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521.
Powell JW 《Science (New York, N.Y.)》1887,9(229):612-614
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A. H. Marshall A. Williams M. T. Abberton T. P. T. Michaelson-Yeates H. G. Powell 《Grass and Forage Science》2003,58(1):63-69
Abstract The dry matter (DM) production of Trifolium repens, T. ambiguum and the backcross 1 (BC1) and backcross 2 (BC2) hybrids with T. repens as the recurrent parent were compared in mixtures with an intermediate heading variety of perennial ryegrass (Lolium perenne L.) under a cutting‐only management over 3 harvest years. Plots of parental legume species and backcross hybrids were established from small plantlets and oversown with the companion grass. In the first harvest year, the DM yield of clover in T. repens plots was greater than that in the BC2 plots and both greater than in the BC1 plots, whilst in the second and third harvest years differences between the DM yield of clover in the T. repens and the BC1 and BC2 hybrid plots were small. Similar results were obtained for the DM yield of total herbage. There were also differences in seasonal growth in the first harvest year, when yield of clover in T. repens plots was greater than in the BC1 and BC2 hybrid plots at early cuts but not at later cuts. Few differences in seasonal growth were observed between parental species and hybrids in subsequent harvest years. Comparison of above‐ and below‐ground biomass showed more DM in roots and rhizome of clover in the backcrosses than in the T. repens plots in the second harvest year but differences were less evident in the third harvest year. The clover in the backcross hybrid plots also had fewer stolon growing points per quadrat than the T. repens plots, but the BC2 had more than the BC1 plots. The exploitation of these hybrids in breeding programmes as a strategy to improve the persistence and drought tolerance of white clover is discussed and implications for forage production considered. 相似文献
524.
Levy-Booth DJ Campbell RG Gulden RH Hart MM Powell JR Klironomos JN Pauls KP Swanton CJ Trevors JT Dunfield KE 《Journal of agricultural and food chemistry》2008,56(15):6339-6347
Glyphosate-tolerant, Roundup Ready (RR) soybeans account for about 57% of all genetically modified (GM) crops grown worldwide. The entry of recombinant DNA into soil from GM crops has been identified as an environmental concern due to the possibility of their horizontal transfer to soil microorganisms. RR soybeans contain recombinant gene sequences that can be differentiated from wild-type plant and microbial genes in soil by using a sequence-specific molecular beacon and real-time polymerase chain reaction (PCR). A molecular beacon-based real-time PCR system to quantify a wild-type soybean lectin ( le1) gene was designed to compare amounts of endogenous soybean genes to recombinant DNA in soil. Microcosm studies were carried out to develop methodologies for the detection of recombinant DNA from RR soybeans in soil. RR soybean leaf litterbags were imbedded in the soil under controlled environmental conditions (60% water holding capacity, 10/15 degrees C, and 8/16 h day/night) for 30 days. The soybean biomass decomposition was described using a single-phase exponential equation, and the DNA concentration in planta and in soil was quantified using real-time PCR using sequence-specific molecular beacons for the recombinant cp4 epsps and endogenous soybean lectin ( le1) genes. The biomass of RR soybean leaves was 8.6% less than nontransgenic (NT) soybean leaves after 30 days. The pooled half-disappearance time for cp4 epsps and le1 in RR and of le1 in NT soybean leaves was 1.4 days. All genes from leaves were detected in soil after 30 days. This study provides a methodology for monitoring the entry of RR and NT soybean DNA into soil from decomposing plant residues. 相似文献
525.
