EARLY DIAGNOSIS OF BORON DEFICIENCY IN CHESTNUT     

Ester Maria Abranches Costa Portela  José Luís Penetra Louzada 《Journal of plant nutrition》2013,36(2):304-310

This study aims to identify an alternative plant tissue to be used in the early diagnosis of boron (B) deficiency in chestnut (Castanea sativa Mill.). A B-deficient orchard was selected, and 16 trees were submitted to two levels of B fertilization. When flowers were in bloom, the following tissues were sampled: leaves, androgynous catkins and flowers. There was a significant increase in B content in plant tissues due to B application. In July, the highest B content was observed in flowers in B0, but leaves had the greatest content in B1. Boron content in the tissues collected in July was positively correlated with B contents in leaves sampled in September. Foliar B concentrations, irrespective of the sampling period, were correlated with chestnut productivity, while the other tissues did not. These results suggest that the leaves, sampled in bloom, were the most efficient tissue for the early diagnosis of B deficiency.  相似文献   

A review of thermal stress in cattle     

RW Shephard  SK Maloney 《Australian veterinary journal》2023,101(11):417-429

Cattle control body temperature in a narrow range over varying climatic conditions. Endogenous body heat is generated by metabolism, digestion and activity. Radiation is the primary external source of heat transfer into the body of cattle. Cattle homeothermy uses behavioural and physiological controls to manage radiation, convection, conduction, and evaporative exchange of heat between the body and the environment, noting that evaporative mechanisms almost exclusively transfer body heat to the environment. Cattle control radiation by shade seeking (hot) and shelter (cold) and by huddling or standing further apart, noting there are intrinsic breed and age differences in radiative transfer potential. The temperature gradient between the skin and the external environment and wind speed (convection) determines heat transfer by these means. Cattle control these mechanisms by managing blood flow to the periphery (physiology), by shelter-seeking and standing/lying activity in the short term (behaviourally) and by modifying their coats and adjusting their metabolic rates in the longer term (acclimatisation). Evaporative heat loss in cattle is primarily from sweating, with some respiratory contribution, and is the primary mechanism for dissipating excess heat when environmental temperatures exceed skin temperature (~36°C). Cattle tend to be better adapted to cooler rather than hotter external conditions, with Bos indicus breeds more adapted to hotter conditions than Bos taurus. Management can minimise the risk of thermal stress by ensuring appropriate breeds of suitably acclimatised cattle, at appropriate stocking densities, fed appropriate diets (and water), and with access to suitable shelter and ventilation are better suited to their expected farm environment.  相似文献   

Crude glycerin combined with sugar cane silage in lamb diets     

Carlos Alberto Alves de Oliveira Filho  José Augusto Gomes Azevêdo  Gleidson Giordano Pinto de Carvalho  Camilla Flávia Portela Gomes da Silva  Ícaro dos Santos Cabral  Luiz Gustavo Ribeiro Pereira  Larissa Gomes dos Reis  Flávio Moreira de Almeida  Lígia Lins Souza 《Tropical animal health and production》2016,48(2):289-295

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Supplementation with Ca salts of soybean oil interacts with concentrate level in grazing dairy cows: intake,ingestive behavior,and ruminal parameters     

Fernanda Lopes Macedo  Fernanda Batistel  Jonas de Souza  Lucas Jado Chagas  Flávio Augusto Portela Santos 《Tropical animal health and production》2016,48(8):1593-1598

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Genetic structure and diversity of the endangered bath sponge Spongia lamella          下载免费PDF全文

Rocío Pérez‐Portela  Charlotte Noyer  Mikel A. Becerro 《水产资源保护:海洋与淡水生态系统》2015,25(3):365-379

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Reply     

RW GEE 《Australian veterinary journal》2002,80(5):305-305

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Pro‐tumorigenic Effects of Transforming Growth Factor Beta 1 in Canine Osteosarcoma     

R.F. Portela  B.A. Fadl‐Alla  H.C. Pondenis  M.L. Byrum  L.D. Garrett  K.L. Wycislo  L.B. Borst  T.M. Fan 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2014,28(3):894-904

Background

Transforming growth factor beta 1 (TGFβ1) is a pleiotropic cytokine that contributes to reparative skeletal remodeling by inducing osteoblast proliferation, migration, and angiogenesis. Organic bone matrix is the largest bodily reservoir for latent TGFβ1, and active osteoblasts express cognate receptors for TGFβ1 (TGFβRI and TGFβRII). During malignant osteolysis, TGFβ1 is liberated from eroded bone matrix and promotes local progression of osteotropic solid tumors by its mitogenic and prosurvival activities.

