An outbreak of tuberculosis by Mycobacterium bovis in coatis (Nasua nasua)     

Murakami PS  Monego F  Ho JL  Gibson A  Vilani RG  Soresini GC  Brockelt SR  Biesdorf SM  Fuverki RB  Nakatani SM  Riediger IN  Grazziotin AL  do Santos AP  de Barros Filho IR  Biondo AW 《Journal of zoo and wildlife medicine》2012,43(2):338-341

Mycobacterium tuberculosis complex, which includes Mycobacterium bovis, infrequently causes severe or lethal disease in captive wildlife populations. A dead coati from a wildlife triage center showing pulmonary lesions compatible with tuberculosis had raised suspicion of a potential disease caused by mycobacteria species and was further investigated. Four native coatis (Nasua nasua) with suspected mycobacterial infection were sedated, and bronchoalveolar lavages and tuberculin skin tests (TSTs) were performed. All animals tested positive upon TST. Mycobacterial culturing, Ziehl-Neelsen staining, and genetic testing were performed on postmortem samples and the etiologic agent was identified as M. bovis. Molecular genetic identification using a polymerase chain reaction panel was crucial to achieving a definitive diagnosis.  相似文献   

Characterization of kaolinite in the hardsetting clay fraction using atomic force microscopy,X-ray diffraction,and the Rietveld method     

Luis Valério Prandel  Nívea Maria Piccolomini Dias  Sérgio da Costa Saab  André Maurício Brinatti  Neyde Fabíola Balarezo Giarola  Luiz Fernando Pires 《Journal of Soils and Sediments》2017,17(8):2144-2155

  相似文献   

Effect of pearl millet silage ammoniated with urea on lamb production and metabolic performance          下载免费PDF全文

G. G. P. Carvalho  P. M. D. Freitas  E. M. Santos  G. G. L. Araújo  J. S. Oliveira  A. J. V. Pires  C. M. A. Maranhão  T. C. G. C. Rodrigues  J. E. Freitas Júnior  L. M. A. Rufino  C. S. Rodrigues  L. C. Leite  M. L. G. M. L. Araujo 《Grass and Forage Science》2018,73(3):685-693

The objective of this study was to determine the effect of diets containing pearl millet silage ammoniated with urea on the intake, digestibility, production performance, and nitrogen metabolism of lambs. Thirty‐two uncastrated mixed‐breed lambs at 4 to 5 months of age, with an average initial body weight of 17.39 ± 2.16 kg, were distributed into four treatments in a randomized block experimental design with eight replicates. Experimental diets consisted of pearl millet silage ammoniated with urea during ensiling at the levels of 0, 20, 40, and 60 g/kg dry matter (DM). Increasing urea levels in the pearl millet silage led to a linear decrease (p < .05) in the intakes of DM, organic matter, and total digestible nutrients by the lambs. Total and average daily weight gains decreased linearly (p < .05) with the addition of urea to the silage. Intake and digestibility of crude protein, nitrogen intake, urine urea nitrogen, plasma urea nitrogen, microbial synthesis, and microbial efficiency had a quadratic response (p < .05) to the urea levels in the silage. Pearl millet silage ammoniated with urea reduces dry‐matter intake and daily weight gain in lambs. Urea is not recommended for use in the ensiling of pearl millet.  相似文献   

Straelensiosis in a dog in Portugal     

Seixas F  Travassos P  Pinto M  Pires I  Pires MA 《The Veterinary record》2003,153(5):156

  相似文献   

Vascularization and Production of Mitogenic Factor(S) in Domestic Cat (Felis catus) Testes: Preliminary Results     

A. Alexandre- Pires  G. Ferreira-Dias  L. Mateus    L. Mendes- Jorge  J. M. Cifuentes    N. Alemañ 《Anatomia, histologia, embryologia》2005,34(S1):2-2

