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51.
Judith A.  Hudson  DVM  PhD  Susan T.  Finn-Bodner  DVM  MS  Joan R.  Coates  DVM  Donald C.  Sorjonen  DVM  MS  Stephen T.  Simpson  DVM  MS  James C.  Wright  DVM  PhD  Jan E.  Steiss  DVM  PhD  Nancy R.  Cox  DVM  PhD  Dana M.  Vaughn  DVM  PhD  Starr C.  Miller  BS  Scott A.  Brown  DVM  PhD  Phillip D.  Garrett  DVM  MS 《Veterinary radiology & ultrasound》1995,36(6):542-547
Doppler ultrasonography of the spinal cord was performed in 34 normal, anesthetized dogs following hemilaminectomy. This study was part of an investigation to evaluate the efficacy of a 21-aminosteroid compound and high dose methylprednisolone for the treatment of spinal cord trauma. Grey-scale images of the canine spinal cord were similar to those described for the spinal cord of people. Doppler waveforms of intraparenchymal spinal arteries exhibited high end diastolic blood flow velocities, indicating low resistance to flow. Doppler values (mean ± SD) for arteries immediately ventrolateral to the central canal were: Peak Systolic Velocity = 5.78 ± 2.5 cm/sec, Minimum Diastolic Velocity = 3.5 ± 1.62 cm/sec, Mean Velocity = 4.45 ± 1.96 cm/sec, Minimum Diastolic Velocity = 3.5 ± 1.62 cm/sec, Mean Velocity = 4.45 ± 1.96 cm/sec, Systolic/Diastolic ratio = 169 ± 0.19, Pulsatility Index = 0.53 ± 0.09, and Resistance Index = 0.4 ± 0.06.  相似文献   
52.
THE SCINTIGRAPHIC EVALUATION OF SOLID PHASE GASTRIC EMPTYING IN NORMAL CATS   总被引:1,自引:0,他引:1  
The gastric emptying half-time of solid food in normal cats was determined using a scintigraphic technique. 99mTc-sulfur colloid was applied to dry food. Sequential images were acquired post prandial, and the gastric emptying half-time was determined for several different acquisition and processing parameters. Good correlation was found between different acquisition and processing methods, some of which made use of edge detection applications, while others used different views, or number of views from which the gastric emptying half-time was calculated. The mean ± standard deviation, (range) and median of the gastric emptying half-time determined using the geometric mean of total counts in hand-drawn regions of interest from the lateral and ventral images were 2.47 × 0.71, (1.42–3.61) and 2.37 hours respectably.  相似文献   
53.
Charles R.  Pugh  DVM  MS  Phillip J.  Johnson  BVSc  MS  Gregory  Crawley  DVM  MS  Susan T.  Finn  DVM  MS 《Veterinary radiology & ultrasound》1994,35(3):183-188
The equine shoulder region is difficult to investigate by conventional imaging modalities. Diagnostic ultrasound has proven valuable for investigation of soft tissue injuries of the human shoulder. In a horse with shoulder lameness, the authors employed diagnostic ultrasound to augment the use of conventional radiography. A defect in the subchondral bone layer of the medial humeral tuberosity, with adjacent bony fragments were clearly identified with ultrasound. Ultrasound was used for initial evaluation of the bicipital tendon and bursa. The technique was subsequently used to follow and evaluate the post-operative condition of the proximal cranial humerus, bicipital tendon and bursa in this patient. The bicipital regions of 2 immature and 3 adult normal horses were also examined with to illustrate the normal ultrasonographic anatomy of the cranial shoulder region.  相似文献   
54.
This report documents congenital stationary night blindness (CSNB) in two non-Appaloosa horse breeds (Thoroughbred and Paso Fino). History of vision impairment since birth, normal ocular structures on ophthalmic examination, and electroretinographic findings were consistent with CSNB. In one horse (Thoroughbred), a 9-year follow-up was carried out. In the Paso Fino, severe vision impairment from birth to approximately 1 year of age in both dim and bright light situations led to humane euthanasia and histopathologic confirmation of the disorder.  相似文献   
55.