Hypothesis

Canine osteosarcoma (OS) cells will possess TGFβ1 signaling machinery. Blockade of TGFβ1 signaling will attenuate pro‐tumorigenic activities in OS cells. Naturally occurring primary OS samples will express cognate TGFβ1 receptors; and in dogs with OS, focal malignant osteolysis will contribute to circulating TGFβ1 concentrations.

Animals

Thirty‐three dogs with appendicular OS.

Methods

Expression of TGFβ1 and its cognate receptors, as well as the biologic effects of TGFβ1 blockade, was characterized in OS cells. Ten spontaneous OS samples were characterized for TGFβRI/II expressions by immunohistochemistry. In 33 dogs with OS, plasma TGFβ1 concentrations were quantified and correlated with bone resorption.

Results

Canine OS cells secrete TGFβ1, express cognate receptors, and TGFβ1 signaling blockade decreases proliferation, migration, and vascular endothelial growth factor secretion. Naturally occurring OS samples abundantly and uniformly express TGFβRI/II, and in OS‐bearing dogs, circulating TGFβ1 concentrations correlate with urine N‐telopeptide excretion.

Conclusions and Clinical Importance

Canine OS cells possess TGFβ1 signaling machinery, potentially allowing for the establishment of an autocrine and paracrine pro‐tumorigenic signaling loop. As such, TGFβ1 inhibitors might impede localized OS progression in dogs.  相似文献   

The use of electrical stimulation to guide epidural and intrathecal needle advancement at the L5‐L6 intervertebral space in dogs     

Pablo E Otero  Natali Verdier  Martin R Ceballos  Lisa Tarragona  Myriam Flores  Diego A Portela 《Veterinary anaesthesia and analgesia》2014,41(5):543-547

ObjectiveTo determine the minimal electrical threshold (MET) necessary to elicit appropriate muscle contraction when the tip of an insulated needle is positioned epidurally or intrathecally at the L_5-6 intervertebral space (phase-I) and to determine whether the application of a fixed electrical current during its advancement could indicate needle entry into the intrathecal space (phase-II) in dogs.Study designProspective, blinded study.AnimalsThirteen (phase-I) and seventeen (phase-II) dogs, scheduled for a surgical procedure where L_5-6 intrathecal administration was indicated.MethodsUnder general anesthesia, an insulated needle was first inserted into the L_5-6 epidural space and secondly into the intrathecal space and the MET necessary to obtain a muscular contraction of the pelvic limb or tail at each site was determined (phase-I). Under similar conditions, in dogs of phase-II an insulated needle was inserted through the L_5-6 intervertebral space guided by the use of a fixed electrical current (0.8 mA) until muscular contraction of the pelvic limb or tail was obtained. Intrathecal needle placement was confirmed by either free flow of cerebrospinal fluid (CSF) or myelography.ResultsThe current required to elicit a motor response was significantly lower (p < 0.0001) when the tip of the needle was in the intrathecal space (0.48 ± 0.10 mA) than when it was located epidurally (2.56 ± 0.57). The use of a fixed electrical stimulation current of 0.8 mA resulted in correct prediction of intrathecal injection, corroborated by either free flow of CSF (n = 12) or iohexol distribution pattern (n = 5), in 100% of the cases.Conclusion and clinical relevanceNerve stimulation may be employed as a tool to distinguish epidural from intrathecal insulated needle position at the L_5-6 intervertebral space in dogs. This study demonstrates the feasibility of using an electrical stimulation test to confirm intrathecal needle position in dogs.  相似文献   

Lumbosacral plexus block in a goat undergoing pelvic limb amputation     

Sarah G. Shippy  Marta Romano  Diego A. Portela 《Veterinary anaesthesia and analgesia》2021,48(1):151-152

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A serological survey of Eperythrozoon ovis in goats and sheep in Tasmania     

RW MASON  A. CORBOULD  P. STATHAM 《Australian veterinary journal》1989,66(4):122-123

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