Rearing of African catfish (Clarias gariepinus) and vundu catfish (Heterobranchus longifilis) in traditional fish ponds (whedos): Effect of stocking density on growth, production and body composition     

Imorou Toko  Emile D. Fiogbe  Patrick Kestemont 《Aquaculture (Amsterdam, Netherlands)》2007,262(1):65-72

African catfish (Clarias gariepinus) (initial body weight: 34.8 ± 4.8 g) and vundu catfish (Heterobranchus longifilis) (initial body weight: 39.1 ± 8.2 g) fingerlings were stocked at densities of 4, 6 or 8 fish m^− 3 in traditional fish ponds (whedos) constructed in the floodplain of the Oueme River (South Benin, West Africa), for 70 days from March to June 2005. Fish were fed twice a day with 34% crude protein feed formulated with locally available ingredients. The effects of stocking density were evaluated in growth responses, gross production and body composition. Water quality variables were similar (p > 0.05) in all compartments. Temperature and pH were at the optimum level for fish. Dissolved oxygen ranged from 0.9 to 1.2 mg l^− 1 during the experiment and secchi disc transparency was low (< 14 cm). In both species, growth responses increased with the increasing density, significantly in African catfish stocked at density of 8 fish m^− 3 compared to the other densities (4 and 6 fish m^− 3) but not significantly in vundu catfish. Production data ranged from 3.1 ± 0.5 to 22.8 ± 4.5 t ha^− 1 year^− 1 in African catfish and from 6.1 ± 1.2 to 15.1 ± 3.1 t ha^− 1 year^− 1 in vundu catfish. Production increased with increasing stocking densities but only significantly (p < 0.05) between the density of 8 fish m^− 3 and the other densities. In both species, carcass fat increased with increasing density (p < 0.05) while carcass protein and moisture decreased (p > 0.05). These results are important because they indicate that, as far as growth rate and production are concerned, African catfish is more profitable than vundu catfish for culture at high density in whedo.  相似文献   

Determinism of the induction of the reproductive cycle in female Eurasian perch, Perca fluviatilis: Identification of environmental cues and permissive factors     

Neil Wang  Emilie Henrotte  Patrick Kestemont 《Aquaculture (Amsterdam, Netherlands)》2006,261(2):706-714

In Eurasian perch, Perca fluviatilis, gametogenesis is induced by decrease of both temperature and photoperiod. However, a multiplicity of other factors can display non-permissive or modulating effects on the induction of the reproductive cycle. Consequently, a 9-week study was carried out to determine the environmental cues and permissive factors controlling the induction of the reproductive cycle in this species. A two-level fractional factorial design 2^8-4 was used in order to identify the influent factors from 8 environmental and nutritional ones and their 28 interactions. Tested factors were: (1) amplitude of temperature decrease, (2) time, (3) kinetics and (4) amplitude of photoperiod decrease, (5) initial nutritional state, (6) handling, (7) feeding rate and (8) light spectrum. Increase of gonadosomatic index (GSIi), proportion of female in exogenous vitellogenesis (EVP), plasma 17 β-estradiol (E₂) and cortisol levels, fat consumption and food intake were evaluated.This study showed that the amplitude of the decrease of temperature and the time of photoperiod decrease are the two main environmental cues controlling the induction of the reproductive cycle in Eurasian perch. GSIi was the highest (3.8%) when females were exposed to both low amplitude of temperature decrease and precocious decrease of photophase. It was the lowest (1.1%) when high amplitude of temperature decrease and late decrease of photophase were applied. Handling in association with temperature modulated the broodstock response. 100% of the unhandled fish held at 14 °C were at the exogenous vitellogenesis stage with mean E₂ and basal cortisol levels of 1.6 ng mL^− 1 and 9 ng mL^− 1 respectively. Handled fish and fish held at 6 °C exhibited lower vitellogenesis (40-73%) associated with lower E₂ (0.6-1.1 ng mL^− 1) and higher basal cortisol levels (37-89 ng mL^− 1). No other factor nor interaction displayed a cueing or non permissive effect on the induction of the reproductive cycle in female Eurasian perch. A first schematic model is proposed to explain the factorial determinism of the induction of the reproductive cycle.  相似文献   

Hormonal regulation of metabolism in hepatocytes of the ureogenic teleostopsanus beta     

Dr Thomas P. Mommsen  Eva Danulat  Patrick J. Walsh 《Fish physiology and biochemistry》1991,9(3):247-252

