Investigation into the potential pathogenicity of Staphylococcus felis in a cat     

Patel A  Lloyd DH  Howell SA  Noble WC 《The Veterinary record》2002,150(21):668-669

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Tissue culture micropropagation of Douglas-fir   总被引：1，自引：0，他引：1  

Gina H. Mohammed  Kamlesh R. Patel 《New Forests》1989,3(2):125-139

A procedure was developed for plantlet production from embryos of Douglas-fir [Pseudotsuga menziesii (Mirb.) Franco]. The Medium for Conifer Morphogenesis, used at full strength, and supplemented with 10 M benzyladenine for 17 days produced an average of 6.8 shoots on more than 90% of the embryos. The percentage of shoot-forming embryos as well as the average number of shoots per embryo varied significantly among eight seedlots. For secondary multiplication, 89% of the adventitious shoots produced axillary buds on MCM with 5 M benzyladenine. However, 0.5 M BA was more suitable for the elongation of axillaries. Rooting ranged from 0–87% depending upon the treatment. The highest percentage was obtained with a 7-week incubation in peat:perlite containing 1/5-strength medium, 1% sucrose and 2.7 M naphthaleneacetic acid, followed by 5 weeks on peat:perlite with 1/5-strength major and minor salts, full iron and organics, 1% sucrose and 0.1% charcoal.  相似文献   

Stress exposure during the preimplantation period affects blastocyst lineages and offspring development     

Ján BURKU?  Martina KA?MAROVá  Janka KUBANDOVá  Natália KOKO?OVá  Kamila FABIANOVá  Du?an FABIAN  Juraj KOPPEL  ?tefan ?IKO? 《The Journal of reproduction and development》2015,61(4):325-331

We found retardation of preimplantation embryo growth after exposure to maternal restraint stress during the preimplantation period in our previous study. In the present study, we evaluated the impact of preimplantation maternal restraint stress on the distribution of inner cell mass (ICM) and trophectoderm (TE) cells in mouse blastocysts, and its possible effect on physiological development of offspring. We exposed spontaneously ovulating female mice to restraint stress for 30 min three times a day during the preimplantation period, and this treatment caused a significant increase in blood serum corticosterone concentration. Microscopic evaluation of embryos showed that restraint stress significantly decreased cell counts per blastocyst. Comparing the effect of restraint stress on the two blastocyst cell lineages, we found that the reduction in TE cells was more substantial than the reduction in ICM cells, which resulted in an increased ICM/TE ratio in blastocysts isolated from stressed dams compared with controls. Restraint stress reduced the number of implantation sites in uteri, significantly delayed eye opening in delivered mice, and altered their behavior in terms of two parameters (scratching on the base of an open field test apparatus, time spent in central zone) as well. Moreover, prenatally stressed offspring had significantly lower body weights and in 5-week old females delivered from stressed dams, fat deposits were significantly lower. Our results indicate that exposure to stress during very early pregnancy can have a negative impact on embryonic development with consequences reaching into postnatal life.  相似文献   

Alterations in oxidant/antioxidant balance,high-mobility group box 1 protein and acute phase response in cross-bred suckling piglets suffering from rotaviral enteritis     

Ujjwal Kumar De  Reena Mukherjee  Sukdeb Nandi  Bhimnere Hanumatnagouda Manjunatha Patel  Umesh Dimri  Chintu Ravishankar  Ashok Kumar Verma 《Tropical animal health and production》2014,46(7):1127-1133

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Serial analysis of serum cardiac troponin I changes and correlation with clinical findings in 46 dogs with mitral valve disease     

Zoe S. Polizopoulou  Christos K. Koutinas  Anastasia Dasopoulou  Michael Patsikas  Malcolm York  Ian Roman  Mitul Gandhi  Sonal Patel  Alexander F. Koutinas  Peter J. O'Brien 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2014,43(2):218-225

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Rheological properties of pasta dough during pasta extrusion: Effect of moisture and dough formulation     

Elena de la Peña  Frank A. Manthey  Bhavesh K. Patel  Osvaldo H. Campanella 《Journal of Cereal Science》2014

A study was conducted to investigate the effect of dough formulation and hydration level on the rheological properties of pasta dough during pasta extrusion. Semolina 100%, whole wheat 100%, and the following mixtures semolina-whole wheat (49:51), semolina-flaxseed flour (90:10), whole wheat-flaxseed flour (90:10), and semolina-whole wheat-flaxseed flour (39:51:10) were the formulations used for the experiments. Dough was hydrated at 30, 32, and 34% moisture content. Pasta was extruded with a capillary and a semi-commercial pasta extruder to determine the apparent viscosity of the dough during extrusion conditions and its relationship to the behavior of the dough during pasta processing. Results showed that non-traditional pasta dough behaved like a shear thinning fluid that can be described by the Power Law model. Increased hydration levels and/or presence of flaxseed flour on the dough formulation decreased the apparent viscosity of the dough, which correlated with extrusion pressure, mechanical energy, and specific mechanical energy that were required to extrude the dough in the pasta extruder. The strong correlations found between the apparent viscosity of the dough and the pasta extrusion parameters indicates the possibility of using a capillary rheometer to determine the appropriate hydration level of ingredient formulations before extruding with a pasta press.  相似文献   

