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101.
102.
Over the past century, trees have encroached into grass- and shrublands across western North America. These include Douglas-fir trees (Pseudotsuga menziesii (Mirb.) Franco var. glauca (Beissn.) Franco) encroaching into mountain big sagebrush (Artemisia tridentata Nutt. ssp. vaseyana (Rydb.) Beetle) from stable islands of savanna in southwestern Montana. Our objectives were to quantify the relative area occupied by mountain big sagebrush and grasslands versus Douglas-fir savanna in the Fleecer Mountains of southwestern Montana today and in the past, and to identify the historical role of fire and other factors in maintaining this distribution. To do this, we reconstructed a multicentury history of tree establishment and surface fires from 1120 trees sampled on a grid of 50 plots covering 1030 ha of a modern mosaic of sagebrush–grasslands and Douglas-fir trees. We compared these histories to time series of climate and land use, and to spatial variation in topography and soil moisture availability. Beginning in the mid-1800s, Douglas-fir trees established in areas in which trees did not persist historically. In 1855, less than half the plots (42%) had trees whereas today, most plots do (94%). This encroachment was synchronous with the cessation of frequent surface fires, likely caused by the advent of domestic livestock grazing, and perhaps by the start of several decades of relatively dry summers. Douglas-fir savannas historically occurred on fire-safe sites more often than did sagebrush–grass. Our inference that frequent fire excluded Douglas-fir in the past was supported by the results of a simulation model of fire and associated vegetation dynamics. In the continued absence of fire, mountain big sagebrush and grasslands in southwestern Montana are likely to become more homogeneous as Douglas-fir trees continue to encroach.  相似文献   
103.
Risk of Campylobacter infection in humans has been associated with many sources, including dogs. C. upsaliensis is the most common species found in canines, and has been occasionally isolated from symptomatic humans. This study aimed to investigate the genetic diversity of 41 C. upsaliensis isolates carried by dogs and from nine isolates carried by humans using Multilocus sequence typing (MLST). We identified considerable genetic diversity amongst the C. upsaliensis isolates from both dogs and humans, identifying 45 different sequence types (STs). All STs were new, apart from that of the reference strain. Only three STs were found in more than one isolate: ST-72 (2 isolates), ST-98 (2 isolates) and ST-104 (3 isolates). ST-104 was the only ST to be encountered in both dogs and humans. Thirty-one of the 45 STs were assigned to one of 13 clonal complexes (CCs). Four of these CCs contained STs originating from both humans and dogs. None of the CCs contained exclusively human isolates, and two isolates from dogs within the same kennel belonged to the same CC. The large amount of diversity found in both dog and human isolates of C. upsaliensis, combined with the relatively small database, made it difficult to assign strains to sources of infection. This emphasizes the need to increase the size of the database. Dog and human isolates occasionally grouped together, however there were insufficient human-derived isolates to determine whether or not dogs are a common source of infection. Although C. upsaliensis infection is rare in humans, dogs still remain a potential source, and are therefore a possible zoonotic risk. Further work is needed to investigate the epidemiology of C. upsaliensis infection in humans.  相似文献   
104.
