Pilot study of Australian veterinarians and their perceptions and experiences related to online pet health information     

LR Kogan  JA Oxley  SJ Hazel 《Australian veterinary journal》2019,97(1-2):10-13

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Retrospective evaluation of the efficacy of minimally invasive,fluoroscopic‐assisted reduction and stabilisation of unicondylar humeral fractures          下载免费PDF全文

JA Au Yong  DD Lewis  RB Evans  SE Kim  A Pozzi 《Australian veterinary journal》2018,96(8):302-307

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Competitive effects between rainbow trout and Atlantic salmon in natural and artificial streams          下载免费PDF全文

Aimee Lee S. Houde  Andrew D. Smith  Chris C. Wilson  Pedro R. Peres‐Neto  Bryan D. Neff 《Ecology of Freshwater Fish》2016,25(2):248-260

Competition with non‐native species may impede the restoration of native species, but differences in competitive abilities among intraspecific native populations may make some populations more suitable for reintroduction than others. Here, juvenile Atlantic salmon (Salmo salar) from two allopatric populations (LaHave and Sebago) being used for reintroduction into Lake Ontario were placed into two natural stream sites differing in the presence of ecologically similar rainbow trout (Oncorhynchus mykiss). We assessed the effects of competition in the natural streams on fitness‐related traits and habitat use of the Atlantic salmon. We then compared these effects to those observed in artificial streams from a previous study. Atlantic salmon in natural streams had reduced fitness‐related traits that were associated with suboptimal microhabitats in the presence of rainbow trout, but utilised optimal microhabitats in their absence. In the presence of rainbow trout, the two Atlantic salmon populations exhibited comparable recapture proportions to each other, but the individuals from the Sebago population had better performance (body size and condition) than those from the LaHave population. Responses of both Atlantic salmon populations to competition with rainbow trout were generally similar in both direction and magnitude when compared to results from the artificial stream study. The combined results suggest that native species restoration efforts should be focused on candidate populations that are ecologically suitable to reintroduction environments, as well as on suitable habitats that do not contain exotic competitors. Moreover, this study highlights the value of controlled experiments in artificial environments for predicting fitness‐related performance in natural environments.  相似文献   

Selection for Pathogenicity to Strawberry in Populations of Colletotrichum gloeosporioides from Native Plants     

Mackenzie SJ  Seijo TE  Legard DE  Timmer LW  Peres NA 《Phytopathology》2007,97(9):1130-1140

ABSTRACT Colletotrichum gloeosporioides causes a serious crown rot of strawberry and some isolates from native plants are pathogenic to strawberry. C. gloeosporioides from lesions on wild grape and oak were sampled at two sites adjacent to commercial strawberry fields in Florida and two distant sites. Random amplified polymorphic DNA (RAPD) marker data and restriction enzyme digests of amplified rDNA were used to determine whether isolates were from the same C. gloeosporioides subgroup that infects strawberry. There were 17 to 24 native host isolates from each site that clustered with a group of strawberry crown isolates based on RAPD markers. Among strawberry isolates, there were two rDNA genotypes identified by restriction enzyme analysis. Both genotypes were present among native host isolates sampled from all four sites. There was some evidence that the different rDNA genotypes differentiated two closely related subpopulations, although the proportion of pathogenic isolates from native hosts among the two different genotypes was not different. The incidence of isolates pathogenic to strawberry was greater at sites close to strawberry fields relative to sites distant from strawberry fields for isolates with a BstUI(-)/MspI(+) rDNA genotype (44 versus 13%), a BstUI(+)/MspI(-) genotype (57 versus 16%), or when both genotypes were analyzed together (46 versus 15%). Based on these results, it appears that the C. gloeosporioides subgroup that causes crown rot on strawberry is widely distributed in Florida and that selection for pathogenicity on strawberry occurs in the area where this host is grown in abundance.  相似文献   

Global network reorganization during dynamic adaptations of Bacillus subtilis metabolism     

Buescher JM  Liebermeister W  Jules M  Uhr M  Muntel J  Botella E  Hessling B  Kleijn RJ  Le Chat L  Lecointe F  Mäder U  Nicolas P  Piersma S  Rügheimer F  Becher D  Bessieres P  Bidnenko E  Denham EL  Dervyn E  Devine KM  Doherty G  Drulhe S  Felicori L  Fogg MJ  Goelzer A  Hansen A  Harwood CR  Hecker M  Hubner S  Hultschig C  Jarmer H  Klipp E  Leduc A  Lewis P  Molina F  Noirot P  Peres S  Pigeonneau N  Pohl S  Rasmussen S  Rinn B  Schaffer M  Schnidder J  Schwikowski B  Van Dijl JM  Veiga P  Walsh S  Wilkinson AJ  Stelling J  Aymerich S 《Science (New York, N.Y.)》2012,335(6072):1099-1103

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Oestrogen and Progesterone Receptor Gene Expression in Canine Oocytes and Cumulus Cells Throughout the Oestrous Cycle     

JSA Gonçalves  CI Vannucchi  FC Braga  FF Paula-Lopes  MP Milazzotto  MEOA Assumpção  JA Visintin 《Reproduction in domestic animals》2009,44(S2):239-242

