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We carried out a trawl-depletion experiment for benthic fauna in an area closed to commercial trawling. A 12-fathom prawn (shrimp) trawl net having a swept path of about 18 m was towed 13 times along each of six tracks. Each track was 2.7 km long and known from previous surveys to contain several relatively dense patches of megabenthos. The depletion rate was assumed to vary between trawls on a given track, and was modelled by a beta distribution defined by a mean rate and a dispersion parameter. Maximum likelihood analysis of the sequence of catches on each track suggests that a single trawl removes a small, but non-negligible, fraction of the benthos. The combined effect of 13 trawls on each track, assuming the trawls were aligned, was to remove a substantial proportion of the initial biomass. Gastropods suffered the greatest impact (an estimated 95% removed, on average). Ascidians, sponges, echinoids, crustaceans and gorgonians were depleted by an estimated 74–86%, and all other taxonomic groups except algae (27% removed) were reduced by at least 54%. Using differential global positioning systems and careful navigation, we aimed to achieve a high degree of overlap between successive trawls. Nonetheless, records show that the trawler deviated from this path and it is likely that the net did not sweep exactly the same path during the 13 trawls on each track. A simulation study was conducted to examine the effect on parameter estimates of ignoring the probable non-alignment of trawls. There was little bias in the estimate of the dispersion parameter. With the level of dispersion found on these tracks, the estimated mean depletion rates would, however, have been biased (upwards when the true mean depletion rate was below 15%, downwards when the true mean depletion rate was above 15%). This means that most mean depletion rates estimated from the catch data are probably biased by 2–3%. The depletion rates estimated from this study may be acceptable in areas that are trawled infrequently or sparsely. However, the cumulative effect of frequent trawls in an area of high intensity trawling is likely to be substantial both in terms of organisms directly affected by trawling and indirectly due to attracting scavengers and removing refuge habitat for fish and other mobile organisms.  相似文献   
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Background

South America is one of the most species diverse continents in the world. Within South America diversity is not distributed evenly at both local and continental scales and this has led to the recognition of various areas with unique species assemblages. Several schemes currently exist which divide the continental-level diversity into large species assemblages referred to as biomes. Here we review five currently available biome maps for South America, including the WWF Ecoregions, the Americas basemap, the Land Cover Map of South America, Morrone's Biogeographic regions of Latin America, and the Ecological Systems Map. The comparison is performed through a case study on the Seasonally Dry Tropical Forest (SDTF) biome using herbarium data of habitat specialist species.

Results

Current biome maps of South America perform poorly in depicting SDTF distribution. The poor performance of the maps can be attributed to two main factors: (1) poor spatial resolution, and (2) poor biome delimitation. Poor spatial resolution strongly limits the use of some of the maps in GIS applications, especially for areas with heterogeneous landscape such as the Andes. Whilst the Land Cover Map did not suffer from poor spatial resolution, it showed poor delimitation of biomes. The results highlight that delimiting structurally heterogeneous vegetation is difficult based on remote sensed data alone. A new refined working map of South American SDTF biome is proposed, derived using the Biome Distribution Modelling (BDM) approach where georeferenced herbarium data is used in conjunction with bioclimatic data.

Conclusions

Georeferenced specimen data play potentially an important role in biome mapping. Our study shows that herbarium data could be used as a way of ground-truthing biome maps in silico. The results also illustrate that herbarium data can be used to model vegetation maps through predictive modelling. The BDM approach is a promising new method in biome mapping, and could be particularly useful for mapping poorly known, fragmented, or degraded vegetation. We wish to highlight that biome delimitation is not an exact science, and that transparency is needed on how biomes are used as study units in macroevolutionary and ecological research.  相似文献   
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CASE HISTORY: A feral cat captured in the Manawatu region of New Zealand was treated for worms and fleas, and kept confined in a metabolic cage. It showed good appetite and weight gain but had intermittent watery, yellow diarrhoea.

CLINICAL FINDINGS: Clinical examination under sedation was unremarkable and routine blood tests showed no significant abnormalities. The cat was negative for feline immunodeficiency virus (FIV) and feline leukaemia virus (FeLV). Different canned cat foods did not alter the course of the diarrhoea, and the cat was euthanised 6 months after capture. At necropsy, two sections of adult Spirometra tapeworms were found in the jejunum and typical Spirometra eggs were found in colonic contents. Molecular identification of the parasite was undertaken, using the cytochrome- c oxidase subunit-1 gene (cox1) sequence.

DIAGNOSIS: Chronic intermittent diarrhoea associated with Spirometra erinacei / S. erinaceieuropaei infection.

CLINICAL RELEVANCE: Spirometra has not been reported in New Zealand before but has been associated with gastrointestinal disease in cats in other parts of the world. It requires speciestargeted treatment to be eliminated effectively, and is zoonotic. Diagnosis could be diffi cult for clinicians who are not familiar with the parasite and its life cycle.  相似文献   
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The aim of this study was to evaluate the influence that different protocols of urethral catheterization after pharmacological induction (Ur.Ca.P.I.) may have on the semen quality of the domestic cat. The study has been divided into two experiments: one in which different dosages of medetomidine administrated are evaluated and the second one in which the timing of the catheterization after pharmacological induction is tested. In the first experiment, 18 cats were sedated with the recommended dosage of medetomidine (130 μg/kg i.m.) while the other 18 were sedated with a lower dose of the same drug (50 μg/kg i.m.). In the second experiment, three groups were implemented, each containing 25 subjects. In group 1, the semen collection was performed immediately once the pharmacological effect of the drug was reached; in group 2, the semen collection was performed three times every 5 min after the pharmacological effect was reached; finally, in group 3, Ur.Ca.P.I. was performed 20 min after the pharmacological effect was reached. All the different protocols permitted sperm collection, nevertheless the first experiment showed a better quality in terms of volume, concentration, total number of spermatozoa (p < 0.01) and quality of the movement (motility p < 0.05 and forward progressive motility p < 0.01), using a high medetomidine dosage rather than 50 μg/kg i.m. In the second experiment, forward motility was statistically higher (p < 0.01) in the first group and total volume was higher (p < 0.01) in the second and third group, while other parameters were statistically not different. Results suggest that a single catheterization immediately after the onset of the pharmacological effect leads to a good‐quality semen with the lowest possibility of damaging the urethra and that a sedation with 130 μg/kg of medetomidine leads to a better quality sperm collection than 50 μg/kg does.  相似文献   
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