Genome-wide association study for spot blotch resistance in Afghan wheat germplasm     

Naresh K. Bainsla  Rahul M. Phuke  Xinyao He  Vikas Gupta  Santosh K. Bishnoi  Rajiv K. Sharma  Najibeh Ataei  Susanne Dreisigacker  Philomin Juliana  Pawan K. Singh 《Plant pathology》2020,69(6):1161-1171

Spot blotch (SB), caused by Bipolaris sorokiniana, is a devastating disease of wheat globally, especially in South Asia and South America. Understanding the genetics of resistance to SB is important for developing breeding strategies to improve resistance. A panel of 301 genotypes from Afghanistan was phenotyped over two crop seasons using a mixture of virulent B. sorokiniana isolates and genotyped using DArTSeq to obtain genome-wide markers. Fifty genotypes (16.6%) showed disease scores less than the resistant control. Principal component analysis using the genotypic data clustered the genotypes into five different groups. Among models used for genome-wide association mapping, the multilocus mixed model, and fixed and random model circulating probability unification algorithms were most effective in identifying significant marker-trait associations (MTA). Twenty-five MTAs at p ≤ .001 were identified on chromosomes 1A, 1B, 1D, 2B, 2D, 3A, 3B, 4A, 5A, 5B, 6A, 7A, and 7D, indicating the quantitative nature of resistance to SB. Phenotypic variation explained by these markers ranged from 2.0% to 17.7%, and genomic regions on the chromosomes 1D, 2D, 3A, 3B, 4A, 5A, and 5B coincided with loci identified in previous studies. Three single nucleotide polymorphism (SNP) markers on chromosomes 1B (SNP 1113207) and 5A (SNPs 5411867 and 998276) were significant in both crop seasons as well as in the combined analysis across seasons. Marker 5411867 is close to Vrn-A1, shown to be associated with SB in previous studies. Furthermore, among known SB resistance genes, Sb2 on chromosome 5B was predicted to be significant in this panel.  相似文献   

Comparative productivity of Prosopis cineraria and Tecomella undulata based agroforestry systems in degraded lands of Indian Desert     

G. Singh 《林业研究》2009,20(2):144-150

Tree-crop interactions were monitored by measuring tree growth characters of Prosopis cineraria L. and Tecomella undulata L. and yields of Vigna radiata (L) in agroforestry systems in degraded lands of Indian Desert. Potential competition for resource between the trees and associated crop was analyzed by measuring soil water contents, soil organic matters and NH₄-N at different depths of soil layers i.e., 0–25 cm, 25–50 cm and 50–75 cm in the experimental plots. The plots size were 16 m × 18 m (D₁), 20 m × 18 m (D₂) and 32 m × 18 m (D₃) with tree densities of 208, 138 and 104 trees·ha⁻¹ after June 2002, respectively. Results showed that tree height increased by 3% to 7% during June 2002 to June 2004. Collar diameter increased by 30% and 11% in D₁, 23% and 19% in D₂ and 18% and 36% in D₃ plots, respectively, in P. cineraria and T. undulata in two years period. The increase in crown diameter was 9% to 18% in P. cineraria and 11% to 16% in T. undulata. Tree growth was relatively greater in 2002 than in 2003. Yield of V. radiata increased linearly from D₁ to D₃ plots. Lowest soil water content at 1 m distance from tree base indicated greater utilization of soil water within the tree rooting zone. Concentrations of soil organic matters and NH₄-N were the highest (p<0.05) in 0–25 cm soil layer. P. cineraria was more beneficial than T. undulata in improving soil conditions and increasing crop yield by 11.1% and thus more suitable for its integration in agricultural land. The yield of agricultural crop increased when density of tree species was appropriate (i.e., optimum tree density), though it varied with tree size and depended upon resource availability. The result indicated bio-economic benefits of optimum density of P. cineraria and T. undulata over traditional practices of maintaining random trees in farming system in arid zones. Biography: G. Singh (1961– ), male, Scientist E and Head, Division of Forest Ecology, Arid Forest Research Institute, New Pali Road, Jodhpur-342005, India.  相似文献   

Pigeonpea improvement: An amalgam of breeding and genomic research     

Sameer Kumar Chanda Venkata  Ganga Rao Nadigatla Veera Prabha Rama  Rachit K. Saxena  Kulbhushan Saxena  Hari D. Upadhyaya  Moses Siambi  Said N. Silim  Kothapally Narasimha Reddy  Anupama J. Hingane  Mamta Sharma  Shivali Sharma  Stephen Dominic Lyimo  Rose Ubwe  Meshack Makenge  Kananji Gad  Paul Kiprotich Kimurto  Manuel Amane  Kennedy Kanenga  Yuventino Obong  Emanuel Monyo  Chris Ojiewo  Nagesh Kumar Mallela Venkata  Jaganmohan Polineni Rao  Prashanthi Lakkireddy  Sudhakar Chourat  Indraprakash Singh  Sobhan Sajja  Shruthi Hirikara Beliappa  Rajeev K. Varshney 《Plant Breeding》2019,138(4):445-454