Short-term exposure of isolated toadfish hepatocytes to high concentrations (100 nM) of glucagon, glucagon-like peptide (GLP) or epinephrine significantly increases the rate of lactate gluconeogenesis (1.3-fold) and glycogenolysis (5- to 7-fold). Half-maximal responsiveness to GLP is reached at about 2 nM for gluconeogenesis and 6 nM for glycogenolysis, while the value for glycogenolysis activated by catfish glucagon is 28 nM. Cells do not to respond to 5 nM epinephrine. Norepinephrine, urotensin II and leucine-enkephalin, each applied at 100 nM, increase the rate of glycogenolysis by 1.3 to 1.5-fold. All other hormones tested (vasotocin, isotocin, VIP, methionine-enkephalin, ovine prolactin, -endorphin, APY, salmon insulin) failed to affect metabolic flux through glycogenolysis or gluconeogenesis. None of the hormones altered the rate of urea synthesis or the rate of lactate oxidation by hepatocytes. Although toadfish hepatocytes are responsive to hormonal stimuli, they do not appear to be a useful model to study evolutionary trends in short-term hormonal regulation of urea synthesis. However, the obvious differences in mechanisms of control of urea synthesis in this species compared with ureogenic amphibians and mammals open an intriguing avenue for research.  相似文献   

Using ecological trajectories to track long-term taxonomic and functional changes in benthic shallow soft-bottom communities (Bay of Saint-Brieuc,English Channel)     

Anthony Sturbois  Gaëtan Cormy  Alexandre Le Moal  Gauthier Schaal  Caroline Broudin  Eric Thiébaut  Alain Ponsero  Patrick Le Mao  Auriane Jones  Pascal Riera  Olivier Gauthier  Nicolas Desroy 《水产资源保护:海洋与淡水生态系统》2021,31(11):3013-3033

1. Taxonomic and functional trajectories of benthic assemblages were studied in shallow soft-bottom sediments in the bay of Saint-Brieuc (Western English Channel). Changes were assessed at different spatial and temporal scales using a macrobenthic dataset based on 38 stations sampled in 1987 and 2019, coupled with data from one station sampled annually between 2005 and 2019 as part of the European Water Framework Directive monitoring programme.
2. Taxonomic trajectories indicated 1) changes in the structure and distribution of benthic assemblages, 2) an homogenization of the assemblages, and 3) significant functional shifts.
3. Fishing activities and nutrient enrichment are probably strong drivers of the observed changes, as suggested by the higher mortality rate of fragile, flexible, tubiculous and burrowing species, and the increase in the abundance of opportunistic species in the assemblages. Certain populations of macroinvertebrates seem jointly controlled by climate change and by the aforementioned local factors of disturbance.
4. The Community Trajectory Analysis framework appears as a new and interesting method to track ecological changes in marine ecosystems by measuring change with respect to a baseline state, to help define ecological recovery (station returning to the initial ecological state) and departure (station presenting increased changes over time), and to analyse trajectory similarity.
5. According to the degradation of habitat over time, we identify the need for the implementation of knowledge-based conservation strategies, especially within Natura 2000 sites.

  相似文献   

Ditch communities: a major contributor to floodplain biodiversity     

Patrick D. Armitage  Krzysztof Szoszkiewicz  John H. Blackburn  Ian Nesbitt 《水产资源保护:海洋与淡水生态系统》2003,13(2):165-185

• 1. Chalk stream floodplains are extensively managed and the high degree of connectivity associated with water meadows has been severely reduced. The main contributor to aquatic biodiversity is perceived to be the river itself. Ditches in these areas have been largely ignored as a contributor to total diversity.
• 2. Separate surveys of instream, bankside and ditch macroinvertebrates in a small area of floodplain provided a unique opportunity to assess the relative contribution of each locality to total aquatic diversity. The fauna and flora of a ditch on the lower River Frome floodplain was examined and compared with studies of adjacent bankside and instream habitat. A total of 145 macroinvertebrate taxa and 61 plant species were recorded from the ditch. Faunal abundance and richness were positively related to the diversity of aquatic plants. The ditch showed a clear linear trend in community type that appeared to be partly related to the acidic seepage from tertiary deposits at the source and percentage cover of vegetation.
• 3. There were large differences in the numbers of taxa collected in ditch, bankside, instream mesohabitat and RIVPACS surveys. Of the total 202 taxa recorded in the four surveys, the ditch contributed 59 unique taxa with 25 from the bankside and two and three from the mesohabitat and RIVPACS surveys, respectively.
• 4. The large contribution of the Rushton Ditch to aquatic biodiversity on the floodplain is due to three main factors: the chemically different and slow‐flowing environment, the connection to the main river, and a management regime which has halted hydroseral succession at a point where vegetation structure is for the most part open and able to support a wide range of ecological niches. The maintenance of these conditions requires sensitive management and further knowledge of the needs and interactions of faunal communities with vegetation.

