Assessment of the uniformity of wheat and tomato varieties at DNA microsatellite loci   总被引：9，自引：1，他引：9  

R.J. Cooke  G.M.M. Bredemeijer  M.W. Ganal  R. Peeters  P. Isaac  S. Rendell  J. Jackson  M.S. Röder  V. Korzun  K. Wendehake  T. Areshchenkova  M. Dijcks  D. Laborie  L. Bertrand  B. Vosman 《Euphytica》2003,132(3):331-341

By analysing a number (20–38) of individuals from selected varieties of wheat and tomato, we have been able to assess intra-varietal uniformity at certain micro satellite (simple sequence repeat, SSR) loci. In total, 45 varieties of wheat were analysed at between 7–9 different SSR loci, and 10 varieties of tomato were analysed at six loci. The results showed that there was variation both between varieties and between microsatellites in the degree of non-uniformity observed, and it was possible to identify a number of different probable sources of non-uniformity. Twenty-four of the wheat varieties and nine of the tomato varieties were sufficiently uniform to meet the standards currently applied for distinctness, uniformity and stability (DUS) testing using phenotypic characteristics. The implications for the potential future use of SSRs in DUS testing are discussed. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Gender differences in academic rank among faculty surgeons at US veterinary schools in 2019     

Michelle A. Giuffrida VMD  MSCE  DACVS-SA  Michele A. Steffey DVM  DACVS-SA  Ingrid M. Balsa DVM  ME  DACVS-SA  Samantha L. Morello DVM  DACVS-LA  Amy S. Kapatkin DVM  MS  DACVS 《Veterinary surgery : VS》2020,49(5):852-859

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Comparison of surgical variables and short-term postoperative complications in healthy dogs undergoing ovariohysterectomy or ovariectomy     

Peeters ME  Kirpensteijn J 《Journal of the American Veterinary Medical Association》2011,238(2):189-194

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Idiopathic chylothorax and lymphedema in 2 whippet littermates     

Schuller S  Le Garrérès A  Remy I  Peeters D 《The Canadian veterinary journal. La revue veterinaire canadienne》2011,52(11):1243-1245

Idiopathic chylothorax and limb edema was diagnosed in two 2-year-old male whippet siblings. The fact that the 2 related animals developed similar clinical signs at a young age may suggest a congenital or hereditary etiology.  相似文献   

Pathogen group specific risk factors at herd, heifer and quarter levels for intramammary infections in early lactating dairy heifers     

Piepers S  Peeters K  Opsomer G  Barkema HW  Frankena K  De Vliegher S 《Preventive veterinary medicine》2011,99(2-4):91-101

Risk factors for intramammary infections caused by coagulase-negative staphylococci, contagious major pathogens and environmental major pathogens in early lactating heifers were evaluated at the herd, heifer and quarter levels. In total, 764 quarters of 191 dairy heifers in 20 randomly selected farms in Flanders (Belgium) were sampled. Quarter milk samples were collected between 1 and 4 days in milk and between 5 and 8 days in milk for bacteriological culture. Data were analyzed using multivariable, multilevel logistic regression analysis. Higher average herd milk somatic cell count (>200,000 cells/mL), not having an effective fly control strategy, contact with lactating cows prior to calving and moderate to severe udder edema prior to calving increased the odds of intramammary infections caused by contagious major pathogens. Poor heifer hygiene and lack of mineral/vitamin supplementation prior to calving were risk factors for intramammary infection caused by environmental major pathogens. Teat apex colonization with coagulase-negative staphylococci prior to calving seemed to protect quarters against intramammary infections caused by major pathogens. Poor heifer hygiene before calving, a non-clipped udder and not practicing of teat dipping prior to calving increased the odds of intramammary infection with coagulase-negative staphylococci. Although management is important in the prevention and control of intramammary infections in early lactating heifers, most variation in the prevalence of intramammary infections resided at the heifer and quarter levels, indicating that the susceptibility for intramammary infections around calving is mainly determined by heifer and quarter characteristics.  相似文献   

Expression of Maternal Transcripts During Bovine Oocyte In Vitro Maturation is Affected by Donor Age     