In the past five decades, constant research has been directed towards yield improvement in pigeonpea resulting in the deployment of several commercially acceptable cultivars in India. Though, the genesis of hybrid technology, the biggest breakthrough, enigma of stagnant productivity still remains unsolved. To sort this productivity disparity, genomic research along with conventional breeding was successfully initiated at ICRISAT. It endowed ample genomic resource providing insight in the pigeonpea genome combating production constraints in a precise and speedy manner. The availability of the draft genome sequence with a large‐scale marker resource, oriented the research towards trait mapping for flowering time, determinacy, fertility restoration, yield attributing traits and photo‐insensitivity. Defined core and mini‐core collection, still eased the pigeonpea breeding being accessible for existing genetic diversity and developing stress resistance. Modern genomic tools like next‐generation sequencing, genome‐wide selection helping in the appraisal of selection efficiency is leading towards next‐generation breeding, an awaited milestone in pigeonpea genetic enhancement. This paper emphasizes the ongoing genetic improvement in pigeonpea with an amalgam of conventional breeding as well as genomic research.  相似文献   

Inheritance and molecular mapping of restorer‐of‐fertility (Rf) gene in A2 hybrid system in pigeonpea (Cajanus cajan)     

Pankaj Sharma  Inderjit Singh  Asmita Sirari  Gaurav Khosla  Gurjeet Singh  Navkiran Kaur Ludhar  Sarvjeet Singh 《Plant Breeding》2019,138(6):741-747

Inheritance of fertility restorer gene in pigeonpea was studied using F₂ and BC₁F₁ populations derived from cross AL103A × IC245273. It was found to be controlled by single dominant gene. Out of 228 SSR primer pairs, 33 primer pairs showed parental polymorphism, while four primers were found polymorphic in bulk segregant analysis (BSA). These four primers viz., CcM 1615, CcM 0710, CcM 0765 and CcM 1522 were used for genotyping of F₂ population and were found to be placed at 3.1, 5.1, 28.1 and 45.8 cM, respectively. Two of them, CcM 1615 and CcM 0710, evinced clear and unambiguous bands for fertility restoration in F₂ population. The Rf gene was mapped on linkage group 6 between the SSR markers CcM 1615 and CcM 0710 with the distances of 3.1 and 5.1 cM, respectively. The accuracy of the CcM 1615 was validated in 18 restorers and six maintainer lines. The marker CcM 1615 amplified in majority of male restorer lines with a selection accuracy of 91.66%.  相似文献   

Preparation and Quality Evaluation of Nutritionally Enriched Jam Made from Blends of Indian Blackberry and Other Fruits     

Sourav Garg  Payel Ghosh  Sandeep Singh Rana 《International Journal of Fruit Science》2019,19(1):29-44

Jamun (Syzygium cumini) is a tropical, underutilized fruit which is highly perishable in nature. It is a good source of vitamin C, tannins, gallic acid and anthocyanins and its beneficial effects are mostly due to the presence of bioactive compounds (pigments and phenolic compounds) in it. Due to astringent and fibrous nature, preparation of jam from jamun pulp is quite difficult, but other fruits (apple and kiwifruit) can be incorporated in it to improve its quality. This study aims to develop jam from blends of jamun with other fruits and analyse various physico-chemical, nutritional, textural and sensory properties. It was found that physico-chemical properties of jams were not found to vary greatly, but the jamun–kiwifruit jam was found to have fairly high amount of antioxidants(46.75 ± 0.67%), tartaric acid (26.24 ± 0.02 mg/100g sample), ascorbic acid (0.08 ± 0.01 mg/100 g sample) and lactic acid (23.95 ± 0.01 mg/100g sample) and lowest amount of 5-hydroxymethyl-2-furaldehyde (0.38 ± 0.04 mg/100 g sample). Jamun jam and jamun–kiwifruit jam possessed the texture required for jam while jamun–apple jam was found to be a relatively harder gel. Jam made with jamun and kiwifruit pulp was found to have highest acceptability on the basis of sensory evaluation.  相似文献   

Grain yield,nitrogen use efficiency and tillage intensity in wheat as affected by precision nutrient management     

