Evaluation of a fusion gene-based DNA prime-protein boost vaccination strategy against Newcastle disease virus     

Khulape  Sagar A.  Maity  Hemanta Kumar  Pathak  Dinesh Chandra  Ramamurthy  Narayan  Ramakrishnan  Saravanan  Chellappa  Madhan Mohan  Dey  Sohini 《Tropical animal health and production》2019,51(8):2529-2538

Tropical Animal Health and Production - The low potency of genetic immunization has to date impeded development of commercial vaccines against major infectious diseases. The aim of this study was...  相似文献   

The impact of bisphenol S on bovine granulosa and theca cells          下载免费PDF全文

KA Campen  M Lavallee  CMH Combelles 《Reproduction in domestic animals》2018,53(2):450-457

Bisphenol S (BPS) is an endocrine‐disrupting chemical with multiple potential mechanisms of action, including as an oestrogen receptor agonist. BPS is increasingly used in plastics and thermal receipts as a substitute for bisphenol A, which has been phased out due to concerns about human health implications. The ability of BPS to alter female reproductive function in mammals has not been widely studied, despite the importance of normal hormone signalling for female reproduction. The aim of this study was to investigate how BPS (in a wide range of doses, including very low doses) affects granulosa cell and theca cell steroid hormone production and cell viability in the bovine. Granulosa cell oestradiol production was stimulated when cells were exposed to 100 μM BPS under basal conditions, but there was no effect of BPS when cells were stimulated with follicle‐stimulating hormone (FSH). Additionally, there was no effect of BPS on granulosa cell progesterone production or cell viability under basal or FSH‐stimulated conditions. BPS did not affect theca cell androstenedione or progesterone production, or theca cell viability under basal or luteinizing hormone‐stimulated conditions. This study suggests for the first time that BPS may alter oestradiol production by bovine granulosa cells, albeit at a concentration that is unlikely to be physiologically relevant. Further studies are needed to determine the effects of BPS on the bovine oocyte and on other functions of follicular cells.  相似文献   

Chilli pepper genotypes resistant to Cercospora leaf spot and powdery mildew     

A. Deshpande  R. D. Rawal  D. P. Singh  C. S. Pathak 《国际虫害防治杂志》2013,59(4):470-471

  相似文献   

南疆灌区冬小麦/夏玉米改良模式增产潜力与机理分析     

章建新  赵明  图尔贡  卡地尔亚 《干旱地区农业研究》2013,31(6):15-21

田间试验系统地比较了新冬20/沈玉2002不同套种模式（“522模式”与“952模式”）及“521模式”下保麦10号/蠡玉35、保麦10号/登海3672、新冬20/蠡玉35的产量、叶面积指数、光合势及干物质积累等性状的差异。结果表明,“522模式”新冬20/沈玉2002周年产量（17 838.2 kg·hm^-2）较“952模式”(16 550.9 kg·hm^-2)增产7.8%,其中,冬小麦增产18.4%。“521模式”保麦10号/蠡玉35、保麦10号/登海3672、新冬20/蠡玉35周年产量为19 905.0～20 751.0 kg·hm^-2,其中,冬小麦产量为9 740～10 275 kg·hm^-2、夏玉米产量为9 630～10 211.0 kg·hm^-2;冬小麦孕穗期和开花期叶面积指数分别为6.0、5.0左右,返青至成熟的总光合势290.2～324.3×10⁴·m²·d·hm^-2;夏玉米生育期111 d,大喇叭口期至吐丝后20 d的叶面积指数均保持在4左右,生育期间总光合势233.1～257.5×10⁴·m²·d·hm^-2,冬小麦和玉米总光合势为523.3～581.8×10⁴·m²·d·hm^-2;总干物质量38 569.1～40 487.0 kg·hm^-2;冬小麦产量的边际效应为85.7%～112.9%。“521模式”比“952模式”大幅度增产,是小麦行数和边行数及边际产量增大的结果,并将夏玉米播期提至麦收前15 d左右,延长玉米生育期（达110 d左右）,优化株行配置使玉米增产显著,实现全年超高产。“521模式”同时充分利用了冬小麦边际补偿效应和玉米时间补偿效应,较“952模式”易获得周年超高产。并对超高产栽培技术进行了讨论。  相似文献   

Preliminary Studies on Cryopreservation of Common Tench (Tinca tinca) Embryos (Work in Progress)     

