Sub-lethal levels of carvacrol reduce Salmonella Typhimurium motility and invasion of porcine epithelial cells     

Inamuco J  Veenendaal AK  Burt SA  Post JA  Tjeerdsma-van Bokhoven JL  Haagsman HP  Veldhuizen EJ 《Veterinary microbiology》2012,157(1-2):200-207

The European ban on the use of antibiotic growth promotors has increased the search for new alternatives to prevent pig intestinal microbial diseases and to stimulate growth. The addition of essential oils or components thereof, such as carvacrol, to pig feed is a promising alternative. In this report we determined the effect of sub-lethal concentrations of carvacrol on Salmonella Typhimurium. At concentrations where growth of Salmonella was not inhibited, carvacrol completely inhibited motility of the bacterium. This loss of motility was not due to the loss of the flagellum or to ATP shortage upon carvacrol treatment. Adhesion of Salmonella to IPEC-J2, porcine intestinal epithelial cells, was not affected by carvacrol but invasion was significantly reduced. In addition, the epithelial gene expression of porcine β-defensin 2, an innate immune response to Salmonella infection, was reduced when Salmonella was exposed to carvacrol. This indicates that invasion but not adhesion of Salmonella triggers the porcine β-defensin 2 expression of porcine epithelial cells.  相似文献   

An outbreak of the conjunctival form of infectious bovine rhinotracheitis.     

W C Rebhun  J S Smith  J E Post  H R Holden 《The Cornell veterinarian》1978,68(3):297-301

An outbreak of severe conjunctivitis involved approximately 30% of a dairy herd of 118 cattle. Infectious Bovine Rhinotracheitis (IBR) virus was identified by cytopathic effects and fluorescent antibody techniques from conjunctival swabs of affected animals. The disease typically presented as a unilateral ocular disease with systemic signs of pyrexia, inappetance, decreased milk production, and some subsequent abortions.  相似文献   

Evaluation of a test for Clostridium difficile toxins A and B for the diagnosis of neonatal swine enteritis.     

Karen W Post  B Helen Jost  J Glenn Songer 《Journal of veterinary diagnostic investigation》2002,14(3):258-259

A commercially available 1-hour enzyme immunoassay (EIA) for detecting the presence of Clostridium difficile toxins A and B was evaluated for use in diagnosis of C. difficile infections in neonatal swine. This test was compared with a tissue culture cytotoxicity assay, which is considered to be the reference standard for the detection of C. difficile toxins. Twenty-seven samples of colonic contents and 23 fecal samples were collected from freshly euthanized neonatal swine with a history of scours. Of the 50 specimens tested, 20 were positive by the EIA test and tissue culture and 24 were negative by both tests, for an overall correlation of 88%. The sensitivity and specificity of the EIA were 91% and 86%, respectively, and the positive and negative predictive values were 84% and 86%, respectively. The EIA test is considered suitable as an aid for the diagnosis of C. difficile enteritis because of the high correlation between EIA results and those of the tissue culture cytotoxicity assay.  相似文献   

Topical Treatment of Experimental Ringworm in Guinea Pigs with Griseofulvin in Dimethylsulfoxide          下载免费PDF全文

K. Post  J. R. Saunders 《The Canadian veterinary journal. La revue veterinaire canadienne》1979,20(2):45-48

The topical administration of 1.5% griseofulvin in dimethylsulfoxide for five to seven days was a satisfactory treatment regime for experimental ringworm in the guinea pig.  相似文献   

De invloed van de temperatuur op het tijdstip van verpoppen en de duur van het popstadium vanEnarmonia pomonella L.     

J. J. Post  D. J. de Jong 《European journal of plant pathology / European Foundation for Plant Pathology》1958,64(2):130-141

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Aging. Antiaging research and the need for public dialogue     

Juengst ET  Binstock RH  Mehlman MJ  Post SG 《Science (New York, N.Y.)》2003,299(5611):1323

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A high-throughput DNA extraction method for barley seed     

Rebecka von Post  Lars von Post  Christophe Dayteg  Marie Nilsson  Brian P. Forster  Stine Tuvesson 《Euphytica》2003,130(2):255-260

A non-destructive, quick DNA extraction method for barley seed is described. The method is simple and consists of drilling out a sample from the seed, adding sodium hydroxide, heating in a microwave oven and neutralizing with Tris-HCl. The seed DNA extract can be used directly for PCR with extra cycles added to the PCR programme compared to PCR programmes used for leaf extracts. This protocol was developed in particular for a micro satellite marker genetically linked to barley yellow mosaic virus resistance, but it can be applied toother markers of interest for barley breeding. The quick seed extraction protocol makes it possible to handle thousands of samples per day. Extraction of DNA from seed also facilitates transfer of plant material compared to the long-distance transfer of leaf samples. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Alternatives for large-scale production of cultured beef:A review     

Matilda S M Moritz  Sanne E L Verbruggen  Mark J Post 《中国农业科学(英文版)》2015,(2):208-216

Cultured beef is a method where stem cells from skeletal muscle of cows are cultured in vitro to gain edible muscle tissue. For large-scale production of cultured beef, the culture technique needs to become more efficient than today’s 2-dimensional(2D) standard technique that was used to make the first cultured hamburger. Options for efficient large-scale production of stem cells are to culture cells on microcarriers, either in suspension or in a packed bed bioreactor, or to culture aggregated cells in suspension. We discuss the pros and cons of these systems as well as the possibilities to use the systems for tissue culture. Either of the production systems needs to be optimized to achieve an efficient production of cultured beef. It is anticipated that the optimization of large-scale cell culture as performed for other stem cells can be translated into successful protocols for bovine satellite cells resulting in resource and cost efficient cultured beef.  相似文献   

2011年全国森林防火工作要点     

国家森林防火指挥部办公室 《森林防火》2011,(1)

2011年全国森林防火工作的总体要求是:以科学发展观为指导,深入贯彻党的十七届五中全会、中央经济和农村工作会议精神,按照全国林业厅局长和全国森林公安森林防火工作会议部署要求,紧紧围绕生态建设为主的林业发展战略和"双增"目标,积极推进依法治火、科学防火、预警响应、应急处置和基础保障"五大"能力建设,严防重大、特别重大森林火灾,为发展现代林业、建设生态文明、推动科学发展作出新的贡献.  相似文献   

Treatment of Animal Toxicoses: A Regulatory Perspective     

Susan J. Bright DVM  Michael J. Murphy DVM  JD  PhD  Janice C. Steinschneider MA  JD  Randall A. Lovell DVM  PhD  Lynn O. Post DVM  PhD  CAPT  USPHS 《Veterinary Clinics of North America: Food Animal Practice》2011,27(2):481-512

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