OBJECTIVE: To evaluate a method of aerobic bacteriologic culture of epidermal collarette specimens from dogs with superficial pyoderma and compare results with those for aerobic bacteriologic culture of abdominal skin specimens in healthy dogs. DESIGN: Prospective study. ANIMALS: 22 dogs with epidermal collarettes and 24 healthy dogs. PROCEDURE: Dry sterile cotton swabs were rolled across epidermal collarettes or hairless areas of abdominal skin in healthy dogs and submitted for aerobic bacteriologic culture. Hemolytic colonies of gram-positive-staining cocci were tested for catalase production, and if results were positive, a coagulase test was performed. Colonies with coagulase activity were tested for the ability to ferment mannitol. Antimicrobial susceptibility testing was performed on all Staphylococcus spp that were isolated. RESULTS: S. intermedius was isolated from collarettes in 18 of 22 dogs with superficial pyoderma but not from healthy dogs. Estimated sensitivity and specificity of the culture method were 81.8% and 100%, respectively. There were no significant differences in the ability to culture S. intermedius, the number of S. intermedius isolates without resistance to antimicrobials, and the number of S. intermedius isolates resistant to penicillin G when comparing dogs with superficial pyoderma for the first time and dogs with recurrent pyoderma, dogs that did or did not receive concurrent antimicrobials, and dogs with and without underlying allergic disease. CONCLUSIONS AND CLINICAL RELEVANCE: Bacteriologic culture of epidermal collarette specimens was a simple and reliable method for identification of S. intermedius in dogs with superficial pyoderma, regardless of history of pyoderma or current antimicrobial use.  相似文献   
56.
ABSTRACT A multiplex real-time polymerase chain reaction (PCR) assay was developed to simultaneously genotype plants for the I and bc-1(2) alleles, which condition resistance in beans to Bean common mosaic virus and Bean common mosaic necrosis virus. A segregating F(2) population was derived from the cross between pinto bean breeding line P94207-189A (bc-1 bc-1 I I) and Olathe (bc-12 bc-1(2) i i). Real-time PCR assays were developed that were specific for each allele, and a multiplex PCR reaction could unambiguously assign F(2) plants to one of nine genotypes. Remnant F(1) plants were used as a comparative reference sample. PCR results among this sample fit a normal distribution for both real-time PCR assays, and 99% probability distributions were determined for heterozygotes. F(2) plants were genotyped based on results relative to the probability distributions for heterozygotes. F(2) plants also were genotyped for the I and bc-1(2) alleles by performing F(3) family progeny tests for virus resistance. Agreement between the two methods was 100% (198/198) for the bc-1(2) allele, and 92.4% (183/198) for the I allele. Erroneous genotyping was due to recombination between the amplicon and the I allele. Realtime PCR assays provide a robust method for genotyping seedlings and, in some cases, may eliminate the need for progeny testing.  相似文献   
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59.
High-moisture ear corn (HMEC) was treated with specific bacterial inoculants and evaluated for its aerobic stability and utilization for growth by beef steers. Immediately after harvest, HMEC (65% DM) was ensiled in tower silos after being either untreated (control) or treated with the following inoculants: 1) Ecosyl (E); 2) Ecosyl plus Serratia rubidaea (E + SR); and 3) Ecosyl plus Streptococcus thermophilus (E + ST). A portion of HMEC was frozen immediately (-20 degrees C) and subsequently treated with eight bacterial inoculants before ensiling in laboratory silos; the fermented material was then exposed to air for 7 d for assessment of aerobic deterioration. The eight inoculants included the three used in the tower silos and four additional ones: Streptococcus thermophilus, Bacillus subtilis (BS), Serratia rubidaea, and a mixture of Ecosyl + B. subtilis (E + BS). The growth trial was conducted for 112 d with 32 crossbred steers (average BW 296 kg). A digestion trial was conducted, according to a 4 x 4 Latin square design, using an additional four steers (average BW 367 kg). In both trials, steers were fed the same four diets containing inoculated (E, E + SR, and E + ST) or control HMEC. Upon exposure to air, Ecosyl-treated ensiled HMEC had the least increase in pH compared with other single inoculants; all inoculant treatments lessened (P less than .05) the increase in sample temperature compared with control. During aerobic exposure, treatment of HMEC with BS seemed to reduce the disappearance of water-soluble carbohydrates, whereas Ecosyl seemed to reduce lactic acid. Despite evidence of improved aerobic stability with Ecosyl and BS, inoculation of HMEC did not (P greater than .10) improve BW gain or feed efficiency; however, all inoculants reduced (P less than .05) digestibility of ADF.  相似文献   
60.
A 2-year-old spayed female mixed-breed dog was referred with an 8-month history of recurrent bilateral anterior uveitis and hyphema secondary to infection with Brucella canis. Treatment with doxycycline PO and atropine and prednisone acetate topically prior to referral had failed to resolve the ocular inflammation. Successful control of ocular inflammation was achieved after initiation of treatment with gentamicin SC and ciprofloxacin and doxycycline PO. The finite indirect fluorescent antibody (IFA) titer was reduced by half, and results of an agar gel immunodiffusion (AGID) test became negative after rifampin was added to the treatment protocol. Treatment with gentamicin was eventually discontinued because of evidence of renal toxicosis and cutaneous reactions to repeated injections. Thirty-five months after initial examination of the dog, results of the AGID test were still negative, the finite IFA titer was stable, and ocular inflammation had resolved, suggesting that the ocular infection may have been eliminated. To our knowledge, this represents the first report of the successful treatment of ocular inflammation caused by B canis infection in a dog.  相似文献   
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