Some male seasonal breeders are known to undergo testicular growth and regression throughout the year. The periodic and fast physiological development and regression processes, with simultaneous fast changes in blood flow, appears to be stimulated by angiogenic substances and inhibited by anti-angiogenic factors. The objective of this study was to evaluate the effect of season on microvascularization and production of angiogenic factors by testicular tissue, using the male cat as a model. Monthly, testes were collected post mortem from domestic stray cats, for histology and for tissue culture. Testis explants were cultured for 18h and conditioned media were tested for their ability to stimulate mitogenesis of bovine aortic endothelial cells (BAEC). Vascular endothelial growth factor (VEGF) was used as a positive control. Proliferative response of BAEC to samples was evaluated by determining the number of cells in each well using a Neubauer chamber. Vascular density was assessed by a computerized image analysis ('Scion Image, NIH, USA') based on the total histological area occupied by the vascular lumen. Percentage data subjected to arcsine transformation were analysed by one-way ANOVA and post-comparison tests. There was a significant increase in microvascular areas in November ( P  < 0.001), when compared to the remaining months (LSD test). All cat testicular tissue showed the capability to increase BAEC proliferation, when compared to negative controls, throughout the study. However, there was an increase in BAEC mitogenesis in February ( P  < 0.05) and November ( P  < 0.001), when compared to the other months. Our data show that cat testicular tissue from different periods of the year stimulates angiogenic factor(s). Furthermore, changes in angiogenesis may play a role on vascular growth and regression of the testes during the breeding and non-breeding season in the male cat.  相似文献   

Occurrence of Raoiella indica Hirst (Acari,Tenuipalpidae) in the Southeast region of the state of Pará, Brazil     

da Cruz  Wilton Pires  Souza  Magno Chagas  Lacerda  José Dantas Araújo  Souza  Áurea Izabel Aguiar Fonseca e  Silva  Priscilla Andrade  dos Santos  Elenilza Cruz 《植物病害和植物保护杂志》2023,130(1):199-204

Journal of Plant Diseases and Protection - The red palm mite Raoiella indica Hirst attacks coconut, banana and açai trees. In 2007, the mite was recorded for the first time in South America....  相似文献   

Tumour‐associated macrophages are associated with vascular endothelial growth factor expression in canine mammary tumours          下载免费PDF全文

T. P. Raposo  I. Pires  M. I. Carvalho  J. Prada  D. J. Argyle  F. L. Queiroga 《Veterinary and comparative oncology》2015,13(4):464-474

Tumour‐associated macrophages (TAMs) have been implicated in carcinogenesis including an important role in angiogenesis. In this study, we describe the relationship between TAMs and angiogenesis in canine mammary tumours (CMT). Formalin‐fixed paraffin‐embedded CMT samples [(n = 128: malignant (n = 97) and benign (n = 31)] were submitted to immunohistochemical staining to detect MAC387, vascular endothelial growth factor VEGF and CD31 expression. A statistical analysis was carried out to assess possible associations with clinicopathological variables and biological markers of tumour angiogenesis. TAMs, detected by MAC387 expression, were significantly associated with malignant CMT (P < 0.001) and VEGF positive tumours (P = 0.002) and also associated with VEGF expression within malignant CMT (P = 0.043). Associations with clinicopathological variables were found between TAMs and the presence of infiltrative growth (P = 0.031), low tubule formation (P = 0.040) and lymph node metastasis (P = 0.016). The results support the hypothesis that TAMs influence angiogenesis in CMT suggesting TAMs may represent a therapeutic target in this disease.  相似文献   

Field study on nematode resistance in Nelore-breed cattle     

Bricarello PA  Zaros LG  Coutinho LL  Rocha RA  Kooyman FN  De Vries E  Gonçalves JR  Lima LG  Pires AV  Amarante AF 《Veterinary parasitology》2007,148(3-4):272-278

