Laser-based spectroscopic methods to evaluate the humification degree of soil organic matter in whole soils: a review     

Giorgio S. Senesi  Ladislau Martin-Neto  Paulino Ribeiro Villas-Boas  Gustavo Nicolodelli  Débora M. B. P. Milori 《Journal of Soils and Sediments》2018,18(4):1292-1302

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Net requirements of calcium, magnesium, sodium, phosphorus, and potassium for growth of Nellore × Red Angus bulls, steers, and heifers   总被引：1，自引：0，他引：1  

Mario Luiz Chizzotti  Sebastio de Campos Valadares Filho  Luis Orlindo Tedeschi  Pedro Veiga Rodrigues Paulino  Mrio Fonseca Paulino  Rilene Ferreira Diniz Valadares  Paloma Amaral  Pedro Del Bianco Benedeti  Tainnah Ikegami Rodrigues  Mozart Alves Fonseca 《Livestock Science》2009,124(1-3):242-247

A comparative slaughter trial was conducted to assess the net requirements for gain of Ca, P, Na, K and Mg of bulls, steers and heifers of Nellore and Red Angus crossbreds. Twenty seven F1 Nellore and Red Angus crossbred calves (9 steers, 9 bulls, and 9 heifers), averaging 274 kg BW, were used. At the beginning of the trial, three animals from each gender were slaughtered to determine the initial body composition. The remaining 18 animals (3 animals of each gender) were randomly assigned to two treatments: fed 0.75 or 1.5% of BW of concentrate. The diets were based on corn silage and were isonitrogenous (2% N, DM). After three growing periods of 28 d, all animals were slaughtered. The cleaned gastrointestinal tract, organs, carcass, head, hide, tail, feet, and tissues were weighed to determine the empty BW (EBW). These parts were ground separately and subsampled for chemical analyses. The log of the contents of each mineral in the empty body was regressed on the log of the EBW to estimate the net requirement for each mineral per kg of empty body gain (EBG). There were no differences (P > 0.05) in the net requirements for growth of all macrominerals among genders. The equations of the pooled data of the net requirements for growth (g/kg EBG) were: 332.6 × EBW ^− 0.6367 for Ca, 112.1 × EBW ^− 0.5615 for P, 10.85 × EBW ^− 0.3992 for Na, 4.01 × EBW ^− 0.153 for K, and 3.589 × EBW ^− 0.462 for Mg. Our findings indicated that retained Ca and retained P were poorly related to the retained protein.  相似文献   

Parameterization of ruminal fibre degradation in low-quality tropical forage using Michaelis–Menten kinetics     

Edenio Detmann  Mrio F. Paulino  Hilrio C. Mantovani  Sebastio de C. Valadares Filho  Cludia B. Sampaio  Marjorrie A. de Souza  Ísis Lazzarini  Kelly S.C. Detmann 《Livestock Science》2009,126(1-3):136-146

This work aimed to parameterize the ruminal degradation of neutral detergent fibre (NDF) from low-quality tropical forage using Michaelis–Menten kinetics. The intake, rumen outflow (L), fractional degradation rate (kd), discrete lag (LAG) and effective degradability (ED) of NDF, and the microbial flow of nitrogenous compounds into the small intestine (Nmic) were assessed in two 5 ×  5 Latin square experiments by using five Holstein × Zebu heifers cannulated in the rumen. The experiments were carried out sequentially and the treatments were formed by increasing the level of supplementation with nitrogenous compounds. A low-quality signal grass (Brachiaria decumbens) hay was used as roughage. The nitrogen supplement was a mixture of urea, ammonium sulfate and albumin, at the ratios of 4.5:0.5:1.0, respectively. The crude protein contents in the diets ranged from 51.9 to 136.3 g/kg of dry matter. The rumen ammonia nitrogen (RAN) concentration was used as an independent variable. The NDF intake, L and Nmic showed a quadratic pattern (P < 0.05) as a function of RAN concentration, and the critical points (maximum responses) were observed with 15.17, 16.28, and 14.52 mg of RAN/dL of rumen fluid, respectively. On the other hand, ED and LAG presented a linear-response-plateau (P < 0.05) according to the RAN concentration, with break points close to 8 mg/dL for ED (maximum estimate) and LAG (minimum estimate). The RAN concentrations to optimize NDF degradation and intake were defined as 8 and 15 mg/dL, respectively. This difference between estimates appears to be due to a better adequacy of the metabolizable protein:metabolizable energy ratio in the animal metabolism, which increases the animal intake even after the rumen NDF degradation has been optimized. This observation was supported by Nmic pattern. An adapted Michaelis–Menten model was applied to the data, where RAN was the independent variable and kd the dependent variable. The relationship between these variables was found to be significant by using the Hanes–Woolf plot (P < 0.01). Based on this model, the rate of NDF degradation as a function of RAN concentration indicates that fibre degradation in the rumen could be considered a second order process. In this context, the RAN concentration of 8 mg/dL was assumed as the limit where zero order (below limit) and first order (above limit) reactions become predominant for NDF degradation in the rumen.  相似文献   

