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101.
A 5-year-old, neutered male Domestic Short-haired cat was referred with a 5-month history of anterior uveitis and cataract in the right eye. Clinical examination confirmed anterior uveitis and immature cataract in the right eye and chorioretinitis in the left eye. Ocular ultrasound showed a retinal detachment in the right eye. Diagnostic testing revealed elevated serum titers for Toxoplasma gondii. Anterior uveitis in the right eye and chorioretinitis in the left eye progressed, resulting in blindness despite a 21-day course of clindamycin and aggressive topical medical management of uveitis. The right eye was enucleated and histopathologic evaluation of the globe revealed panuveitis and multiple organisms morphologically consistent with Histoplasma capsulatum. Systemic treatment with itraconazole was initiated. Vision returned after 3 months of treatment and complete resolution of the retinal hemorrhages with formation of a flat chorioretinal scar was noted after 6 months of therapy. Itraconazole was discontinued 7 months after starting therapy, at which time the funduscopic appearance of the chorioretinal scar had remained static for 1 month. The cat has remained visual without evidence of disease progression for 6 months following discontinuation of itraconazole. 相似文献
102.
Barth CA Fastie WG Hord CW Pearce JB Kelly KK Stewart AI Thomas GE Anderson GP Raper OF 《Science (New York, N.Y.)》1969,165(3897):1004-1005
Emission features from ionized carbon dioxide and carbon monoxide were measured in the 1900- to 4300-angstrom spectral region. The Lyman alpha 1216-angstrom line of atomic hydrogen and the 1304-, 1356-, and 2972-angstrom lines of atomic oxygen were observed. 相似文献
103.
The magnetic properties of samples of rock, fines, and magnetic separate from the fines from Apollo 11 have been measured. Native iron, or possibly nickel-iron, of submicroscopic particle size is the most important constituent, with minor contributions from ilmenite, paramagnetic iron minerals, and other iron-titanium oxides. The remanent magnetization of a sample of the micro-breccia rapidly acquires a viscous magnetization and does not appear to have a significant stable remanence. The crystalline sample has a weak natural remanence showing some stability. 相似文献
104.
105.
Creamer LK Pearce LE Hill JP Boland MJ 《Journal of agricultural and food chemistry》2002,50(25):7187-7193
Dairying into the 21st century will largely continue with the trends seen in the past few decades, although there is always the possibility of an unlikely but disruptive event. The politics of globalization will potentially be important in freeing up global trade in dairy products. Production on the farm will become increasingly efficient, resulting in continuing price benefits to the consumer. At the same time, increasing attention will be paid by the consumer, producer, and manufacturer to safety and quality issues. Environmental concerns will increase in importance, and the issue of methane production may be important for the industry over the next two decades. It is unlikely that genetically modified milk will be introduced soon, even if public acceptance ceases to be an issue; however, the use of genetic markers for accelerated genetic improvement of cows will have rapidly increasing importance. Despite increasing pressure from nonmilk alternatives, milk and dairy will still be the best sources of nutrition for the young and for traditional dairy products. Consumer concerns will be of overriding importance for the industry, and the safety of dairy foods must become absolute. Recent advances in the chemical, physical, and information sciences and technologies will be utilized to gain greater understanding of the increasingly complex food systems and to support the consumer objectives. 相似文献
106.
Variability in the concentration of three heat stable proteinase inhibitor proteins in potato tubers
The variability of three well characterized proteinase inhibitors, Inhibitor I, molecular weight 39,000, Inhibitor II, molecular weight 21,000, and Carboxypeptidase Inhibitor, molecular weight 4,100, were determined in apical cortical tissues of individual potato tubers of the Russet Burbank variety. The three inhibitors varied within ± 20% among sixty-five tubers and cumulatively represented about 7% of the total soluble proteins. The inhibitors were highly variable among tubers of 106 clones from randomly chosen varieties. Inhibitor I varied about twelve-fold (60 to 745 μg/ml juice), and Inhibitor II varied about seven-fold (158 to 1,025 μg/ml juice). Carboxypeptidase Inhibitor varied from as low as zero (seven varieties) to over 850 μg/ml tuber juice. With 80 tubers from fourteen varieties of potatoes, a positivecorrelation was found between the concentrations of Inhibitor I and Inhibitor II and total soluble protein. Carboxypeptidase Inhibitor did not correlate well with total soluble protein. The positive correlations of Inhibitors I and II (a correlation coefficient of 0.70) with total soluble protein indicated that the proteinase inhibitors may be excellent markers for genetic studies for selecting high protein potato tuber varieties. 相似文献
107.