Jashanjot Kaur  Harinderjit Kaur  Pritpal Singh 《Communications in Soil Science and Plant Analysis》2020,51(11):1483-1498

ABSTRACT

The present studies were conducted to evaluate the effect of different nutrient management practices under two tillage options in wheat. The experiments were laid out in split-plot design with a combination of two varieties (WH 1105 and HD 2967) and two tillage options (Conventional and No tillage) in the main plot and six precision nutrient management practices [absolute control, site-specific nutrient management with Nutrient Expert for wheat (SSNM-NE)(170 kg nitrogen (N)/ha), SSNM NE+GreenSeeker (GS)(153/158 N kg/ha), N₁₂₀ (120 kg N/ha) before irrigation, N₁₂₀ after irrigation and N Rich (180 kg N/ha)] in subplot replicated thrice. The grain yield and quality characters in no tillage (NT) and conventional tillage (CT) were similar but agronomic efficiency was higher in NT. Both the varieties (WH 1105 and HD 2967) gave similar grain yield and quality. Wheat variety WH 1105 recorded significantly higher sodium dodecyl sulfate sedimentation (SDS) and gluten index. The treatment SSNM NE+GS had resulted in 107.1% higher grain yield than no nitrogen control but similar to enriched N plot (180 kg N/ha). The grain protein, SDS and gluten index in need-based nutrient management (SSNM+GS) treatment were found to be similar as recorded in SSNM-NE (170 kgN/ha) and N enriched plot (180 kg N ha^?1). The agronomic efficiency and recovery efficiency in SSNM+GS were also better than SSNM NE.  相似文献   

Soil and nutrients losses under different crop covers in vertisols of Central India     

Singh  R. K.  Chaudhary  R. S.  Somasundaram  J.  Sinha  N. K.  Mohanty  M.  Hati  K. M.  Rashmi  I.  Patra  A. K.  Chaudhari  S. K.  Lal  Rattan 《Journal of Soils and Sediments》2020,20(2):609-620

Purpose

Accelerated erosion removes fertile top soil along with nutrients through runoff and sediments, eventually affecting crop productivity and land degradation. However, scanty information is available on soil and nutrient losses under different crop covers in a vertisol of Central India. Thus, a field experiment was conducted for 4 years (2010–2013) to study the effect of different crop cover combinations on soil and nutrient losses through runoff in a vertisol.

Materials and methods

Very limited information is available on runoff, soil, and nutrient losses under different vegetative covers in a rainfed vertisol. Thus, the hypothesis of the study was to evaluate if different crop cover combinations would have greater impact on reducing soil and nutrient losses compared to control plots in a vertisol.

This experiment consisted of seven treatment combinations of crop covers namely soybean (Glycine max) (CC₁), maize (Zea mays) (CC₂), pigeon pea (Cajanus cajan) (CC₃), soybean (Glycine max)?+?maize (Zea mays) ??1:1 (CC₄), soybean (Glycine ma x))?+?pigeon pea (Cajanus cajan) ?2:1 (CC₅), maize (Zea mays)?+?pigeon pea (Cajanus cajan) ??1:1 (CC₆), and cultivated fallow (CC₇). The plot size was 10?×?5 m with 1% slope, and runoff and soil loss were measured using multi-slot devisor. All treatments were arranged in a randomized block design with three replications.

Results and discussion

Results demonstrated that the runoff and soil loss were significantly (p?<?0.05) higher (289 mm and 3.92 Mg ha^?1) under cultivated fallow than those in cropped plots. Among various crop covers, sole pigeon pea (CC₃) recorded significantly higher runoff and soil loss (257 mm and 3.16 Mg ha^?1) followed by that under sole maize (CC₂) (235 mm and 2.85 Mg ha^?1) and the intercrops were in the order of maize?+?pigeon pea (211 mm and 2.47 Mg ha^?1) followed by soybean?+?maize (202 mm and 2.38 Mg ha^?1), and soybean?+?pigeon pea (195 mm and 2.15 Mg ha^?1). The lowest runoff and soil loss were recorded under soybean sole crop (194 mm and 2.27 Mg ha^?1). The data on nutrient losses indicated that the highest losses of soil organic carbon (SOC) (25.83 kg ha^?1), total nitrogen (N), phosphorus (P), and potassium (K) (7.76, 0.96, 32.5 kg ha^?1) were recorded in cultivated fallow (CC₇) as compared to those from sole and intercrop treatments. However, sole soybean and its intercrops recorded the minimum losses of SOC and total N, P, and K, whereas the maximum losses of nutrients were recorded under pigeon pea (CC₃). The system productivity in terms of soybean grain equivalent yield (SGEY) was higher (p?<?0.05) from maize?+?pigeon pea (3358 kg ha^?1) followed by that for soybean?+?pigeon pea (2191 kg ha^?1) as compared to sole soybean. Therefore, maize?+?pigeon pea (1:1) intercropping is the promising option in reducing runoff, soil-nutrient losses, and enhancing crop productivity in the hot sub-humid eco-region.