Copyright © 2002 John Wiley & Sons, Ltd.  相似文献   

Effects of weaning age and diets on ontogeny of digestive activities and structures of pikeperch (<Emphasis Type="Italic">Sander lucioperca</Emphasis>) larvae     

Neila Hamza  Mohamed Mhetli  Patrick Kestemont 《Fish physiology and biochemistry》2007,33(2):121-133

Growth and ontogeny of digestive function were studied in pikeperch (Sander lucioperca) larvae weaned on artificial food at different ages. Three weaning treatments initiated respectively on day 9 (W9), day 15 (W15) or day 21 (W21) post-hatching (p.h.) were compared with a control group, fed Artemia nauplii from first feeding until the end of the rearing trial on day 36 p.h. The digestive enzyme activities and the ontogeny of digestive structures were investigated using enzymatic assays and histological methods. Growth of pikeperch larvae was significantly affected by precocious weaning. Pancreatic (trypsin and amylase) and intestinal (leucine-alanine peptidase, leucine aminopeptidase N and alkaline phosphatase) enzyme activities were detected from hatching onwards, increased at the moment of first feeding and then decreased. Pepsin secretion occurred at day 29 p. h. only, concurrently with the stomach development and differentiation of gastric glands. In the early weaning group (W9) the maturation process of intestinal enterocytes seems to be impaired and/or delayed and several signs of malnutrition were recorded. Except for alkaline phosphatase activity, no differences in enzyme activities and development of digestive structures were observed among the control, W21, and W15 groups. Moreover, at the end of the experiment, no differences in proteolytic activities were observed among larvae from the different treatments, indicating that, in surviving individuals, the digestive structures were properly developed and the larvae had acquired an adult mode of digestion. Based on the artificial diet used, our results suggested that pikeperch larvae can be weaned from day 15 p.h. without significant adverse effect on digestive capacities (except for alkaline phosphatase) or development of digestive tract, while earlier weaning impaired the onset of the maturation processes of the digestive system, both in terms of morphological structures and enzymatic activities.  相似文献   

Tyrosine hydroxylase activity and dopamine turnover of rainbow trout (Oncorhynchus mykiss) brain: the special status of the hypothalamus   总被引：1，自引：0，他引：1  

Boris Linard  Sanae Bennani  Patrick Jego  Christian Saligaut 《Fish physiology and biochemistry》1996,15(1):41-48

The dynamics of catecholamine (CA)-synthesis enzymes have been poorly studied in fish. Tyrosine hydroxylase (TH), the rate-limiting enzyme of CA synthesis has been only studied inin vitro conditions. In the present report thein vivo CA synthesis and the CA metabolism were studied in different regions of the forebrain of the rainbow trout. Levels of norepinephrine (NE), dopamine (DA), 3,4-dihydroxyphenylacetic acid (DOPAC) and the rate of accumulation of 3,4-dihydroxyphenylalanine (DOPA) were determined by HPLC following a treatment with hydroxybenzylhydrazine (NSD), a potential inhibitor of DOPA decarboxylase. Kinetics of the accumulation of DOPA and of the decline of DOPAC were in agreement with those found in rat, evidencing that the accumulation of DOPA following NSD can be used in trout to quantify thein vivo enzymatic activity of tyrosine hydroxylase. Experiments using treatment with NSD or with methyl-p-tyrosine reached a same conclusion: the DA neuronal activity in trout is much higher than NE neuronal activity. However, the hypothalamus had high DA levelsvs. lowin vitro andin vivo TH activities and exhibited a low CA turnover.  相似文献   

How well does a botanical garden collection of a rare palm capture the genetic variation in a wild population?   总被引：1，自引：0，他引：1  

Sandra Namoff  Javier Francisco-Ortega  Larry R. Noblick  M. Patrick Griffith 《Biological conservation》2010,143(5):1110-1117