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Comparison of the Level(s) of DNA Damage Using Comet Assay in Bovine Oocytes Subjected to Selected Vitrification Methods     

EM Stachowiak  K Papis  M Kruszewski  T Iwane&#;ko  T Bart&#;omiejczyk  JA Modli&#;ski 《Reproduction in domestic animals》2009,44(4):653-658

It was suggested that the cryodamage to oocytes' DNA has been responsible for the compromised developmental competence of cryopreserved oocytes. Vitrification of bovine oocytes affected not only cellular components, but also nuclear material. A significant rate of DNA fragmentation was found in bovine frozen or vitrified oocytes analysed by Comet assay regardless of cryopreservation method. Our method of vitrification using droplet system after gentle pre-equilibration treatment is one of the most effective cryopreservation methods employed for bovine oocytes so far, making it possible to develop 30% blastocyst stage embryos. In this study, the extent of DNA damage in bovine oocytes vitrified using three vitrification methods (droplet system, Open Pulled Straw and traditional vitrification in 0.25 ml insemination straws) was compared using Comet assay. Vitrification in droplet system and Open Pull Straws vitrification did not result in detectable cryoinjuries of DNA of bovine oocytes. On the contrary, DNA fragmentation was found in four of 26 oocytes vitrified in 0.25 ml straws (15.4%, p   ≤ 0.05 in comparison with the other vitrification methods).  相似文献   

Acid–Base Changes in Canine Neonates Following Normal Birth or Dystocia     

CF Lúcio  LCG Silva  JA Rodrigues  GAL Veiga  CI Vannucchi 《Reproduction in domestic animals》2009,44(S2):208-210

There are limited data concerning blood gas parameters in neonatal dogs. Knowledge of the normal physiology may facilitate effective therapeutic intervention and potentially reduce neonatal mortality. This study examined acid–base parameters in pups born at normal parturition (n = 27) compared with those born after obstetrical assistance or caesarean operation (n = 13) and those born following oxytocin (OXY) administration for treatment of uterine inertia (n = 11). Pups were subjected to an objective scoring method of neonatal health adapted from use in humans (the Apgar score) at birth and again at 5 and 60 min after birth. Venous blood samples were collected at 5 and 60 min after birth for evaluation of blood gas parameters. At birth, all pups had low Apgar scores and a mixed acidosis. The base excess was lowest for pups delivered after OXY administration. The Apgar score improved for all pups after 5 min of birth and there was an improvement in carbon dioxide tension, base excess and venous blood pH at 1 h, although in all pups a metabolic acidosis persisted. These data provide an important insight into neonatal physiology and the variability of blood gas parameters in pups born at normal and abnormal parturition and provide the basis for clinical decision making following dystocia.  相似文献   

Ligation of the caudal mesenteric artery during resection and anastomosis of the colorectal junction for annular adenocarcinoma in two dogs   总被引：1，自引：1，他引：0  

SK Sarathchandra  JA Lunn  GB Hunt 《Australian veterinary journal》2009,87(9):356-359

An 8-year-old terrier cross and a 10-year-old German Shorthaired Pointer presented to the University Veterinary Centre, Sydney, for investigation of long-standing tenesmus and dyschezia. Both patients had an annular adenocarcinoma at the colorectal junction. Exploratory laparotomy was performed and the affected large intestinal segment was removed by resection and anastomosis. In both dogs, the caudal mesenteric artery was intimately associated with the mass, necessitating its ligation and transection. Postoperatively, there was no evidence of anastomosis breakdown in either case and both animals recovered well from surgery. The dogs were euthanased 8 and 10 months, respectively, after surgery because of clinical signs relating to metastatic disease.  相似文献   

Evaluation of Soy Protein Concentrate and Meat and Bone Meal as a Replacement for Fish Meal in the Diet of Nile Tilapia Fingerlings          下载免费PDF全文

Manoel Joaquim Peres Ribeiro  Rose M. Vidotti  Leandro A. Ferreira  Giovani Sampaio Gonçalves 《Journal of the World Aquaculture Society》2016,47(3):369-375

This experiment evaluated soy protein concentrate (SPC) and meat and bone meal as a replacement for fish meal protein (FP) in the diet of Nile tilapia fingerlings. Five diets were evaluated including a control diet (50.0% crude protein [CP], of which half the protein came from fish meal [FM]), and four diets in which FM was replaced by increasing amounts of SPC (25.5, 50.0, 75.5, and 100.0%) in combination with meat and bone meal. At the conclusion of a 60‐d growth trial, the following parameters were determined: final weight (FW), final length (FL), weight gain (WG), feed intake (FI), apparent feed conversion (AFC), survival (SUR), and production cost. The estimate of the best replacement value was determined by polynomial regression and the linear response plateau. The results showed significant (P < 0.05) improvement for WG and AFC ratio as FM was gradually replaced by SPC. The parameters FL and FI reached their optimum value at a replacement level of 100% SPC. These results show that it is feasible to use a combination of SPC and meat and bone meal in a balanced feed formulation as an alternative protein source in the diets of Nile tilapia fingerlings.  相似文献