R Romar  T De Santis  P Papillier  C Perreau  A Thélie  ME Dell’Aquila  P Mermillod  R Dalbiès‐Tran 《Reproduction in domestic animals》2011,46(1):e23-e30

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Scanning and transmission electron microscopy of attaching effacing Escherichia coli in weanling rabbits   总被引：7，自引：0，他引：7  

J E Peeters  G J Charlier  R Raeymaekers 《Veterinary pathology》1985,22(1):54-59

A strain of an enteropathogenic Escherichia coli, originally isolated from diarrheic weaned rabbits, produced diarrhea in five-week-old New Zealand white rabbits. Sequential examination of the intestines by scanning and transmission electron microscopy revealed that the strain attaches first to the Peyer's patch dome epithelium and later to the enterocytes of distal small intestine, cecum, and colon. Colonized cells became rounded and detached. The colibacilli were intimately associated with the apical cell membrane. Both absorptive and goblet cells were affected. The strain caused effacement of the microvillous border of colonized epithelial cells. Colibacilli were regularly seen in the partially evacuated cavities of goblet cells, but not in absorptive epithelial cells.  相似文献   

Development and application of multiple internal reference (housekeeper) gene assays for accurate normalisation of canine gene expression studies     

Peters IR  Peeters D  Helps CR  Day MJ 《Veterinary immunology and immunopathology》2007,117(1-2):55-66

Measurement of mRNA expression by real-time RT-PCR (QRT-PCR) has proven to be an important and powerful tool for the investigation of the pathogenesis of inflammatory and immune-mediated diseases in many species. This methodology has proven particularly valuable in the dog, a species for which there are currently few specific antibodies for measurement of relevant proteins. Internal control (housekeeper) mRNAs are widely used for normalisation of QRT-PCR results. The validation and use of multiple internal control mRNAs for increased accuracy of normalisation has been described for humans and rodents. The aims of this study were to develop QRT-PCR assays for 11 potential internal control mRNAs in the dog (ACTB, B(2)M, G3PDH, HMBS, HPRT1, RPL13A, RPL32, RPS18, SDHA, TBP and YWAZ) and validate their use with bone marrow, colon, duodenum, heart, kidney, liver, lung, lymph node, skeletal muscle, pancreas, spleen and stomach from seven dogs. Endoscopic biopsies of the superficial duodenal mucosa were also obtained from nine dogs suffering from chronic gastro-oesophageal disease. The most stably expressed genes varied in the tissues examined. RPL13A and RPL32 (both components of the 60S ribosomal subunit) were the most stably expressed genes in the majority of the tissues examined, whereas ACTB and B(2)M were the least stable. Distinct internal control genes were shown to be most appropriate for use in full-thickness versus superficial mucosal biopsies of the duodenum. The results of this study indicate that there are no universal control genes for gene expression studies in canine tissues. It is important to use multiple internal control genes based upon a survey of potential control genes applied to representative samples from different disease groups, culture conditions and/or time points in an experimental study.  相似文献   

Update on canine sinonasal aspergillosis.     

Dominique Peeters  Cécile Clercx 《Veterinary Clinics of North America: Small Animal Practice》2007,37(5):901-16, vi

Sinonasal aspergillosis is a frequent cause of nasal discharge that occurs in otherwise healthy, young to middle-aged dogs. A local immune dysfunction is suspected in affected animals, and the role of increased interleukin-10 mRNA expression in the nasal mucosa of affected dogs is currently under investigation. Despite recent advances in imaging techniques, the "gold standard" for diagnosing the disease is direct visualization of fungal plaques during endoscopy or observation of fungal elements on cytology or histopathologic examination. Treatment can be challenging; however, the use of topical enilconazole or clotrimazole through noninvasive techniques has increased the success of treatment and decreased the morbidity and duration of hospitalization.  相似文献   

Susceptibility to antimicrobials of mastitis‐causing Staphylococcus aureus,Streptococcus uberis and Str. dysgalactiae from New Zealand and the USA as assessed by the disk diffusion test          下载免费PDF全文

KR Petrovski  A Grinberg  NB Williamson  ME Abdalla  N Lopez‐Villalobos  TJ Parkinson  IG Tucker  P Rapnicki 《Australian veterinary journal》2015,93(7):227-233

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