侵染菜豆的番茄不孕病毒的研究简报   总被引：1，自引：0，他引：1  

马德芳  邱并生  田波  胡伟贞  张作芳  张成良  宋淑敏 《植物保护学报》1983,10(3):215-216

从北京田间表现系统花叶的菜豆上分离到一个病毒,繁殖在普通烟上,将接种后3～4周发病叶片,加0.5M柠檬酸缓冲液(pH6.5),内含0.5%巯基乙醇,捣碎,经氯仿乳化,低速离心后加10%PEG(M.W6000)和4%NaCl沉淀过夜,低速离心收集沉  相似文献   

The effects of the supplementation of multi‐strain probiotics on intestinal microbiota,metabolites and inflammation of young SPF chickens challenged with Salmonella enterica subsp. enterica     

Chi Huan Chang  Po Yun Teng  Tzu Tai Lee  Bi Yu 《Animal Science Journal》2019,90(6):737-746

This study assessed the effect of probiotics on cecal microbiota, cecal short‐chain fatty acids (SCFAs), and the gene expression of cytokines in young specific‐pathogen‐free (SPF) chickens infected with S. enterica subsp. enterica. One‐day‐old SPF chickens (n = 105) were randomly assigned to one of the three treatment groups: control (Cont) group, Salmonella‐infected (Sal) group, and a Salmonella‐infected group treated with multi‐strain probiotics (ProSal group). All chickens except those in the Cont group were challenged orally with 1 × 10⁸ cfu/ml of Salmonella 4 days after hatching. Chickens in the Sal group exhibited more abundance of Proteobacteria than those in the Cont and ProSal groups. At the genus level, chickens in ProSal group exhibited increased numbers of Lactobacillus and Oscillospira compared with those in the other groups. Chickens in the ProSal group exhibited a significant increase of cecal SCFAs compared with chickens in the Sal group. Chickens in the ProSal group exhibited increased gene expression of anti‐inflammatory cytokines, IL‐10 and TGF‐β4, and decreased expression of the proinflammatory cytokine, IFN‐γ, in the cecal tonsil compared with those in the Sal group. The results of this study indicated that the administration of probiotics can modulate microbiota, SCFAs, and immunomodulatory activity in SPF chickens.  相似文献   

Antibacterial Efficacy of Several Surgical Hand Preparation Products Used by Veterinary Students          下载免费PDF全文

Po‐Yen Chou  Aimie J. Doyle  Shiori Arai  Pierre J. Burke  Trina R. Bailey 《Veterinary surgery : VS》2016,45(4):515-522

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Detection of Zoonotic Protozoa Toxoplasma gondii and Sarcocystis suihominis in Wild Boars from Spain          下载免费PDF全文

R. Calero‐Bernal  J. E. Pérez‐Martín  D. Reina  F. J. Serrano  E. Frontera  I. Fuentes  J. P. Dubey 《Zoonoses and public health》2016,63(5):346-350

Food safety regulations require the control of the presence of protozoa in meats destined for human consumption. Wild boar (Sus scrofa) meat may constitute a source of zoonoses. A 23.8% (688/2881) seroprevalence of anti‐Toxoplasma gondii antibodies and 72.2% (662/910) Sarcocystis sarcocysts prevalence were detected among wild boars hunted in Southwestern areas of Spain. Identity of Sarcocystis spp. was performed by RFLP‐PCR and sequencing, detecting S. miescheriana (7/8) and the zoonotic S. suihominis (1/8). Risk assessment studies of these coccidian in meats destined to human consumption are needed.  相似文献   

感染花叶病毒的马铃薯块茎中含氮化合物的初步分析     

田波  虞明霞  崔文华  赵淑珍 《植物保护学报》1963,2(4):417-422

测定了自然感染花叶病毒(x y)的马铃薯男爵品种在不同地理(北京和克山)、季节(春播和秋播)和人工控制的土温(25°及15℃)三类条件下形成的退化及未退化块茎中的总氮(TN)、非蛋白质氮(NPN)、蛋白质氮(PN)、氨态氮、醯胺氮(AN)和氨基氮(NH_2—N)的变化。各种条件下形成的退化及未退化块茎中,TN 都没有显著差别,而 NPN 和PN 却有明显变化,退化块茎中 NPN 高于 PN.(NPN)/(PN)值为1.2—1.6;而未退化块茎中 PN 高于 NPN,(NPN)/(PN)值为0.6—1.0。此比值的大小与块茎退化程度成正相关。在进一步的测定中证明 NPN 的升高,主要是 NH_2—N 和 AN 的增加。  相似文献   

Genomic and phylogenetic characterization of Shewanella xiamenensis isolated from giant grouper (Epinephelus lanceolatus) in Taiwan     

Yao‐Ting Huang  Jan‐Fang Cheng  Zong‐Yen Wu  Kwong‐Chung Tung  Ying‐Ju Chen  Yu‐Kai Hong  Shi‐Yu Chen  Po‐Yu Liu 《Zoonoses and public health》2019,66(6):679-685