KA El-Battawy  O Linhart 《Reproduction in domestic animals》2009,44(4):718-723

Vitrification could provide a promising tool for the cryopreservation of fish embryos. However, to achieve cryopreservation using vitrification, chilling sensitivity and cryoprotectants toxicity were determined using tench embryos at four developmental stages (11, 17, 23 and 29 h). Embryos treated with alcalase (2 ml/998 ml, 2 min at 22°C) were exposed to chilling with/without warming. Other embryos were exposed to methanol and glycerol at the concentration of 10% and 20% for periods of 20 min. At last, embryos were incubated at special incubator cages where hatching rates were counted. Regarding chilling sensitivity and exposure to chilling followed by warming, the hatching rates of embryos decreased significantly (p < 0.001) after exposure to 0°C at all developmental stages except the 29-h stage compared with the controls. The embryo stage most sensitive to chilling was 11-h stage. The 29-h stage exhibited the least sensitivity to low temperature while 17-h and 23-h stages were intermediate in their sensitivity to chilling. The toxicity of methanol increased significantly (p < 0.001) with developmental stage for 11, 17 and 23-h stages. The highest hatching rates of tench embryos were obtained with 29-h embryos using various concentrations of methanol. The hatching rates of tench embryos exposed to glycerol concentrations were approximately similar to those embryos exposed to methanol concentrations except for 11-h embryos that showed no hatching. Unfortunately, we could not obtain living embryos in any of the conditions examined after vitrification. In conclusion, it was quite difficult to vitrify the tench embryos during this study using various vitrifying solutions and the method reported by Chen & Tian (2005) and further studies are needed to achieve successful cryopreservation.  相似文献   

Reproductive performance of crossbred dairy cows reared under traditional low input production system in the eastern Himalayas     

A. Kumaresan  P. P. Prabhakaran  K. M. Bujarbaruah  K. A. Pathak  Bijoy Chhetri  S. K. Ahmed 《Tropical animal health and production》2009,41(1):71-78

Reproductive performance of crossbred cows reared under traditional low input production system was assessed. A total number of 160 farmers were surveyed and traditional fodders were evaluated for proximate composition. Total protein, albumin and cholesterol concentrations in blood serum were analyzed. The age at first service, age at first calving, calving interval, calving to first service and calving to conception interval were calculated from records of 261 crossbred dairy cows. The crude protein, crude fiber, nitrogen free extract, ether extract and total ash content varied from 7.2 to 13.9, 18.2 to 34.4, 39.1 to 59.2, 2.1 to 4.1 and 7.2 to 17.9%, respectively. The total protein and albumin concentrations in blood serum were 7.6 ± 0.3 and 4.3 ± 0.3 g/dl, respectively. The cholesterol concentration was 221.1 ± 8.2mg/dl. The mean age at first service and age at first calving was 28.6 ± 1.0 and 40.7 ± 1.1months, respectively. The mean values for calving to first service and calving to conception intervals were 182 ± 14.5 and 224 ± 9.0days, respectively. The conception rate was significantly high among the cows that showed typical fern pattern of cervical mucus (56.6%). The conception rate based on 1^st insemination was 44.4%. The incidence of repeat breeders and anestrus among the crossbred cows were 12.8 and 14.1%, respectively.  相似文献   

Inheritance of resistance to whitebacked planthopper,Sogatella furcifera (Horvath) in some rice cultivars     

J. P. Singh  M. P. Pandey  P. K. Pathak 《Euphytica》1990,46(2):95-100

Summary The genetics of resistance to whitebacked planthopper, Sogatella furcifera (Horvath) in ten resistant cultivars was studied. The reactions of the F₁, F₂ and F₃ populations of resistant varieties with Taichung Native 1, a suspectible check, showed that WBPH resistance is monogenic in nature and governed by dominant gene(s) in Ptb 19 and IET 6288 and recessive gene in eight cultivars viz. ARC 5838, ARC 6579, ARC 6624, ARC 10464, ACR 11321, ARC 11320, Balamawee and IR 2415-90-4-3. Allelic relationship of resistance gene(s) in the test cultivars revealed recessive gene in IR 2415-90-4-3, ARC 5838 and ARC 11324 to be allelic but it was non allelic to the resistance gene in ARC 6624. Cultivars ARC 6579, ARC 11321 and Balamawee have identical gene among themselves but their relationship with IR 2415-90-4-3, ARC 5838, ARC 11324 and ARC 6624 is unknown. The recessive gene in ARC 10464 is non-identical to all other cultivars having the recessive gene except ARC 6624 with which its relationship needs further investigation.  相似文献   