Loma salmonae infections of salmonids culminate in the development of branchial xenomas and subsequent focal hyperplasia of the lamellar or filament epithelium following xenoma rupture and spore release. The effects of this acute branchial disruption upon net ionic flux rates and plasma electrolyte concentrations were determined in juvenile rainbow trout given an experimental oral exposure to L. salmonae. Mean numbers of branchial xenomas peaked at week 5 post-exposure (PE), which coincided with a reduction in the specific growth rate, although there were no significant differences in mass, length or condition of Loma-exposed fish compared with unexposed controls. Following exposure, negative net whole body Na(+) and K(+) fluxes decreased, whereas net Cl(-) fluxes remained unchanged compared with non-exposed control fish. At week 3 PE during the initial branchial xenoma formation stage, there was a significant negative whole body net K(+) flux in Loma-exposed trout compared with other points during the exposure and subsequent infection. Additionally, Loma-exposed fish had marginally elevated plasma Na(+) and Cl(-) concentrations, whilst K(+) levels remained unchanged, compared with control fish. Although there was a progressive decrease in leucocrit, haematocrit remained unchanged over the course of the Loma exposure and subsequent infection. These results suggest that ionic compensation can occur at the gills during the development of xenomas during exposure to L. salmonae and the resultant infection, therefore allowing defence of plasma electrolyte concentrations, unlike the acute ionic disturbances seen with some other parasitic diseases. 相似文献
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527.
Effects of supplemental dietary vitamin C on quality of semen from Nile tilapia (Oreochromis niloticus) breeders
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NLAF Sarmento EFF Martins DC Costa WS Silva CC Mattioli MR Luz RK Luz 《Reproduction in domestic animals》2017,52(1):144-152
The objective of this study was to evaluate the effects of vitamin C on growth and quality of semen from Oreochromis niloticus breeders. One hundred and sixty males were fed with different levels of vitamin C (0, 261, 599 and 942 mg/kg diet). The higher weight values were recorded for 599 (166 g) and 942 (175 g) mg of vitamin C/kg diet. Sperm motility, vigour and concentration were higher with 599 and 942 mg of vitamin C/kg diet. The semen volume, gonadosomatic index and plasma protein data from the last week showed a direct relationship with increasing levels of vitamin C. No changes were observed in the hepatosomatic index and blood glucose. The haematocrit and erythrocyte showed higher values estimated by equations derived at 850 and 638 mg vitamin C/kg diet, respectively. The leucocytes were inversely proportional to the increasing levels of vitamin C. After 100 days of feeding, animals fed the diet containing 942 mg vitamin C/kg diet had higher sperm motility, linearity, curvilinear velocity, straight line velocity and average path velocity (p < .05). Higher values of beat cross‐frequency were observed in broodfish fed diets containing 942 and 599 mg vitamin C/kg. The different vitamin C levels did not cause differences in straightness, lateral head displacement and sperm morphology. For Nile tilapia males on intensive rearing and handling conditions, vitamin C levels between 599 and 942 mg/kg may be used for a better performance and quality of semen. 相似文献
528.
Kim C. Steiner Jared W. Westbrook Frederick V. Hebard Laura L. Georgi William A. Powell Sara F. Fitzsimmons 《New Forests》2017,48(2):317-336
Following the near-obliteration of American chestnut (Castanea dentata [Marsh.] Borkh.) by the chestnut blight early in the last century, interest in its restoration has been revived by efforts to develop a blight-resistant form of the species. We summarize progress and outline future steps in two approaches: (1) a system of hybridizing with a blight-resistant chestnut species and then backcrossing repeatedly to recover the American type and (2) transformation of American chestnut with a resistance-conferring transgene followed by propagation and conventional breeding. Several decades of effort have been invested in each approach. More work remains, but results indicate that success is within practical reach. The restoration of C. dentata to its native habitat now appears to be less a matter of time and conjecture than ever before in 90 years of work by public and private entities. The difficult and protracted task of incorporating extraspecific genes for resistance into a tree species with lethal susceptibility to a naturalized pathogen represents perhaps the most extreme of restoration challenges. Its pursuit by a small non-governmental organization supported primarily by philanthropy and volunteers may serve as a model for other species threatened by exotic pathogens or insects. 相似文献