Shewanella xiamenensis is an emerging pathogen causing intra‐abdominal infection and intestinal colonization. Epidemiologic clues suggest its role as a potential food‐borne zoonotic agent. To date, four genome sequences of S. xiamenensis have been made publicly available. All of them were isolated from water samples. In this study, we characterized the genome of a S. xiamenensis strain isolated from a giant grouper in Taiwan. The genome of S. xiamenensis ZYW1 is 4,827,717 bp in length and encodes 4,239 open reading frames. Its genomic sequence shares high homology with other S. xiamenensis strains. bla_OXA‐416 was identified. This is the first detection of S. xiamenensis in Taiwan. These genomic data and analyses contribute to our understanding of S. xiamenensis and may help to elucidate disease‐causing mechanisms in future studies.  相似文献   

Stk2, a Mitogen-Activated Protein Kinase from Setosphaeria turcica,Specifically Complements the Functions of the Fus3 and Kss1 of Saccharomyces cerevisiae in Filamentation,Invasive Growth,and Mating Behavior     

Shou-qin GU  Yang YANG  Po LI  Chang-zhi ZHANG  Yu FAN  Xiao-yu ZHANG  Lan TIAN  Zhi-min HAO  Zhi-yan CAO  Xiao-dong GONG  Yong-shan FAN  Jian-min HAN  Jin-gao DONG 《农业科学学报》2013,12(12):2209-2216

Setosphaeria turcica, an essential phytopathogenic fungus, is the primary cause of serious yield losses in corn; however, its pathogenic mechanism is poorly understood. We cloned STK2, a newly discovered mitogen-activated protein kinase gene with a deduced amino acid sequence that is 96% identical to MAK2 from Phaeosphaeria nodorum, 56% identical to KSS1 and 57% identical to FUS3 from Saccharomyces cerevisiae. To deduce Stk2 function in S. turcica and to identify the genetic relationship between STK2 and KSS1/FUS3 from S. cerevisiae, a restructured vector containing the open reading frame of STK2 was transformed into a fus3/kss1 double deletion mutant of S. cerevisiae. The results show that the STK2 complementary strain clearly formed pseudohyphae and ascospores, and the strain grew on the surface of the medium after rinsing with sterile water and the characteristics of the complementary strain was the same as the wild-type strain. Moreover, STK2 complemented the function of KSS1 in filamentation and invasive growth, as well as the mating behavior of FUS3 in S. cerevisiae, however, its exact functions in S. turcica will be studied in the future research.  相似文献   

野牡丹采穗圃建立和扦插繁殖技术研究     

宋小军  吴宗兴  梁颇  郭洪英  郭慧  李丽梅  张梅 《四川林业科技》2007,28(4):55-60

经过2年来对野牡丹采穗圃的建立和管理及穗条的不同药剂浓度、不同扦插时间、穗条所处不同部位、不同的修剪方式试验研究,结果表明:(1)苗圃中选择的优树建立的采穗圃当年每株母树采穗量达18条～54条,丛生性母株留低桩1 cm～2 cm采穗量最大,达到54条/株;(2)穗条用200 mg.L-1GGR6生根粉处理后的扦插效果最好,生根率达到88.5%,清水处理的穗条扦插效果最差,生根率为68.5%;10月中旬是扦插野牡丹的最佳时间,成活率可达90.5%;枝条上部的穗条扦插成活率最高,可达91.5%;穗条的不同修剪方式对扦插成活率影响不明显,平均成活率80.4%。  相似文献   

杜仲组织培养的研究   总被引：2，自引：0，他引：2  

何泼  申延  秦俊哲  董文宾 《林业科技开发》2006,20(3):27-30

以杜仲无菌苗的幼茎为外植体接种于MS培养基上,并附加相应的植物生长调节剂进行杜仲愈伤组织的诱导和增殖的研究;另外,对不同培养基、光照条件、pH值等理化因子对杜仲愈伤组织增殖的影响进行了研究。结果表明,MS培养基(0．8％琼脂 3％蔗糖)附加2．0 mg／L NAA 0．5 mg／L6-BA有利于杜仲愈伤组织的诱导。B5培养基(0．8％琼脂 3％蔗糖)附加1．0mg／LNAA 1．5mg／L6-BA,并控制pH值为6．5,12 h／d光照有利于愈伤组织的增殖。  相似文献   

促消化功能性韧性饼干的研制   总被引：1，自引：0，他引：1  

邱泼  韩文凤  李喜宏  钟建平 《保鲜与加工》2012,12(4):32-35

鸡内金、陈皮、山楂是具有促消化作用的天然物质。通过正交试验确定三者在韧性饼干中的添加量,研制具有促消化功能的韧性饼干。在基本配方的基础上,确定最佳添加量(以面粉为基准)为:鸡内金1.0%,陈皮2.0%,山楂12.0%,可生产出色泽均匀、形状整齐、薄厚一致、酥松香甜、具有促消化功能的韧性饼干。  相似文献