Biological and molecular characterization of a novel carmovirus isolated from angelonia     

Adkins S  Hammond J  Gera A  Maroon-Lango CJ  Sobolev I  Harness A  Zeidan M  Spiegel S 《Phytopathology》2006,96(5):460-467

ABSTRACT A new carmovirus was isolated from Angelonia plants (Angelonia angustifolia), with flower break and mild foliar symptoms, grown in the United States and Israel. The virus, for which the name Angelonia flower break virus (AnFBV) is proposed, has isometric particles, approximately 30 nm in diameter. The experimental host range was limited to Nicotiana species, Schizanthus pinnatus, Myosotis sylvatica, Phlox drummondii, and Digitalis purpurea. Virions were isolated from systemically infected N. benthamiana leaves, and directly from naturally infected Angelonia leaves, using typical carmovirus protocols. Koch's postulates were completed by mechanical inoculation of uninfected Angelonia seedlings with purified virions. Isometric particles were observed in leaf dips and virion preparations from both Angelonia and N. benthamiana, and in thin sections of Angelonia flower tissue by electron microscopy. In sodium dodecyl sulfate-polyacrylamide gel electrophoresis of dissociated purified virus preparations, a major protein component with a molecular mass of 38 kDa was observed. Virion preparations were used to produce virus-specific polyclonal antisera in both Israel and the United States. The antisera did not react with Pelargonium flower break virus (PFBV), Carnation mottle virus (CarMV), or Saguaro cactus virus (SgCV) by either enzyme-linked immunosorbent assay or immunoblotting. In reciprocal tests, antisera against PFBV, CarMV, and SgCV reacted only with the homologous viruses. The complete nucleotide sequence of a Florida isolate of AnFBV and the coat protein (CP) gene sequences of Israeli and Maryland isolates were determined. The genomic RNA is 3,964 nucleotides and contains four open reading frames arranged in a manner typical of carmoviruses. The AnFBV CP is most closely related to PFBV, whereas the AnFBV replicase is most closely related to PFBV, CarMV, and SgCV. Particle morphology, serological properties, genome organization, and phylogenetic analysis are all consistent with assignment of AnFBV to the genus Carmovirus.  相似文献   

The experimental transfer of immature Fasciola hepatica from donor mice and hamsters to rats immunised against the donors     

D L Hughes  E Harness 《Research in veterinary science》1973,14(2):220-222

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Livestock exports--an ethical debate     

Paxton D 《Australian veterinary journal》2004,82(3):134

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Export and reception of livestock in Egypt     

Paxton D 《Australian veterinary journal》2003,81(7):394

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Blood transfusion and red cell survival in dizygotic cattle twins     

W M Fitzsimmons  B F Sansom  J G Hall  J S Stewart  S A Sellwood  E Harness 《The British veterinary journal》1967,123(9):397-402

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The experimental transfer of adult Fasciola hepatica between various mammalian hosts   总被引：1，自引：0，他引：1  

D L Hughes  E Harness 《Research in veterinary science》1972,13(5):422-426

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The effect of temperature on growth and dry matter production of lychee seedlings     

C.M. Menzel  B.F. Paxton 《Scientia Horticulturae》1985,26(1):17-23

In lychee (Litchi chinensis Sonn.), high day/night temperatures ($\text{30}\text{25}$, $\text{25}\text{20}$, $\text{20}\text{15}\text{°}\text{C}$ compared to $\text{15}\text{10}\text{°}\text{C}$) increased stem extension, node and leaf production and plant dry weight in seedling selections of 6 cultivars (‘Bengal’, ‘Haak Yip’, ‘Kwai May’, ‘Gee Kee’, ‘Tai So’ and ‘Wai Chee’). The mean daily base temperature for shoot growth (node production) was estimated by linear correlation to be 11.0°C for the seedlings. High temperatures also increased the proportion of leaf and decreased the proportion of root in the total dry weight. There was a strong effect of cultivar on all aspects of growth, but the responses to temperature were similar.This experiment shows that temperatures in many tropical and warm sub-tropical areas where lychees are grown are too high to induce satisfactory vegetative dormancy prior to floral initiation. The assessment of lychee seedlings for low vigour in a heated glasshouse appears to be a useful technique for the initial screening of adapted genotypes (which flower and fruit under warm conditions) prior to field evaluation.  相似文献   

Experimental Trichostrongylus colubrigormis infection in adult goats     

W M Fitzsimmons  E Harness  S A Sellwood 《Research in veterinary science》1968,9(3):237-247

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后水河水库大坝的稳定分析     

王耐力  杨卫中 《中国农村水利水电》1996,(6):36-38

对后水河水库大坝进行了分析，在坝体不设置伸缩缝，且坝基与基岩的结合处理较好时，大坝抗滑稳定属整体抗滑稳定问题，并可采用抗剪断公式进行计算及核核，可以减小坝体断面，有一定的经济效益。  相似文献   

Creep in chipboard     

J. M. Dinwoodie  J. -A. Higgins  B. H. Paxton  D. J. Robson 《Wood Science and Technology》1991,25(5):383-396

Summary Samples of five chipboards, one waferboard, one plywood, one fibre building board and redwood timber (Pinus sylvestris) were loaded in 4-point bending under four stress levels: 30, 45, 67.5 and 75% of the short term ultimate, thereby extending earlier work at 60% stress level. Five environmental conditions were used at each stress level: 10°C 62% relative humidity (rh); 20°C 30% rh; 20 °C 65% rh; 20°C 90% rh and 30°C 68% rh. Deflections were recorded at least on a daily basis for a period of 6 months, unless failure of the sample occurred earlier. Values for the elastic, viscoelastic and viscous components of creep deflection were calculated for two time periods using the 4-element rheological model developed in an earlier paper: comparisons were made on the basis of the percentage contribution of each component. The relative proportions of the elastic, viscoelastic and viscous components making up total deflection were found to vary considerably with time, temperature, relative humidity, stress level and material. The relationships were complex with strong interactions occurring among environmental condition, stress level and material. Thus, for example, the effect of high temperature, or high relative humidity, in determining the magnitude of the viscous component was much greater at higher levels of stressing: waferboard appeared to be less sensitive to high humidity and solid timber more sensitive to high temperature than the other materials. T-tests further confirmed the complexity of the relationships. It is concluded that much of the variability in creep deflection recorded in Part 8 of this series is primarily due to changes in the percentage contribution of the viscous component.  相似文献