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Eckert RE Neuder LE Bell JL Trujillo JC Jones SL 《Veterinary immunology and immunopathology》2007,118(3-4):294-303
The goal of this study was to define the role for p38 mitogen-activated kinase (MAPK) in the signaling mechanism regulating pro-inflammatory cyclooxygenase (COX) gene expression in lipopolysaccharide (LPS)-activated equine leukocytes for the purposes of identifying novel targets for anti-inflammatory therapy in endotoxemic horses. The p38 MAPK has been shown to positively regulate inflammatory gene expression in human leukocytes and can be activated by a variety of stimuli including LPS, TNF-alpha, and IL-1. Activation-associated phosphorylated p38 MAPK has been implicated in the up-regulation of several inflammatory genes, including COX-2 which ultimately results in the production of prostanoids that are responsible for the pathophysiology associated with endotoxemia. Our hypothesis is that activation of p38 MAPK is essential for LPS-induced COX-2 expression in equine peripheral blood leukocytes. We tested our hypothesis by investigating the effects of the specific p38 MAPK inhibitors SB203580 and SB202190 on LPS-induced COX-2 protein expression and PGE(2) production in equine leukocytes. LPS stimulation activated p38 MAPK and increased COX-2 expression in a dose-dependent manner with maximal activation observed after 30min and 4h, respectively, at a concentration of 10 ng/ml LPS. In contrast, LPS stimulation did not affect COX-1 protein expression. Pretreatment with SB203580 or SB202190 significantly inhibited LPS-induced activation-associated p38 MAPK phosphorylation, COX-2 mRNA and protein levels, and PGE(2) production in equine leukocytes. Maximal inhibition of LPS-induced COX-2 protein expression was achieved at a concentration of 10 microM SB203580. We concluded that p38 MAPK is essential for LPS-induced COX-2 expression suggesting that p38 MAPK is a potential target for anti-inflammatory therapy during equine endotoxemia. 相似文献
45.
月季在国际切花市场的销售量一直位居第一,并且是欧洲及世界其他地区公园和庭院的主要观赏植物。但是,蜘蛛螨(二点叶螨)严重影响了陆地和温室栽培月季的质量。这是因为蜘蛛螨能够越冬繁殖且生长发育速度快,并且能快速对大多数广谱杀螨剂产生抗性。人们一直在寻求防治蜘蛛螨的最 相似文献
46.
Samuel Raj Babu Arulmani Vimalan Jayaraj Solomon RobinsonDavid Jebakumar 《Journal of Soils and Sediments》2016,16(3):831-841
Purpose
This study was conducted to determine the existence of soil bacteria in soil by soil microbial fuel cell (SMFC). The main objectives were (1) to differentiate the type of soil which will influence the electricity production, (2) to demonstrate the impact of different volume of soil in the MFC and feeding MFC for long-term electricity production, and (3) to conclude that electricity production is directly proportional to the biofilm formation on the anode surface.Materials and methods
MudWatt kits were purchased from Keego Technologies USA, and 22 identical SMFCs were designed to study the electricity production from agricultural soil (S1) and vermicompost soil (S2). Ten milliliters of bioslurry is fed in SMFC to study the stability of electricity production at different stages. Microbes were isolated and characterized from the surface of the electrode. Biofilm analyses were done by high-content screening (HCS) system using 10 μl of acridine orange (100 μg/ml) at different stages of biofilm, and scanning electron microscopy is applied to confirm the matured biofilm on the surface of the anode.Results and discussion
Application of bioslurry at different stages of electricity production conquers the normal energy recovery of the SMFCs and S2 soil with bioslurry sample produced the highest open circuit voltage (OCV) of 2.8 V (460 days) and S1 soil sample with bioslurry produced 1.7 V (364 days). The difference between SMFCs and MudWatt kits significantly confirms that increasing the volume of soil in the anode part increases the electricity production. The maximum OCV of S1 and S2 in MudWatt kits were 1.5 V (90 days) and 1.8 V (190 days), respectively. Increased volume of soil in our SMFCs produce maximum OCV of 1.8 V (S1 for 173 days) and 2.2 V (S2 for 240 days), and HCS analysis of biofilm at different stages reveals that electricity production is directly proportional to the biofilm formation.Conclusions
Thus, it was concluded that the nature of soil and soil bacterium is important for the electricity production, and S2 soil sample produces maximum electricity than the S1 soil sample. Feeding of SMFCs with bioslurry aids the long-term and stabilized electricity production in both the soil samples.47.