Development and characterization of microsatellite markers,genetic diversity and population structure analysis in Sapota (Manilkara zapota (L.) P. Royen)     

Sathanandam  Pavithra N.  Patil  Prakash  Rekha  Ajitha  Muthuvel  Iyamperumal  Patel  Amrutlal R.  Boggala  Ramesh B.  Shirol  Adiveppa M.  Ravishankar  Kundapura V. 《Genetic Resources and Crop Evolution》2022,69(8):2787-2801

Genetic Resources and Crop Evolution - Manilkara zapota (L.) P. Royen, a widely adapted and popular tree meant for its appetizing fruits in tropics with no genomic resources like microsatellite...  相似文献   

Integrated use of organic and inorganic inputs in wheat-fodder maize cropping sequence to improve crop yields and soil properties     

Dharmsinh D. Rathod  Kishorbhai P. Patel  Khushvadan C. Patel 《Archives of Agronomy and Soil Science》2013,59(11):1439-1455

A 2-year field study was conducted to evaluate the effect of two organics, farmyard manure and vermicompost, each at three rates (0, 5, 10 t ha^?1 and 0, 1, 2 t ha^?1, respectively), along with two levels of mineral fertilizer (75% and 100% of recommended dose), on crops yields and soil properties under a wheat–fodder maize cropping sequence. Individual addition of organics at a higher level increased yields of wheat and subsequent maize. Soil microbial biomass carbon was enhanced as both a direct and residual effect with the addition of farmyard manure followed by vermicompost and fertilizer treatments, and also by combined addition of manure with either vermicompost or mineral fertilizer. Farmyard manure increased the availability of soil macro- and micronutrients, whereas vermicompost influenced only the availability of micronutrients at wheat harvest. A residual effect of farmyard manure and mineral fertilizers was found for available N. Meanwhile, the residual status of micronutrients in the soil was either maintained or significantly improved due to organic amendments (Mn and Zn with farmyard manure; Fe and Zn with vermicompost). Interaction of farmyard manure and vermicompost at a higher level benefited the next crop by increasing the yield of fodder maize and improving the availability of P and metals in soil.  相似文献   

Genetical relationships between reactions to bacterial leaf spot,yellow mosaic and Cercospora leaf spot diseases in mungbean (Vigna radiata)     

R. P. Thakur  P. N. Patel  J. P. Verma 《Euphytica》1977,26(3):765-774

Summary Studies on the inheritance pattern of bacterial leaf spot (BLS), yellow mosaic (YM) and Cercospora leaf spot (CLS) reactions in crosses of BLS and YM resistant/tolerant but CLS susceptible × CLS resistant but BLS and YM susceptible parents indicated that resistances to BLS and CLS were governed by single dominant genes, whereas YM tolerance was a monogenic recessive character. The studies also indicated that these three genes were inherited independently. The simple inheritance pattern and independent assortment of the genes governing resistance/tolerance to these diseases suggest that the usual breeding methods will be adequate to develop multi-disease resistant mungbean cultivars.Paper XII in the series Studies on resistance in crops to bacterial diseases in India.  相似文献   

Pressure-induced unfolding and aggregation of the proteins in whey protein concentrate solutions     

Patel HA  Singh H  Havea P  Considine T  Creamer LK 《Journal of agricultural and food chemistry》2005,53(24):9590-9601

Whey protein concentrate solutions (12% w/v, pH 6.65 +/- 0.05) were pressure treated at 800 MPa for 20-120 min and then examined using size exclusion chromatography (SEC), small deformation rheology, transmission electron microscopy, and various types of one-dimensional (1D) and two-dimensional (2D) polyacrylamide gel electrophoresis (PAGE). The pressure-treated samples showed a time-dependent loss of native whey proteins by SEC and 1D PAGE and a corresponding increase in non-native proteins and protein aggregates of different sizes. These aggregates altered the viscosity and opacity of the samples and were shown to be cross-linked by intermolecular disulfide bonds and by noncovalent interactions using 1D PAGE [alkaline (or native), sodium dodecyl sulfate (SDS), and SDS of reduced samples (SDS(R))] and 2D PAGE (native:SDS and SDS:SDS(R)). The sensitivity of the major whey proteins to pressure was in the order beta-lactoglobulin B (beta-LG B) > beta-LG A > bovine serum albumin (BSA) > alpha-lactalbumin (alpha-LA), and the large internal hydrophobic cavity of beta-LG may have been partially responsible for its sensitivity to high-pressure treatments. It seemed likely that, at 800 MPa, the formation of a beta-LG disulfide-bonded network preceded the formation of disulfide bonds between alpha-LA or BSA and beta-LG to form multiprotein aggregates, possibly because the disulfide bonds of alpha-LA and BSA are less exposed than those of beta-LG either during or after pressure treatment. It may be possible that intermolecular disulfide bond formation occurred at high pressure and that hydrophobic association became important after the high-pressure treatment.  相似文献