Advances in therapeutic and managemental approaches of bovine mastitis: a comprehensive review     

Khan Sharun  Kuldeep Dhama  Ruchi Tiwari  Mudasir Bashir Gugjoo  Mohd. Iqbal Yatoo  Shailesh Kumar Patel  Mamta Pathak  Kumaragurubaran Karthik  Sandip Kumar Khurana  Rahul Singh  Bhavani Puvvala  Amarpal  Rajendra Singh  Karam Pal Singh  Wanpen Chaicumpa 《The Veterinary quarterly》2021,41(1):107

Mastitis (intramammary inflammation) caused by infectious pathogens is still considered a devastating condition of dairy animals affecting animal welfare as well as economically incurring huge losses to the dairy industry by means of decreased production performance and increased culling rates. Bovine mastitis is the inflammation of the mammary glands/udder of bovines, caused by bacterial pathogens, in most cases. Routine diagnosis is based on clinical and subclinical forms of the disease. This underlines the significance of early and rapid identification/detection of etiological agents at the farm level, for which several diagnostic techniques have been developed. Therapeutic regimens such as antibiotics, immunotherapy, bacteriocins, bacteriophages, antimicrobial peptides, probiotics, stem cell therapy, native secretory factors, nutritional, dry cow and lactation therapy, genetic selection, herbs, and nanoparticle technology-based therapy have been evaluated for their efficacy in the treatment of mastitis. Even though several strategies have been developed over the years for the purpose of managing both clinical and subclinical forms of mastitis, all of them lacked the efficacy to eliminate the associated etiological agent when used as a monotherapy. Further, research has to be directed towards the development of new therapeutic agents/techniques that can both replace conventional techniques and also solve the problem of emerging antibiotic resistance. The objective of the present review is to describe the etiological agents, pathogenesis, and diagnosis in brief along with an extensive discussion on the advances in the treatment and management of mastitis, which would help safeguard the health of dairy animals.  相似文献   

InfoCrop: A dynamic simulation model for the assessment of crop yields,losses due to pests,and environmental impact of agro-ecosystems in tropical environments. I. Model description     

P.K. Aggarwal  N. KalraS. Chander  H. Pathak 《Agricultural Systems》2006

The problems of agriculture in many tropical countries are gradually becoming more intense due to increasing food demand led by population growth, stagnation in farm productivity, mounting yield losses due to multiple pests, increasing vulnerability to global environmental changes and the need to reduce emission of greenhouse gases. Tools and techniques are needed to assist in developing strategies that can lead to higher food production, prevent crop production losses, and ensure minimal greenhouse gas emissions while maintaining soil fertility. Several dynamic models have been developed in recent past but most of these are generally strong either in soils and crops, or in greenhouse gases (GHG) emissions. Pest induced yield losses, a critical issue in the tropics, is not addressed in most models. InfoCrop, a generic dynamic crop model, has been developed to meet these specific requirements. It provides integrated assessment of the effect of weather, variety, pests, soil and management practices on crop growth and yield, as well as on soil nitrogen and organic carbon dynamics in aerobic as well as anaerobic conditions, and greenhouse gas emissions. The model considers the key processes related to crop growth, effects of water deficit, flooding, nitrogen management, temperature and frost stresses, crop–pest interactions, soil water and nitrogen balance and (soil) organic carbon dynamics. Its general structure relating to basic crop growth and yield is largely based on several earlier models, especially SUCROS series, and is written in Fortran Simulation Environment (FSE) programming language. The model has been validated for dry matter and grain yields of several annual crops, losses due to multiple diseases and pests, and emissions of carbon dioxide, methane and nitrous oxide in a variety of agro-environments. To increase the applications of model in research and development, an extremely simple menu driven version of InfoCrop has also been developed. The users of this version do not need any background in programming.  相似文献   

Trichostrongylus axei infection as a cause of deaths and loss of weight in sheep     

KA ABBOTT  IJ MCFARLAND 《Australian veterinary journal》1991,68(11):368-369

  相似文献