Performance and meat quality traits of beef heifers fed with two levels of concentrate and ruminally undegradable protein     

Duarte Mde S  Paulino PV  Valadares Filho Sde C  Paulino MF  Detmann E  Zervoudakis JT  Monnerat JP  Viana Gda S  Silva LH  Serão NV 《Tropical animal health and production》2011,43(4):877-886

The effects of two levels of concentrate and ruminally undegradable protein (RUP) on performance, intake, digestibility, carcass characteristics, meat quality traits, and commercial cuts yield were assessed. Twenty crossbred heifers (240 kg average body weight) were used. At the beginning of the trial, four animals were slaughtered as reference group and the 16 remaining animals were randomly assigned to four treatments, in a 2 × 2 factorial design: two levels of concentrate (40% and 80%, dry matter (DM) basis) and two levels of RUP (48.79% and 27.19% of CP). At the end of the trial, all the animals were slaughtered. There was no interaction (P > 0.05) between concentrate and RUP levels. Dry matter intake and nutrients digestibility was not affected (P > 0.05) by RUP level. Heifers fed the highest RUP level had greater (P < 0.05) average daily gain (ADG). Concentrate level did not affect (P > 0.05) DMI and ADG. Heifers fed diets with 80% concentrate had greater intake of TDN and EE, and lower intake of NDF (P < 0.05). The digestibilities of all nutrients, except the NDF, were greater (P < 0.05) for the 80% concentrate diets. The RUP levels did not affect (P > 0.05) the carcass characteristics and carcass gain composition. Heifers fed 80% concentrate diets had larger (P < 0.05) longissimus muscle area and lower gain of bones (P < 0.05). Concentrate level did not affect (P > 0.05) the composition of carcass gain. There was no effect (P > 0.05) of RUP and concentrate levels on meat quality traits and commercial cut yields.  相似文献   

Role of Endogenous Opioids for Regulation of the Oestrous Cycle in Sheep and Cattle     

PV Malven 《Reproduction in domestic animals》1995,30(4):183-187

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Neurological disorder in dairy cattle associated with consumption of beer residues contaminated with Aspergillus clavatus.     

Alexandre Paulino Loretti  Edson Moleta Colodel  David Driemeier  André Mendes Corrêa  Jorge José Bangel  Laerte Ferreiro 《Journal of veterinary diagnostic investigation》2003,15(2):123-132

A neurological syndrome in dairy cattle associated with consumption of moldy beer residues is described. The disease occurred on 1 farm in late June 2001, during winter. Six heifers and 1 cow out of 45 cattle were affected during a 3-week period. The affected animals died spontaneously or were euthanized approximately 2-14 days after the onset of clinical signs. The clinical signs were characterized by flaccid paralysis and gait abnormalities. Clinical signs were more pronounced after exercise and included stiff and unsteady gait, knuckling at the fetlocks of the hind limbs, frequent falling, inability to rise, muscular tremors, especially of the head and the hindquarters, and drooling. Main necropsy findings included degenerative and necrotic changes of the larger medial muscle groups of the hindquarters, i.e., adductor, pectineus, quadriceps femoris, rectus femuris, sartorius, semimembranosus, semitendinosus, and vastus medialis, and of the forequarters, including pectoralis descendens, pectoralis ascendens, and transversus pectoralis. The main histologic findings consisted of degenerative and necrotic neuronal changes (chromatolysis) of varying severity and extent affecting selected nuclei of the brainstem and neurons of the ventral horns of the spinal cord. Similar microscopic lesions were observed in the neurons of the spinal cord of 1 experimental sheep force-fed for 35 days with 1 kg/day of the same batch of foodstuff that was originally fed to the cattle. Coarse white or gray lumps, interpreted as mycelia, were observed in the beer by-product. Aspergillus clavatus was the dominant fungus isolated. Deaths ceased after the consumption of beer residue was discontinued. Recovery from illness was observed in 1 animal. The diagnosis was based on epidemiological data, clinical signs, necropsy findings, histological lesions, dosing trial, and mycology. A similar condition caused by consumption of barley by-products, sprouted wheat, corn sprouts, and beetroot screenings contaminated with A. clavatus has been reported in cattle and sheep worldwide.  相似文献   