Laurent Calcul Carrie Waterman Wai Sheung Ma Matthew D. Lebar Charles Harter Tina Mutka Lindsay Morton Patrick Maignan Alberto Van Olphen Dennis E. Kyle Lilian Vrijmoed Ka-Lai Pang Cedric Pearce Bill J. Baker 《Marine drugs》2013,11(12):5036-5050
We conducted a screening campaign to investigate fungi as a source for new antimalarial compounds. A subset of our fungal collection comprising Chinese mangrove endophytes provided over 5000 lipophilic extracts. We developed an accelerated discovery program based on small-scale cultivation for crude extract screening and a high-throughput malaria assay. Criteria for hits were developed and high priority hits were subjected to scale-up cultivation. Extracts from large scale cultivation were fractionated and these fractions subjected to both in vitro malaria and cytotoxicity screening. Criteria for advancing fractions to purification were developed, including the introduction of a selectivity index and by dereplication of known metabolites. From the Chinese mangrove endophytes, four new compounds (14–16, 18) were isolated including a new dimeric tetrahydroxanthone, dicerandrol D (14), which was found to display the most favorable bioactivity profile. 相似文献
108.
David Sutton Carina Vinberg Agneta Gustafsson Jacqueline Pearce Neil Greenwood 《Acta veterinaria Scandinavica》2013,55(1):64
A litter of recently-vaccinated puppies in Sweden experienced signs of severe haemorrhagic gastroenteritis. Canine parvovirus (CPV) was suspected as the cause of this outbreak on the basis of the clinical signs and the presence of parvoviral antigen in the faeces from one of the affected pups - confirmed using a commercial in-clinic faecal antigen ELISA test kit. A concern was raised about whether the vaccine (which contained a live, attenuated strain of CPV) could have caused the disease and so further faecal samples from the affected pups were submitted for laboratory virus isolation and identification.On cell culture, two out of four faecal samples were found to be virus-positive. This was confirmed as being canine parvovirus by immuno-staining with CPV specific monoclonal antibody. The virus was then tested using a series of PCR probes designed to confirm the identity of CPV and to distinguish the unique vaccine strain from field virus. This confirmed that the virus was indeed CPV but that it was not vaccine strain. The virus was then typed by sequencing the 426 amino acid region of the capsid gene which revealed this to be a type 2c virus.Since its emergence in the late 1970s, canine parvovirus 2 (CPV2) has spread worldwide and is recognised as an important canine pathogen in all countries. The original CPV2 rapidly evolved into two antigenic variants, CPV2a and CPV2b, which progressively replaced the original CPV2. More recently a new antigenic variant, CPV2c, has appeared. To date this variant has been identified in many countries worldwide but there have been no reports yet of its presence in any Scandinavian countries. This case report therefore represents the first published evidence of the involvement of CPV2c in a severe outbreak of typical haemorrhagic gastroenteritis in a susceptible litter of pups in Scandinavia. 相似文献
109.
Helen Gurney‐Smith Nadia Plamondon Simon Yuan Christopher M Pearce 《Aquaculture Research》2013,44(8):1236-1253
The basket cockle (Clinocardium nuttallii) is a candidate species for aquaculture in the northeast Pacific. The aim of the current research was to assess the feasibility of C. nuttallii grow‐out, with an emphasis on growth performance and qualities affecting product marketability. In this article, we investigated the combined effects of culture mode (intertidal and off‐bottom suspended culture) and initial stocking density (1500, 3000, 10 500 and 21 000 ind m?2) on C. nuttallii survival and growth during the first year of grow‐out (May through October). In intertidal culture, cockles exhibited low survival and poor growth rates. In suspended culture, survival was consistently high (>96%) at all stocking densities tested; growth and condition parameters had the highest values at 1500 and 3000 ind m?2. The edible portion (meat yield) exceeded 40% of the whole wet weight at all stocking densities, occurrences of fouled and deformed cockles were <1% and no commensal species were observed. Depending on the minimum harvestable size and stocking density chosen, harvestable proportions constituted from 1.1% to 15.2% by October of the first grow‐out year in the suspended system. The effects of stocking density and depth on second year grow‐out performance of C. nuttallii are reported in a companion paper (Dunham et al. in this issue). 相似文献
110.
Helen Gurney‐Smith Nadia Plamondon Simon Yuan Christopher M Pearce 《Aquaculture Research》2013,44(8):1277-1299
We investigated the effects of culture mode (intertidal and off‐bottom suspended culture) and initial stocking density (5%, 10%, 35% and 70% bottom cover) on survival, growth and condition indices of three size cohorts (16, 22 and 32 mm initial shell length) of the basket cockle, Clinocardium nuttallii, during a second year of grow‐out. In addition, we evaluated the effects of depth (2, 4 and 6 m) in suspended culture. All size cohorts demonstrated significantly higher growth rates, soft‐tissue biomass and condition indices in the off‐bottom suspended system compared to the intertidal one. In suspended culture, however, C. nuttallii exhibited sub‐optimal survival, shell deformities and heavy fouling. For all size cohorts, stocking density had a significant effect on most growth and condition parameters, except meat yield indices, in both culture modes. An initial maximum stocking density of 10% cover is recommended for all size cohorts. Culture depth did not have a consistent statistically significant effect on any of the growth or condition parameters. Depending on the grow‐out scenario, stocking density and harvestable size chosen, the cumulative harvestable proportion after the second year of grow‐out constituted 15.5–63.1% of the seed planted. Our results could be used to develop and improve culture techniques for C. nuttallii and other cockle species. 相似文献