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21.
Lakshi A. Dayarathne Sachithra S. Ranaweera Premkumar Natraj Priyanka Rajan Young Jae Lee Chang-Hoon Han 《Journal of veterinary science (Suw?n-si, Korea)》2021,22(4)
BackgroundNaringenin and its glycoside naringin are well known citrus flavonoids with several therapeutic benefits. Although the anti-adipogenic effects of naringenin and naringin have been reported previously, the detailed mechanism underlying their anti-adipogenesis effects is poorly understood.ObjectivesThis study examined the anti-adipogenic effects of naringenin and naringin by determining differential gene expression patterns in these flavonoids-treated 3T3-L1 adipocytes.MethodsLipid accumulation and triglyceride (TG) content were determined by Oil red O staining and TG assay. Glucose uptake was measured using a 2-[N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-d-glucose fluorescent d-glucose analog. The phosphorylation levels of AMP-activated protein kinase (AMPK) and acetyl Co-A carboxylase (ACC) were observed via Western blot analysis. Differential gene expressions in 3T3-L1 adipocytes were evaluated via RNA sequencing analysis.ResultsNaringenin and naringin inhibited both lipid accumulation and TG content, increased phosphorylation levels of both AMPK and ACC and decreased the expression level of 3-hydroxy-3-methylglutaryl CoA reductase (HMGCR) in 3T3-L1 adipocytes. RNA sequencing analysis revealed that 32 up-regulated (> 2-fold) and 17 down-regulated (< 0.6-fold) genes related to lipid metabolism, including Acaca, Fasn, Scd1, Mogat1, Dgat, Lipin1, Cpt1a, and Lepr, were normalized to the control level in naringenin-treated adipocytes. In addition, 25 up-regulated (> 2-fold) and 25 down-regulated (< 0.6-fold) genes related to lipid metabolism, including Acaca, Fasn, Fabp5, Scd1, Srebf1, Hmgcs1, Cpt1c, Lepr, and Lrp1, were normalized to the control level by naringin.ConclusionsThe results indicate that naringenin and naringin have anti-adipogenic potentials that are achieved by normalizing the expression levels of lipid metabolism-related genes that were perturbed in differentiated 3T3-L1 cells. 相似文献
22.
Six rice cultivars viz. PR120, PR116, Feng Ai Zan, PR115, PAU201 and Punjab Mehak 1 under the direct-seeded and transplanted conditions were used to investigate the involvement of antioxidative defence system in relation to polyamine catabolism in temporal regulation of developing grains. Activities of ascorbate peroxidase(APx), guaiacol peroxidase(GPx), catalase(CAT), superoxide dismutase(SOD), polyamine oxidases(PAO) and contents of ascorbate, α-tocopherol, proline and polyamines increased gradually until mid-milky stage and then declined towards maturity stage under both planting conditions. The transplanted condition led to higher activities of antioxidative enzymes(APx, GPx and CAT) and contents of ascorbate, α-tocopherol and proline whereas the direct-seeded condition had elevated levels of PAO and SOD activities and contents of polyamines, lipid peroxide and hydrogen peroxide. Cultivars Feng Ai Zan and PR120 exhibited superior tolerance over other cultivars by accumulating higher contents of ascorbate, α-tocopherol and proline with increasing level of PAO and SOD activities under the direct-seeded condition. However, under the transplanted condition PR116 and PAU201 showed higher activities of antioxidative enzymes with decreasing content of lipid peroxide. Therefore, we concluded that under the direct-seeded condition, enhancements of polyamines content and PAO activity enabled rice cultivars more tolerant to oxidative stress, while under the transplanted condition, antioxidative defence with decreasing of lipid peroxide content was closely associated with the protection of grains by maintaining membrane integrity during rice grain filling. The results indicated that temporal dynamics of H2O2 metabolic machinery was strongly up-regulated especially at the mid-milky stage. 相似文献
23.
EST‐SSR analysis provides insights about genetic relatedness,population structure and gene flow in grass pea (Lathyrus sativus) 
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下载免费PDF全文 Khela R. Soren Ashutosh Yadav Gaurav Pandey Priyanka Gangwar Ashok K. Parihar Abhishek Bohra Girish P. Dixit Subhojit Datta Narendra Pratap Singh 《Plant Breeding》2015,134(3):338-344
Grass pea (Lathyrus sativus) is an important food‐legume crop for resource‐poor farmers in the developing world. However, given its cultivation in the most underprivileged regions, the crop has not received appropriate scientific attention particularly from the genomic perspective, thereby giving it a status of genomic orphan. Nevertheless, some recent studies have attempted to develop modern molecular tools to strengthen the genetic and genomic research. In the present investigation, a comprehensive collection comprising 176 accessions was analysed using EST‐simple sequence repeats (SSRs). The SSR analysis revealed existence of a total of 51 alleles with an average polymorphic information content value of 0.35. A moderate level of gene diversity was noticed that ranged from 0.04 to 0.73 with an average of 0.43. Noticeably, two distinct subpopulations were recovered using cluster analysis. In addition, the presence of admixtures in population reflected the strong possibilities of gene flow between the accessions across the geographical boundary. In summary, we provide additional insights about the informativeness of available EST‐SSR markers along with an extended understanding of relatedness, genetic structure and gene flow in an under‐researched legume crop. 相似文献
24.