Conclusions

Study results highlight the need for maintenance of suitable vegetative cover as of great significance to diffusing the erosive energy of heavy rains and also safe guarding the soil resource from degradation by water erosion in vertisols.

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Biocontrol potential of Pseudomonas azotoformans,Serratia marcescens and Trichoderma virens against Fusarium wilt of Dalbergia sissoo     

Sabyasachi Banerjee  Seema Singh  Shailesh Pandey  Maneesh S. Bhandari  Amit Pandey  Krishna Giri 《Forest Pathology》2020,50(2)

Wilt disease caused by Fusarium solani is a serious constraint to Dalbergia sissoo (shisham) plantations in northern India. In this study, the antagonistic potential of 40 bacterial isolates recovered from rhizophere soil of healthy shisham trees, and a well‐characterized Trichoderma species (Trichoderma virens) were tested for their possibility as biocontrol agents for F. solani. Two promising isolates (S1 and S15) were identified which inhibited pathogen growth, caused chitin degradation, produced siderophores and solubilized phosphate in vitro. Isolate S15 scored highest for hydrogen cyanide (HCN) production while isolate S1 was a non‐HCN producer. These two isolates were identified as Serratia marcescens (S1) and Pseudomonas azotoformans (S15) following sequence analysis of 16S rDNA. In dual culture assays, T. virens caused 80% inhibition of mycelial growth of the test fungus. The three selected antagonists when tested in planta in the glasshouse completely suppressed production of wilt symptoms on 12‐month‐old shisham plants. Further work is needed to ascertain the potential of these isolates to be used as biocontrol agents to manage shisham wilt under field conditions.  相似文献   

Confirmation of organothiophosphorus insecticide residues in fruit and vegetables by oxidative derivatization.     

J Singh  W P Cochrane 《Journal of the Association of Official Analytical Chemists》1979,62(4):751-756

Oxidative derivatization of 10 organothiophosphorus insecticides at nanogram levels, with neutralized sodium hypochlorite (NaOCl) produced their respective P=O oxygen analogs, as shown by GC/MS and FT-IR analysis.In addition, phorate resulted in phorate oxon sulfoxide while methidathion gave a mixture of oxidation products. Four macro-scale oxidation products of methidathion were isolated by thin layer chromatography and 3 were identified, namely, methidaoxon, bis(2-methoxy-delta2-1,3,4-thiadiazolin-5-on-4-yl) sulfide, and the corresponding disulfide, by comparison of their infrared and mass spectra with those of authentic samples. The relative response to sulfide and disulfide products of the flame photometric detector in both the P- and S-modes is discussed. This derivatization is applied to the confirmation of chlorpyrifos, ethion, phorate, DMPA (Zytron), leptophos, and methidathion in celery, potatoes, lettuce, tomatoes, and apples at 0.25-0.5 ppm fortification levels.  相似文献   

Laboratory study of phosphorus forms and test procedures on soils under aerobic and anaerobic conditions     

R.K. Gupta  T.A. Singh 《Geoderma》1975,14(3):255-260

The inorganic forms of phosphorus in nine samples of surface soils from the Nainital Tarai of India were determined by a series of extractions with different reagents after incubation for 45 days under aerobic or anaerobic conditions. The forms of phosphorus in samples incubated under aerobic conditions and the pH range of 7.2–8.5 indicated that the soils had been subject to slight chemical weathering. Anaerobic incubation to simulate conditions in lowland rice paddies converted part of the inorganic phosphorus into more labile forms, based on changes in amounts of isotopically exchangeable phosphorus. Amounts of the element converted to more labile forms differed among the nine soil samples.A better correlation was found between amounts of phosphorus extracted by 0.5M NaHCO³ (Olsen's method) than by 0.03N NH₄F in 0.25N HCl (Bray's method) and the isotopically exchangeable phosphorus. The better correlation suggests that the bicarbonate extraction would be the better method for estimating “availablelrd phosphorus in soils of lowland rice paddies. Recalibration of the test prior to such use seems desirable to allow for increases in amounts of phosphorus in labile forms after soils have been shifted from aerobic to anaerobic conditions.  相似文献