The objectives of this study were to determine the chemical composition and nutritive value of corn protein product 1 (CPP 1), corn protein product 2 (CPP 2), and corn fiber (CF), novel coproducts of the ethanol industry, and compare these feed ingredients with standard plant protein ingredients [soybean meal (SBM), distillers dried grains with solubles (DDGS), corn gluten meal (CGlM), and corn germ meal (CGeM)], and to compare CF sources (CF control 1 and control 2) with standard fiber sources (peanut hulls, Solka-Floc, and beet pulp) commonly used in pet foods. Corn fiber, CPP 1, and CPP 2 were produced at a pilot-scale modified dry-grind plant, with CPP 2 having a greater degree of purification than CPP 1. Crude protein values for CPP 2 and CPP 1 were 57.3 and 49.7%, respectively. Total dietary fiber concentration was 29% for CPP 2 and 23.5% for CPP 1. Acid-hydrolyzed fat and GE concentrations were similar for these ingredients. In a protein efficiency ratio assay, no differences (P > 0.05) in feed intake, BW gain, or CP intake were noted for CPP 2, CPP 1, or CGlM. However, feeding CPP 2 resulted in a greater (P < 0.05) G:F ratio and protein efficiency ratio than CPP 1 and CGlM. In a cecectomized rooster assay, CGlM had numerically the greatest standardized total AA, total essential AA, and total nonessential AA digestibilities, but they were not different (P > 0.05) from CPP 1 or SBM values. Corn germ meal resulted in the least values, but they were not different from those for DDGS and CPP 1. The greatest values for true nitrogen-corrected ME were obtained with CGlM, followed by CPP 2, DDGS, CPP 1, SBM, and CGeM. Distillers dried grains with solubles and CPP 1 had similar true nitrogen-corrected ME values, and they were not different from values for CPP 2 and SBM. In vitro CP disappearance was greatest (P < 0.05) for CGlM (94.1%), intermediate for DDGS (76.8%) and CPP 1 (77.5%), and least for CPP 2 (74.1%) and CGeM (67.7%). Corn fibers contained predominantly insoluble dietary fiber (1% or less of soluble dietary fiber), with a moderate CP concentration. In vitro OM disappearance for the fiber sources, when using inoculum from dog feces, revealed that with the exception of beet pulp, which had a moderate disappearance value after 16 h of fermentation (17.7%), all fiber substrates had a nonsignificant extent of fermentation. In conclusion, novel corn coproducts had properties comparable with the standard protein and fiber sources used in animal nutrition.  相似文献   
105.
Fusarium ear rot, primarily caused by Fusarium verticillioides, causes losses in grain yield and quality and can result in contamination of grain by mycotoxins, primarily fumonisin B1. Disease severity and fumonisin B1 contamination vary considerably among maize‐growing regions and from year to year. A 2 year field study was conducted in six locations in the USA, to evaluate the roles of planting date, maize hybrid, rainfall, temperature and insect pests in the variation in fusarium ear rot symptoms and fumonisin B1 contamination. Grain samples were inspected to determine percentage of kernels with fusarium ear rot symptoms, categorized as ‘moulded’ or ‘starburst’; grain was also analysed by ELISA for fumonisin B1. Hybrid and planting date frequently had significant effects (P ≤ 0·05) on fusarium ear rot and fumonisin B1 contamination. Earlier planting consistently resulted in lower ear rot severity, fumonisin B1 levels and insect damage. Mould symptoms were highly correlated with thrips populations (Frankliniella occidentalis) (r = 0·78) and with fumonisin B1 concentration (r = 0·89). The starburst symptom was not as closely correlated with thrips (r = 0·33) or fumonisin B1 (r = 0·18). A multiple linear regression model identified highly significant effects on fumonisin B1 for thrips, lower average daily precipitation after flowering, and location. These results strengthen the evidence that locations with high populations of ear‐infesting thrips and dry conditions after pollination have an increased risk of fumonisin contamination, and suggest that high fumonisin levels are much more likely in visibly moulded kernels compared to those with ‘starburst’ symptoms.  相似文献   
106.
Distributing animals from a single breeding program to a global market may not satisfy all producers, as they may differ in market objectives and farming environments. Analytic hierarchy process (AHP) is used to estimate preferences, which can be aggregated to consensus preference values using weighted goal programming (WGP). The aim of this study was to use an AHP-WGP based approach to derive desired genetic gains for rainbow trout breeding and to study whether breeding trait preferences vary depending on commercial products and farming environments. Two questionnaires were sent out. Questionnaire-A (Q-A) was distributed to 178 farmers from 5 continents and used to collect information on commercial products and farming environments. In this questionnaire, farmers were asked to rank the 6 most important traits for genetic improvement from a list of 13 traits. Questionnaire B (Q-B) was sent to all farmers who responded to Q-A (53 in total). For Q-B, preferences of the 6 traits were obtained using pairwise comparison. Preference intensity was given to quantify (in % of a trait mean; G%) the degree to which 1 trait is preferred over the other. Individual preferences, social preferences, and consensus preferences (Con-P) were estimated using AHP and WGP. Desired gains were constructed by multiplying Con-P by G%. The analysis revealed that the 6 most important traits were thermal growth coefficient (TGC), survival (Surv), feed conversion ratio (FCR), condition factor (CF), fillet percentage (FIL%), and late maturation (LMat). Ranking of traits based on average Con-P values were Surv (0.271), FCR (0.246), TGC (0.246), LMat (0.090), FIL% (0.081), and CF (0.067). Corresponding desired genetic gains (in % of trait mean) were 1.63, 1.87, 1.67, 1.29, 0.06, and 0.33%, respectively. The results from Con-P values show that trait preferences may vary for different types of commercial production or farming environments. This study demonstrated that combination of AHP and WGP can be used to derive desired gains for a breeding program and to quantify differences due to variations market demand or production environment.  相似文献   
107.