Narpinder Singh Priyanka Paul Amardeep Singh Virdi Parmeet Kaur Gulshan Mahajan 《Cereal Chemistry》2014,91(4):389-397
Milled rice of four indica cultivars (PR114, PR116, PR118, and PAU201) obtained from early (June 5 and 15) and delayed (June 25 and July 5) transplanted paddy was evaluated for physicochemical, pasting, cooking, and protein characteristics. During the grain‐filling and development stage, early transplanted paddy experienced average maximum and minimum temperatures of 32.8 and 22.3°C, respectively, against 30.8 and 15°C, respectively, for delayed transplanted paddy. Lightness (L*) value, protein content, and lipid content of milled rice were lower, whereas apparent amylose content, cooked grain hardness, final viscosity, and setback viscosity were higher in the delayed transplanted paddy. Whiteness of milled rice of PR114, PR116, and PR118 from delayed transplanted paddy was lower than milled rice from early transplanted paddy. PAU201 did not show any change in whiteness with delayed transplantation. Cooked rice of PAU201 showed exceptionally higher adhesiveness and lower hardness compared with other cultivars. Protein profiling showed a lower accumulation of prolamines in milled rice from PR114 and PAU201 upon delaying the transplantation from June 5 to July 5, whereas PR116 and PR118 showed a decrease in prolamine accumulation from transplantation on June 25. The changes in milled rice caused by delayed transplanting of paddy might be because of cool nighttime air temperature that affected the accumulation of starch, protein, and lipid. 相似文献
25.
Tripathi Rahul Nayak Amaresh Kumar Dhal Biswaranjan Shahid Mohammad Lal Banwari Gautam Priyanka 《Archives of Agronomy and Soil Science》2013,59(13):1775-1787
ABSTRACTThe present study was to delineate management zones (MZs) in salt affected Mahakalpada block in eastern India by capturing both spatial variability of soil parameters along with satellite derived Normalized Difference Vegetation Index (NDVI) and Enhanced Vegetation Index (EVI). Grid wise 237 soil samples collected from the study area were analyzed and spatial maps were generated for physicochemical properties, DTPA extractable micronutrients, i.e. iron, zinc, copper, and manganese and major nutrients, i.e. available nitrogen (AN), phosphorous (AP), and potassium (AK). Soil electrical conductivity and AK showed a high CV of 100% and 56.7%, respectively. Principal component analysis was performed using the soil spatial maps, NDVI and EVI maps and only four principal components which produced eigenvalues > 1 and accounting for 75.4% of the total variability were retained for further analysis. Further, fuzzy c-mean clustering was used to delineate the MZs based on fuzzy performance index (FPI) and normalized classification entropy (NCE) was used for identifying the three MZs. There was a significant difference between MZ1 and MZ2 for all the variables except AN and EVI whereas all the variables were significantly different between MZ1 and MZ3 highlighting the usefulness of MZs delineation technique for site-specific nutrient management. 相似文献
26.
Genetic Resources and Crop Evolution - The genus Allium is one of the largest monocotyledon genera having 1000 designated species with commercial and economic significance. Allium cepa L. (onion)... 相似文献
27.