Conservation is increasingly central to the botanic garden mission. Living plant collections are important components of conservation. Critical evaluation of living conservation collections with population genetic analysis can directly inform ex situ conservation strategy. Here, we quantify the degree of genetic variation captured through a population-based collection protocol, and explore optimal sampling for ex situ conservation. An extensive living collection derived from one population of Leucothrinax morrisii (Arecaceae) provided a model system. We compared 58 specimens from the ex situ collection with 100 individuals from throughout the parent population via 6 ISSR loci. Random bootstrapped resamples of the data were made to model differently structured ex situ collections. Mean diversity (He) differed little between the collection (0.204) and the population (0.216), and genetic distance (D) was very close (0.036). Very few private alleles were found between the collection and the population. Allelic capture, as measured by percent of private alleles, was greater than 94%. Resampled collections of different sizes captured from 48% to 94% of alleles. Pairwise comparison of bootstrapped resamples suggests that increasing the representation of half-sibling groups does not significantly increase allele capture. Increase in allele capture with increasing sample size is greatest at low resample sizes, and showed diminishing returns as resample size increased. No appreciable increase in allele capture was gained through maintaining different half-sibling groups. These data inform sampling for ex situ conservation purposes, and recommend sample sizes of at least 15 individuals, with the upper limit based on resources.  相似文献   

A new approach to trenching experiments for measuring root-rhizosphere respiration in a lowland tropical forest     

E.J. Sayer  E.V.J. Tanner 《Soil biology & biochemistry》2010,42(2):347-863

Soil respiration in tropical forests is a major source of atmospheric CO₂. The ability to partition soil respiration into its individual components is becoming increasingly important to predict the effects of disturbance on CO₂ efflux from the soil as the responses of heterotrophic and autotrophic respiration to change are likely to differ. However, current field methods to partition respiration suffer from various methodological artefacts; root-rhizosphere respiration is particularly difficult to estimate. We used trenched subplots to estimate root-rhizosphere respiration in large-scale litter addition (L+), litter removal (L−) and control (CT) plots in a lowland tropical semi-evergreen forest in Panama. We took a new approach to trenching by making measurements immediately before-and-after trenching and comparing them to biweekly measurements made over one year. Root-rhizosphere respiration was estimated to be 38%, 17% and 27% in the CT, L+, and L− plots, respectively, from the measurements taken immediately before and one day after trenching in May-June 2007. Biweekly measurements over the following year provided no estimates of root-rhizosphere respiration for the first seven months due to decomposition of decaying roots. We were also unable to estimate root-rhizosphere respiration during the dry season due to differences in soil water content between trenched and untrenched soil. However, biweekly measurements taken during the early rainy season one year after trenching (May-June 2008) provided estimates of root-rhizosphere respiration of 39%, 24% and 36% in the CT, L+, and L− plots, respectively, which are very similar to those obtained during the first day after trenching. We suggest that measurements taken immediately before and one day after root excision are a viable method for a rapid estimation of root-rhizosphere respiration without the methodological artefacts usually associated with trenching experiments.  相似文献   

Comparison of Docosahexaenoic Acid (22:6n-3) Levels in Various Artemis Strains during Enrichment and Subsequent Starvation     

Kyungmin  Han  Inge  Geurden  Patrick  Sorgeloos 《Journal of the World Aquaculture Society》2000,31(3):469-475

Abstract.— The aim of this study was to compare the levels of docosahexaenoic acid (DHA, 22:6n-3) in three different bisexual and one parthenogenetic strains of Artemia after enrichment. Freshly-hatched nauplii from A. franciscana (Great Salt Lake, USA), A. sinica (Yimeng, P. R. China), A. persimilis (Argentina), and A. parthenogenetica (Tanggu, P. R. China) were enriched with a purified lipid emulsion containing 95% DHA ethyl esters (% total fatty acids) and subsequently starved. All strains had very low initial DHA levels (< 0.3 mg/g dry weight). Initial eicosapentaenoic acid, 20:5n-3 (EPA) levels were high in A. parthenogenetica (18.2 mg/g dry weight) as compared to those in the other strains (4.6-8.5 mg/g dry weight). After 24-h enrichment, A. sinica contained the highest DHA level (37.0 mg/g dry weight) as well as the highest DHA/ EPA ratio (3.7). The lowest DHA enrichment levels were found in A. franciscana and A. parthenogenetica (26.3 and 22.7 mg/g dry weight, respectively). During the subsequent 24-h starvation period, the contents of DHA decreased rapidly in all strains, whereas EPA levels remained relatively stable. This indicates the high catabolism of DHA for energy production, the relative conservation of EPA, and possibly a partial bioconversion of DHA to EPA during the starvation period in each species.  相似文献