Samuel C. Allen Shibu Jose P. K. R. Nair Barry J. Brecke Vimala D. Nair Donald A. Graetz Craig L. Ramsey 《Biology and Fertility of Soils》2005,41(1):28-37
Information on temporal and spatial patterns of N mineralization is critical in designing tree-crop mixed systems that could maximize N uptake while minimizing N loss. We quantified N mineralization rates in a pecan (Carya illinoensis K. Koch)–cotton (Gossypium hirsutum L.) alley cropping system in northwestern Florida with (non-barrier) and without tree-crop belowground interactions (barrier separating the root systems of pecan and cotton). Monthly rates of mineralization were estimated using buried bag incubations over a 15-month period. In addition, seasonal mineralization rates and cotton lint yield on soils supplied with two sources of N—inorganic fertilizer and organic poultry litter—were assessed. Results indicated that temporal variations in net NH4 and NO3 accumulation and mineralization rates were driven primarily by environmental factors and to a lesser degree by initial soil NH4 and NO3 levels. Mineralization varied by belowground interaction treatment during the initial growing season, when the non-barrier treatment exhibited a higher mineralization rate than the barrier treatment, likely due to reduced nutrient uptake by cotton in the non-barrier or a higher degree of immobilization in the barrier treatment. Mineralization during the second growing season was similar for both treatments. Source of N had no effects on N transformation in the soil. Lint yield reductions were observed in the non-barrier treatment during both years compared to the barrier treatment, likely due to interspecific competition for water. Yield differences between treatments in the second growing season were likely compounded by a diminishing pre-study fallow effect. Source of N was found to have a significant effect on cotton yield, with inorganic fertilizer resulting in 39% higher lint compared to poultry litter in the barrier treatment. 相似文献
48.
Elizabeth A. Thomson Samuel N. Luoma Daniel J. Cain Christopher Johansson 《Water, air, and soil pollution》1980,14(1):215-233
Concentrations of extractable Cu, Zn, Fe and Mn in oxidized estuarine sediment are influenced by the manner and time of storage between collection and extraction. No storage method tested completely preserves the initial chemical and physical characteristics of the sediment. Freezing or (for some extractions) drying are adequate methods of storage if sediments are to be extracted only with acids. However, if a variety of extractants is to be used, extractions should be conducted as soon as practical after collection. 相似文献
49.
A detailed study of the rheological behavior of pectin gels, in the presence of aroma compounds in food concentration is reported. In high methoxylated pectin (HMP) gels, it has not yet been shown if aroma compounds can be responsible for modifications in rheology. Two rheological techniques were used to measure the impact of aroma substances on rheological properties of HMP-based systems. Maximum strain to fracture (sigma F) was compared between flavored and unflavored gels on stress-displacement curve, which was obtained with uniaxial compression until fracture. An oscillatory rheometer was applied to determine the gelation time (Tgel). It appeared that all the aroma compounds studied increased significantly sigma F. It is generally acknowledged that hydrophobic interactions are the main interactions leading to HMP gelation, and the more hydrophobic interactions there are, the higher the sigma F. It is assumed that esters might increase sigma F through the increase of hydrophobic interactions in HMP network. For solvents (ethanol, propylene glycol), a cosolute effect could explain the increase in firmness for HMP-based gels. 相似文献
50.
Silva RD Bueno AL Gallon CW Gomes LF Kaiser S Pavei C Ortega GG Kucharski LC Jahn MP 《Fitoterapia》2011,82(6):818-826
This study analyzed the plasma lipid profile, glucose levels and fat deposits in male rats treated with aqueous extract of gross yerba mate, commercial yerba mate or water. Yerba mate treatment did not change body weight gain and lipid profile. The consumption of gross yerba mate significantly increased blood glucose (6.6 mmol/L) as compared to the water (4.8 mmol/L) and commercial group (5.2 mmol/L) and decreased epididymal and intra-abdominal deposits (10.1 mg/g and 23.7 mg/g of weight) as compared to the water (15.4 mg/g and 36.9 mg/g of weight) and commercial group (12.5 mg/g and 28 mg/g of weight). The results suggest that gross yerba mate reduces fat more efficiently but produces a greater increase in blood glucose when compared to commercial yerba mate and water groups. 相似文献