The present study evaluated Nelore cattle with different degrees of resistance to natural infections by gastrointestinal nematodes. One hundred weaned male cattle, 11-12 months of age, were kept on the same pasture and evaluated from October 2003 to February 2004. Faecal and blood samples were collected for parasitological, haematological and immunological tests. In February 2004, the 10 most resistant and the 10 most susceptible animals were selected based on individual means of nematode faecal egg counts (FEC). Such animals were slaughtered for worm burden determination and nematode species identification. The repeatability estimates for FEC (+/-S.D.), log-transformed FEC and packed-cell volume (PCV) in all animals were 0.3 (+/-0.05), 0.26 (+/-0.04) and 0.42 (+/-0.05), respectively. The resistant group showed lower FEC and worm burdens than the susceptible group (P<0.05). There were no significant differences between groups regarding mean body weight, weight gain, PCV and total serum protein values (P>0.05). The resistant group showed higher total serum IgE levels (P<0.05) and higher mean eosinophil blood counts. However, the latter was statistically significant only 42 days after the beginning of the study. Nematodes Cooperia punctata and Haemonchus placei were predominant and the correlation between Cooperia and Haemonchus burdens was 0.64 (P<0.05), which indicated that animals presenting increased numbers of one of those genera probably had increased numbers of the other. The current study provides further evidence of IgE active role in nematode immunity and suggests that total serum IgE level might serve as an additional marker to select Nelore cattle that are responsive to H. placei and C. punctata infections.  相似文献   

Commercial Bacillus probiotic supplementation of rainbow trout (Oncorhynchys mykiss) and brown trout (Salmo trutta): growth,immune responses and intestinal morphology          下载免费PDF全文

Maria Amélia Ramos  José F M Gonçalves  Benjamín Costas  Sónia Batista  Rebecca Lochmann  Maria A Pires  Paulo Rema  Rodrigo O A Ozório 《Aquaculture Research》2017,48(5):2538-2549

Probiotic administration is associated with the enhancement of host resistance to environmental and nutritional stressors, improving survival and growth rates. This study was carried out to evaluate the effects of dietary supplementation of Bacillus subtilis and Bacillus cereus toyoi on growth performance, innate immune responses and gut morphology of two trout species feeding a commercial diet high in soybean meal. A commercial probiotic (4.2 × 10⁹ CFU g^?1 of additive) was supplemented to the experimental diets at 0% (control), 0.03% (P₁; 6 × 10³ CFU g^?1 of diet) or 0.06% (P₂; 1.5 × 10⁶ CFU g^?1 of diet) and fed to brown trout (Salmo trutta) and rainbow trout (Oncorhynchus mykiss) for 9 and 20 weeks respectively. Rainbow trout showed significantly better growth performance than brown trout, regardless of the dietary treatment. No effect of dietary probiotic supplementation was detected on growth performance, body composition or innate immune parameters (plasma lysozyme, alternative complement and peroxidase activities). In both species, after 9 weeks, intestinal lamina propria and submucosa were widened, with increased presence of inflammatory cells, significantly higher in groups fed probiotics. This inflammatory process, with villi and enterocytes noticeably damaged compared with the control group, was more pronounced in brown trout. Under the current trial conditions, the B. subtilis + B. cereus toyoi had no positive impact in either trout species, on the contrary a harmful effect was observed.  相似文献   

Phytotoxic effect of culture filtrate from Fusarium subglutinans the causal agent of fusariose of pineapple (Ananas comosus (L.) Merr     

Orlando Borrás Hidalgo  Aristoteles Pires de Matos  Renato Santos Cabral  Raúl Tapia Tussel  Mayda Arzola  Ramón Santos  María Cristina Pérez 《Euphytica》1998,104(2):73-77

Two pineapple varieties differing in resistance to fusariose were examined for the phytotoxic effect of Fusarium subglutinans culture filtrate. The cultivars were Perolera (more resistant to pathovars of Fusarium subglutinans) and Smooth Cayenne (more susceptible). The phytotoxic effect of culture filtrate was assessed in tissue culture pineapple plantlets (by electrolyte leakage and placing the culture filtrate on wound leaf segments) and callus (inhibition of growth). Smooth Cayenne proved to be the most sensitive cultivar in each test, whereas Perolera showed resistance to the culture filtrate and its callus grew in the presence of high concentrations of culture filtrate that were completely toxic to Smooth Cayenne. These results show that plants can display cellular resistance to the culture filtrate. This revised version was published online in August 2006 with corrections to the Cover Date.  相似文献