Priyanka Suryavanshi Y. V. Singh R. Prasanna Arti Bhatia Y. S. Shivay 《Paddy and Water Environment》2013,11(1-4):321-329
Methane (CH4) emission and water productivity were estimated in an experiment conducted during wet (rainy) season of 2010 at the research farm of Indian Agricultural Research Institute, New Delhi, India. Treatments comprising three methods of crop establishment viz., conventional transplanting (CT), system of rice intensification (SRI) and double transplanting (DT) were laid out in randomized block design with four replications. Scented rice (Oryza sativa L) variety ‘Pusa Basmati 1401’ was transplanted in puddle field. In CT and SRI 21 and 12-day-old seedlings, respectively, were transplanted while in DT overall 45-day-old seedlings were transplanted. In CT and DT flooded conditions while in SRI saturated conditions were maintained. Results indicated that among the methods of crop establishment, CT had maximum cumulative CH4 emission (32.33 kg ha?1) followed by DT (29.30 kg ha?1) and SRI (19.93 kg ha?1). Temporal CH4 flux fluctuated between 79.7 and 482.0 mg m?2 day?1 under CT; 46.0 and 315.0 mg m?2 day?1 in SRI and 86.7 and 467.3 mg m?2 day?1 in DT. Considerable temporal variations in the individual CH4 fluxes were observed. Flux of CH4 was generally higher in early stage of crop and peaked about 21 days after transplanting coinciding with tillering stage of crop. CH4 flux declined gradually from 75 days after transplanting and stabilized at the harvest stage of rice in all the three methods of transplanting. Global warming potential was highest in CT (807.4 kg CO2 ha?1) and lowest in SRI (498.25 kg CO2 ha?1). However, a reverse trend was observed with carbon efficiency ratio. The water savings to the extent of six irrigations was recorded in SRI over CT. A saving of 27.4 % irrigation water and 18.5 % total water was recorded in SRI over CT while the corresponding values of DT over CT were 14.5 and 9.8 %. Water productivity of SRI (3.56 kg/ha mm) was significantly higher as compared to DT (2.87 kg/ha mm) and CT (2.61 kg/ha mm). 相似文献
28.
Prashant Kumar MishraRavindra Shukla Priyanka SinghBhanu Prakash Nawal Kishore Dubey 《Industrial Crops and Products》2012,36(1):74-80
The study deals with evaluation of antifungal and antiaflatoxigenic Caesulia axillaris Roxb. essential oil (EO) against herbal raw materials deteriorating fungi and its free radical scavenging activity. During mycoflora analysis these herbal raw materials were found to be severely contaminated by different fungi and aflatoxins. A total of nine different fungal species were isolated from three herbal raw materials. Aspergillus flavus LHPtc was recorded as the highest aflatoxin B1 producing strain. EOs of some plants were tested for their fungitoxicity against the toxigenic strain A. flavus LHPtc, and C. axillaris EO was found as potent fungitoxicant. C. axillaris EO was chemically characterized through GC-MS analysis which depicted the presence of 18 compounds, dl-limonene and Euasarone being the major components. The EO exhibited broad spectrum of fungitoxicity against fungi causing postharvest deterioration of herbal raw materials. At 1.0 μl ml−1 the oil showed complete inhibition of fungal growth and aflatoxin B1 production was inhibited at 0.8 μl ml−1. Free radical scavenging activity of the oil was also recorded by 2,2-diphenyl-1-picrylhydrazyl assay, and its IC50 value was found 18 μl ml−1. The safety limit of the EO was determined in terms of LD50 on mice, which was 9166.6 μl kg−1, suggesting its non mammalian toxicity. The EO of C. axillaris may be recommended as a plant based preservative in enhancement of shelf life of herbal raw materials by preventing their lipid peroxidation as well as biodeterioration due to fungal and aflatoxin contamination. 相似文献
29.
Most of the natural dyes require mordants for their fixation on textile materials. Natural mordants are thus gaining importance in order to get complete-natural dyeing. In the present work, natural dyes and mordants were extracted using conventional and ultrasound methods and comparative studies were made. The natural mordants namely harda and tamarind seed coat and natural dyes like turmeric, henna were extracted using conventional and ultrasound approaches and various extracts obtained were described in terms of their optical densities. In order to verify the extraction efficiencies, wool fabrics were dyed with extracts of various combinations of mordants/dyes and dyed fabrics were evaluated for their colour strengths and fastness properties. The extent of colour extraction was higher in case of ultrasound assisted extraction as compared to that of conventional method. The fabrics dyed using extracts of ultrasound method showed higher colour values as compared to those dyed using extracts from conventional methods thus confirming ultrasound as more efficient method of extraction. 相似文献
30.
José Ruiz Hidalgo Melina Santillán Eduardo Alberto Parellada Priyanka Khyaliya Adriana Neske 《国际虫害防治杂志》2020,66(2):116-121
AbstractThis is the first report on the antifeedant, toxic and nutritional effects produced by the synthetic chalcones on the lepidopteran Spodoptera frugiperda followed by structure-activity relationship. Bis (5a-c) and mono (8a-d) chalcones have been synthesized via Claisen-Schmidt condensation and have been purified and characterized. The mono chalcones 8b and 8d displayed toxic effects on early larval instars when incorporated to the larval diet at 100?µg per g of diet (40 and 60%, respectively) and low adults emergency with malformations leading to death. The remaining chalcones 8a, 8c, 5a, 5b and 5c do not displayed toxic effects at the same dose. The evaluation of nutritional indices indicated that 8a and 8c were the only tested chalcones to produce decrease in the growth rate and to reduce the efficiency with which larvae converted ingested food into biomass. 相似文献