Objective : To review the postoperative results and complications associated with urethral sphincter mechanism incompetence managed with a static hydraulic urethral sphincter. Methods : Case records and a telephone owner questionnaire were retrospectively used to assess postoperative urinary continence scores (1 – dripping constantly to 10 – completely dry) and presence and frequency of complications. Results : Eleven spayed females were included. Median continence score/10 (range) awarded preoperatively was 3 (2 to 6), and scores at two weeks, three and six months were 8 (4 to 10), 9 (4 to 10) and 8 (4 to 10), respectively. At the last survey, the median continence score of 9 (5 to 10) was significantly better (P=0·004) than before surgery. Complete continence was achieved in 36·4% of dogs. The median (range) follow‐up time was 412 (118 to 749) days. Complications occurred in 9 of 11 dogs and included dysuria (n=7), bacterial cystitis (n=7), longer urination time (n=8), urinary retention (n=3), haematuria (n=1), pain (n=3) and incisional seroma (n=3). Clinical Significance : Static hydraulic urethral sphincter was frequently associated with minor complications but no major complications (i.e. those requiring further surgery). Continence scores were significantly improved compared with those before surgery, with the possibility of further improvement following inflation of the sphincter.  相似文献   
108.
109.
Three adult horses were evaluated for signs of musculoskeletal pain, dullness, ataxia, and seizures. A diagnosis of bacterial meningitis was made on the basis of results of CSF analysis. Because primary bacterial meningitis is so rare in adult horses without any history of generalized sepsis or trauma, immune function testing was pursued. Flow cytometric phenotyping of peripheral blood lymphocytes was performed, and proliferation of peripheral blood lymphocytes in response to concanavalin A, phytohemagglutinin, pokeweed mitogen, and lipopolysaccharide was determined. Serum IgA, IgM, and IgG concentrations were measured by means of radial immunodiffusion, and serum concentrations of IgG isotypes were assessed with a capture antibody ELISA. Serum tetanus antibody concentrations were measured before and 1 month after tetanus toxoid administration. Phagocytosis and oxidative burst activity of isolated peripheral blood phagocytes were evaluated by means of simultaneous flow cytometric analysis. Persistent B-cell lymphopenia, hypogammaglobulinemia, and abnormal in vitro responses to mitogens were detected in all 3 horses, and a diagnosis of common variable immunodeficiency was made.  相似文献   
110.
(1) Four chick experiments were conducted to determine toxicity estimates for basic zinc chloride (BZC) and basic copper chloride (BCC), now being used as sources for these minerals. (2) In experiment 1, New Hampshire x Columbian crossbred chicks were fed 0, 500, 1000, 1500, 3000 and 5000 mg Zn/kg from BZC (Zn5Cl2(OH)8). Broken-line regression analysis showed that the minimal toxic break points for chick weight gain and gain:food were 1720 and 2115 mg Zn/kg, respectively. (3) Crossbred chicks were fed 0, 150, 250, 500, 750 and 1000 mg Cu/kg from BCC (Cu2(OH)3C) in experiment 2. Regression analysis indicated that the minimal toxic break points for chick weight gain and gain:food were 642 and 781 mg Cu/kg, respectively. (4) In experiment 3, commercial broiler chicks were fed 0, 1500, 2000 and 2500 mg supplemental Zn/kg from BZC or 0, 500, 650 and 800 mg supplemental Cu/kg from BCC. Broiler chicks fed those high inclusion rates of Zn did not show reduced weight gain in comparison to chicks fed no supplemental Zn. All high concentrations of supplemental Cu depressed chick weight gain in comparison to control chicks. (5) Experiment 4 involved two separate 4 x 2 factorial designs with supplemental Zn (0, 2500, 3500 and 4500 mg/kg) or Cu (0, 500, 750 and 1000 mg/kg) and two breeds of chicks (crossbred and commercial). Significant interactions for weight gain, food intake, gain:food and liver Cu suggested that the crossbred and commercial chicks responded differently to high concentrations of supplementary dietary Cu.  相似文献   
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