Ganglioneuroblastoma in a cat: a rare neoplasm treated with electrochemotherapy     

Spugnini EP  Citro G  Dotsinsky I  Mudrov N  Mellone P  Baldi A 《Veterinary journal (London, England : 1997)》2008,178(2):291-293

An 8-year-old male castrated cat was referred for sudden onset of lameness. Physical examination revealed a 1x2x1cm mass originating from a footpad of the right hind leg. A diagnosis of ganglioneuroblastoma was suggested by the tumour appearance following histopathological staining with haematoxylin and eosin and haematoxylin/van Gieson. Immunohistochemical staining for glial fibrillary acidic protein (GFAP), vimentin, neuron-specific enolase (NSE), neurofilament and S100 further confirmed the diagnosis. The staging process did not indicate metastatic spread. The cat was treated with three sessions of electrochemotherapy (ECT) 1 week apart, following local injection of bleomycin. The tumour had completely regressed within 1 week of the third ECT application and remained in remission for 402 days at which time a small recurrence was noted. The animal was given a further session of ECT using intra-lesional cisplatin and again went into remission. It remained tumour free at 450 days. Electrochemotherapy is considered a safe and effective treatment for localised neoplasms of cats and dogs and warrants further investigation.  相似文献   

Biochemical responses to iron deficiency in kiwifruit (Actinidia deliciosa)     

Rombolà AD  Brüggemann W  López-Millán AF  Tagliavini M  Abadía J  Marangoni B  Moog PR 《Tree physiology》2002,22(12):869-875

A comparative study of two kiwifruit genotypes (Actinidia deliciosa (A. Chev.) C.F. Liang et A.R. Ferguson var. deliciosa) with different tolerance to iron (Fe) deficiency was conducted to identify biochemical features associated with tolerance to Fe deficiency. After 14 days of growth in hydroponic culture under Fe-deficient and Fe-sufficient conditions, leaf chlorophyll concentration, activities of ferric chelate reductase (FCR), phosphoenolpyruvate carboxylase (PEPC) and citrate synthase in root extracts, concentrations of organic acids in roots, leaves and xylem sap, and xylem sap pH were measured. In response to Fe deficiency, the tolerant genotype D1 showed: (i) higher FCR activity associated with a longer lasting induction of FCR; (ii) higher PEPC activity; (iii) higher concentrations of citric acid in roots; and (iv) lower xylem sap pH compared with the susceptible genotype Hayward. These findings imply that induction of FCR and PEPC activities in roots in response to Fe deficiency are important physiological adaptations enabling Fe-efficient kiwifruit plants to tolerate Fe deficiency.  相似文献   

Soil Conservation in Europe: Wish or Reality?     

Panos Panagos  Anton Imeson  Katrin Meusburger  Pasquale Borrelli  Jean Poesen  Christine Alewell 《Land Degradation \u0026amp; Development》2016,27(6):1547-1551

Nearly all of Europe is affected by soil erosion. A major policy response is required to reverse the impacts of erosion in degraded areas, particularly in light of the current climate change and water crisis. Soil loss occurs not because of any lack of knowledge on how to protect soils, but a lack in policy governance. The average rate of soil loss by sheet and rill erosion in Europe is 2·46 Mg ha⁻¹ yr⁻¹. To mitigate the impacts of soil erosion, the European Union's Common Agricultural Policy has introduced conservation measures which reduce soil loss by water erosion by 20% in arable lands. Further economic and political action should rebrand the value of soil as part of ecosystem services, increase the income of rural land owners, involve young farmers and organize regional services for licensing land use changes. In a changing World of 9 billion people with the challenge of climate change, water scarcity and depletion of soil fertility, the agriculture economy should evolve taking into account environmental and ecological aspects. © 2016 The Authors Journal of Land Degradation & Development Published by John Wiley & Sons Ltd.  相似文献   

Transport stress in horses: Effects of two different distances     

Alessandra Tateo  Barbara Padalino  Marianna Boccaccio  Aristide Maggiolino  Pasquale Centoducati 《Journal of Veterinary Behavior: Clinical Applications and Research》2012,7(1):33-42

This study compares the effects of 2 journeys of different lengths (50 km and 200 km) on horses. The first journey lasted about 1 hour, the second about 3 hours. Twelve Standardbred horses were used for the test. Blood samples were collected and analyzed (packed cells volume [PCV], cortisol, aspartate transaminase, alanine transaminase, creatine kinase, lactate dehydrogenase, creatinine, glucose, triglycerides, cholesterol, blood urea nitrogen, calcium, phosphorus, chlorine, total proteins, albumin, and alkaline phosphatase), and heart rate (HR), respiratory rate, and temperature were measured at 5 different experimental times: at rest, at loading, at unloading, and at 2 and 4 hours after return from the journey. Behavior was recorded by direct observation of loading and unloading, within the vehicle, and 2 and 4 hours after arrival in a new stall. The same parameters were recorded at the same times (excluding loading and unloading) in a control group of horses that had not travelled. All the data were analyzed using a repeated measures analysis (analysis of variance).Loading always caused an increase of HR and PCV. The different journey lengths produced significant increases in serum glucose, cortisol concentration, PCV, and HR. Serum cortisol concentrations after short transport were considerably higher than values registered at rest and after long transport. The long journey caused an increase of creatine kinase 2 hours after return. On arrival in the new stalls, the horses that had travelled sniffed less and snorted later than the control group. They ate immediately and more quickly, and those that had travelled 200 km also showed more interest in water than those that travelled for 50 km and the control group. The other behaviors, including exploratory, rest, and play activities, were concentrated in the second 2-hour observation period when the horses tended to feed less. The present trial showed that adaptation to a new stall could be facilitated by offering horses food and fresh water when they arrive, and that for horses that must make a 3-hour journey before a race, it would be better to arrive at their destination at least 4 hours before beginning physical activity.  相似文献   

