 《植物养料与土壤学杂志》1998,161(3):243-248

Enzyme activities play an important role for the transformation of elements and compounds in soil and, thus, were extensively analyzed for more than 4 decades. The activity of any enzyme in soil may not only be controlled by active organisms. Substantial parts of ‘extracellular’ enzymes may be stabilized by abiotic soil components maintaining their activity. Methods to discriminate the source of enzyme activity were summarized with emphasis on the approach plotting enzyme activity versus a feature integrating the microbial biomass after the addition of glucose and nitrate. Considering the quotient between enzyme activity and microbial biomass content, protease activity will be discussed with reference to nitrogen transformation in soils.  相似文献   

Use of macroalgae supplemented with probiotics in the Haliotis rufescens (Swainson, 1822) culture in Northern Chile     

Fernando R Silva‐Aciares  Paolo O Carvajal  Cristian A Mejías  Carlos E Riquelme 《Aquaculture Research》2011,42(7):953-961

In this study, we evaluate the use of macroalgae as vectors of probiotics bacteria into the digestive tract of abalone to improve their survival and growth. It is shown that when abalone Haliotis rufescens of different sizes were fed with a natural diet composed of fronds of the macroalga Macrocystis integrifolia supplemented with a mixture of Vibrio sp. C21‐UMA, Agarivorans albus F1‐UMA and Vibrio sp. F15‐UMA bacteria, there was a significant increase (P<0.05) in the average monthly growth rate and survival (%)in a period of 210 days, compared with the control without a probiotic supplement. The permanence of the probiotics in the digestive tract of the animals was monitored, and it was found that the number of culturable C21‐UMA and F1‐UMA bacteria decreased significantly (P<0.05) in recently weaned and adult abalone, and they almost disappeared completely on day 19 of the bioassay. However, the culturable Vibrio sp. F15‐UMA disappeared completely from the digestive tract on day 22 of the bioassay, and these were the bacteria that remained at the highest concentration compared with the other two bacterial strains over the experimental period. It is therefore shown that it is feasible to use a probiotic mixture to improve the profitability of the H. rufescens culture.  相似文献   

Sea turtle by‐catch in the Mediterranean     

Paolo Casale 《Fish and Fisheries》2011,12(3):299-316

Sea turtle by‐catch data in the Mediterranean were reviewed and analysed with fishing effort. The results indicate over 132 000 captures per year, with probably over 44 000 incidental deaths per year, while many others are killed intentionally. Small vessels using set net, demersal longline or pelagic longline represent most of the Mediterranean fleet and likely cause more incidental or intentional deaths than large vessels typically using bottom trawl or pelagic longline. When interactions, mortality, intentional killing, size (a proxy for reproductive value) and turtle populations are considered, results indicate that Mediterranean green (Chelonia mydas) and loggerhead turtles (Caretta caretta) are more affected (i) by fishing gears such as bottom trawlers, demersal longlines and set nets, (ii) by small‐scale fisheries, and (iii) by fishing in the eastern basin. Although small‐scale fisheries should be the priority target, available measures are easier to implement on the fewer large vessels. Moreover, these measures are few, and they are not implemented yet, while others should still be tested for the Mediterranean fisheries. Thus, measures for reducing captures or mortality through changing gear‐specific characteristics may help, but probably a more holistic conservation strategy aimed to an ecosystem‐based fishery management for a sustainable fishing would be the only solution for the long‐term survival of Mediterranean Sea turtle populations and their habitats. Small‐scale fisheries should manage marine resources, including turtles, in a responsible and sustainable way. Turtles may not only benefit from but can also help this process if their non‐consumptive value is fully recognized.  相似文献   

Growth and feed utilization of gilthead sea bream (<Emphasis Type="Italic">Sparus aurata</Emphasis>, L.) fed to satiation and restrictively at increasing dietary energy levels     

Alessio Bonaldo  Gloria Isani  Ramon Fontanillas  Luca Parma  Ester Grilli  Pier Paolo Gatta 《Aquaculture International》2010,18(5):909-919

