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To tackle the growing problem of antibiotic resistance, it is essential to identify new bioactive compounds that are effective against resistant microbes and safe to use. Natural products and their derivatives are, and will continue to be, an important source of these molecules. Sea sponges harbour a diverse microbiome that co-exists with the sponge, and these bacterial communities produce a rich array of bioactive metabolites for protection and resource competition. For these reasons, the sponge microbiota constitutes a potential source of clinically relevant natural products. To date, efforts in bioprospecting for these compounds have focused predominantly on sponge specimens isolated from shallow water, with much still to be learned about samples from the deep sea. Here we report the isolation of a new Micromonospora strain, designated 28ISP2-46T, recovered from the microbiome of a mid-Atlantic deep-sea sponge. Whole-genome sequencing reveals the capacity of this bacterium to produce a diverse array of natural products, including kosinostatin and isoquinocycline B, which exhibit both antibiotic and antitumour properties. Both compounds were isolated from 28ISP2-46T fermentation broths and were found to be effective against a plethora of multidrug-resistant clinical isolates. This study suggests that the marine production of isoquinocyclines may be more widespread than previously supposed and demonstrates the value of targeting the deep-sea sponge microbiome as a source of novel microbial life with exploitable biosynthetic potential.  相似文献   
94.
ABSTRACT

Most of the sugarcane (interspecific hybrids of Saccharum sp.) production in Florida is on organic soils. Supplemental phosphorus (P) fertilizer is often applied for optimum yields, but producers are required to reduce P levels in farm drainage waters. The objectives of this study were to relate optimum leaf P tissue concentration with yield in organic soil, and to determine optimum leaf sampling dates during the summer. Eight genotypes were planted at two locations, eight additional genotypes were planted at a third location, and eight more genotypes were planted at a fourth location. Crops were grown for three years. Measurements of leaf P concentration were repeated during growth seasons and over crop years for a total of six sampling dates at each location. Three fertilizer P treatments (0, 24, and 48 kg ha?1 yr?1) were applied to all genotypes at each location. Leaf samples were partitioned into early-, mid-, and late-summer dates. Early-leaf samples had the widest range in leaf P concentrations compared with mid- and late-season leaf samples. Correlation analyses of yield vs. leaf P concentration across all treatments in early- and mid-summer were statistically significant (P < 0.05), but coefficients were low (r = 0.14 and 0.26, respectively). No consistent relationship across locations described the effect of leaf P tissue concentration on yield. Leaf P concentrations could not provide accurate P fertilization rates that will give maximum sugarcane yields and prevent over-fertilization of P. The highest potential for relating leaf P concentrations with yield appears to be from early leaf samples.  相似文献   
95.
Populations of the green peach aphid,Myzus persicae (Sulzer), and the potato aphid,Macrosiphum euphorbiae (Thomas), were observed on 27 named varieties of Irish potatoes in the Yakima Valley of Washington intermittently from 1947 through 1968. The Epicure, Great Scot, and B5141-6 varieties produced relatively fewer green peach aphids than other varieties tested; Bliss Triumph and Red Pontiac produced relatively fewer potato aphids. Except for Doebay Red, the red-skinned varieties, Bliss Triumph, Red McLure, Norland, and Earnly Rose, were extremely to moderately productive of the green peach aphid. No correlation was apparent between abundance of aphids and the specific gravity or yield of tubers.  相似文献   
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Summary The chloroplast DNA of 27 potato cultivars and 2 wild species has been examined by Southern blotting using probes representing the entire chloroplast genome of barley. 16% of the probe × enzyme combinations detected polymorphism between the potato cultivars. Cvs Maris Piper, Croft, Shelagh, Stormont Enterprise and Estima and the wild species could be distinguished from the other cultivars by their chloroplast DNA restriction patterns. These results are discussed in relation to cytoplasmic diversity and potato improvement.  相似文献   
98.
Webb  Hailey  Barnes  Nathaniel  Powell  Scott  Jones  Clain 《Precision Agriculture》2021,22(6):1803-1815
Precision Agriculture - Soil acidification is a growing problem in semi-arid agroecosystems. In the state of Montana, USA, soil pH levels below 5.5 have been documented in nearly half of the...  相似文献   
99.
Examples of equine disease surveillance at the local, regional, national and international level are discussed in this article. Reporting systems at each level are also considered, and the increasing importance of laboratory confirmed diagnoses is emphasized. The need to develop national disease reporting systems is addressed, particularly with respect to conforming to international trading standards.  相似文献   
100.
It is likely that in the near future sequence information from sequencing programmes and EST libraries will generate an abundance of genic microsatellite markers. This study is focused on the assessment of their likely impact and performance vis-à-vis their genomic counterparts. Microsatellites from two sources were used to assess the genetic diversity in 56 old and new varieties of bread wheat on the UK Recommended List. A set of 12 microsatellite markers generated from genomic libraries and 20 expressed sequence tag (EST)-derived microsatellites were used in the study, and the performance of both marker sets assessed. The EST-derived or genic microsatellites delivered fingerprints of superior quality, amplifying clear products with few stutter bands. Diversity levels as revealed bygenic microsatellites are similar to the few published results. The PIC values for the genic markers were generally lower than those calculated for the genomic microsatellites, though advantages of both marker classes for variety identification applications are discussed. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   
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