Construction and evaluation of introgression lines and fine mapping of ehd8 from Jinghong common wild rice (Oryza rufipogon)     

Dayun Sun  Wenxiang Wang  Xiaoyun Xin  Liming Cao  Xuejun Sun  Zejun Hu  Ling Jiang  Shiqing Dong  Yahui Liu  Jinshui Yang  Xiaojin Luo 《Plant Breeding》2019,138(2):163-173

Heading date is one of the most important traits in rice and regulated by multiple genes. Common wild rice is the ancestor of Asian cultivated rice and harbours abundant genetic diversity. To use wild rice resource in rice breeding, a set of 154 introgression lines (ILs) covering 93% of the genome of Jinghong common wild rice was constructed in the background 'Teqing', using 208 simple sequence repeat markers evenly distributed on 12 chromosomes. Among the ILs, the line JIL64 displayed late heading independent of photoperiod. Genetic analysis using the two F₂ populations crossed ''Teqing'/JIL64 and JIL64/'Teqing' revealed that late flowering was controlled by a recessive gene on chromosome 8 (designated early heading date 8, ehd8), and ehd8 was fine mapped to the 50‐kb region flanked by markers RM22221 and 64Indel4. Sequencing and qRT‐PCR demonstrated that LOC_Os08g01410 and LOC_Os08g01420 were deleted in JIL64 and may be associated with the late heading of Jinghong common wild rice. These findings lay a practical foundation for characterizing ehd8, and the ILs help to mine genes from Jinghong common wild rice.  相似文献   

浅谈陆地棉杂种二代利用   总被引：1，自引：0，他引：1  

陈亮亮  张梦  郭立平  戚廷香  张学贤  唐会妮  王海林  乔秀琴  吴建勇  邢朝柱 《中国棉花》2020,47(12):1-4

棉花杂种优势利用是提高棉花产量主要途径之一。研究表明，一些陆地棉F₂仍具有明显的产量杂种优势，种子成本低廉的F2具有潜在的应用前景。本文综述了陆地棉F₂杂种优势遗传基础、优势表现以及目前生产上出现的问题，并从育种层面提出了有针对性的解决对策。  相似文献   

不同贮藏年限老芒麦种胚线粒体抗氧化功能的变化规律     

唐嘉  毛春力  黄琪  唐俊  宋玉梅  孙铭  毛培胜 《草地学报》2020,28(5):1226-1232

种子在自然贮藏过程中常常伴随着内部生理机能的恶化，线粒体作为种子内活性氧（reactive oxide species，ROS）产生的主要位点是最先遭到破坏的细胞器。为探讨不同贮藏年限对老芒麦种胚线粒体抗氧化功能的影响，本试验以室温贮藏0～4年的老芒麦种子为材料，分析比较其老化规律及种胚线粒体抗氧化特性的变化规律。结果表明：随着贮藏年限的延长，老芒麦种子发芽势、发芽率和种苗鲜重逐渐下降，死种子逐渐增多，种胚线粒体苹果酸脱氢酶（mitochondria malate dehydrogenase，MDH）、谷胱甘肽还原酶（glutathione reductase，GR）、单脱氢抗坏血酸还原酶（monodehydroascorbate reductase，MDHAR）活性逐渐下降，但在死种子中超氧化物歧化酶（superoxide dismutase，SOD）活性显著升高。此外，在贮藏过程中老芒麦种胚线粒体O₂^·-产生速率不断上升，而H₂O₂含量则呈现逐渐降低的趋势，表明线粒体中O₂^·-的积累与细胞氧化损伤密切相关。  相似文献   

完善内地主产棉区棉花补贴机制的思考与建议——以山东省为例   总被引：1，自引：1，他引：0  

王桂峰  魏学文  孙玮琪  徐勤青  王琰  王安琪 《中国棉花》2017,44(8):8-11

总结了棉花目标价格补贴政策实施以来山东植棉区的实施进展、成效与问题。从国家棉花产业安全和山东省经济社会发展角度，分析了山东植棉区棉花生产的重要地位和保持补贴政策稳定性的重要意义。以山东为例，提出了内地主产棉区棉花补贴政策改革建议。  相似文献   

High water content silk protein-based hydrogels with tunable elasticity fabricated via a Ru(II) mediated photochemical cross-linking method     

Di Zhang  Han Peng  Baochang Sun  Shanshan Lyu 《Fibers and Polymers》2017,18(10):1831-1840

Silk is very promising in the field of biomaterials as a natural biomacromolecule. Silk protein can be made into various forms of materials, including hydrogels. However, silk protein-based hydrogels have not attracted much attention due to its weak mechanical properties. Here, we report high water content silk protein-based hydrogels with tunable elasticity which were fabricated through Ru(II) mediated photochemically cross-linking tyrosine residues in regenerated silk protein. The regenerated silk protein was characterized by Fourier transform infrared spectroscopy (FTIR). The gelation kinetics of the silk protein was studied by rheology measurements. The compressive mechanical properties of the silk protein-based hydrogels was investigated using compressive tests and dynamic mechanical analysis (DMA). Compressive modulus of the hydrogels reached 349±64 MPa at 15 % strain. The fabricated silk protein-based hydrogels were also characterized by Scanning electron microscopy (SEM), revealing an interconnected porous network structure, typical of hydrogels, with an average pore size of approximately 130 μm. Finally, biocompatibility of the silk protein-based hydrogels was demonstrated through cell culture studies using a human fibroblast cell line, HFL1. The reported silk protein-based hydrogels represent a promising candidate for biomaterial applications.  相似文献   