Nutritional planning for Nellore heifers post-weaning to conception at 15 months of age: performance and nutritional,metabolic, and reproductive responses     

de Almeida  Daniel Mageste  Marcondes  Marcos Inácio  Rennó  Luciana Navajas  Pereira Silva  Luiz Henrique  Martins  Leandro Soares  Marquez  David Esteban Contreras  Castaño Villadiego  Faider Alberto  Velez Saldarriaga  Felipe  Franco  Julian David Castaño  Moreno  Deilen Paff Sotelo  de Moura  Felipe Henrique  Paulino  Mário Fonseca 《Tropical animal health and production》2019,51(1):79-87

Tropical Animal Health and Production - The objective of this study was to evaluate the effects of strategic supplementation in the dry period and dry/rainy transition period on the performance and...  相似文献   

Does supplementation of beef calves by creep feeding systems influence milk production and body condition of the dams?     

Sidnei Antônio Lopes  Mário Fonseca Paulino  Edenio Detmann  Ériton Egídio Lisboa Valente  Lívia Vieira de Barros  Luciana Navajas Rennó  Sebastião de Campos Valadares Filho  Leandro Soares Martins 《Tropical animal health and production》2016,48(6):1241-1246

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Current challenges in sheep artificial insemination: A particular insight     

Mercedes Alvarez  Luis Anel‐Lopez  Juan Carlos Boixo  Cesar Chamorro  Marta Neila‐Montero  Rafael Montes‐Garrido  Paulino de Paz  Luis Anel 《Reproduction in domestic animals》2019,54(Z4):32-40

Ovine artificial insemination (OAI) is not commonly performed because of specific problems related to semen application techniques, leading to highly variable results. The ideal methodology (frozen‐thawed semen/vaginal route) is unfeasible under field conditions due to the cervix morphology of the ewe, which prevents the process of intrauterine insemination necessary to obtain acceptable results. Currently, OAI commercial programmes use superficial cervical insemination, CAI (vaginal), with chilled semen (15°C) and intrauterine insemination, LAI (laparoscopic), with frozen‐thawed semen. The ability to improve upon these contrasting techniques may be derived from examining certain poorly studied factors such as insemination time, productive state of females and alternatives of seminal preservation, some of which we reviewed in this work. This interim solution will remain in use until AI by the vaginal route with frozen‐thawed semen is developed, but it poses new challenges in optimizing the freezing of the sperm and adapting the cervical (CAI) and/or transcervical intrauterine AI (TCAI). In this review, we address the current problems and evaluate their methodological (mechanical) and chemical (dilation) alternatives. Currently, TCAI is a methodologically complex technique with poor fertility results, so further studies are needed to improve the logistics of this procedure and the results of its application.  相似文献   

Soybean grain is a suitable replacement with soybean meal in multiple supplements for Nellore heifers grazing tropical pastures     

Daniel Mageste de Almeida  Marcos Inácio Marcondes  Luciana Navajas Rennó  Leandro Soares Martins  Faider Alberto Castaño Villadiego  Mário Fonseca Paulino 《Tropical animal health and production》2018,50(8):1843-1849

The research aimed to evaluate the effect of replacing soybean meal with soybean grain on the nutritional parameters and productivity of heifers grazing on Urochloa decumbens in the rainy-dry transition period. Forty crossbred heifers with the initial age and weight of 18 months and 292?±?6.1 kg, respectively, were used. The experimental design was a completely randomized design, with five treatments and eight replications. The evaluated treatments were as follows: SM_0.5—supply of 0.5 kg/animal/day of soybean meal supplement; SG_0.5—supply of 0.5 kg/animal/day of soybean grain supplement; SM_1.0—supply of 1.0 kg/animal/day of soybean meal supplement; SG_1.0—supply of 1.0 kg/animal/day of soybean grain supplement; MM—only mineral mix ad libitum. The supplement was isoprotein with 350 g of crude protein/kg of dry matter. Supplementation improved the performance of the heifers and this fact can be verified by the higher average daily gain (ADG) and final BW (fBW) of the supplemented animals (P?<?0.10). The two supplemented treatments with 1 kg/day demonstrated similar performance (P?>?0.10), the same happens for the two treatments receiving 0.5 kg/day (P?>?0.10). However, animals receiving 1 kg/day of supplementation had an ADG and final BW higher than animals receiving 0.5 kg/day (P?<?0.10). Supplementation (P?<?0.10) affected the intake of dry matter (DM), organic matter, crude protein, ether extract, non-fiber carbohydrates, total digestible nutrients, and neutral detergent fiber corrected for ash and protein (apNDF). Supplementation improved DM digestibility and all constituents of the diet (P?<?0.10), except for apNDF (P?>?0.10). In summary, it is concluded that multiple supplementations improve the performance of grazing heifers in the rainy-dry transition period and the total replacement of soybean meal by soybean grain does not alter the performance of the animals.  相似文献