An acute severe outbreak of infectious bovine rhinotracheitis: clinical, epidemiological, microbiological and pathological aspects.     

A Wiseman  P M Msolla  I E Selman  E M Allan  H J Cornwell  H M Pirie  W S Imray 《The Veterinary record》1978,103(18):391-397

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Ovine Bronchopulmonary Globule Leyukocytes     

G. S. Mahmoud  H. M. Pirie 《Anatomia, histologia, embryologia》1982,11(3):205-212

This study describes globule leukocytes in the respiratory system of parasitized and parasite free sheep. Globule leukocytes are charcterized morphologically and cytochemically and compared with those described by other authors. the possible origin and function of globular leuklocytes is discussed.  相似文献   

Chronological Appearance of Apoptosis in Bovine Embryos Reconstructed by Somatic Cell Nuclear Transfer from Quiescent Granulosa Cells     

JO Gjørret  J Wengle  P Maddox-Hyttel  WA King 《Reproduction in domestic animals》2005,40(3):210-216

Efficiency of cloning has remained low and in spite of attempts to improve this technology, many reconstructed embryos do not implant or are lost during early pregnancy. Chromosomal aberrations, deviant gene expression patterns and abnormal regulation of cell death may be involved in this increased early embryonic loss. Here, we investigate the chronological onset of both apoptotic changes in nuclear morphology and DNA degradation [detected by transferase-mediated dUTP nick-end labelling (TUNEL) reaction] in bovine two-cell- to blastocyst-stage embryos. Such embryos were generated either by reconstruction with nuclear transfer from quiescent granulosa cells or by regular in vitro embryo production. Nuclear condensation was observed from the two-cell stage and TUNEL labelling was observed from the six-cell stage in reconstructed embryos, whereas nuclear condensation was evident from the eight-cell stage and TUNEL labelling from the 13-cell stage in embryos derived in vitro. Furthermore, reconstructed embryos displayed elevated ratios of embryos containing apoptotic nuclei at pre-compaction stages and higher indices of apoptotic nuclei in morula and blastocyst stages when compared with in vitro-produced embryos.  相似文献   

Reliability of manual measurements of corneal thickness obtained from healthy canine eyes using spectral-domain optical coherence tomography (SD-OCT)     

Anthony F. Alario  Christopher G. Pirie 《Canadian journal of veterinary research》2014,78(3):221-225

The purpose of this study was to manually measure corneal thickness in canine eyes using a spectral-domain optical coherence tomography (SD-OCT) device and to assess intra- and inter-observer reliability of this technique. Twenty healthy dogs with a mean age of 4.7 y were examined. A 6-mm corneal pachymetry protocol was carried out by 1 operator using 1 SD-OCT device in both eyes of each animal. Measurements were obtained manually and in duplicate by 2 independent investigators (> 24 h apart), using the built-in caliper function. Measurements included epithelial thickness (ET), non-epithelial thickness (NET), and central corneal thickness (CCT). The overall mean ET, NET, and CCT for all eyes examined were 72.3 ± 4.6 μm, 538.9 ± 42.5 μm, and 611.2 ± 40.3 μm, respectively. There was no significant difference in ET, NET, or CCT based on the eye examined [oculus dexter (OD) versus oculus sinister (OS)], age, or gender of the animal. There was no significant difference in replicate measurements of ET, NET, or CCT done by the same operator, although a small but significant difference was noted between operators for ET measurements only. The mean difference in ET between operators was 0.6 μm (P = 0.03). The coefficient of variation ranged from 0.5% to 9.27% and intraclass correlation coefficient ranged from 0.35 to 0.97. Based on these results, manual measurements of corneal thickness in canine eyes using a portable SD-OCT device provided ET, NET, and CCT measurements with clinically acceptable intra- and inter-observer reliability.  相似文献   

The effects of exposure to water-soluble fractions of crude oil on selected histochemical parameters of the liver of Atlantic croaker, Micropogon undulatus L.     

JO ANN EURELL  W. E. HAENSLY 《Journal of fish diseases》1981,4(3):187-194

Abstract. Micropogon undulatus L. were sampled from Galveston Bay, Texas, and transported to the research laboratory. Groups of five fish were exposed to 5 and 10% dilutions of the water soluble fraction (WSF) of southern Louisiana crude oil for 1, 3, 7, 14 and 21 days. Selected histochemical parameters of liver sections from control and WSF-exposed fish were evaluated. Glycogen was reduced in amount while glucose-6-phosphate dehydrogenase, lipid and cholesterol were increased in liver sections of WSF-exposed fish. These observations support a hypothesis that exposure to petroleum hydrocarbons increases mixed-function oxidase activity. The alterations were more obvious in sections from fish exposed to the higher level of the WSF. The histochemical parameters appeared most altered in hepatocytes near afferent hepatic blood vessels. These hepatocytes were the first cells of the liver to contact the pollutants.  相似文献   

Inhaled ciclesonide is efficacious and well tolerated in the treatment of severe equine asthma in a large prospective European clinical trial     