棉花“保险+期货”模式助力南疆主产棉区脱贫攻坚   总被引：1，自引：0，他引：1  

张亚林  吴冬梅  王文魁  程思贤  孙婧  李莉  马小艳 《中国棉花》2020,47(2):6-8

通过解析棉花“保险+期货”模式的运行机制、优势及存在的不足，并提出了发展优质棉“订单生产+保险+期货”模式和引入银行、基金等信贷机构的建议，期望对南疆主产棉区贫困棉农增收致富、助力脱贫攻坚提供有益探索。  相似文献   

体细胞克隆荷斯坦奶牛出生后死亡犊牛内脏器官的组织病理学观察     

孙伟  包向男  乌云高娃  王建国  李永胜  李善铎  张铁柱  王飞飞  李喜和 《中国农学通报》2020,36(20):114-118

为探究体细胞克隆荷斯坦奶牛出生后死亡的原因,对新生后死亡的克隆荷斯坦公牛和自然繁殖的荷斯坦公犊的主要组织器官进行比较。通过解剖和石蜡切片-HE(hematoxylin-eosin staining)染色技术,对主要的组织器官结构进行观察和分析。结果表明,新生后死亡的克隆荷斯坦公牛肺部结构清晰;肝脏的肝细胞明显肿大,出现脂肪轻度变性;肾小管上皮细胞出现变性;心肌的肌纤维间空隙增大;骨骼肌纤维间隙明显,空泡变性,这可能导致该克隆牛犊肌肉无力并功能不全;淋巴结皮髓质界限不分明,淋巴小结细胞较稀疏,生发中心不明显,淋巴窦细胞较少;脾脏内红细胞较少,这说明该克隆牛的造血功能可能不完善;胸腺的皮质部分和髓质部分界限不明显,嗜酸性胸腺小体不易辨认,可能发育不全。该克隆公牛免疫器官出现不同程度的发育不全现象较严重,这有可能是其出生后死亡率高的主要原因。  相似文献   

Effect of Jieyuwan on depression-like behaviors and expression of BDNF in hippocampus and prefrontal cortex of WKY rats     

SONG Miao  LIU Hong-mei  LI Qi  ZHANG Yu  XU Yong 《园艺学报》2019,35(6):1124-1129

AIM:To investigate the mechanism of depression and its development, and to study the expression of brain-derived neurotrophic factor (BDNF) in the hippocampus and prefrontal cortex of Wistar-Kyoto (WKY) rats treated with Jieyuwan. METHODS:Adult male WKY rats were used as an animal model of endogenous depression. Wistar rats of the same strain were selected as control group. WKY rats were randomly divided into model group, citalopram group and Jieyuwan group. After intragastric administration for 21 d, the changes of depression-like behaviors were observed by sucrose preference test and forced swimming test. Immunofluorescence and Western blot were used to detect the expression of BDNF in the hippocampus and prefrontal cortex. RESULTS:WKY rats showed significant depression-like behaviors, and the expression of BDNF was significantly decreased in the hippocampus and prefrontal cortex (P<0.01). The reduction of neuronal axons in hippocampus was also observed. After drug treatment, the depression-like behaviors of WKY rats were significantly attenuated, and the expression of BDNF and the number of axons were increased (P<0.01). CONCLUSION:Jieyuwan effectively attenuates the depression-like behaviors of WKY rats, and BDNF is a key factor in its antidepressant effect. Our findings further confirm the involvement of BDNF in the development of depression.  相似文献   

羊支原体性肺炎两标准株对抗菌药物敏感性的研究     

林密  孙继国  柴静 《猪业科学》2003,20(12):57-59

本实验测定了环丙沙星、氧氟沙星、单诺沙星、红霉素、罗红霉素、泰勒菌素、泰妙菌素、四环素等8种药物对羊肺炎支原体两个标准株Y98和Y-goat的体外抑菌浓度以及红霉素与氧氟沙星、泰勒菌素对Y-goat和四环素与氧氟沙星、泰勒菌素对Y98的联合药敏作用。对羊肺炎支原体两个标准株对抗菌药物的敏感性进行了分析。  相似文献   

预应力薄腹梁质量事故的分析及鉴定     

张国志  王晓军 《东北林业大学学报》1994,22(4):115-119

阐述了某工程由于施工原因，导致预应力混凝土工字形屋面梁强度不足的预制构件的检验鉴定过程和方法。采用回弹法推定混凝土实际强度；通过静载试验测试构件的实际挠度、抗裂和承载能力。分析了导致混凝土强度不足的原因。  相似文献