Three isoproteic (47% protein) diets were formulated to contain graded levels of crude fat (diet D16:16%, diet D24: 24% and diet D32: 32%). Each diet was fed to satiation in three and to 80% satiation in two replicate groups of gilthead sea bream (Sparus aurata), having an initial body weight of 72–74 g. The trial lasted 81 days. Groups fed to satiation showed higher final body weight (FBW; 238.8–252.3 g vs. 218.0–229.3 g) and daily growth index (DGI; 2.49–2.65%/day vs. 2.27–2.34%/day) than those fed to 80% satiation. Feed intake was significantly different both for feeding level and for diet composition. Fish fed to satiation had higher feed conversion rate (FCR) compared to the 80% satiation groups (1.33–1.44 vs. 1.13–1.17; P ≤ 0.001). Within satiation groups, FCR was significantly lower in fish fed D16 compared to fish fed D32 (1.33 vs. 1.44, P ≤ 0.05), whereas no statistical differences were found within the 80% satiation groups. The increase in dietary lipid level did not improve growth performance, feed efficiency and protein utilization but decreased gross lipid efficiency. Conversely, a reduction in ration from satiation to 80% satiation decreased DGI, thus improving FCR. Feed costs were influenced by dietary energy level and feeding ratio, the lowest energy diet at 80% satiation being the most profitable combination among the variables.  相似文献   

Sea turtle strandings reveal high anthropogenic mortality in Italian waters     

Paolo Casale  Marco Affronte  Gianni Insacco  Daniela Freggi  Carola Vallini  Paola Pino d'Astore  Roberto Basso  Giuseppe Paolillo  Graziana Abbate  Roberto Argano 《水产资源保护:海洋与淡水生态系统》2010,20(6):611-620

• 1. Spatio‐temporal distribution and anthropogenic mortality factors were investigated in loggerhead turtles (Caretta caretta) found stranded or floating in the waters around Italy. A total of 5938 records for the period 1980–2008 were analysed concerning loggerhead turtles measuring from 3.8 to 97 cm curved carapace length (mean: 48.3 cm).
• 2. Results highlighted the following conservation issues: (i) in the study area, anthropogenic mortality is higher than natural mortality; (ii) interaction with fisheries is by far the most important anthropogenic mortality factor; (iii) longlines are an important mortality factor in the southern areas; (iv) trawlers are the cause of high numbers of dead strandings in the north Adriatic; (v) entanglement in ghost‐gear or in other anthropogenic debris affects high numbers of turtles; and (vi) boat strikes are an important source of mortality in most areas but mostly in the warm seasons.
• 3. Results also indicate that: (vii) the north Adriatic is the area with the highest turtle density; and (viii) the south Adriatic and to a lesser extent the surrounding areas of the north Adriatic and the Ionian, are important developmental areas for loggerhead turtles in the first years of life.
• 4. Italy is in the centre of the Mediterranean Sea and borders major foraging areas for the loggerhead turtles in the region, and these results confirm previous concerns about the level of anthropogenic mortality in Italian waters. Copyright © 2010 John Wiley & Sons, Ltd.

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Mortality outbreak by perch rhabdovirus in European perch (Perca fluviatilis) farmed in Italy: Clinical presentation and phylogenetic analysis     

Claudio Caruso  Andrea Gustinelli  Paolo Pastorino  Pier Luigi Acutis  Riccardo Prato  Loretta Masoero  Simone Peletto  Maria Letizia Fioravanti  Marino Prearo 《Journal of fish diseases》2019,42(5):773-776

This work reports a mortality outbreak, occurred in 2015 and affecting juveniles of European perch (Perca fluviatilis L.) farmed in Italy. Perch rhabdovirus (PRV) was detected by viral isolation and biomolecular investigations. Phylogenetic analysis clustered our isolate into genogroup B, which also includes PRV isolates from Perca fluviatilis identified in France (2004–2009); diagnostic investigations also revealed opportunistic bacteria (Aeromonas hydrophila) and parasites (Chilodonella piscicola). Since, occasionally, PRV has been reported in the natural environment, which is often a source of eggs and broodstock for farms, it could be possible that both similar France and Italian isolate were imported from a same place elsewhere and have a common origin. Improving biosecurity measures (batch control) and disinfection of egg strings with an iodine‐based solution helps prevent apparent vertical transmission of PRV.  相似文献