Robert Scott Pirie  Hanns-Walter Mueller  Odilo Engel  Balazs Albrecht  Marcella von Salis-Soglio 《Equine veterinary journal》2021,53(6):1094-1104

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The anti-inflammatory effects of IV administered clenbuterol in horses with recurrent airway obstruction     

Laan TT  Bull S  Pirie RS  Fink-Gremmels J 《Veterinary journal (London, England : 1997)》2006,171(3):429-437

Cyclic AMP elevating agents have been shown to exhibit anti-inflammatory properties in addition to functions such as bronchodilation. The aim of this study was to investigate this dual action of clenbuterol (CB; Ventipulmin) on horses affected with recurrent airway obstruction (RAO). Seven RAO susceptible horses received inhalation challenges with aerosolised lipopolysaccharide (LPS), hay dust suspension (HDS) and Aspergillus fumigatus antigen (AF) with and without prior treatment with intravenous CB. Data showed that CB exerted significant beneficial effects on lung function, total cell count (TCC) and bronchoalveolar lavage neutrophil influx. In addition, CB significantly decreased the expression of several pro-inflammatory cytokines and chemokines in the alveolar macrophages of RAO-susceptible horses after challenge with LPS and HDS, and increased the expression of interleukin-6, known to act as a pro-and anti-inflammatory cytokine, following different challenges. This anti-inflammatory activity of CB is of additive value to its currently recognised use in equine RAO.  相似文献   

Canine conjunctival hemangioma and hemangiosarcoma: a retrospective evaluation of 108 cases (1989-2004)     

Pirie CG  Knollinger AM  Thomas CB  Dubielzig RR 《Veterinary ophthalmology》2006,9(4):215-226

Canine conjunctival tumors of vascular endothelial origin are common, although under-reported. The purpose of this study was to evaluate the epidemiology of and potential risk factors for these tumors. This study evaluated 108 cases (70 hemangiomas, 38 hemangiosarcomas) from 8300 canine submissions between 1989 and 2004. Signalment, location, pigmentation, size, duration, diagnosis, margins, ancillary therapy, and geographic location were recorded. Follow-up information was available for 49 cases. Each case was matched with two unaffected controls and compared using logistic regression analysis. Average age upon presentation was 8.6 years; there was no sex predilection. Risk of conjunctival tumors was statistically different among breed groups (P = 0.0010), demonstrating a propensity to occur in groups likely to have increased outdoor activity. Primary involvement occurred within nonpigmented epithelium along the leading edge of the nictitating membrane (41/108) and temporal bulbar conjunctiva (33/108). The etiology remains unknown; however, the strong site predilection, involvement of nonpigmented epithelium, and development within specific breed classes strongly suggest ultraviolet (UV) light as a significant risk factor. In a full-logistic model including breed, gender, age, and UV exposure, UV was not a statistically significant variable (P = 0.1215). In a reduced-model including UV only, significance was approached (P = 0.0696) and posthoc contrast demonstrated a significant linear trend with increasing UV exposure (P = 0.0147). In separate analysis of risks associated with hemangiosarcoma, compared with hemangioma, breed was not significant while increasing UV exposure was significant (P = 0.0381). Early surgical therapy is recommended and may be curative; however, recurrence is possible and more likely with hemangiosarcomas (11/20).  相似文献   

Feline conjunctival hemangioma and hemangiosarcoma: a retrospective evaluation of eight cases (1993-2004)     

Pirie CG  Dubielzig RR 《Veterinary ophthalmology》2006,9(4):227-231

The purpose of this retrospective study was to evaluate feline primary conjunctival vascular tumors of endothelial origin. Eight cases (six hemangiomas, two hemangiosarcomas) from a collection of 3460 feline submissions between 1993 and 2004 were evaluated using routine hematoxylin and eosin (H&E). Signalment, location, size, duration, epithelial pigmentation, margins, adjuvant therapy, outdoor activity, and geographic location, comparing ultraviolet (UV) radiation levels by state, were recorded. Follow-up information was available for five cases. In this study, the average age was 10.6 years, with neutered males over-represented. The Domestic Short-haired cat was most commonly affected. The most common anatomic site was the nictitating membrane, with the left eye preferentially affected. The average size and duration, prior to presentation, was 7.5 mm and 4.4 months, respectively. Seven of eight cases were devoid of epithelial pigmentation in nonaffected areas and the majority of cases were from states with high annual UV-light exposure. Only cases of hemangiosarcoma underwent surgical re-excisions following incomplete excision; however, no further recurrences were reported. No cases evaluated had evidence of metastatic disease at the time of excision. Surgical excision alone may be curative. However, recurrence is possible. These tumors demonstrate similar predilection sites and involvement of nonpigmented epithelium, as is true in canine cases, which may relate to risk factors as well.  相似文献   

Neurodegeneration in equine grass sickness is not attributable to niacin deficiency          下载免费PDF全文

B. C. McGorum  R. C. Jago  E. Cillan‐Garcia  R. S. Pirie  J. A. Keen  R. J. M. Reardon  P. Y. Saffu  N. J. Miller 《Equine veterinary journal》2017,49(4):445-447

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