Molecular basis of the interaction between proteins of plant origin and proanthocyanidins in a model wine system     

Granato TM  Piano F  Nasi A  Ferranti P  Iametti S  Bonomi F 《Journal of agricultural and food chemistry》2010,58(22):11969-11976

Plant proteins are being used as a replacement for animal proteins in wine fining. The surface hydrophobicity of plant proteins in four commercial preparations differing for their origin and processing was assessed by using a fluorescent hydrophobic probe in wine-like media. Displacement of the probe by addition of wine phenolics was measured as a way to compare and predict to some extent the efficiency of these proteins in wine fining. It was found that the binding of polyphenols was much more specific than that of the hydrophobic probe. Further analysis of the polyphenol pattern in protein-treated wine-like solutions pointed out two relevant facts: (1) proteins may interfere with the chemistry of the interactions between polyphenols and other wine components; (2) individual protein preparation having different surface hydrophobicities also have different specificities in binding different polymeric forms of the polyphenols and in their substitution products. These findings are related to the possible carry-over of transition metals and may be worth exploring for custom tailoring the fining process. Whether the practical application of the latter finding will call for production and/or screening of plant-derived proteins with features appropriate to this task remains to be investigated. However, the approaches presented in this study may be used for large-scale screening of protein suitability for fining application under laboratory conditions, providing guidelines for their use in actual winemaking applications.  相似文献   

Inducible Expression of a Phytolacca heterotepala Ribosome-Inactivating Protein Leads to Enhanced Resistance Against Major Fungal Pathogens in Tobacco     

Corrado G  Bovi PD  Ciliento R  Gaudio L  Di Maro A  Aceto S  Lorito M  Rao R 《Phytopathology》2005,95(2):206-215

  相似文献   

Effect of nutritive level on carcass traits and meat quality of IHDH foals     

Pasquale De Palo  Alessandra Tateo  Aristide Maggiolino  Pasquale Centoducati 《Animal Science Journal》2014,85(7):780-786

The present work describes the effect of nutritive level on horse carcass traits and on meat quality. Eighteen male Italian Heavy Draught Horse (IHDH) breed foals were employed in the study. Soon after foaling they were randomly subdivided into three groups according to three nutritive level classes: 150%, 180% and 200% of maintenance requirements. Live weight, hot carcass weight and dressing percentage of each animal were recorded. After slaughtering, meat samples were collected from Longissimus dorsi muscle. The right half carcass of each animal was then divided into cuts. Each one was subdivided into lean, fat and bones. Live weight, carcass weight and dressing percentage were not affected by nutritive level (P > 0.05). Horses fed with the lower nutritive level showed a higher incidence of lean and a lower incidence of fat (P < 0.01). Moreover, fatty acid profile was not affected by nutritive level (P > 0.05). Probably the tendency of IHDH foals to concentrate adipogenesis in the subcutaneous district could explain the lack of influence of nutritive level on meat quality parameters and its influence on carcass and cut composition, which tend to be richer in fat.  相似文献   

Smart textiles biotechnology for electrocardiogram monitoring in horses during exercise on treadmill: Validation tests     

Martina Felici  Mimma Nardelli  Antonio Lanatà  Micaela Sgorbini  Enzo Pasquale Scilingo  Paolo Baragli 《Equine veterinary journal》2021,53(2):373-378

  相似文献   

Genotype-specific changes associated to early synthesis of autotetraploids in wild potato species     

Riccardo Aversano  Maria-Teresa Scarano  Giovanna Aronne  Immacolata Caruso  Vincenzo D’Amelia  Veronica De Micco  Carlo Fasano  Pasquale Termolino  Domenico Carputo 《Euphytica》2015,202(2):307-316

  相似文献   

Biotechnological production of vitamin B2-enriched bread and pasta     

Capozzi V  Menga V  Digesu AM  De Vita P  van Sinderen D  Cattivelli L  Fares C  Spano G 《Journal of agricultural and food chemistry》2011,59(14):8013-8020

Lactic acid bacteria (LAB) were obtained from durum wheat flour samples and screened for roseoflavin-resistant variants to isolate natural riboflavin-overproducing strains. Two riboflavin-overproducing strains of Lactobacillus plantarum isolated as described above were used for the preparation of bread (by means of sourdough fermentation) and pasta (using a prefermentation step) to enhance their vitamin B2 content. Pasta was produced from a monovarietal semolina obtained from the durum wheat cultivar PR22D89 and, for experimental purposes, from a commercial remilled semolina. Several samples were collected during the pasta-making process (dough, extruded, dried, and cooked pasta) and tested for their riboflavin content by a high-performance liquid chromatography method. The applied approaches resulted in a considerable increase of vitamin B2 content (about 2- and 3-fold increases in pasta and bread, respectively), thus representing a convenient and efficient food-grade biotechnological application for the production of vitamin B2-enriched bread and pasta. This methodology may be extended to a wide range of cereal-based foods, feed, and beverages. Additionally, this work exemplifies the production of a functional food by a novel biotechnological exploitation of LAB in pasta-making.  相似文献